YPR066W / UBA3

Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern

Zygosity: Homozygous strain
fixedexpanded
Profile for YPR066W / UBA3

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:3.88|P-value:1.08E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:4.05|P-value:5.66E-5 ID:SGTC_72|Compound:0744-0168|FD-Score:-3.56|P-value:3.46E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:4.13|P-value:4.09E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:-4.56|P-value:6.96E-6 ID:SGTC_117|Compound:lucigenin|FD-Score:3.58|P-value:3.17E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:5.87|P-value:1.11E-8 ID:SGTC_298|Compound:1391-0729|FD-Score:-4.00|P-value:6.85E-5 ID:SGTC_359|Compound:0081-0058|FD-Score:6.56|P-value:2.04E-10 ID:SGTC_426|Compound:0988-0037|FD-Score:3.59|P-value:3.12E-4 ID:SGTC_495|Compound:decylubiquinone|FD-Score:-3.59|P-value:3.14E-4 ID:SGTC_564|Compound:3555-0129|FD-Score:-3.78|P-value:1.62E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:3.63|P-value:2.66E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:3.26|P-value:9.45E-4 ID:SGTC_588|Compound:3970-1144|FD-Score:-4.16|P-value:3.78E-5 ID:SGTC_594|Compound:1487-1584|FD-Score:-5.31|P-value:2.11E-7 ID:SGTC_631|Compound:k072-0230|FD-Score:-3.74|P-value:1.84E-4 ID:SGTC_667|Compound:1498-1044|FD-Score:3.98|P-value:7.34E-5 ID:SGTC_760|Compound:0568-0725|FD-Score:3.92|P-value:9.25E-5 ID:SGTC_772|Compound:k072-0172|FD-Score:4.49|P-value:9.38E-6 ID:SGTC_816|Compound:0325-0417|FD-Score:4.82|P-value:2.20E-6 ID:SGTC_817|Compound:1391-0715|FD-Score:-3.87|P-value:1.15E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:-5.52|P-value:7.29E-8 ID:SGTC_824|Compound:1304-0008|FD-Score:4.01|P-value:6.67E-5 ID:SGTC_1082|Compound:camptothecin|FD-Score:7.84|P-value:3.84E-14 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:9.74|P-value:8.10E-21 ID:SGTC_1278|Compound:0874-0585|FD-Score:3.44|P-value:5.15E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:3.33|P-value:7.47E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:-4.45|P-value:1.13E-5 ID:SGTC_1707|Compound:st032323|FD-Score:4.09|P-value:4.89E-5 ID:SGTC_1775|Compound:st081588|FD-Score:5.36|P-value:1.63E-7 ID:SGTC_1842|Compound:st055362|FD-Score:3.43|P-value:5.47E-4 ID:SGTC_2023|Compound:5144124|FD-Score:3.40|P-value:5.97E-4 ID:SGTC_2359|Compound:9039622|FD-Score:4.75|P-value:2.94E-6 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:3.87|P-value:1.13E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:-3.98|P-value:7.41E-5 ID:SGTC_2589|Compound:lobaric acid|FD-Score:4.23|P-value:2.81E-5 ID:SGTC_3022|Compound:9087993|FD-Score:3.26|P-value:9.48E-4 ID:SGTC_3023|Compound:9089636|FD-Score:4.32|P-value:1.90E-5 ID:SGTC_3036|Compound:9090181|FD-Score:5.38|P-value:1.48E-7 ID:SGTC_3082|Compound:9117008|FD-Score:4.21|P-value:3.05E-5 ID:SGTC_3215|Compound:9129942|FD-Score:-3.74|P-value:1.86E-4 ID:SGTC_3299|Compound:9121982|FD-Score:4.87|P-value:1.70E-6 ID:SGTC_36|Compound:0335-0881|FD-Score:3.88|P-value:1.08E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:4.05|P-value:5.66E-5 ID:SGTC_72|Compound:0744-0168|FD-Score:-3.56|P-value:3.46E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:4.13|P-value:4.09E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:-4.56|P-value:6.96E-6 ID:SGTC_117|Compound:lucigenin|FD-Score:3.58|P-value:3.17E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:5.87|P-value:1.11E-8 ID:SGTC_298|Compound:1391-0729|FD-Score:-4.00|P-value:6.85E-5 ID:SGTC_359|Compound:0081-0058|FD-Score:6.56|P-value:2.04E-10 ID:SGTC_426|Compound:0988-0037|FD-Score:3.59|P-value:3.12E-4 ID:SGTC_495|Compound:decylubiquinone|FD-Score:-3.59|P-value:3.14E-4 ID:SGTC_564|Compound:3555-0129|FD-Score:-3.78|P-value:1.62E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:3.63|P-value:2.66E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:3.26|P-value:9.45E-4 ID:SGTC_588|Compound:3970-1144|FD-Score:-4.16|P-value:3.78E-5 ID:SGTC_594|Compound:1487-1584|FD-Score:-5.31|P-value:2.11E-7 ID:SGTC_631|Compound:k072-0230|FD-Score:-3.74|P-value:1.84E-4 ID:SGTC_667|Compound:1498-1044|FD-Score:3.98|P-value:7.34E-5 ID:SGTC_760|Compound:0568-0725|FD-Score:3.92|P-value:9.25E-5 ID:SGTC_772|Compound:k072-0172|FD-Score:4.49|P-value:9.38E-6 ID:SGTC_816|Compound:0325-0417|FD-Score:4.82|P-value:2.20E-6 ID:SGTC_817|Compound:1391-0715|FD-Score:-3.87|P-value:1.15E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:-5.52|P-value:7.29E-8 ID:SGTC_824|Compound:1304-0008|FD-Score:4.01|P-value:6.67E-5 ID:SGTC_1082|Compound:camptothecin|FD-Score:7.84|P-value:3.84E-14 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:9.74|P-value:8.10E-21 ID:SGTC_1278|Compound:0874-0585|FD-Score:3.44|P-value:5.15E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:3.33|P-value:7.47E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:-4.45|P-value:1.13E-5 ID:SGTC_1707|Compound:st032323|FD-Score:4.09|P-value:4.89E-5 ID:SGTC_1775|Compound:st081588|FD-Score:5.36|P-value:1.63E-7 ID:SGTC_1842|Compound:st055362|FD-Score:3.43|P-value:5.47E-4 ID:SGTC_2023|Compound:5144124|FD-Score:3.40|P-value:5.97E-4 ID:SGTC_2359|Compound:9039622|FD-Score:4.75|P-value:2.94E-6 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:3.87|P-value:1.13E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:-3.98|P-value:7.41E-5 ID:SGTC_2589|Compound:lobaric acid|FD-Score:4.23|P-value:2.81E-5 ID:SGTC_3022|Compound:9087993|FD-Score:3.26|P-value:9.48E-4 ID:SGTC_3023|Compound:9089636|FD-Score:4.32|P-value:1.90E-5 ID:SGTC_3036|Compound:9090181|FD-Score:5.38|P-value:1.48E-7 ID:SGTC_3082|Compound:9117008|FD-Score:4.21|P-value:3.05E-5 ID:SGTC_3215|Compound:9129942|FD-Score:-3.74|P-value:1.86E-4 ID:SGTC_3299|Compound:9121982|FD-Score:4.87|P-value:1.70E-6

Top fitness defect scores for YPR066W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1083 didemethylchlorpromazine sulfoxide 9.74 707.4 nM 8.10E-21 redox potentiating 6 9.82E-23
2 SGTC_1082 camptothecin 7.84 424.9 nM 3.84E-14 DNA damage response 12 2.17E-15
3 SGTC_359 0081-0058 6.56 15.9 µM 2.04E-10 60S ribosome export 9 2.71E-11
4 SGTC_268 camptothecin 5.87 39.6 µM 1.11E-8 DNA damage response 32 2.18E-9
5 SGTC_3036 9090181 5.38 49.5 µM 1.48E-7 1 3.76E-8
6 SGTC_1775 st081588 5.36 35.2 µM 1.63E-7 60S ribosome export 8 4.16E-8
7 SGTC_3299 9121982 4.87 43.1 µM 1.70E-6 11 5.48E-7
8 SGTC_816 0325-0417 4.82 81.3 µM 2.20E-6 4 7.26E-7
9 SGTC_2359 9039622 4.75 200.0 µM 2.94E-6 endomembrane recycling 9 9.98E-7
10 SGTC_772 k072-0172 4.49 35.3 µM 9.38E-6 11 3.57E-6
11 SGTC_3023 9089636 4.32 49.5 µM 1.90E-5 3 7.73E-6
12 SGTC_2589 lobaric acid 4.23 100.0 µM 2.81E-5 21 1.19E-5
13 SGTC_3082 9117008 4.21 49.5 µM 3.05E-5 13 1.30E-5
14 SGTC_87 0100-0052 4.13 35.5 µM 4.09E-5 98 1.79E-5
15 SGTC_1707 st032323 4.09 6.3 µM 4.89E-5 DNA damage response 52 2.18E-5
16 SGTC_59 0330-0160 4.05 1.3 µM 5.66E-5 104 2.56E-5
17 SGTC_824 1304-0008 4.01 100.0 µM 6.67E-5 DNA damage response 40 3.07E-5
18 SGTC_667 1498-1044 3.98 59.0 µM 7.34E-5 RPP1 & pyrimidine depletion 17 3.40E-5
19 SGTC_760 0568-0725 3.92 82.9 µM 9.25E-5 10 4.39E-5
20 SGTC_36 0335-0881 3.88 14.2 µM 1.08E-4 ubiquinone biosynthesis & proteasome 123 5.20E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.260 6.84E-53 YPL251W_d YPL251W_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
0.249 1.05E-48 YBL088C TEL1 hom Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585)
0.225 9.70E-40 YPL252C YAH1 het Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.217 5.18E-37 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.215 1.46E-36 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.211 5.79E-35 YDL059C RAD59 hom Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
0.199 2.54E-31 YDR431W_d YDR431W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.168 1.27E-22 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.151 1.42E-18 YGL175C SAE2 hom Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents
0.149 3.81E-18 YDR076W RAD55 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.143 9.50E-17 YGL165C_d YGL165C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W
0.139 6.22E-16 YDR376W ARH1 het Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
0.137 1.79E-15 YLL012W YEH1 hom Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
0.124 4.83E-13 YDL049C KNH1 hom Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.119 4.70E-12 YDR430C CYM1 hom Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology