YPR036W / VMA13

Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase

Zygosity: Homozygous strain
fixedexpanded
Profile for YPR036W / VMA13

Click on Significant Values for Screen Details ID:SGTC_71|Compound:0417-1750|FD-Score:5.49|P-value:9.98E-6 ID:SGTC_73|Compound:0327-0325|FD-Score:-4.48|P-value:2.50E-4 ID:SGTC_75|Compound:0159-0059|FD-Score:5.70|P-value:4.78E-6 ID:SGTC_97|Compound:0535-0701|FD-Score:-4.93|P-value:6.49E-5 ID:SGTC_100|Compound:0086-0128|FD-Score:4.95|P-value:6.07E-5 ID:SGTC_157|Compound:1348-1695|FD-Score:4.11|P-value:6.98E-4 ID:SGTC_217|Compound:ketoconazole|FD-Score:-4.76|P-value:1.09E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:5.31|P-value:1.85E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.87|P-value:2.58E-6 ID:SGTC_335|Compound:4077-0256|FD-Score:-4.11|P-value:7.18E-4 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:-4.30|P-value:4.25E-4 ID:SGTC_747|Compound:3448-8087|FD-Score:-4.44|P-value:2.86E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:5.59|P-value:7.07E-6 ID:SGTC_848|Compound:0368-0089|FD-Score:4.45|P-value:2.76E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:5.86|P-value:2.67E-6 ID:SGTC_1054|Compound:3429-0080|FD-Score:4.00|P-value:9.52E-4 ID:SGTC_1124|Compound:fisetin|FD-Score:4.15|P-value:6.29E-4 ID:SGTC_1172|Compound:0370-0043|FD-Score:4.00|P-value:9.38E-4 ID:SGTC_1218|Compound:1348-1416|FD-Score:4.02|P-value:8.97E-4 ID:SGTC_1351|Compound:1496-0003|FD-Score:4.03|P-value:8.81E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:6.08|P-value:1.17E-6 ID:SGTC_1374|Compound:2889-5571|FD-Score:-5.01|P-value:4.96E-5 ID:SGTC_1447|Compound:4239-0816|FD-Score:-4.38|P-value:3.36E-4 ID:SGTC_1458|Compound:k015-0034|FD-Score:5.81|P-value:3.22E-6 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:5.21|P-value:2.60E-5 ID:SGTC_1658|Compound:st012944|FD-Score:5.62|P-value:6.23E-6 ID:SGTC_1660|Compound:st012960|FD-Score:5.20|P-value:2.64E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.60|P-value:1.77E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.98|P-value:9.79E-4 ID:SGTC_1808|Compound:st050063|FD-Score:4.98|P-value:5.54E-5 ID:SGTC_1816|Compound:st051957|FD-Score:4.21|P-value:5.39E-4 ID:SGTC_1909|Compound:st060837|FD-Score:5.62|P-value:6.29E-6 ID:SGTC_1911|Compound:st060208|FD-Score:7.69|P-value:1.18E-9 ID:SGTC_1917|Compound:st066507|FD-Score:4.59|P-value:1.80E-4 ID:SGTC_1919|Compound:st067113|FD-Score:4.97|P-value:5.56E-5 ID:SGTC_2043|Compound:5101342|FD-Score:4.04|P-value:8.52E-4 ID:SGTC_2168|Compound:5727979|FD-Score:5.58|P-value:7.39E-6 ID:SGTC_2173|Compound:5763948|FD-Score:5.79|P-value:3.44E-6 ID:SGTC_2302|Compound:7771753|FD-Score:-4.96|P-value:5.94E-5 ID:SGTC_2309|Compound:7664088|FD-Score:-7.41|P-value:4.34E-9 ID:SGTC_2378|Compound:9074213|FD-Score:4.98|P-value:5.53E-5 ID:SGTC_2481|Compound:5478948|FD-Score:4.73|P-value:1.21E-4 ID:SGTC_2483|Compound:5729050|FD-Score:-6.03|P-value:1.39E-6 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:4.69|P-value:1.33E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:4.23|P-value:5.09E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:5.51|P-value:9.16E-6 ID:SGTC_2661|Compound:dactinomycin|FD-Score:4.96|P-value:5.83E-5 ID:SGTC_2824|Compound:7998404|FD-Score:4.42|P-value:2.99E-4 ID:SGTC_2981|Compound:9013952|FD-Score:4.14|P-value:6.41E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-4.66|P-value:1.47E-4 ID:SGTC_3274|Compound:9138298|FD-Score:4.21|P-value:5.34E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:5.49|P-value:9.98E-6 ID:SGTC_73|Compound:0327-0325|FD-Score:-4.48|P-value:2.50E-4 ID:SGTC_75|Compound:0159-0059|FD-Score:5.70|P-value:4.78E-6 ID:SGTC_97|Compound:0535-0701|FD-Score:-4.93|P-value:6.49E-5 ID:SGTC_100|Compound:0086-0128|FD-Score:4.95|P-value:6.07E-5 ID:SGTC_157|Compound:1348-1695|FD-Score:4.11|P-value:6.98E-4 ID:SGTC_217|Compound:ketoconazole|FD-Score:-4.76|P-value:1.09E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:5.31|P-value:1.85E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.87|P-value:2.58E-6 ID:SGTC_335|Compound:4077-0256|FD-Score:-4.11|P-value:7.18E-4 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:-4.30|P-value:4.25E-4 ID:SGTC_747|Compound:3448-8087|FD-Score:-4.44|P-value:2.86E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:5.59|P-value:7.07E-6 ID:SGTC_848|Compound:0368-0089|FD-Score:4.45|P-value:2.76E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:5.86|P-value:2.67E-6 ID:SGTC_1054|Compound:3429-0080|FD-Score:4.00|P-value:9.52E-4 ID:SGTC_1124|Compound:fisetin|FD-Score:4.15|P-value:6.29E-4 ID:SGTC_1172|Compound:0370-0043|FD-Score:4.00|P-value:9.38E-4 ID:SGTC_1218|Compound:1348-1416|FD-Score:4.02|P-value:8.97E-4 ID:SGTC_1351|Compound:1496-0003|FD-Score:4.03|P-value:8.81E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:6.08|P-value:1.17E-6 ID:SGTC_1374|Compound:2889-5571|FD-Score:-5.01|P-value:4.96E-5 ID:SGTC_1447|Compound:4239-0816|FD-Score:-4.38|P-value:3.36E-4 ID:SGTC_1458|Compound:k015-0034|FD-Score:5.81|P-value:3.22E-6 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:5.21|P-value:2.60E-5 ID:SGTC_1658|Compound:st012944|FD-Score:5.62|P-value:6.23E-6 ID:SGTC_1660|Compound:st012960|FD-Score:5.20|P-value:2.64E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.60|P-value:1.77E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.98|P-value:9.79E-4 ID:SGTC_1808|Compound:st050063|FD-Score:4.98|P-value:5.54E-5 ID:SGTC_1816|Compound:st051957|FD-Score:4.21|P-value:5.39E-4 ID:SGTC_1909|Compound:st060837|FD-Score:5.62|P-value:6.29E-6 ID:SGTC_1911|Compound:st060208|FD-Score:7.69|P-value:1.18E-9 ID:SGTC_1917|Compound:st066507|FD-Score:4.59|P-value:1.80E-4 ID:SGTC_1919|Compound:st067113|FD-Score:4.97|P-value:5.56E-5 ID:SGTC_2043|Compound:5101342|FD-Score:4.04|P-value:8.52E-4 ID:SGTC_2168|Compound:5727979|FD-Score:5.58|P-value:7.39E-6 ID:SGTC_2173|Compound:5763948|FD-Score:5.79|P-value:3.44E-6 ID:SGTC_2302|Compound:7771753|FD-Score:-4.96|P-value:5.94E-5 ID:SGTC_2309|Compound:7664088|FD-Score:-7.41|P-value:4.34E-9 ID:SGTC_2378|Compound:9074213|FD-Score:4.98|P-value:5.53E-5 ID:SGTC_2481|Compound:5478948|FD-Score:4.73|P-value:1.21E-4 ID:SGTC_2483|Compound:5729050|FD-Score:-6.03|P-value:1.39E-6 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:4.69|P-value:1.33E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:4.23|P-value:5.09E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:5.51|P-value:9.16E-6 ID:SGTC_2661|Compound:dactinomycin|FD-Score:4.96|P-value:5.83E-5 ID:SGTC_2824|Compound:7998404|FD-Score:4.42|P-value:2.99E-4 ID:SGTC_2981|Compound:9013952|FD-Score:4.14|P-value:6.41E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-4.66|P-value:1.47E-4 ID:SGTC_3274|Compound:9138298|FD-Score:4.21|P-value:5.34E-4

Top fitness defect scores for YPR036W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1911 st060208 7.69 74.3 µM 1.18E-9 1 7.59E-15
2 SGTC_1368 1852-0141 6.08 284.0 µM 1.17E-6 ERG2 10 6.15E-10
3 SGTC_1051 3253-3903 5.86 78.0 µM 2.67E-6 2 2.35E-9
4 SGTC_1458 k015-0034 5.81 6.3 µM 3.22E-6 5 3.19E-9
5 SGTC_2173 5763948 5.79 167.0 µM 3.44E-6 tubulin folding & SWR complex 4 3.55E-9
6 SGTC_75 0159-0059 5.70 199.0 µM 4.78E-6 fatty acid desaturase (OLE1) 6 6.06E-9
7 SGTC_1658 st012944 5.62 20.0 µM 6.23E-6 iron homeostasis 35 9.34E-9
8 SGTC_1909 st060837 5.62 51.5 µM 6.29E-6 22 9.48E-9
9 SGTC_841 0251-0215 5.59 91.2 µM 7.07E-6 41 1.15E-8
10 SGTC_2168 5727979 5.58 200.0 µM 7.39E-6 5 1.23E-8
11 SGTC_2615 purpurogallin-4-carboxylic acid 5.51 100.0 µM 9.16E-6 redox potentiating 31 1.75E-8
12 SGTC_71 0417-1750 5.49 47.8 µM 9.98E-6 Golgi 19 2.01E-8
13 SGTC_219 mitoxanthrone 5.31 164.4 µM 1.85E-5 DNA intercalators 23 5.49E-8
14 SGTC_1595 6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin 5.21 67.5 µM 2.60E-5 16 9.53E-8
15 SGTC_1660 st012960 5.20 28.1 µM 2.64E-5 iron homeostasis 15 9.81E-8
16 SGTC_2378 9074213 4.98 121.4 µM 5.53E-5 RSC complex & mRNA processing 3 3.25E-7
17 SGTC_1808 st050063 4.98 61.5 µM 5.54E-5 1 3.26E-7
18 SGTC_1919 st067113 4.97 52.3 µM 5.56E-5 TSC3-RPN4 16 3.28E-7
19 SGTC_2661 dactinomycin 4.96 13.0 µM 5.83E-5 redox potentiating 6 3.54E-7
20 SGTC_100 0086-0128 4.95 49.6 µM 6.07E-5 copper-dependent oxidative stress 23 3.78E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.189 3.12E-28 YDL185W VMA1 hom Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease
0.142 1.34E-16 YGR020C VMA7 hom Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane
0.140 4.08E-16 YLR315W NKP2 hom Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2
0.139 7.92E-16 YGL071W AFT1 hom Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.136 2.18E-15 YOR331C_d YOR331C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W
0.130 3.55E-14 YJR040W GEF1 hom Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism
0.130 4.06E-14 YHR026W VMA16 hom Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain
0.129 5.72E-14 YDR203W_d YDR203W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.121 1.86E-12 YEL051W VMA8 hom Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis
0.119 4.45E-12 YGL168W HUR1 hom Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene
0.112 1.07E-10 YMR123W PKR1 hom V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress
0.109 2.13E-10 YDR323C PEP7 hom Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
0.101 4.38E-9 YDR495C VPS3 hom Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
0.101 5.55E-9 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.098 1.45E-8 YGL212W VAM7 hom Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress