YPR031W / NTO1

Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3

Zygosity: Homozygous strain
fixedexpanded
Profile for YPR031W / NTO1

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:3.61|P-value:3.98E-4 ID:SGTC_52|Compound:0329-0193|FD-Score:5.27|P-value:4.31E-7 ID:SGTC_67|Compound:0335-0847|FD-Score:3.78|P-value:2.19E-4 ID:SGTC_256|Compound:clotrimazole|FD-Score:4.33|P-value:2.76E-5 ID:SGTC_522|Compound:1273-0010|FD-Score:-3.47|P-value:4.59E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:-3.77|P-value:1.59E-4 ID:SGTC_663|Compound:1486-0733|FD-Score:8.58|P-value:4.56E-16 ID:SGTC_885|Compound:0865-0106|FD-Score:4.92|P-value:2.24E-6 ID:SGTC_888|Compound:0916-0002|FD-Score:-3.63|P-value:2.69E-4 ID:SGTC_889|Compound:0919-1524|FD-Score:-3.47|P-value:4.54E-4 ID:SGTC_894|Compound:0960-0238|FD-Score:4.08|P-value:7.38E-5 ID:SGTC_1118|Compound:chelidonine|FD-Score:3.74|P-value:2.49E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:4.84|P-value:3.23E-6 ID:SGTC_1137|Compound:ixabepilone|FD-Score:5.44|P-value:1.91E-7 ID:SGTC_1149|Compound:3909-7961|FD-Score:3.36|P-value:9.16E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:5.11|P-value:9.48E-7 ID:SGTC_1707|Compound:st032323|FD-Score:3.61|P-value:3.91E-4 ID:SGTC_1871|Compound:st057448|FD-Score:-3.46|P-value:4.72E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.78|P-value:2.16E-4 ID:SGTC_2333|Compound:9017922|FD-Score:4.07|P-value:7.49E-5 ID:SGTC_2440|Compound:5628896|FD-Score:3.79|P-value:2.09E-4 ID:SGTC_2508|Compound:celastrol|FD-Score:3.53|P-value:5.19E-4 ID:SGTC_2515|Compound:chrysin|FD-Score:-4.02|P-value:6.48E-5 ID:SGTC_2522|Compound:biochanin a|FD-Score:-3.71|P-value:1.99E-4 ID:SGTC_2528|Compound:xanthyletin|FD-Score:4.96|P-value:1.89E-6 ID:SGTC_2534|Compound:helenine|FD-Score:4.06|P-value:7.91E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:4.68|P-value:6.48E-6 ID:SGTC_2882|Compound:9058044|FD-Score:3.35|P-value:9.19E-4 ID:SGTC_2885|Compound:9057732|FD-Score:3.60|P-value:4.06E-4 ID:SGTC_2929|Compound:9013348|FD-Score:3.37|P-value:8.67E-4 ID:SGTC_3087|Compound:9117406|FD-Score:4.32|P-value:2.86E-5 ID:SGTC_3231|Compound:9132313|FD-Score:-3.85|P-value:1.20E-4 ID:SGTC_3258|Compound:9138477|FD-Score:4.36|P-value:2.48E-5 ID:SGTC_36|Compound:0335-0881|FD-Score:3.61|P-value:3.98E-4 ID:SGTC_52|Compound:0329-0193|FD-Score:5.27|P-value:4.31E-7 ID:SGTC_67|Compound:0335-0847|FD-Score:3.78|P-value:2.19E-4 ID:SGTC_256|Compound:clotrimazole|FD-Score:4.33|P-value:2.76E-5 ID:SGTC_522|Compound:1273-0010|FD-Score:-3.47|P-value:4.59E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:-3.77|P-value:1.59E-4 ID:SGTC_663|Compound:1486-0733|FD-Score:8.58|P-value:4.56E-16 ID:SGTC_885|Compound:0865-0106|FD-Score:4.92|P-value:2.24E-6 ID:SGTC_888|Compound:0916-0002|FD-Score:-3.63|P-value:2.69E-4 ID:SGTC_889|Compound:0919-1524|FD-Score:-3.47|P-value:4.54E-4 ID:SGTC_894|Compound:0960-0238|FD-Score:4.08|P-value:7.38E-5 ID:SGTC_1118|Compound:chelidonine|FD-Score:3.74|P-value:2.49E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:4.84|P-value:3.23E-6 ID:SGTC_1137|Compound:ixabepilone|FD-Score:5.44|P-value:1.91E-7 ID:SGTC_1149|Compound:3909-7961|FD-Score:3.36|P-value:9.16E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:5.11|P-value:9.48E-7 ID:SGTC_1707|Compound:st032323|FD-Score:3.61|P-value:3.91E-4 ID:SGTC_1871|Compound:st057448|FD-Score:-3.46|P-value:4.72E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.78|P-value:2.16E-4 ID:SGTC_2333|Compound:9017922|FD-Score:4.07|P-value:7.49E-5 ID:SGTC_2440|Compound:5628896|FD-Score:3.79|P-value:2.09E-4 ID:SGTC_2508|Compound:celastrol|FD-Score:3.53|P-value:5.19E-4 ID:SGTC_2515|Compound:chrysin|FD-Score:-4.02|P-value:6.48E-5 ID:SGTC_2522|Compound:biochanin a|FD-Score:-3.71|P-value:1.99E-4 ID:SGTC_2528|Compound:xanthyletin|FD-Score:4.96|P-value:1.89E-6 ID:SGTC_2534|Compound:helenine|FD-Score:4.06|P-value:7.91E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:4.68|P-value:6.48E-6 ID:SGTC_2882|Compound:9058044|FD-Score:3.35|P-value:9.19E-4 ID:SGTC_2885|Compound:9057732|FD-Score:3.60|P-value:4.06E-4 ID:SGTC_2929|Compound:9013348|FD-Score:3.37|P-value:8.67E-4 ID:SGTC_3087|Compound:9117406|FD-Score:4.32|P-value:2.86E-5 ID:SGTC_3231|Compound:9132313|FD-Score:-3.85|P-value:1.20E-4 ID:SGTC_3258|Compound:9138477|FD-Score:4.36|P-value:2.48E-5

Top fitness defect scores for YPR031W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_663 1486-0733 8.58 13.9 µM 4.56E-16 cell wall signaling 15 4.69E-18
2 SGTC_1137 ixabepilone 5.44 100.0 µM 1.91E-7 7 2.68E-8
3 SGTC_52 0329-0193 5.27 875.0 nM 4.31E-7 TSC3-RPN4 14 6.76E-8
4 SGTC_1245 0418-0076 5.11 17.2 µM 9.48E-7 ERG2 10 1.65E-7
5 SGTC_2528 xanthyletin 4.96 44.8 µM 1.89E-6 11 3.61E-7
6 SGTC_885 0865-0106 4.92 16.8 µM 2.24E-6 TSC3-RPN4 16 4.36E-7
7 SGTC_1121 fusaric acid 4.84 630.5 nM 3.23E-6 18 6.61E-7
8 SGTC_2778 tioconazole 4.68 65.1 nM 6.48E-6 ergosterol depletion effects on membrane 42 1.45E-6
9 SGTC_3258 9138477 4.36 49.5 µM 2.48E-5 3 6.65E-6
10 SGTC_256 clotrimazole 4.33 625.0 nM 2.76E-5 ergosterol depletion effects on membrane 20 7.50E-6
11 SGTC_3087 9117406 4.32 49.5 µM 2.86E-5 12 7.82E-6
12 SGTC_894 0960-0238 4.08 55.9 µM 7.38E-5 8 2.29E-5
13 SGTC_2333 9017922 4.07 200.0 µM 7.49E-5 22 2.32E-5
14 SGTC_2534 helenine 4.06 20.1 µM 7.91E-5 RSC complex & mRNA processing 14 2.47E-5
15 SGTC_2440 5628896 3.79 157.8 µM 2.09E-4 30 7.41E-5
16 SGTC_1951 sophoraflavanone G 3.78 9.2 µM 2.16E-4 RNA processing & uracil transport 27 7.71E-5
17 SGTC_67 0335-0847 3.78 266.0 µM 2.19E-4 ubiquinone biosynthesis & proteasome 128 7.84E-5
18 SGTC_1118 chelidonine 3.74 707.5 nM 2.49E-4 21 9.04E-5
19 SGTC_1707 st032323 3.61 6.3 µM 3.91E-4 DNA damage response 72 1.51E-4
20 SGTC_36 0335-0881 3.61 14.2 µM 3.98E-4 ubiquinone biosynthesis & proteasome 141 1.54E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.142 1.22E-16 YDR181C SAS4 hom Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p
0.124 4.81E-13 YMR200W ROT1 het Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation
0.118 7.64E-12 YAL053W FLC2 hom Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
0.112 6.92E-11 YGR226C_d YGR226C_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W
0.112 7.35E-11 YMR127C SAS2 hom Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family
0.110 1.46E-10 YOL103W ITR2 hom Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively
0.110 1.65E-10 YPL153C RAD53 het Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication
0.110 1.90E-10 YDR315C IPK1 hom Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable
0.107 5.40E-10 YKL222C YKL222C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine
0.106 6.43E-10 YBL064C PRX1 hom Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress
0.105 9.52E-10 YKL221W MCH2 hom Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport
0.103 2.20E-9 YNL089C_d YNL089C_d hom Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins
0.099 7.93E-9 YGR196C FYV8 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.099 9.09E-9 YOR213C SAS5 hom Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity
0.096 2.38E-8 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria