YPR029C / APL4

Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport

Zygosity: Homozygous strain
fixedexpanded
Profile for YPR029C / APL4

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.50|P-value:1.62E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:3.31|P-value:3.44E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.01|P-value:2.00E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:3.85|P-value:4.05E-5 ID:SGTC_207|Compound:4185-0010|FD-Score:11.60|P-value:2.43E-32 ID:SGTC_221|Compound:trifluoperazine|FD-Score:13.20|P-value:3.17E-41 ID:SGTC_252|Compound:miconazole|FD-Score:6.14|P-value:2.10E-10 ID:SGTC_254|Compound:nsc-64875|FD-Score:3.57|P-value:1.24E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:5.41|P-value:1.77E-8 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:3.67|P-value:8.26E-5 ID:SGTC_435|Compound:c-PAF|FD-Score:-3.26|P-value:6.53E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:6.08|P-value:3.02E-10 ID:SGTC_447|Compound:ml7|FD-Score:3.48|P-value:1.75E-4 ID:SGTC_449|Compound:xestospongin c|FD-Score:4.67|P-value:9.10E-7 ID:SGTC_459|Compound:nigericin|FD-Score:3.68|P-value:7.99E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:5.58|P-value:6.39E-9 ID:SGTC_540|Compound:1187-0821|FD-Score:-4.49|P-value:4.09E-6 ID:SGTC_649|Compound:1123-0311|FD-Score:3.66|P-value:8.70E-5 ID:SGTC_654|Compound:1124-0271|FD-Score:3.11|P-value:6.82E-4 ID:SGTC_689|Compound:0833-0039|FD-Score:4.05|P-value:1.66E-5 ID:SGTC_788|Compound:1182-0090|FD-Score:5.44|P-value:1.46E-8 ID:SGTC_814|Compound:3342-0569|FD-Score:3.17|P-value:5.64E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:4.00|P-value:2.11E-5 ID:SGTC_995|Compound:3931-2021|FD-Score:3.24|P-value:4.38E-4 ID:SGTC_997|Compound:3996-0078|FD-Score:3.81|P-value:4.82E-5 ID:SGTC_1165|Compound:k072-0275|FD-Score:3.58|P-value:1.21E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:3.40|P-value:2.38E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:3.38|P-value:2.63E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:4.05|P-value:1.72E-5 ID:SGTC_1412|Compound:3909-8563|FD-Score:4.15|P-value:1.10E-5 ID:SGTC_1502|Compound:4534-4282|FD-Score:3.21|P-value:4.87E-4 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:3.41|P-value:2.31E-4 ID:SGTC_1676|Compound:st018489|FD-Score:4.11|P-value:1.30E-5 ID:SGTC_1679|Compound:4130-1276|FD-Score:4.04|P-value:1.76E-5 ID:SGTC_1735|Compound:st034310|FD-Score:3.51|P-value:1.58E-4 ID:SGTC_1748|Compound:st037455|FD-Score:-4.44|P-value:5.18E-6 ID:SGTC_1750|Compound:st081859|FD-Score:32.10|P-value:4.14E-230 ID:SGTC_1784|Compound:st039334|FD-Score:3.48|P-value:1.79E-4 ID:SGTC_1894|Compound:3',6'-dihydroxyflavone|FD-Score:-3.56|P-value:2.18E-4 ID:SGTC_1901|Compound:st060216|FD-Score:-3.19|P-value:8.37E-4 ID:SGTC_1911|Compound:st060208|FD-Score:3.16|P-value:5.84E-4 ID:SGTC_1953|Compound:st076800|FD-Score:3.26|P-value:4.03E-4 ID:SGTC_1969|Compound:st069479|FD-Score:-3.56|P-value:2.18E-4 ID:SGTC_1972|Compound:st070201|FD-Score:-4.32|P-value:8.99E-6 ID:SGTC_2006|Compound:4011487|FD-Score:3.21|P-value:4.88E-4 ID:SGTC_2120|Compound:5538279|FD-Score:3.14|P-value:6.22E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.66|P-value:9.55E-7 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.33|P-value:3.12E-4 ID:SGTC_2394|Compound:5221648|FD-Score:3.83|P-value:4.37E-5 ID:SGTC_2484|Compound:5809818|FD-Score:3.25|P-value:4.23E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.60|P-value:1.28E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:7.13|P-value:2.19E-13 ID:SGTC_2584|Compound:androsterone|FD-Score:4.32|P-value:4.97E-6 ID:SGTC_2619|Compound:obliquin|FD-Score:6.00|P-value:5.00E-10 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.56|P-value:1.55E-6 ID:SGTC_2707|Compound:st078556|FD-Score:-3.15|P-value:9.52E-4 ID:SGTC_2837|Compound:9008476|FD-Score:-3.54|P-value:2.29E-4 ID:SGTC_2840|Compound:9008771|FD-Score:-3.14|P-value:9.71E-4 ID:SGTC_2865|Compound:9030313|FD-Score:3.08|P-value:7.69E-4 ID:SGTC_2940|Compound:9060638|FD-Score:4.57|P-value:1.52E-6 ID:SGTC_2951|Compound:9072950|FD-Score:3.03|P-value:9.13E-4 ID:SGTC_2991|Compound:9074669|FD-Score:3.01|P-value:9.82E-4 ID:SGTC_3037|Compound:9091026|FD-Score:4.77|P-value:5.66E-7 ID:SGTC_3156|Compound:9099931|FD-Score:3.28|P-value:3.73E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-3.18|P-value:8.47E-4 ID:SGTC_3311|Compound:9131146|FD-Score:3.37|P-value:2.68E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:3.50|P-value:1.62E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:3.31|P-value:3.44E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.01|P-value:2.00E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:3.85|P-value:4.05E-5 ID:SGTC_207|Compound:4185-0010|FD-Score:11.60|P-value:2.43E-32 ID:SGTC_221|Compound:trifluoperazine|FD-Score:13.20|P-value:3.17E-41 ID:SGTC_252|Compound:miconazole|FD-Score:6.14|P-value:2.10E-10 ID:SGTC_254|Compound:nsc-64875|FD-Score:3.57|P-value:1.24E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:5.41|P-value:1.77E-8 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:3.67|P-value:8.26E-5 ID:SGTC_435|Compound:c-PAF|FD-Score:-3.26|P-value:6.53E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:6.08|P-value:3.02E-10 ID:SGTC_447|Compound:ml7|FD-Score:3.48|P-value:1.75E-4 ID:SGTC_449|Compound:xestospongin c|FD-Score:4.67|P-value:9.10E-7 ID:SGTC_459|Compound:nigericin|FD-Score:3.68|P-value:7.99E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:5.58|P-value:6.39E-9 ID:SGTC_540|Compound:1187-0821|FD-Score:-4.49|P-value:4.09E-6 ID:SGTC_649|Compound:1123-0311|FD-Score:3.66|P-value:8.70E-5 ID:SGTC_654|Compound:1124-0271|FD-Score:3.11|P-value:6.82E-4 ID:SGTC_689|Compound:0833-0039|FD-Score:4.05|P-value:1.66E-5 ID:SGTC_788|Compound:1182-0090|FD-Score:5.44|P-value:1.46E-8 ID:SGTC_814|Compound:3342-0569|FD-Score:3.17|P-value:5.64E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:4.00|P-value:2.11E-5 ID:SGTC_995|Compound:3931-2021|FD-Score:3.24|P-value:4.38E-4 ID:SGTC_997|Compound:3996-0078|FD-Score:3.81|P-value:4.82E-5 ID:SGTC_1165|Compound:k072-0275|FD-Score:3.58|P-value:1.21E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:3.40|P-value:2.38E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:3.38|P-value:2.63E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:4.05|P-value:1.72E-5 ID:SGTC_1412|Compound:3909-8563|FD-Score:4.15|P-value:1.10E-5 ID:SGTC_1502|Compound:4534-4282|FD-Score:3.21|P-value:4.87E-4 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:3.41|P-value:2.31E-4 ID:SGTC_1676|Compound:st018489|FD-Score:4.11|P-value:1.30E-5 ID:SGTC_1679|Compound:4130-1276|FD-Score:4.04|P-value:1.76E-5 ID:SGTC_1735|Compound:st034310|FD-Score:3.51|P-value:1.58E-4 ID:SGTC_1748|Compound:st037455|FD-Score:-4.44|P-value:5.18E-6 ID:SGTC_1750|Compound:st081859|FD-Score:32.10|P-value:4.14E-230 ID:SGTC_1784|Compound:st039334|FD-Score:3.48|P-value:1.79E-4 ID:SGTC_1894|Compound:3',6'-dihydroxyflavone|FD-Score:-3.56|P-value:2.18E-4 ID:SGTC_1901|Compound:st060216|FD-Score:-3.19|P-value:8.37E-4 ID:SGTC_1911|Compound:st060208|FD-Score:3.16|P-value:5.84E-4 ID:SGTC_1953|Compound:st076800|FD-Score:3.26|P-value:4.03E-4 ID:SGTC_1969|Compound:st069479|FD-Score:-3.56|P-value:2.18E-4 ID:SGTC_1972|Compound:st070201|FD-Score:-4.32|P-value:8.99E-6 ID:SGTC_2006|Compound:4011487|FD-Score:3.21|P-value:4.88E-4 ID:SGTC_2120|Compound:5538279|FD-Score:3.14|P-value:6.22E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.66|P-value:9.55E-7 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.33|P-value:3.12E-4 ID:SGTC_2394|Compound:5221648|FD-Score:3.83|P-value:4.37E-5 ID:SGTC_2484|Compound:5809818|FD-Score:3.25|P-value:4.23E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.60|P-value:1.28E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:7.13|P-value:2.19E-13 ID:SGTC_2584|Compound:androsterone|FD-Score:4.32|P-value:4.97E-6 ID:SGTC_2619|Compound:obliquin|FD-Score:6.00|P-value:5.00E-10 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.56|P-value:1.55E-6 ID:SGTC_2707|Compound:st078556|FD-Score:-3.15|P-value:9.52E-4 ID:SGTC_2837|Compound:9008476|FD-Score:-3.54|P-value:2.29E-4 ID:SGTC_2840|Compound:9008771|FD-Score:-3.14|P-value:9.71E-4 ID:SGTC_2865|Compound:9030313|FD-Score:3.08|P-value:7.69E-4 ID:SGTC_2940|Compound:9060638|FD-Score:4.57|P-value:1.52E-6 ID:SGTC_2951|Compound:9072950|FD-Score:3.03|P-value:9.13E-4 ID:SGTC_2991|Compound:9074669|FD-Score:3.01|P-value:9.82E-4 ID:SGTC_3037|Compound:9091026|FD-Score:4.77|P-value:5.66E-7 ID:SGTC_3156|Compound:9099931|FD-Score:3.28|P-value:3.73E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-3.18|P-value:8.47E-4 ID:SGTC_3311|Compound:9131146|FD-Score:3.37|P-value:2.68E-4

Top fitness defect scores for YPR029C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1750 st081859 32.10 2.4 µM 4.14E-230 endomembrane recycling 4 6.33E-226
2 SGTC_221 trifluoperazine 13.20 10.3 µM 3.17E-41 NEO1-PIK1 31 2.76E-40
3 SGTC_207 4185-0010 11.60 4.5 µM 2.43E-32 endomembrane recycling 14 1.43E-31
4 SGTC_2582 agaric acid 7.13 100.0 µM 2.19E-13 redox potentiating 122 5.18E-13
5 SGTC_252 miconazole 6.14 1.3 nM 2.10E-10 ergosterol depletion effects on membrane 12 4.23E-10
6 SGTC_445 e6 berbamine 6.08 66.0 µM 3.02E-10 NEO1-PIK1 29 6.01E-10
7 SGTC_2619 obliquin 6.00 87.9 µM 5.00E-10 cell wall 75 9.86E-10
8 SGTC_514 4466-0038 5.58 2.6 nM 6.39E-9 cell wall 90 1.18E-8
9 SGTC_788 1182-0090 5.44 188.0 µM 1.46E-8 endomembrane recycling 44 2.65E-8
10 SGTC_312 4092-0653 5.41 6.8 µM 1.77E-8 ergosterol depletion effects on membrane 46 3.20E-8
11 SGTC_3037 9091026 4.77 49.5 µM 5.66E-7 NEO1-PIK1 84 9.34E-7
12 SGTC_449 xestospongin c 4.67 11.2 µM 9.10E-7 NEO1-PIK1 42 1.48E-6
13 SGTC_2249 haloperidol 4.66 116.4 µM 9.55E-7 ergosterol depletion effects on membrane 77 1.55E-6
14 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 4.60 3.7 µM 1.28E-6 42 2.07E-6
15 SGTC_2940 9060638 4.57 45.9 µM 1.52E-6 amide catabolism 9 2.44E-6
16 SGTC_2688 kanamycin b 4.56 96.7 µM 1.55E-6 calcium & mitochondrial duress 286 2.50E-6
17 SGTC_2584 androsterone 4.32 48.7 µM 4.97E-6 cell wall 104 7.74E-6
18 SGTC_1412 3909-8563 4.15 178.0 µM 1.10E-5 5 1.68E-5
19 SGTC_1676 st018489 4.11 23.4 µM 1.30E-5 60S ribosome export 29 1.97E-5
20 SGTC_689 0833-0039 4.05 98.0 µM 1.66E-5 ergosterol depletion effects on membrane 34 2.51E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.613 0 YLR169W_d YLR169W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.554 2.02E-269 YLR170C APS1 hom Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
0.528 1.18E-240 YHL019C APM2 hom Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
0.528 1.55E-240 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.436 6.52E-156 YKL135C APL2 hom Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress
0.435 3.61E-155 YPR153W YPR153W hom Putative protein of unknown function
0.435 7.65E-155 YKL136W_d YKL136W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C
0.422 4.83E-145 YBL010C_p YBL010C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
0.410 3.60E-136 YLR171W_d YLR171W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.408 8.12E-135 YFL034W_p YFL034W_p hom Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
0.388 5.66E-121 YGR202C PCT1 hom Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity
0.356 7.96E-101 YCL046W_d YCL046W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C
0.333 6.63E-88 YML072C TCB3 hom Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.327 3.86E-84 YKL098W MTC2 hom Protein of unknown function; mtc2 is synthetically sick with cdc13-1
0.322 1.01E-81 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene