YPR035W / GLN1

Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPR035W / GLN1

Click on Significant Values for Screen Details ID:SGTC_48|Compound:0382-0018|FD-Score:-3.51|P-value:5.36E-5 ID:SGTC_49|Compound:0717-0965|FD-Score:-3.59|P-value:3.82E-5 ID:SGTC_50|Compound:0929-0078|FD-Score:-3.63|P-value:3.21E-5 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:2.86|P-value:8.78E-4 ID:SGTC_324|Compound:1315-0088|FD-Score:3.45|P-value:7.87E-5 ID:SGTC_375|Compound:3970-0961|FD-Score:7.62|P-value:3.17E-17 ID:SGTC_382|Compound:3229-0966|FD-Score:4.00|P-value:6.02E-6 ID:SGTC_393|Compound:acivicin|FD-Score:17.80|P-value:5.27E-85 ID:SGTC_428|Compound:3448-4153|FD-Score:-2.97|P-value:5.24E-4 ID:SGTC_432|Compound:NS 1619|FD-Score:-3.62|P-value:3.36E-5 ID:SGTC_448|Compound:monastrol|FD-Score:3.07|P-value:3.90E-4 ID:SGTC_517|Compound:1365-0151|FD-Score:-3.23|P-value:1.88E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:-3.54|P-value:4.67E-5 ID:SGTC_525|Compound:k781-2675|FD-Score:5.69|P-value:2.20E-10 ID:SGTC_528|Compound:3492-0019|FD-Score:4.52|P-value:3.77E-7 ID:SGTC_531|Compound:3788-1495|FD-Score:6.12|P-value:9.46E-12 ID:SGTC_612|Compound:0302-0167|FD-Score:2.86|P-value:8.64E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:2.98|P-value:5.63E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:3.16|P-value:2.74E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:3.21|P-value:2.22E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:-3.21|P-value:2.02E-4 ID:SGTC_931|Compound:2812-0076|FD-Score:3.23|P-value:2.02E-4 ID:SGTC_959|Compound:1222-0044|FD-Score:-3.69|P-value:2.36E-5 ID:SGTC_1031|Compound:k035-0009|FD-Score:-3.15|P-value:2.55E-4 ID:SGTC_1038|Compound:k292-0204|FD-Score:-3.05|P-value:3.86E-4 ID:SGTC_1171|Compound:0368-0077|FD-Score:-2.83|P-value:8.87E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:4.29|P-value:1.29E-6 ID:SGTC_1248|Compound:0469-0687|FD-Score:4.30|P-value:1.21E-6 ID:SGTC_1340|Compound:1486-1359|FD-Score:-2.84|P-value:8.54E-4 ID:SGTC_1463|Compound:k081-0032|FD-Score:-3.41|P-value:8.41E-5 ID:SGTC_1486|Compound:3970-0793|FD-Score:2.98|P-value:5.47E-4 ID:SGTC_1496|Compound:4469-0713|FD-Score:2.97|P-value:5.73E-4 ID:SGTC_1637|Compound:st007924|FD-Score:-4.51|P-value:3.38E-7 ID:SGTC_1771|Compound:st048882|FD-Score:-2.95|P-value:5.79E-4 ID:SGTC_1841|Compound:st054148|FD-Score:4.66|P-value:1.61E-7 ID:SGTC_1963|Compound:st076565|FD-Score:3.50|P-value:6.47E-5 ID:SGTC_2010|Compound:4012422|FD-Score:-3.78|P-value:1.55E-5 ID:SGTC_2124|Compound:5246320|FD-Score:3.25|P-value:1.86E-4 ID:SGTC_2311|Compound:7694451|FD-Score:-3.24|P-value:1.74E-4 ID:SGTC_2347|Compound:9049783|FD-Score:-3.52|P-value:5.32E-5 ID:SGTC_2386|Compound:9075165|FD-Score:2.83|P-value:9.74E-4 ID:SGTC_2437|Compound:5741531|FD-Score:2.93|P-value:6.69E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:3.24|P-value:1.96E-4 ID:SGTC_2516|Compound:gossypol|FD-Score:3.46|P-value:7.75E-5 ID:SGTC_2663|Compound:amphotericin b|FD-Score:3.09|P-value:3.60E-4 ID:SGTC_2782|Compound:7736369|FD-Score:-3.45|P-value:7.05E-5 ID:SGTC_2783|Compound:7738165|FD-Score:-4.47|P-value:4.34E-7 ID:SGTC_2784|Compound:7567673|FD-Score:-4.46|P-value:4.55E-7 ID:SGTC_2820|Compound:7989630|FD-Score:-3.37|P-value:1.04E-4 ID:SGTC_2822|Compound:7994718|FD-Score:5.24|P-value:4.71E-9 ID:SGTC_2945|Compound:9067681|FD-Score:3.61|P-value:3.85E-5 ID:SGTC_2950|Compound:9072895|FD-Score:-4.16|P-value:2.26E-6 ID:SGTC_2951|Compound:9072950|FD-Score:-5.48|P-value:7.91E-10 ID:SGTC_3240|Compound:9133527|FD-Score:-3.44|P-value:7.42E-5 ID:SGTC_3251|Compound:9135047|FD-Score:2.88|P-value:8.11E-4 ID:SGTC_48|Compound:0382-0018|FD-Score:-3.51|P-value:5.36E-5 ID:SGTC_49|Compound:0717-0965|FD-Score:-3.59|P-value:3.82E-5 ID:SGTC_50|Compound:0929-0078|FD-Score:-3.63|P-value:3.21E-5 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:2.86|P-value:8.78E-4 ID:SGTC_324|Compound:1315-0088|FD-Score:3.45|P-value:7.87E-5 ID:SGTC_375|Compound:3970-0961|FD-Score:7.62|P-value:3.17E-17 ID:SGTC_382|Compound:3229-0966|FD-Score:4.00|P-value:6.02E-6 ID:SGTC_393|Compound:acivicin|FD-Score:17.80|P-value:5.27E-85 ID:SGTC_428|Compound:3448-4153|FD-Score:-2.97|P-value:5.24E-4 ID:SGTC_432|Compound:NS 1619|FD-Score:-3.62|P-value:3.36E-5 ID:SGTC_448|Compound:monastrol|FD-Score:3.07|P-value:3.90E-4 ID:SGTC_517|Compound:1365-0151|FD-Score:-3.23|P-value:1.88E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:-3.54|P-value:4.67E-5 ID:SGTC_525|Compound:k781-2675|FD-Score:5.69|P-value:2.20E-10 ID:SGTC_528|Compound:3492-0019|FD-Score:4.52|P-value:3.77E-7 ID:SGTC_531|Compound:3788-1495|FD-Score:6.12|P-value:9.46E-12 ID:SGTC_612|Compound:0302-0167|FD-Score:2.86|P-value:8.64E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:2.98|P-value:5.63E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:3.16|P-value:2.74E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:3.21|P-value:2.22E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:-3.21|P-value:2.02E-4 ID:SGTC_931|Compound:2812-0076|FD-Score:3.23|P-value:2.02E-4 ID:SGTC_959|Compound:1222-0044|FD-Score:-3.69|P-value:2.36E-5 ID:SGTC_1031|Compound:k035-0009|FD-Score:-3.15|P-value:2.55E-4 ID:SGTC_1038|Compound:k292-0204|FD-Score:-3.05|P-value:3.86E-4 ID:SGTC_1171|Compound:0368-0077|FD-Score:-2.83|P-value:8.87E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:4.29|P-value:1.29E-6 ID:SGTC_1248|Compound:0469-0687|FD-Score:4.30|P-value:1.21E-6 ID:SGTC_1340|Compound:1486-1359|FD-Score:-2.84|P-value:8.54E-4 ID:SGTC_1463|Compound:k081-0032|FD-Score:-3.41|P-value:8.41E-5 ID:SGTC_1486|Compound:3970-0793|FD-Score:2.98|P-value:5.47E-4 ID:SGTC_1496|Compound:4469-0713|FD-Score:2.97|P-value:5.73E-4 ID:SGTC_1637|Compound:st007924|FD-Score:-4.51|P-value:3.38E-7 ID:SGTC_1771|Compound:st048882|FD-Score:-2.95|P-value:5.79E-4 ID:SGTC_1841|Compound:st054148|FD-Score:4.66|P-value:1.61E-7 ID:SGTC_1963|Compound:st076565|FD-Score:3.50|P-value:6.47E-5 ID:SGTC_2010|Compound:4012422|FD-Score:-3.78|P-value:1.55E-5 ID:SGTC_2124|Compound:5246320|FD-Score:3.25|P-value:1.86E-4 ID:SGTC_2311|Compound:7694451|FD-Score:-3.24|P-value:1.74E-4 ID:SGTC_2347|Compound:9049783|FD-Score:-3.52|P-value:5.32E-5 ID:SGTC_2386|Compound:9075165|FD-Score:2.83|P-value:9.74E-4 ID:SGTC_2437|Compound:5741531|FD-Score:2.93|P-value:6.69E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:3.24|P-value:1.96E-4 ID:SGTC_2516|Compound:gossypol|FD-Score:3.46|P-value:7.75E-5 ID:SGTC_2663|Compound:amphotericin b|FD-Score:3.09|P-value:3.60E-4 ID:SGTC_2782|Compound:7736369|FD-Score:-3.45|P-value:7.05E-5 ID:SGTC_2783|Compound:7738165|FD-Score:-4.47|P-value:4.34E-7 ID:SGTC_2784|Compound:7567673|FD-Score:-4.46|P-value:4.55E-7 ID:SGTC_2820|Compound:7989630|FD-Score:-3.37|P-value:1.04E-4 ID:SGTC_2822|Compound:7994718|FD-Score:5.24|P-value:4.71E-9 ID:SGTC_2945|Compound:9067681|FD-Score:3.61|P-value:3.85E-5 ID:SGTC_2950|Compound:9072895|FD-Score:-4.16|P-value:2.26E-6 ID:SGTC_2951|Compound:9072950|FD-Score:-5.48|P-value:7.91E-10 ID:SGTC_3240|Compound:9133527|FD-Score:-3.44|P-value:7.42E-5 ID:SGTC_3251|Compound:9135047|FD-Score:2.88|P-value:8.11E-4

Top fitness defect scores for YPR035W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_393 acivicin 17.80 6.2 µM 5.27E-85 1 6.46E-71 11.20
2 SGTC_375 3970-0961 7.62 9.3 µM 3.17E-17 tubulin folding & SWR complex 1 1.25E-14 1.52
3 SGTC_531 3788-1495 6.12 174.0 µM 9.46E-12 unfolded protein response 3 4.55E-10 1.11
4 SGTC_525 k781-2675 5.69 26.7 µM 2.20E-10 redox potentiating 25 6.29E-9 0.11
5 SGTC_2822 7994718 5.24 71.4 µM 4.71E-9 3 8.10E-8 0.70
6 SGTC_1841 st054148 4.66 70.0 µM 1.61E-7 2 1.55E-6 0.45
7 SGTC_528 3492-0019 4.52 172.0 µM 3.77E-7 calcium & mitochondrial duress 17 3.16E-6 0.32
8 SGTC_1248 0469-0687 4.30 58.6 µM 1.21E-6 7 8.40E-6 0.11
9 SGTC_1245 0418-0076 4.29 17.2 µM 1.29E-6 ERG2 8 8.86E-6 0.45
10 SGTC_382 3229-0966 4.00 23.2 µM 6.02E-6 5 3.22E-5 0.38
11 SGTC_2945 9067681 3.61 61.8 µM 3.85E-5 6 1.52E-4 0.29
12 SGTC_1963 st076565 3.50 50.3 µM 6.47E-5 7 2.36E-4 0.14
13 SGTC_2516 gossypol 3.46 71.5 µM 7.75E-5 13 2.74E-4 0.08
14 SGTC_324 1315-0088 3.45 19.6 µM 7.87E-5 RSC & ERG11 16 2.78E-4 0.16
15 SGTC_2124 5246320 3.25 200.0 µM 1.86E-4 heme biosynthesis & mitochondrial translocase 39 5.73E-4 0.06
16 SGTC_2514 salinomycin 3.24 42.7 µM 1.96E-4 7 5.98E-4 0.12
17 SGTC_931 2812-0076 3.23 566.0 µM 2.02E-4 15 6.14E-4 0.12
18 SGTC_669 0108-0021 3.21 26.2 µM 2.22E-4 fatty acid desaturase (OLE1) 20 6.64E-4 0.05
19 SGTC_628 1636-0253 3.16 16.0 µM 2.74E-4 sphingolipid biosynthesis & PDR1 20 7.94E-4 0.01
20 SGTC_2663 amphotericin b 3.09 100.0 µM 3.60E-4 18 9.99E-4 0.07

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.293 2.81E-67 YJR103W URA8 hom Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication
0.172 9.49E-24 YPL218W SAR1 het GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport
0.150 2.49E-18 YHL023C NPR3 hom Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair
0.148 7.47E-18 YFR053C HXK1 hom Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication
0.145 2.61E-17 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.131 3.14E-14 YMR308C PSE1 het Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p
0.128 1.01E-13 YPR174C YPR174C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery; potential Cdc28p substrate; binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study; relative distribution to foci at the nuclear periphery increases upon DNA replication stress; YPR174C has a paralog, NBP1, that arose from the whole genome duplication
0.116 1.79E-11 YBR274W CHK1 hom Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase
0.115 2.17E-11 YIR019C FLO11 hom GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain
0.114 3.08E-11 YBL103C RTG3 hom Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways
0.112 6.61E-11 YPR053C_d YPR053C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C
0.111 1.28E-10 YEL062W NPR2 hom Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline
0.103 2.37E-9 YDR073W SNF11 hom Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions
0.102 3.02E-9 YER038W-A_d YER038W-A_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria
0.100 6.33E-9 YLR177W_p YLR177W_p hom Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication