YPR042C / PUF2

Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins

Zygosity: Homozygous strain
fixedexpanded
Profile for YPR042C / PUF2

Click on Significant Values for Screen Details ID:SGTC_112|Compound:phenothiazine|FD-Score:4.03|P-value:4.60E-5 ID:SGTC_113|Compound:0099-0221|FD-Score:3.54|P-value:2.97E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:3.65|P-value:2.03E-4 ID:SGTC_174|Compound:3553-0389|FD-Score:3.30|P-value:7.02E-4 ID:SGTC_247|Compound:mechlorethamine|FD-Score:3.47|P-value:3.89E-4 ID:SGTC_297|Compound:0986-0032|FD-Score:3.87|P-value:8.57E-5 ID:SGTC_330|Compound:1488-0319|FD-Score:3.63|P-value:2.18E-4 ID:SGTC_449|Compound:xestospongin c|FD-Score:-3.17|P-value:7.96E-4 ID:SGTC_480|Compound:am 92016|FD-Score:3.37|P-value:5.54E-4 ID:SGTC_489|Compound:paxilline|FD-Score:3.44|P-value:4.27E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:4.46|P-value:7.26E-6 ID:SGTC_542|Compound:3381-0244|FD-Score:3.25|P-value:8.29E-4 ID:SGTC_545|Compound:4218-0007|FD-Score:3.44|P-value:4.25E-4 ID:SGTC_554|Compound:4483-2211|FD-Score:3.76|P-value:1.33E-4 ID:SGTC_589|Compound:1227-0070|FD-Score:3.96|P-value:6.14E-5 ID:SGTC_661|Compound:1304-0007|FD-Score:3.78|P-value:1.23E-4 ID:SGTC_781|Compound:4335-2806|FD-Score:3.54|P-value:3.01E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:4.09|P-value:3.58E-5 ID:SGTC_877|Compound:0771-0151|FD-Score:4.88|P-value:1.06E-6 ID:SGTC_955|Compound:1216-0110|FD-Score:3.67|P-value:1.90E-4 ID:SGTC_1068|Compound:loratadine|FD-Score:3.37|P-value:5.52E-4 ID:SGTC_1098|Compound:clofazimine|FD-Score:3.55|P-value:2.94E-4 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:-3.18|P-value:7.69E-4 ID:SGTC_1109|Compound:tnp00067|FD-Score:-4.40|P-value:6.41E-6 ID:SGTC_1113|Compound:artemisinin|FD-Score:-3.41|P-value:3.47E-4 ID:SGTC_1241|Compound:0364-0022|FD-Score:3.25|P-value:8.34E-4 ID:SGTC_1268|Compound:0828-0275|FD-Score:3.59|P-value:2.54E-4 ID:SGTC_1327|Compound:1436-0082|FD-Score:4.05|P-value:4.23E-5 ID:SGTC_1373|Compound:2887-0003|FD-Score:-3.17|P-value:7.96E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:4.29|P-value:1.58E-5 ID:SGTC_1453|Compound:4237-0541|FD-Score:3.28|P-value:7.56E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:3.50|P-value:3.43E-4 ID:SGTC_1456|Compound:k015-0012|FD-Score:3.62|P-value:2.26E-4 ID:SGTC_1713|Compound:st033146|FD-Score:4.91|P-value:9.26E-7 ID:SGTC_1827|Compound:berberine|FD-Score:3.50|P-value:3.51E-4 ID:SGTC_2061|Compound:5245884|FD-Score:5.46|P-value:5.52E-8 ID:SGTC_2068|Compound:5226020|FD-Score:-3.97|P-value:4.13E-5 ID:SGTC_2180|Compound:6301957|FD-Score:3.50|P-value:3.49E-4 ID:SGTC_2182|Compound:6325192|FD-Score:4.20|P-value:2.26E-5 ID:SGTC_2183|Compound:6420625|FD-Score:3.99|P-value:5.47E-5 ID:SGTC_2201|Compound:6605497|FD-Score:4.21|P-value:2.23E-5 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:3.50|P-value:3.54E-4 ID:SGTC_2237|Compound:6667747|FD-Score:6.09|P-value:1.47E-9 ID:SGTC_2256|Compound:7954252|FD-Score:-3.41|P-value:3.57E-4 ID:SGTC_2448|Compound:5185112|FD-Score:3.39|P-value:5.18E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:5.22|P-value:1.86E-7 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:5.09|P-value:3.67E-7 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.43|P-value:8.45E-6 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:8.12|P-value:1.14E-15 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:-4.47|P-value:4.74E-6 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:4.47|P-value:7.02E-6 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-3.61|P-value:1.71E-4 ID:SGTC_2672|Compound:12a-hydroxy-5-deoxydehydromunduserone|FD-Score:3.21|P-value:9.60E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:3.37|P-value:5.51E-4 ID:SGTC_2950|Compound:9072895|FD-Score:3.43|P-value:4.40E-4 ID:SGTC_3002|Compound:9075603|FD-Score:3.39|P-value:5.08E-4 ID:SGTC_3070|Compound:9117005|FD-Score:3.26|P-value:8.01E-4 ID:SGTC_3153|Compound:9100258|FD-Score:3.47|P-value:3.88E-4 ID:SGTC_112|Compound:phenothiazine|FD-Score:4.03|P-value:4.60E-5 ID:SGTC_113|Compound:0099-0221|FD-Score:3.54|P-value:2.97E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:3.65|P-value:2.03E-4 ID:SGTC_174|Compound:3553-0389|FD-Score:3.30|P-value:7.02E-4 ID:SGTC_247|Compound:mechlorethamine|FD-Score:3.47|P-value:3.89E-4 ID:SGTC_297|Compound:0986-0032|FD-Score:3.87|P-value:8.57E-5 ID:SGTC_330|Compound:1488-0319|FD-Score:3.63|P-value:2.18E-4 ID:SGTC_449|Compound:xestospongin c|FD-Score:-3.17|P-value:7.96E-4 ID:SGTC_480|Compound:am 92016|FD-Score:3.37|P-value:5.54E-4 ID:SGTC_489|Compound:paxilline|FD-Score:3.44|P-value:4.27E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:4.46|P-value:7.26E-6 ID:SGTC_542|Compound:3381-0244|FD-Score:3.25|P-value:8.29E-4 ID:SGTC_545|Compound:4218-0007|FD-Score:3.44|P-value:4.25E-4 ID:SGTC_554|Compound:4483-2211|FD-Score:3.76|P-value:1.33E-4 ID:SGTC_589|Compound:1227-0070|FD-Score:3.96|P-value:6.14E-5 ID:SGTC_661|Compound:1304-0007|FD-Score:3.78|P-value:1.23E-4 ID:SGTC_781|Compound:4335-2806|FD-Score:3.54|P-value:3.01E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:4.09|P-value:3.58E-5 ID:SGTC_877|Compound:0771-0151|FD-Score:4.88|P-value:1.06E-6 ID:SGTC_955|Compound:1216-0110|FD-Score:3.67|P-value:1.90E-4 ID:SGTC_1068|Compound:loratadine|FD-Score:3.37|P-value:5.52E-4 ID:SGTC_1098|Compound:clofazimine|FD-Score:3.55|P-value:2.94E-4 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:-3.18|P-value:7.69E-4 ID:SGTC_1109|Compound:tnp00067|FD-Score:-4.40|P-value:6.41E-6 ID:SGTC_1113|Compound:artemisinin|FD-Score:-3.41|P-value:3.47E-4 ID:SGTC_1241|Compound:0364-0022|FD-Score:3.25|P-value:8.34E-4 ID:SGTC_1268|Compound:0828-0275|FD-Score:3.59|P-value:2.54E-4 ID:SGTC_1327|Compound:1436-0082|FD-Score:4.05|P-value:4.23E-5 ID:SGTC_1373|Compound:2887-0003|FD-Score:-3.17|P-value:7.96E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:4.29|P-value:1.58E-5 ID:SGTC_1453|Compound:4237-0541|FD-Score:3.28|P-value:7.56E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:3.50|P-value:3.43E-4 ID:SGTC_1456|Compound:k015-0012|FD-Score:3.62|P-value:2.26E-4 ID:SGTC_1713|Compound:st033146|FD-Score:4.91|P-value:9.26E-7 ID:SGTC_1827|Compound:berberine|FD-Score:3.50|P-value:3.51E-4 ID:SGTC_2061|Compound:5245884|FD-Score:5.46|P-value:5.52E-8 ID:SGTC_2068|Compound:5226020|FD-Score:-3.97|P-value:4.13E-5 ID:SGTC_2180|Compound:6301957|FD-Score:3.50|P-value:3.49E-4 ID:SGTC_2182|Compound:6325192|FD-Score:4.20|P-value:2.26E-5 ID:SGTC_2183|Compound:6420625|FD-Score:3.99|P-value:5.47E-5 ID:SGTC_2201|Compound:6605497|FD-Score:4.21|P-value:2.23E-5 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:3.50|P-value:3.54E-4 ID:SGTC_2237|Compound:6667747|FD-Score:6.09|P-value:1.47E-9 ID:SGTC_2256|Compound:7954252|FD-Score:-3.41|P-value:3.57E-4 ID:SGTC_2448|Compound:5185112|FD-Score:3.39|P-value:5.18E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:5.22|P-value:1.86E-7 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:5.09|P-value:3.67E-7 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.43|P-value:8.45E-6 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:8.12|P-value:1.14E-15 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:-4.47|P-value:4.74E-6 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:4.47|P-value:7.02E-6 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-3.61|P-value:1.71E-4 ID:SGTC_2672|Compound:12a-hydroxy-5-deoxydehydromunduserone|FD-Score:3.21|P-value:9.60E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:3.37|P-value:5.51E-4 ID:SGTC_2950|Compound:9072895|FD-Score:3.43|P-value:4.40E-4 ID:SGTC_3002|Compound:9075603|FD-Score:3.39|P-value:5.08E-4 ID:SGTC_3070|Compound:9117005|FD-Score:3.26|P-value:8.01E-4 ID:SGTC_3153|Compound:9100258|FD-Score:3.47|P-value:3.88E-4

Top fitness defect scores for YPR042C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2588 indole-3-carbinol 8.12 100.0 µM 1.14E-15 6 2.28E-16
2 SGTC_2237 6667747 6.09 47.2 µM 1.47E-9 4 5.51E-10
3 SGTC_2061 5245884 5.46 81.0 µM 5.52E-8 tubulin folding & SWR complex 7 2.44E-8
4 SGTC_2504 purpurogallin 5.22 52.3 µM 1.86E-7 RPP1 & pyrimidine depletion 5 8.73E-8
5 SGTC_2553 4-methylesculetin 5.09 85.1 µM 3.67E-7 5 1.77E-7
6 SGTC_1713 st033146 4.91 12.8 µM 9.26E-7 10 4.67E-7
7 SGTC_877 0771-0151 4.88 33.1 µM 1.06E-6 7 5.38E-7
8 SGTC_2631 bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone 4.47 100.0 µM 7.02E-6 24 3.89E-6
9 SGTC_513 4245-1575 4.46 41.7 µM 7.26E-6 unfolded protein response 92 4.03E-6
10 SGTC_2582 agaric acid 4.43 100.0 µM 8.45E-6 redox potentiating 265 4.72E-6
11 SGTC_1450 4112-4260 4.29 37.6 µM 1.58E-5 calcium & mitochondrial duress 19 9.08E-6
12 SGTC_2201 6605497 4.21 169.9 µM 2.23E-5 ergosterol biosynthesis 6 1.30E-5
13 SGTC_2182 6325192 4.20 200.0 µM 2.26E-5 Golgi 11 1.32E-5
14 SGTC_872 0588-0139 4.09 234.0 µM 3.58E-5 29 2.14E-5
15 SGTC_1327 1436-0082 4.05 2.2 µM 4.23E-5 19 2.55E-5
16 SGTC_112 phenothiazine 4.03 287.2 µM 4.60E-5 unfolded protein response 11 2.79E-5
17 SGTC_2183 6420625 3.99 130.5 µM 5.47E-5 mitochondrial processes 56 3.34E-5
18 SGTC_589 1227-0070 3.96 34.1 µM 6.14E-5 29 3.77E-5
19 SGTC_297 0986-0032 3.87 24.0 µM 8.57E-5 60S ribosome export 7 5.34E-5
20 SGTC_661 1304-0007 3.78 33.6 µM 1.23E-4 26 7.83E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.110 1.54E-10 YNL106C INP52 hom Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain
0.104 1.81E-9 YML103C NUP188 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.101 4.14E-9 YDL241W_p YDL241W_p hom Putative protein of unknown function; YDL241W is not an essential gene
0.099 9.42E-9 YBR259W YBR259W hom Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress
0.090 1.97E-7 YOR049C RSB1 hom Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane
0.088 3.26E-7 YOR202W HIS3 hom Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p
0.087 5.23E-7 YKL162C-A_d YKL162C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.085 8.49E-7 YLR433C CNA1 hom Calcineurin A; one isoform (the other is CMP2) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1
0.085 9.41E-7 YPL232W SSO1 hom Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p
0.084 1.19E-6 YOL013C HRD1 hom Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon
0.083 1.32E-6 YNL027W CRZ1 hom Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress
0.083 1.66E-6 YDR031W MIC14 hom Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
0.082 1.88E-6 YML052W SUR7 hom Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants
0.081 2.80E-6 YHR013C ARD1 hom Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress
0.080 3.36E-6 YOR161C-C_p YOR161C-C_p hom Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching