HIPHOP chemogenomics database

YPR052C / NHP6A

High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain

fixedexpanded
Profile for YPR052C / NHP6A

Click on Significant Values for Screen Details

Top fitness defect scores for YPR052C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2691	st077356	6.06	60.2 µM	8.09E-11		7	6.63E-10
2	SGTC_58	0957-0393	5.50	9.0 µM	3.34E-9	cell wall	243	1.90E-8
3	SGTC_1600	coumarin 106	4.88	71.1 µM	1.33E-7		9	5.27E-7
4	SGTC_319	1348-1596	4.43	45.5 µM	1.50E-6	RSC & ERG11	12	4.70E-6
5	SGTC_1596	piperlongumine	4.43	63.0 µM	1.51E-6		7	4.75E-6
6	SGTC_2225	6654152	4.24	15.4 µM	3.84E-6		11	1.10E-5
7	SGTC_411	enrofloxacin	4.23	100.0 µM	4.18E-6	DNA damage response	8	1.19E-5
8	SGTC_1534	6-azauridine	4.14	81.6 µM	6.49E-6	RNA pol III & RNase P/MRP	19	1.77E-5
9	SGTC_1798	st045719	4.09	61.0 µM	8.10E-6		10	2.16E-5
10	SGTC_1302	1159-0335	3.93	255.0 µM	1.69E-5		14	4.20E-5
11	SGTC_2065	5221596	3.89	34.3 µM	2.09E-5		18	5.10E-5
12	SGTC_2350	9023895	3.85	38.9 µM	2.42E-5	ergosterol biosynthesis	34	5.83E-5
13	SGTC_431	k089-0097	3.78	107.0 µM	3.36E-5	iron homeostasis	53	7.82E-5
14	SGTC_2623	humulene	3.76	100.0 µM	3.71E-5		25	8.56E-5
15	SGTC_190	3970-0790	3.68	3.7 µM	5.11E-5		10	1.14E-4
16	SGTC_169	k015-0017	3.67	16.0 µM	5.36E-5		27	1.19E-4
17	SGTC_1109	tnp00067	3.58	928.3 nM	7.85E-5		24	1.69E-4
18	SGTC_590	k048-0076	3.49	22.4 µM	1.16E-4	RSC & ERG11	19	2.40E-4
19	SGTC_2063	5246145	3.48	200.0 µM	1.19E-4		38	2.46E-4
20	SGTC_2887	9044784	3.45	58.4 µM	1.37E-4	fatty acid desaturase (OLE1)	56	2.80E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.171	2.26E-23	YCR012W	PGK1	het	3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis
0.171	2.53E-23	YOR231W	MKK1	hom	MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.170	3.53E-23	YIL160C	POT1	hom	3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.156	8.55E-20	YDR205W	MSC2	hom	Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.143	8.64E-17	YAL015C	NTG1	hom	DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.128	8.15E-14	YBR120C	CBP6	hom	Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.125	4.04E-13	YKL115C_d	YKL115C_d	hom	Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1
0.123	7.79E-13	YNL215W	IES2	hom	Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
0.123	8.39E-13	YDL189W	RBS1	hom	Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain
0.123	9.35E-13	YKR042W	UTH1	hom	Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication
0.121	1.62E-12	YIL123W	SIM1	hom	Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated
0.121	2.22E-12	YDR537C_d	YDR537C_d	hom	Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W
0.118	7.27E-12	YGR058W	PEF1	hom	Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.116	1.85E-11	YPR097W	YPR097W	hom	Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.115	2.90E-11	YMR133W	REC114	hom	Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division