HIPHOP chemogenomics database
YPR033C / HTS1 
Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome
Zygosity: Heterozygous strain
fixedexpanded
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Top fitness defect scores for YPR033C deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Cofit Genes
Download Cofitness data (tab-delimited text) (excel)| Correlation | pval | ORF | Gene | Zygosity | Description |
|---|---|---|---|---|---|
| 0.241 | 1.67E-45 | YDR540C | IRC4 | hom | Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus |
| 0.238 | 2.28E-44 | YDR351W | SBE2 | hom | Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth |
| 0.221 | 1.91E-38 | YLR150W | STM1 | hom | Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery |
| 0.216 | 8.88E-37 | YNL271C | BNI1 | hom | Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 |
| 0.214 | 4.36E-36 | YLL043W | FPS1 | hom | Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress |
| 0.205 | 5.20E-33 | YBL031W | SHE1 | hom | Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function |
| 0.201 | 7.98E-32 | YDR016C | DAD1 | het | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis |
| 0.200 | 1.04E-31 | YLL021W | SPA2 | hom | Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate |
| 0.184 | 7.86E-27 | YJR111C_p | YJR111C_p | hom | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria |
| 0.183 | 1.07E-26 | YJL080C | SCP160 | hom | Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins |
| 0.182 | 2.20E-26 | YJL073W | JEM1 | hom | DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 |
| 0.176 | 1.14E-24 | YHR129C | ARP1 | hom | Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin |
| 0.172 | 8.44E-24 | YBR091C | TIM12 | het | Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins |
| 0.172 | 9.73E-24 | YPL063W | TIM50 | het | Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel |
| 0.169 | 7.50E-23 | YDL203C | ACK1 | hom | Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria |