YPR054W / SMK1

Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p

Zygosity: Homozygous strain
fixedexpanded
Profile for YPR054W / SMK1

Click on Significant Values for Screen Details ID:SGTC_17|Compound:0960-0276|FD-Score:3.31|P-value:8.13E-5 ID:SGTC_35|Compound:0133-0054|FD-Score:2.79|P-value:7.62E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:-2.72|P-value:9.45E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:-2.79|P-value:7.14E-4 ID:SGTC_92|Compound:0063-0024|FD-Score:4.29|P-value:5.10E-7 ID:SGTC_175|Compound:3793-3120|FD-Score:-3.47|P-value:3.77E-5 ID:SGTC_176|Compound:1486-1328|FD-Score:-3.21|P-value:1.24E-4 ID:SGTC_179|Compound:1273-0063|FD-Score:3.86|P-value:5.60E-6 ID:SGTC_184|Compound:1494-0393|FD-Score:4.11|P-value:1.45E-6 ID:SGTC_284|Compound:0988-0104|FD-Score:3.67|P-value:1.47E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:-2.92|P-value:4.33E-4 ID:SGTC_370|Compound:0180-0345|FD-Score:3.54|P-value:2.84E-5 ID:SGTC_619|Compound:1652-1634|FD-Score:3.47|P-value:3.86E-5 ID:SGTC_763|Compound:3453-1109|FD-Score:4.98|P-value:7.21E-9 ID:SGTC_773|Compound:4358-1457|FD-Score:5.51|P-value:1.77E-10 ID:SGTC_830|Compound:4076-0286|FD-Score:2.83|P-value:6.48E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:2.80|P-value:7.12E-4 ID:SGTC_980|Compound:1473-0395|FD-Score:3.18|P-value:1.49E-4 ID:SGTC_1015|Compound:4130-4525|FD-Score:3.61|P-value:2.04E-5 ID:SGTC_1100|Compound:amiodarone|FD-Score:-2.79|P-value:7.07E-4 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:-2.92|P-value:4.20E-4 ID:SGTC_1146|Compound:3579-1023|FD-Score:2.95|P-value:3.94E-4 ID:SGTC_1164|Compound:k060-0068|FD-Score:5.82|P-value:1.73E-11 ID:SGTC_1440|Compound:4100-1550|FD-Score:2.90|P-value:4.76E-4 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:3.79|P-value:8.17E-6 ID:SGTC_1596|Compound:piperlongumine|FD-Score:3.28|P-value:9.60E-5 ID:SGTC_1748|Compound:st037455|FD-Score:3.53|P-value:2.92E-5 ID:SGTC_1755|Compound:st041960|FD-Score:2.87|P-value:5.38E-4 ID:SGTC_1910|Compound:st062352|FD-Score:8.03|P-value:2.94E-20 ID:SGTC_1969|Compound:st069479|FD-Score:2.73|P-value:9.41E-4 ID:SGTC_2011|Compound:4012611|FD-Score:4.82|P-value:2.04E-8 ID:SGTC_2101|Compound:5377231|FD-Score:3.04|P-value:2.76E-4 ID:SGTC_2142|Compound:5622915|FD-Score:3.40|P-value:5.58E-5 ID:SGTC_2240|Compound:6690706|FD-Score:2.72|P-value:9.79E-4 ID:SGTC_2246|Compound:7293507|FD-Score:3.19|P-value:1.43E-4 ID:SGTC_2261|Compound:9010538|FD-Score:4.88|P-value:1.40E-8 ID:SGTC_2309|Compound:7664088|FD-Score:3.39|P-value:5.68E-5 ID:SGTC_2358|Compound:9037157|FD-Score:3.09|P-value:2.23E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:2.74|P-value:9.26E-4 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:3.00|P-value:3.19E-4 ID:SGTC_2463|Compound:5473429|FD-Score:4.66|P-value:5.57E-8 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:-2.97|P-value:3.53E-4 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:3.73|P-value:1.11E-5 ID:SGTC_2570|Compound:licareol|FD-Score:-4.17|P-value:9.65E-7 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:-5.06|P-value:3.79E-9 ID:SGTC_2640|Compound:pentamidine|FD-Score:2.83|P-value:6.44E-4 ID:SGTC_2702|Compound:st077768|FD-Score:-2.86|P-value:5.36E-4 ID:SGTC_3103|Compound:9120822|FD-Score:3.08|P-value:2.33E-4 ID:SGTC_3151|Compound:9099821|FD-Score:-3.24|P-value:1.06E-4 ID:SGTC_3199|Compound:9111318|FD-Score:3.08|P-value:2.30E-4 ID:SGTC_3201|Compound:9113999|FD-Score:-4.63|P-value:6.39E-8 ID:SGTC_3208|Compound:9114115|FD-Score:4.57|P-value:9.64E-8 ID:SGTC_3259|Compound:9138485|FD-Score:3.25|P-value:1.10E-4 ID:SGTC_3294|Compound:9122953|FD-Score:2.92|P-value:4.44E-4 ID:SGTC_17|Compound:0960-0276|FD-Score:3.31|P-value:8.13E-5 ID:SGTC_35|Compound:0133-0054|FD-Score:2.79|P-value:7.62E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:-2.72|P-value:9.45E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:-2.79|P-value:7.14E-4 ID:SGTC_92|Compound:0063-0024|FD-Score:4.29|P-value:5.10E-7 ID:SGTC_175|Compound:3793-3120|FD-Score:-3.47|P-value:3.77E-5 ID:SGTC_176|Compound:1486-1328|FD-Score:-3.21|P-value:1.24E-4 ID:SGTC_179|Compound:1273-0063|FD-Score:3.86|P-value:5.60E-6 ID:SGTC_184|Compound:1494-0393|FD-Score:4.11|P-value:1.45E-6 ID:SGTC_284|Compound:0988-0104|FD-Score:3.67|P-value:1.47E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:-2.92|P-value:4.33E-4 ID:SGTC_370|Compound:0180-0345|FD-Score:3.54|P-value:2.84E-5 ID:SGTC_619|Compound:1652-1634|FD-Score:3.47|P-value:3.86E-5 ID:SGTC_763|Compound:3453-1109|FD-Score:4.98|P-value:7.21E-9 ID:SGTC_773|Compound:4358-1457|FD-Score:5.51|P-value:1.77E-10 ID:SGTC_830|Compound:4076-0286|FD-Score:2.83|P-value:6.48E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:2.80|P-value:7.12E-4 ID:SGTC_980|Compound:1473-0395|FD-Score:3.18|P-value:1.49E-4 ID:SGTC_1015|Compound:4130-4525|FD-Score:3.61|P-value:2.04E-5 ID:SGTC_1100|Compound:amiodarone|FD-Score:-2.79|P-value:7.07E-4 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:-2.92|P-value:4.20E-4 ID:SGTC_1146|Compound:3579-1023|FD-Score:2.95|P-value:3.94E-4 ID:SGTC_1164|Compound:k060-0068|FD-Score:5.82|P-value:1.73E-11 ID:SGTC_1440|Compound:4100-1550|FD-Score:2.90|P-value:4.76E-4 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:3.79|P-value:8.17E-6 ID:SGTC_1596|Compound:piperlongumine|FD-Score:3.28|P-value:9.60E-5 ID:SGTC_1748|Compound:st037455|FD-Score:3.53|P-value:2.92E-5 ID:SGTC_1755|Compound:st041960|FD-Score:2.87|P-value:5.38E-4 ID:SGTC_1910|Compound:st062352|FD-Score:8.03|P-value:2.94E-20 ID:SGTC_1969|Compound:st069479|FD-Score:2.73|P-value:9.41E-4 ID:SGTC_2011|Compound:4012611|FD-Score:4.82|P-value:2.04E-8 ID:SGTC_2101|Compound:5377231|FD-Score:3.04|P-value:2.76E-4 ID:SGTC_2142|Compound:5622915|FD-Score:3.40|P-value:5.58E-5 ID:SGTC_2240|Compound:6690706|FD-Score:2.72|P-value:9.79E-4 ID:SGTC_2246|Compound:7293507|FD-Score:3.19|P-value:1.43E-4 ID:SGTC_2261|Compound:9010538|FD-Score:4.88|P-value:1.40E-8 ID:SGTC_2309|Compound:7664088|FD-Score:3.39|P-value:5.68E-5 ID:SGTC_2358|Compound:9037157|FD-Score:3.09|P-value:2.23E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:2.74|P-value:9.26E-4 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:3.00|P-value:3.19E-4 ID:SGTC_2463|Compound:5473429|FD-Score:4.66|P-value:5.57E-8 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:-2.97|P-value:3.53E-4 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:3.73|P-value:1.11E-5 ID:SGTC_2570|Compound:licareol|FD-Score:-4.17|P-value:9.65E-7 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:-5.06|P-value:3.79E-9 ID:SGTC_2640|Compound:pentamidine|FD-Score:2.83|P-value:6.44E-4 ID:SGTC_2702|Compound:st077768|FD-Score:-2.86|P-value:5.36E-4 ID:SGTC_3103|Compound:9120822|FD-Score:3.08|P-value:2.33E-4 ID:SGTC_3151|Compound:9099821|FD-Score:-3.24|P-value:1.06E-4 ID:SGTC_3199|Compound:9111318|FD-Score:3.08|P-value:2.30E-4 ID:SGTC_3201|Compound:9113999|FD-Score:-4.63|P-value:6.39E-8 ID:SGTC_3208|Compound:9114115|FD-Score:4.57|P-value:9.64E-8 ID:SGTC_3259|Compound:9138485|FD-Score:3.25|P-value:1.10E-4 ID:SGTC_3294|Compound:9122953|FD-Score:2.92|P-value:4.44E-4

Top fitness defect scores for YPR054W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1910 st062352 8.03 60.4 µM 2.94E-20 4 4.89E-16
2 SGTC_1164 k060-0068 5.82 93.7 µM 1.73E-11 5 2.98E-9
3 SGTC_773 4358-1457 5.51 44.6 µM 1.77E-10 15 1.81E-8
4 SGTC_763 3453-1109 4.98 128.0 µM 7.21E-9 5 3.20E-7
5 SGTC_2261 9010538 4.88 184.7 µM 1.40E-8 7 5.37E-7
6 SGTC_2011 4012611 4.82 177.0 µM 2.04E-8 mitochondrial processes 13 7.19E-7
7 SGTC_2463 5473429 4.66 200.0 µM 5.57E-8 ERG2 7 1.57E-6
8 SGTC_3208 9114115 4.57 49.5 µM 9.64E-8 23 2.40E-6
9 SGTC_92 0063-0024 4.29 660.9 µM 5.10E-7 5 8.79E-6
10 SGTC_184 1494-0393 4.11 24.7 µM 1.45E-6 53 1.98E-5
11 SGTC_179 1273-0063 3.86 6.8 µM 5.60E-6 23 5.69E-5
12 SGTC_1541 2',3',6-trimethoxyflavone 3.79 64.0 µM 8.17E-6 18 7.64E-5
13 SGTC_2500 5-alpha-androstan-3,17-dione 3.73 61.4 µM 1.11E-5 45 9.72E-5
14 SGTC_284 0988-0104 3.67 32.6 µM 1.47E-5 heme requiring 15 1.21E-4
15 SGTC_1015 4130-4525 3.61 82.0 µM 2.04E-5 60S ribosome export 32 1.56E-4
16 SGTC_370 0180-0345 3.54 38.7 µM 2.84E-5 DNA intercalators 21 2.02E-4
17 SGTC_1748 st037455 3.53 53.6 µM 2.92E-5 46 2.06E-4
18 SGTC_619 1652-1634 3.47 108.0 µM 3.86E-5 PDR1 32 2.57E-4
19 SGTC_2142 5622915 3.40 118.5 µM 5.58E-5 22 3.43E-4
20 SGTC_2309 7664088 3.39 163.9 µM 5.68E-5 71 3.47E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.145 2.83E-17 YML012W ERV25 hom Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport
0.143 1.01E-16 YPL035C_d YPL035C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene
0.128 1.05E-13 YAL043C PTA1 het Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII
0.127 1.36E-13 YJL093C TOK1 hom Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin
0.117 1.03E-11 YNL120C_d YNL120C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene
0.114 4.13E-11 YOR108W LEU9 hom Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication
0.110 1.40E-10 YER172C BRR2 het RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD
0.107 5.42E-10 YCL026C-A FRM2 hom Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin
0.105 1.13E-9 YOR361C PRT1 het eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes
0.102 3.20E-9 YPR201W ARR3 hom Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite
0.094 4.67E-8 YPL105C SYH1 hom Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication
0.093 6.30E-8 YPR058W YMC1 hom Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC1 has a paralog, YMC2, that arose from the whole genome duplication
0.093 7.51E-8 YGR242W_d YGR242W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C
0.092 1.03E-7 YER087C-B SBH1 hom Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p
0.089 2.52E-7 YLR053C_p YLR053C_p hom Putative protein of unknown function