YPR056W / TFB4

Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPR056W / TFB4

Click on Significant Values for Screen Details ID:SGTC_219|Compound:mitoxanthrone|FD-Score:4.33|P-value:3.43E-6 ID:SGTC_370|Compound:0180-0345|FD-Score:3.19|P-value:4.45E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:3.12|P-value:5.74E-4 ID:SGTC_630|Compound:4356-0227|FD-Score:-3.04|P-value:9.61E-4 ID:SGTC_815|Compound:1582-0027|FD-Score:3.59|P-value:9.50E-5 ID:SGTC_821|Compound:1114-0266|FD-Score:4.82|P-value:2.85E-7 ID:SGTC_845|Compound:0330-0138|FD-Score:3.01|P-value:8.56E-4 ID:SGTC_885|Compound:0865-0106|FD-Score:3.29|P-value:3.02E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:3.52|P-value:1.23E-4 ID:SGTC_948|Compound:3448-0295|FD-Score:-3.64|P-value:9.87E-5 ID:SGTC_960|Compound:1222-0186|FD-Score:3.43|P-value:1.77E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:-4.05|P-value:1.69E-5 ID:SGTC_1266|Compound:0828-0227|FD-Score:-3.68|P-value:8.34E-5 ID:SGTC_1293|Compound:1000-1278|FD-Score:3.31|P-value:2.87E-4 ID:SGTC_1323|Compound:1348-1351|FD-Score:4.38|P-value:2.70E-6 ID:SGTC_1464|Compound:k208-0027|FD-Score:3.19|P-value:4.43E-4 ID:SGTC_1676|Compound:st018489|FD-Score:-3.21|P-value:5.17E-4 ID:SGTC_1684|Compound:st024538|FD-Score:-4.20|P-value:8.61E-6 ID:SGTC_1685|Compound:st024049|FD-Score:-3.20|P-value:5.32E-4 ID:SGTC_1696|Compound:st025591|FD-Score:-3.33|P-value:3.31E-4 ID:SGTC_1783|Compound:st039459|FD-Score:3.21|P-value:4.05E-4 ID:SGTC_1907|Compound:st060210|FD-Score:-3.67|P-value:8.54E-5 ID:SGTC_2168|Compound:5727979|FD-Score:-3.17|P-value:5.94E-4 ID:SGTC_2288|Compound:7946289|FD-Score:-3.52|P-value:1.61E-4 ID:SGTC_2471|Compound:5466956|FD-Score:4.25|P-value:5.11E-6 ID:SGTC_2507|Compound:purpurin|FD-Score:3.14|P-value:5.26E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.50|P-value:1.74E-4 ID:SGTC_2648|Compound:atorvastatin|FD-Score:2.97|P-value:9.73E-4 ID:SGTC_2780|Compound:5215298|FD-Score:-4.00|P-value:2.14E-5 ID:SGTC_2837|Compound:9008476|FD-Score:-4.22|P-value:7.93E-6 ID:SGTC_2925|Compound:9010536|FD-Score:-3.40|P-value:2.55E-4 ID:SGTC_2960|Compound:9079389|FD-Score:-5.04|P-value:1.19E-7 ID:SGTC_2964|Compound:9083345|FD-Score:2.99|P-value:9.08E-4 ID:SGTC_3057|Compound:9095946|FD-Score:4.07|P-value:1.16E-5 ID:SGTC_3197|Compound:9113331|FD-Score:3.40|P-value:2.02E-4 ID:SGTC_3216|Compound:9129716|FD-Score:-3.33|P-value:3.33E-4 ID:SGTC_3225|Compound:9130819|FD-Score:3.60|P-value:8.78E-5 ID:SGTC_3263|Compound:9137018|FD-Score:-3.46|P-value:2.01E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:4.33|P-value:3.43E-6 ID:SGTC_370|Compound:0180-0345|FD-Score:3.19|P-value:4.45E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:3.12|P-value:5.74E-4 ID:SGTC_630|Compound:4356-0227|FD-Score:-3.04|P-value:9.61E-4 ID:SGTC_815|Compound:1582-0027|FD-Score:3.59|P-value:9.50E-5 ID:SGTC_821|Compound:1114-0266|FD-Score:4.82|P-value:2.85E-7 ID:SGTC_845|Compound:0330-0138|FD-Score:3.01|P-value:8.56E-4 ID:SGTC_885|Compound:0865-0106|FD-Score:3.29|P-value:3.02E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:3.52|P-value:1.23E-4 ID:SGTC_948|Compound:3448-0295|FD-Score:-3.64|P-value:9.87E-5 ID:SGTC_960|Compound:1222-0186|FD-Score:3.43|P-value:1.77E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:-4.05|P-value:1.69E-5 ID:SGTC_1266|Compound:0828-0227|FD-Score:-3.68|P-value:8.34E-5 ID:SGTC_1293|Compound:1000-1278|FD-Score:3.31|P-value:2.87E-4 ID:SGTC_1323|Compound:1348-1351|FD-Score:4.38|P-value:2.70E-6 ID:SGTC_1464|Compound:k208-0027|FD-Score:3.19|P-value:4.43E-4 ID:SGTC_1676|Compound:st018489|FD-Score:-3.21|P-value:5.17E-4 ID:SGTC_1684|Compound:st024538|FD-Score:-4.20|P-value:8.61E-6 ID:SGTC_1685|Compound:st024049|FD-Score:-3.20|P-value:5.32E-4 ID:SGTC_1696|Compound:st025591|FD-Score:-3.33|P-value:3.31E-4 ID:SGTC_1783|Compound:st039459|FD-Score:3.21|P-value:4.05E-4 ID:SGTC_1907|Compound:st060210|FD-Score:-3.67|P-value:8.54E-5 ID:SGTC_2168|Compound:5727979|FD-Score:-3.17|P-value:5.94E-4 ID:SGTC_2288|Compound:7946289|FD-Score:-3.52|P-value:1.61E-4 ID:SGTC_2471|Compound:5466956|FD-Score:4.25|P-value:5.11E-6 ID:SGTC_2507|Compound:purpurin|FD-Score:3.14|P-value:5.26E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.50|P-value:1.74E-4 ID:SGTC_2648|Compound:atorvastatin|FD-Score:2.97|P-value:9.73E-4 ID:SGTC_2780|Compound:5215298|FD-Score:-4.00|P-value:2.14E-5 ID:SGTC_2837|Compound:9008476|FD-Score:-4.22|P-value:7.93E-6 ID:SGTC_2925|Compound:9010536|FD-Score:-3.40|P-value:2.55E-4 ID:SGTC_2960|Compound:9079389|FD-Score:-5.04|P-value:1.19E-7 ID:SGTC_2964|Compound:9083345|FD-Score:2.99|P-value:9.08E-4 ID:SGTC_3057|Compound:9095946|FD-Score:4.07|P-value:1.16E-5 ID:SGTC_3197|Compound:9113331|FD-Score:3.40|P-value:2.02E-4 ID:SGTC_3216|Compound:9129716|FD-Score:-3.33|P-value:3.33E-4 ID:SGTC_3225|Compound:9130819|FD-Score:3.60|P-value:8.78E-5 ID:SGTC_3263|Compound:9137018|FD-Score:-3.46|P-value:2.01E-4

Top fitness defect scores for YPR056W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_821 1114-0266 4.82 29.2 µM 2.85E-7 2 7.32E-7 0.23
2 SGTC_1323 1348-1351 4.38 11.2 µM 2.70E-6 2 6.00E-6 0.49
3 SGTC_219 mitoxanthrone 4.33 164.4 µM 3.43E-6 DNA intercalators 11 7.53E-6 0.11
4 SGTC_2471 5466956 4.25 24.8 µM 5.11E-6 mitochondrial processes 13 1.09E-5 0.04
5 SGTC_3057 9095946 4.07 49.5 µM 1.16E-5 Golgi 4 2.34E-5 0.21
6 SGTC_3225 9130819 3.60 49.5 µM 8.78E-5 amide catabolism 13 1.56E-4 0.16
7 SGTC_815 1582-0027 3.59 36.9 µM 9.50E-5 6 1.68E-4 0.10
8 SGTC_944 3370-0224 3.52 231.0 µM 1.23E-4 9 2.14E-4 0.08
9 SGTC_960 1222-0186 3.43 7.7 µM 1.77E-4 15 3.00E-4 0.00
10 SGTC_3197 9113331 3.40 49.5 µM 2.02E-4 17 3.40E-4 0.09
11 SGTC_1293 1000-1278 3.31 195.0 µM 2.87E-4 15 4.73E-4 0.20
12 SGTC_885 0865-0106 3.29 16.8 µM 3.02E-4 TSC3-RPN4 25 4.95E-4 0.02
13 SGTC_1783 st039459 3.21 87.5 µM 4.05E-4 Golgi 16 6.52E-4 0.03
14 SGTC_1464 k208-0027 3.19 110.0 µM 4.43E-4 9 7.10E-4 0.04
15 SGTC_370 0180-0345 3.19 38.7 µM 4.45E-4 DNA intercalators 14 7.12E-4 0.12
16 SGTC_2507 purpurin 3.14 79.7 µM 5.26E-4 copper-dependent oxidative stress 12 8.33E-4 0.01
17 SGTC_515 4092-1064 3.12 102.0 µM 5.74E-4 plasma membrane duress 29 9.05E-4 0.00
18 SGTC_845 0330-0138 3.01 4.5 µM 8.56E-4 18 0.00131 0.10
19 SGTC_2964 9083345 2.99 53.4 µM 9.08E-4 30 0.00139 0.02
20 SGTC_2648 atorvastatin 2.97 78.7 µM 9.73E-4 azole & statin 17 0.00148 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.137 1.48E-15 YJR052W RAD7 hom Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex
0.108 3.35E-10 YDR382W RPP2B hom Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm
0.100 6.59E-9 YGR069W_d YGR069W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.100 6.83E-9 YBR024W SCO2 hom Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication
0.094 4.97E-8 YGR036C CAX4 hom Dolichyl pyrophosphate (Dol-P-P) phosphatase with a luminally oriented active site in the ER, cleaves the anhydride linkage in Dol-P-P, required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation
0.092 8.27E-8 YJL146W IDS2 hom Protein involved in modulation of Ime2p activity during meiosis, appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation
0.088 3.30E-7 YPL262W FUM1 hom Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria
0.084 9.58E-7 YDR406W PDR15 hom Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element
0.083 1.49E-6 YJR115W_p YJR115W_p hom Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication
0.080 3.51E-6 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.080 3.56E-6 YKL137W CMC1 hom Evolutionarily conserved copper-binding protein of the mitochondrial intermembrane space, may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif
0.079 4.61E-6 YGR218W CRM1 het Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin
0.077 8.98E-6 YOL020W TAT2 hom High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance
0.076 9.44E-6 YJR135C MCM22 hom Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4
0.076 1.12E-5 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm