YPR041W / TIF5

Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPR041W / TIF5

Click on Significant Values for Screen Details ID:SGTC_68|Compound:0986-0284|FD-Score:5.65|P-value:2.20E-9 ID:SGTC_72|Compound:0744-0168|FD-Score:4.16|P-value:7.69E-6 ID:SGTC_74|Compound:000s-0487|FD-Score:3.07|P-value:7.14E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:3.76|P-value:4.68E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:3.57|P-value:1.04E-4 ID:SGTC_320|Compound:4092-0846|FD-Score:3.58|P-value:9.86E-5 ID:SGTC_363|Compound:0455-0021|FD-Score:-5.54|P-value:4.38E-9 ID:SGTC_369|Compound:0957-0393|FD-Score:5.56|P-value:3.83E-9 ID:SGTC_529|Compound:4076-0302|FD-Score:5.81|P-value:7.95E-10 ID:SGTC_535|Compound:2293-4129|FD-Score:4.08|P-value:1.12E-5 ID:SGTC_809|Compound:3998-0021|FD-Score:2.99|P-value:9.49E-4 ID:SGTC_894|Compound:0960-0238|FD-Score:4.31|P-value:3.77E-6 ID:SGTC_953|Compound:1188-0045|FD-Score:3.34|P-value:2.57E-4 ID:SGTC_1088|Compound:bifonazole|FD-Score:3.37|P-value:2.27E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:3.41|P-value:1.99E-4 ID:SGTC_1219|Compound:1469-0211|FD-Score:3.03|P-value:8.34E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.69|P-value:6.37E-5 ID:SGTC_1282|Compound:0886-0010|FD-Score:3.26|P-value:3.52E-4 ID:SGTC_1523|Compound:st012844|FD-Score:3.25|P-value:3.62E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:3.03|P-value:8.15E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:3.48|P-value:1.49E-4 ID:SGTC_1713|Compound:st033146|FD-Score:-3.50|P-value:1.38E-4 ID:SGTC_1715|Compound:st032160|FD-Score:3.51|P-value:1.31E-4 ID:SGTC_1719|Compound:st032227|FD-Score:-3.66|P-value:7.30E-5 ID:SGTC_1724|Compound:st037281|FD-Score:4.09|P-value:1.09E-5 ID:SGTC_1750|Compound:st081859|FD-Score:3.65|P-value:7.46E-5 ID:SGTC_1818|Compound:st052136|FD-Score:3.68|P-value:6.69E-5 ID:SGTC_1854|Compound:st056233|FD-Score:3.35|P-value:2.46E-4 ID:SGTC_1995|Compound:st072814|FD-Score:-3.44|P-value:1.76E-4 ID:SGTC_1999|Compound:st074831|FD-Score:3.83|P-value:3.41E-5 ID:SGTC_2036|Compound:5160441|FD-Score:3.14|P-value:5.51E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.20|P-value:4.42E-4 ID:SGTC_2415|Compound:st077333|FD-Score:4.00|P-value:1.62E-5 ID:SGTC_2488|Compound:5268135|FD-Score:3.60|P-value:9.11E-5 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:-3.13|P-value:5.79E-4 ID:SGTC_2523|Compound:spermine|FD-Score:3.02|P-value:8.56E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.15|P-value:5.36E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:4.65|P-value:6.88E-7 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.36|P-value:2.41E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:3.11|P-value:6.17E-4 ID:SGTC_2834|Compound:9001755|FD-Score:3.33|P-value:2.73E-4 ID:SGTC_3037|Compound:9091026|FD-Score:5.61|P-value:2.85E-9 ID:SGTC_3147|Compound:9097027|FD-Score:-3.04|P-value:7.98E-4 ID:SGTC_3152|Compound:9099253|FD-Score:5.02|P-value:9.03E-8 ID:SGTC_3157|Compound:9099274|FD-Score:3.69|P-value:6.18E-5 ID:SGTC_3162|Compound:9099653|FD-Score:3.14|P-value:5.45E-4 ID:SGTC_3188|Compound:9110789|FD-Score:4.65|P-value:6.77E-7 ID:SGTC_3203|Compound:9113543|FD-Score:3.30|P-value:3.03E-4 ID:SGTC_3208|Compound:9114115|FD-Score:-3.45|P-value:1.68E-4 ID:SGTC_3219|Compound:9131112|FD-Score:4.88|P-value:2.01E-7 ID:SGTC_3268|Compound:9137683|FD-Score:-3.06|P-value:7.37E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:5.65|P-value:2.20E-9 ID:SGTC_72|Compound:0744-0168|FD-Score:4.16|P-value:7.69E-6 ID:SGTC_74|Compound:000s-0487|FD-Score:3.07|P-value:7.14E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:3.76|P-value:4.68E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:3.57|P-value:1.04E-4 ID:SGTC_320|Compound:4092-0846|FD-Score:3.58|P-value:9.86E-5 ID:SGTC_363|Compound:0455-0021|FD-Score:-5.54|P-value:4.38E-9 ID:SGTC_369|Compound:0957-0393|FD-Score:5.56|P-value:3.83E-9 ID:SGTC_529|Compound:4076-0302|FD-Score:5.81|P-value:7.95E-10 ID:SGTC_535|Compound:2293-4129|FD-Score:4.08|P-value:1.12E-5 ID:SGTC_809|Compound:3998-0021|FD-Score:2.99|P-value:9.49E-4 ID:SGTC_894|Compound:0960-0238|FD-Score:4.31|P-value:3.77E-6 ID:SGTC_953|Compound:1188-0045|FD-Score:3.34|P-value:2.57E-4 ID:SGTC_1088|Compound:bifonazole|FD-Score:3.37|P-value:2.27E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:3.41|P-value:1.99E-4 ID:SGTC_1219|Compound:1469-0211|FD-Score:3.03|P-value:8.34E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.69|P-value:6.37E-5 ID:SGTC_1282|Compound:0886-0010|FD-Score:3.26|P-value:3.52E-4 ID:SGTC_1523|Compound:st012844|FD-Score:3.25|P-value:3.62E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:3.03|P-value:8.15E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:3.48|P-value:1.49E-4 ID:SGTC_1713|Compound:st033146|FD-Score:-3.50|P-value:1.38E-4 ID:SGTC_1715|Compound:st032160|FD-Score:3.51|P-value:1.31E-4 ID:SGTC_1719|Compound:st032227|FD-Score:-3.66|P-value:7.30E-5 ID:SGTC_1724|Compound:st037281|FD-Score:4.09|P-value:1.09E-5 ID:SGTC_1750|Compound:st081859|FD-Score:3.65|P-value:7.46E-5 ID:SGTC_1818|Compound:st052136|FD-Score:3.68|P-value:6.69E-5 ID:SGTC_1854|Compound:st056233|FD-Score:3.35|P-value:2.46E-4 ID:SGTC_1995|Compound:st072814|FD-Score:-3.44|P-value:1.76E-4 ID:SGTC_1999|Compound:st074831|FD-Score:3.83|P-value:3.41E-5 ID:SGTC_2036|Compound:5160441|FD-Score:3.14|P-value:5.51E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.20|P-value:4.42E-4 ID:SGTC_2415|Compound:st077333|FD-Score:4.00|P-value:1.62E-5 ID:SGTC_2488|Compound:5268135|FD-Score:3.60|P-value:9.11E-5 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:-3.13|P-value:5.79E-4 ID:SGTC_2523|Compound:spermine|FD-Score:3.02|P-value:8.56E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.15|P-value:5.36E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:4.65|P-value:6.88E-7 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.36|P-value:2.41E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:3.11|P-value:6.17E-4 ID:SGTC_2834|Compound:9001755|FD-Score:3.33|P-value:2.73E-4 ID:SGTC_3037|Compound:9091026|FD-Score:5.61|P-value:2.85E-9 ID:SGTC_3147|Compound:9097027|FD-Score:-3.04|P-value:7.98E-4 ID:SGTC_3152|Compound:9099253|FD-Score:5.02|P-value:9.03E-8 ID:SGTC_3157|Compound:9099274|FD-Score:3.69|P-value:6.18E-5 ID:SGTC_3162|Compound:9099653|FD-Score:3.14|P-value:5.45E-4 ID:SGTC_3188|Compound:9110789|FD-Score:4.65|P-value:6.77E-7 ID:SGTC_3203|Compound:9113543|FD-Score:3.30|P-value:3.03E-4 ID:SGTC_3208|Compound:9114115|FD-Score:-3.45|P-value:1.68E-4 ID:SGTC_3219|Compound:9131112|FD-Score:4.88|P-value:2.01E-7 ID:SGTC_3268|Compound:9137683|FD-Score:-3.06|P-value:7.37E-4

Top fitness defect scores for YPR041W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_529 4076-0302 5.81 30.3 µM 7.95E-10 mitochondrial processes 2 3.14E-9 0.75
2 SGTC_68 0986-0284 5.65 82.8 µM 2.20E-9 3 8.10E-9 0.01
3 SGTC_3037 9091026 5.61 49.5 µM 2.85E-9 NEO1-PIK1 13 1.03E-8 0.01
4 SGTC_369 0957-0393 5.56 4.5 µM 3.83E-9 cell wall signaling 2 1.35E-8 1.04
5 SGTC_3152 9099253 5.02 49.5 µM 9.03E-8 NEO1-PIK1 16 2.55E-7 0.07
6 SGTC_3219 9131112 4.88 49.5 µM 2.01E-7 Golgi 12 5.35E-7 0.15
7 SGTC_3188 9110789 4.65 49.5 µM 6.77E-7 iron homeostasis 6 1.66E-6 0.01
8 SGTC_2636 apigenin dimethyl ether 4.65 100.0 µM 6.88E-7 7 1.68E-6 0.04
9 SGTC_894 0960-0238 4.31 55.9 µM 3.77E-6 7 8.17E-6 0.72
10 SGTC_72 0744-0168 4.16 198.4 µM 7.69E-6 RPP1 & pyrimidine depletion 6 1.59E-5 0.05
11 SGTC_1724 st037281 4.09 8.3 µM 1.09E-5 13 2.20E-5 0.16
12 SGTC_535 2293-4129 4.08 48.2 µM 1.12E-5 NEO1 7 2.24E-5 0.10
13 SGTC_2415 st077333 4.00 57.7 µM 1.62E-5 16 3.17E-5 0.07
14 SGTC_1999 st074831 3.83 12.5 µM 3.41E-5 plasma membrane duress 12 6.33E-5 0.06
15 SGTC_212 3807-4682 3.76 38.8 µM 4.68E-5 tubulin folding & SWR complex 25 8.51E-5 0.08
16 SGTC_3157 9099274 3.69 49.5 µM 6.18E-5 6 1.10E-4 0.23
17 SGTC_1263 0774-1208 3.69 64.8 µM 6.37E-5 6 1.13E-4 0.13
18 SGTC_1818 st052136 3.68 56.3 µM 6.69E-5 NEO1-PIK1 21 1.18E-4 0.39
19 SGTC_1750 st081859 3.65 2.4 µM 7.46E-5 endomembrane recycling 33 1.31E-4 0.05
20 SGTC_2488 5268135 3.60 45.7 µM 9.11E-5 10 1.58E-4 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.196 2.84E-30 YJR075W HOC1 hom Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele
0.169 6.28E-23 YLR169W_d YLR169W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.169 6.89E-23 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.167 2.42E-22 YGL042C_d YGL042C_d hom Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1
0.165 5.19E-22 YGL043W DST1 hom General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress
0.150 2.57E-18 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.144 5.24E-17 YLR170C APS1 hom Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
0.144 6.33E-17 YPR029C APL4 hom Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
0.143 7.99E-17 YKR031C SPO14 hom Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions
0.142 1.57E-16 YER180C-A SLO1 hom Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO
0.140 4.72E-16 YHL019C APM2 hom Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
0.139 6.40E-16 YJL081C ARP4 het Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
0.137 1.77E-15 YBL010C_p YBL010C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
0.136 2.46E-15 YNL183C NPR1 hom Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
0.136 2.96E-15 YDR524C AGE1 hom ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif