YPR033C / HTS1

Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPR033C / HTS1

Click on Significant Values for Screen Details ID:SGTC_138|Compound:0850-0259|FD-Score:8.75|P-value:4.54E-23 ID:SGTC_408|Compound:cantharidin|FD-Score:3.14|P-value:1.78E-4 ID:SGTC_438|Compound:sphingosine|FD-Score:-3.19|P-value:2.43E-4 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:2.83|P-value:6.25E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:-2.98|P-value:5.87E-4 ID:SGTC_648|Compound:0375-0473|FD-Score:3.04|P-value:2.74E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:3.13|P-value:1.85E-4 ID:SGTC_817|Compound:1391-0715|FD-Score:3.79|P-value:8.69E-6 ID:SGTC_945|Compound:3381-0768|FD-Score:2.84|P-value:6.04E-4 ID:SGTC_980|Compound:1473-0395|FD-Score:-3.09|P-value:3.71E-4 ID:SGTC_1079|Compound:idarubicin|FD-Score:2.81|P-value:6.87E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.85|P-value:1.24E-5 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.58|P-value:2.48E-5 ID:SGTC_1086|Compound:cerivastatin|FD-Score:2.87|P-value:5.46E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:-4.60|P-value:2.18E-7 ID:SGTC_1254|Compound:0550-0035|FD-Score:2.99|P-value:3.36E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:6.09|P-value:3.63E-12 ID:SGTC_1263|Compound:0774-1208|FD-Score:2.80|P-value:7.17E-4 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.61|P-value:2.10E-5 ID:SGTC_1365|Compound:1672-3266|FD-Score:2.81|P-value:6.84E-4 ID:SGTC_1387|Compound:3342-1166|FD-Score:-2.89|P-value:8.20E-4 ID:SGTC_1453|Compound:4237-0541|FD-Score:3.05|P-value:2.58E-4 ID:SGTC_1535|Compound:tetrahydropapaveroline hydrobromide|FD-Score:2.80|P-value:6.98E-4 ID:SGTC_1694|Compound:st024011|FD-Score:4.50|P-value:1.80E-7 ID:SGTC_1710|Compound:st031758|FD-Score:3.32|P-value:8.15E-5 ID:SGTC_1754|Compound:st042065|FD-Score:3.72|P-value:1.22E-5 ID:SGTC_1803|Compound:st050068|FD-Score:2.83|P-value:6.22E-4 ID:SGTC_2039|Compound:Aminomethylbenzodioxan|FD-Score:-2.93|P-value:7.04E-4 ID:SGTC_2246|Compound:7293507|FD-Score:3.38|P-value:6.26E-5 ID:SGTC_2248|Compound:tofa|FD-Score:2.84|P-value:5.94E-4 ID:SGTC_2292|Compound:7671323|FD-Score:4.58|P-value:1.13E-7 ID:SGTC_2396|Compound:5342950|FD-Score:3.12|P-value:1.90E-4 ID:SGTC_2476|Compound:5806916|FD-Score:2.74|P-value:9.03E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:2.85|P-value:5.91E-4 ID:SGTC_2794|Compound:7785382|FD-Score:2.99|P-value:3.32E-4 ID:SGTC_3006|Compound:9075424|FD-Score:-3.68|P-value:2.85E-5 ID:SGTC_3036|Compound:9090181|FD-Score:-2.85|P-value:9.43E-4 ID:SGTC_3081|Compound:9117243|FD-Score:3.20|P-value:1.40E-4 ID:SGTC_3314|Compound:9135860|FD-Score:3.53|P-value:3.17E-5 ID:SGTC_3327|Compound:9140648|FD-Score:2.83|P-value:6.32E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:8.75|P-value:4.54E-23 ID:SGTC_408|Compound:cantharidin|FD-Score:3.14|P-value:1.78E-4 ID:SGTC_438|Compound:sphingosine|FD-Score:-3.19|P-value:2.43E-4 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:2.83|P-value:6.25E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:-2.98|P-value:5.87E-4 ID:SGTC_648|Compound:0375-0473|FD-Score:3.04|P-value:2.74E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:3.13|P-value:1.85E-4 ID:SGTC_817|Compound:1391-0715|FD-Score:3.79|P-value:8.69E-6 ID:SGTC_945|Compound:3381-0768|FD-Score:2.84|P-value:6.04E-4 ID:SGTC_980|Compound:1473-0395|FD-Score:-3.09|P-value:3.71E-4 ID:SGTC_1079|Compound:idarubicin|FD-Score:2.81|P-value:6.87E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.85|P-value:1.24E-5 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.58|P-value:2.48E-5 ID:SGTC_1086|Compound:cerivastatin|FD-Score:2.87|P-value:5.46E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:-4.60|P-value:2.18E-7 ID:SGTC_1254|Compound:0550-0035|FD-Score:2.99|P-value:3.36E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:6.09|P-value:3.63E-12 ID:SGTC_1263|Compound:0774-1208|FD-Score:2.80|P-value:7.17E-4 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.61|P-value:2.10E-5 ID:SGTC_1365|Compound:1672-3266|FD-Score:2.81|P-value:6.84E-4 ID:SGTC_1387|Compound:3342-1166|FD-Score:-2.89|P-value:8.20E-4 ID:SGTC_1453|Compound:4237-0541|FD-Score:3.05|P-value:2.58E-4 ID:SGTC_1535|Compound:tetrahydropapaveroline hydrobromide|FD-Score:2.80|P-value:6.98E-4 ID:SGTC_1694|Compound:st024011|FD-Score:4.50|P-value:1.80E-7 ID:SGTC_1710|Compound:st031758|FD-Score:3.32|P-value:8.15E-5 ID:SGTC_1754|Compound:st042065|FD-Score:3.72|P-value:1.22E-5 ID:SGTC_1803|Compound:st050068|FD-Score:2.83|P-value:6.22E-4 ID:SGTC_2039|Compound:Aminomethylbenzodioxan|FD-Score:-2.93|P-value:7.04E-4 ID:SGTC_2246|Compound:7293507|FD-Score:3.38|P-value:6.26E-5 ID:SGTC_2248|Compound:tofa|FD-Score:2.84|P-value:5.94E-4 ID:SGTC_2292|Compound:7671323|FD-Score:4.58|P-value:1.13E-7 ID:SGTC_2396|Compound:5342950|FD-Score:3.12|P-value:1.90E-4 ID:SGTC_2476|Compound:5806916|FD-Score:2.74|P-value:9.03E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:2.85|P-value:5.91E-4 ID:SGTC_2794|Compound:7785382|FD-Score:2.99|P-value:3.32E-4 ID:SGTC_3006|Compound:9075424|FD-Score:-3.68|P-value:2.85E-5 ID:SGTC_3036|Compound:9090181|FD-Score:-2.85|P-value:9.43E-4 ID:SGTC_3081|Compound:9117243|FD-Score:3.20|P-value:1.40E-4 ID:SGTC_3314|Compound:9135860|FD-Score:3.53|P-value:3.17E-5 ID:SGTC_3327|Compound:9140648|FD-Score:2.83|P-value:6.32E-4

Top fitness defect scores for YPR033C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_138 0850-0259 8.75 40.9 µM 4.54E-23 cell wall 15 1.07E-18 0.02
2 SGTC_1255 0566-0045 6.09 221.0 µM 3.63E-12 excess fatty acid 6 5.82E-10 0.56
3 SGTC_2292 7671323 4.58 168.1 µM 1.13E-7 RNA processing & uracil transport 9 2.32E-6 0.03
4 SGTC_1694 st024011 4.50 44.9 µM 1.80E-7 ERG2 5 3.35E-6 0.43
5 SGTC_817 1391-0715 3.79 46.0 µM 8.69E-6 10 7.50E-5 0.19
6 SGTC_1754 st042065 3.72 16.1 µM 1.22E-5 NEO1-PIK1 37 9.86E-5 0.14
7 SGTC_1348 1493-0373 3.61 93.8 µM 2.10E-5 6 1.52E-4 0.01
8 SGTC_1083 didemethylchlorpromazine sulfoxide 3.58 707.4 nM 2.48E-5 redox potentiating 29 1.74E-4 0.15
9 SGTC_3314 9135860 3.53 59.4 µM 3.17E-5 5 2.12E-4 0.35
10 SGTC_2246 7293507 3.38 91.6 µM 6.26E-5 19 3.65E-4 0.10
11 SGTC_1710 st031758 3.32 61.5 µM 8.15E-5 7 4.51E-4 0.05
12 SGTC_3081 9117243 3.20 49.5 µM 1.40E-4 fatty acid desaturase (OLE1) 43 6.98E-4 0.00
13 SGTC_408 cantharidin 3.14 100.0 µM 1.78E-4 cell wall signaling 17 8.45E-4 0.03
14 SGTC_673 1082-0474 3.13 110.0 µM 1.85E-4 RSC complex & mRNA processing 69 8.70E-4 0.01
15 SGTC_2396 5342950 3.12 42.8 µM 1.90E-4 RSC complex & mRNA processing 53 8.90E-4 0.04
16 SGTC_1453 4237-0541 3.05 195.0 µM 2.58E-4 heme biosynthesis & mitochondrial translocase 28 0.00114 0.05
17 SGTC_648 0375-0473 3.04 33.3 µM 2.74E-4 13 0.00119 0.02
18 SGTC_2794 7785382 2.99 27.3 µM 3.32E-4 28 0.00139 0.06
19 SGTC_1254 0550-0035 2.99 264.0 µM 3.36E-4 18 0.00141 0.04
20 SGTC_1086 cerivastatin 2.87 2.2 µM 5.46E-4 azole & statin 10 0.00208 0.08

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.241 1.67E-45 YDR540C IRC4 hom Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
0.238 2.28E-44 YDR351W SBE2 hom Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth
0.221 1.91E-38 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.216 8.88E-37 YNL271C BNI1 hom Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1
0.214 4.36E-36 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.205 5.20E-33 YBL031W SHE1 hom Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function
0.201 7.98E-32 YDR016C DAD1 het Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
0.200 1.04E-31 YLL021W SPA2 hom Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate
0.184 7.86E-27 YJR111C_p YJR111C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria
0.183 1.07E-26 YJL080C SCP160 hom Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
0.182 2.20E-26 YJL073W JEM1 hom DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2
0.176 1.14E-24 YHR129C ARP1 hom Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin
0.172 8.44E-24 YBR091C TIM12 het Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
0.172 9.73E-24 YPL063W TIM50 het Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel
0.169 7.50E-23 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria