YPR067W / ISA2

Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations

Zygosity: Homozygous strain
fixedexpanded
Profile for YPR067W / ISA2

Click on Significant Values for Screen Details ID:SGTC_21|Compound:0849-0113|FD-Score:4.55|P-value:2.20E-5 ID:SGTC_23|Compound:0213-0011|FD-Score:4.50|P-value:2.66E-5 ID:SGTC_36|Compound:0335-0881|FD-Score:5.48|P-value:4.27E-7 ID:SGTC_54|Compound:0159-0055|FD-Score:3.97|P-value:1.87E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:4.31|P-value:5.48E-5 ID:SGTC_67|Compound:0335-0847|FD-Score:4.60|P-value:1.81E-5 ID:SGTC_84|Compound:0443-0269|FD-Score:4.78|P-value:8.79E-6 ID:SGTC_86|Compound:0417-1630|FD-Score:8.61|P-value:4.20E-15 ID:SGTC_91|Compound:0417-1751|FD-Score:4.26|P-value:6.70E-5 ID:SGTC_100|Compound:0086-0128|FD-Score:3.72|P-value:4.27E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:3.86|P-value:2.72E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:7.46|P-value:9.29E-12 ID:SGTC_134|Compound:0269-0018|FD-Score:6.67|P-value:9.60E-10 ID:SGTC_142|Compound:0335-0849|FD-Score:4.84|P-value:7.00E-6 ID:SGTC_158|Compound:k038-0087|FD-Score:3.56|P-value:7.11E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:3.74|P-value:3.99E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:6.83|P-value:3.97E-10 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:3.48|P-value:9.17E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:6.26|P-value:9.04E-9 ID:SGTC_635|Compound:k038-0028|FD-Score:3.94|P-value:2.06E-4 ID:SGTC_794|Compound:k038-0031|FD-Score:3.68|P-value:4.95E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:3.51|P-value:8.48E-4 ID:SGTC_868|Compound:0527-0199|FD-Score:3.82|P-value:3.06E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:3.68|P-value:4.95E-4 ID:SGTC_1020|Compound:4335-0961|FD-Score:4.33|P-value:5.16E-5 ID:SGTC_1094|Compound:duloxetine|FD-Score:4.07|P-value:1.30E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:5.01|P-value:3.38E-6 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.48|P-value:9.31E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:4.64|P-value:1.52E-5 ID:SGTC_1260|Compound:0700-1617|FD-Score:15.90|P-value:7.24E-47 ID:SGTC_1266|Compound:0828-0227|FD-Score:-4.13|P-value:8.03E-5 ID:SGTC_1352|Compound:1496-0991|FD-Score:3.90|P-value:2.33E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:7.19|P-value:4.70E-11 ID:SGTC_1445|Compound:4239-0156|FD-Score:-4.81|P-value:5.68E-6 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.94|P-value:1.58E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:4.17|P-value:9.24E-5 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:4.16|P-value:9.53E-5 ID:SGTC_1553|Compound:gossypol|FD-Score:3.68|P-value:4.93E-4 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:3.83|P-value:2.98E-4 ID:SGTC_1672|Compound:st016598|FD-Score:5.02|P-value:3.24E-6 ID:SGTC_1682|Compound:st012968|FD-Score:4.06|P-value:1.34E-4 ID:SGTC_1750|Compound:st081859|FD-Score:5.66|P-value:1.81E-7 ID:SGTC_1792|Compound:st045773|FD-Score:5.66|P-value:1.75E-7 ID:SGTC_1950|Compound:st077106|FD-Score:-4.59|P-value:1.36E-5 ID:SGTC_1986|Compound:st071812|FD-Score:3.75|P-value:3.93E-4 ID:SGTC_2159|Compound:5658611|FD-Score:3.56|P-value:7.26E-4 ID:SGTC_2220|Compound:6782180|FD-Score:-4.13|P-value:8.12E-5 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:8.63|P-value:3.66E-15 ID:SGTC_2250|Compound:alverine citrate|FD-Score:7.95|P-value:3.87E-13 ID:SGTC_2295|Compound:7971645|FD-Score:3.95|P-value:1.99E-4 ID:SGTC_2302|Compound:7771753|FD-Score:3.67|P-value:5.10E-4 ID:SGTC_2385|Compound:9074692|FD-Score:5.32|P-value:8.80E-7 ID:SGTC_2467|Compound:5474540|FD-Score:3.64|P-value:5.50E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:7.26|P-value:3.14E-11 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:-3.68|P-value:3.82E-4 ID:SGTC_2586|Compound:curcumin|FD-Score:-3.72|P-value:3.27E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:4.14|P-value:1.03E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:3.52|P-value:8.16E-4 ID:SGTC_2835|Compound:9006588|FD-Score:4.17|P-value:9.03E-5 ID:SGTC_2906|Compound:7926292|FD-Score:-4.37|P-value:3.32E-5 ID:SGTC_2916|Compound:7875993|FD-Score:3.85|P-value:2.83E-4 ID:SGTC_2940|Compound:9060638|FD-Score:3.97|P-value:1.84E-4 ID:SGTC_3036|Compound:9090181|FD-Score:4.58|P-value:1.97E-5 ID:SGTC_21|Compound:0849-0113|FD-Score:4.55|P-value:2.20E-5 ID:SGTC_23|Compound:0213-0011|FD-Score:4.50|P-value:2.66E-5 ID:SGTC_36|Compound:0335-0881|FD-Score:5.48|P-value:4.27E-7 ID:SGTC_54|Compound:0159-0055|FD-Score:3.97|P-value:1.87E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:4.31|P-value:5.48E-5 ID:SGTC_67|Compound:0335-0847|FD-Score:4.60|P-value:1.81E-5 ID:SGTC_84|Compound:0443-0269|FD-Score:4.78|P-value:8.79E-6 ID:SGTC_86|Compound:0417-1630|FD-Score:8.61|P-value:4.20E-15 ID:SGTC_91|Compound:0417-1751|FD-Score:4.26|P-value:6.70E-5 ID:SGTC_100|Compound:0086-0128|FD-Score:3.72|P-value:4.27E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:3.86|P-value:2.72E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:7.46|P-value:9.29E-12 ID:SGTC_134|Compound:0269-0018|FD-Score:6.67|P-value:9.60E-10 ID:SGTC_142|Compound:0335-0849|FD-Score:4.84|P-value:7.00E-6 ID:SGTC_158|Compound:k038-0087|FD-Score:3.56|P-value:7.11E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:3.74|P-value:3.99E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:6.83|P-value:3.97E-10 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:3.48|P-value:9.17E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:6.26|P-value:9.04E-9 ID:SGTC_635|Compound:k038-0028|FD-Score:3.94|P-value:2.06E-4 ID:SGTC_794|Compound:k038-0031|FD-Score:3.68|P-value:4.95E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:3.51|P-value:8.48E-4 ID:SGTC_868|Compound:0527-0199|FD-Score:3.82|P-value:3.06E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:3.68|P-value:4.95E-4 ID:SGTC_1020|Compound:4335-0961|FD-Score:4.33|P-value:5.16E-5 ID:SGTC_1094|Compound:duloxetine|FD-Score:4.07|P-value:1.30E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:5.01|P-value:3.38E-6 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.48|P-value:9.31E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:4.64|P-value:1.52E-5 ID:SGTC_1260|Compound:0700-1617|FD-Score:15.90|P-value:7.24E-47 ID:SGTC_1266|Compound:0828-0227|FD-Score:-4.13|P-value:8.03E-5 ID:SGTC_1352|Compound:1496-0991|FD-Score:3.90|P-value:2.33E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:7.19|P-value:4.70E-11 ID:SGTC_1445|Compound:4239-0156|FD-Score:-4.81|P-value:5.68E-6 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.94|P-value:1.58E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:4.17|P-value:9.24E-5 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:4.16|P-value:9.53E-5 ID:SGTC_1553|Compound:gossypol|FD-Score:3.68|P-value:4.93E-4 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:3.83|P-value:2.98E-4 ID:SGTC_1672|Compound:st016598|FD-Score:5.02|P-value:3.24E-6 ID:SGTC_1682|Compound:st012968|FD-Score:4.06|P-value:1.34E-4 ID:SGTC_1750|Compound:st081859|FD-Score:5.66|P-value:1.81E-7 ID:SGTC_1792|Compound:st045773|FD-Score:5.66|P-value:1.75E-7 ID:SGTC_1950|Compound:st077106|FD-Score:-4.59|P-value:1.36E-5 ID:SGTC_1986|Compound:st071812|FD-Score:3.75|P-value:3.93E-4 ID:SGTC_2159|Compound:5658611|FD-Score:3.56|P-value:7.26E-4 ID:SGTC_2220|Compound:6782180|FD-Score:-4.13|P-value:8.12E-5 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:8.63|P-value:3.66E-15 ID:SGTC_2250|Compound:alverine citrate|FD-Score:7.95|P-value:3.87E-13 ID:SGTC_2295|Compound:7971645|FD-Score:3.95|P-value:1.99E-4 ID:SGTC_2302|Compound:7771753|FD-Score:3.67|P-value:5.10E-4 ID:SGTC_2385|Compound:9074692|FD-Score:5.32|P-value:8.80E-7 ID:SGTC_2467|Compound:5474540|FD-Score:3.64|P-value:5.50E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:7.26|P-value:3.14E-11 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:-3.68|P-value:3.82E-4 ID:SGTC_2586|Compound:curcumin|FD-Score:-3.72|P-value:3.27E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:4.14|P-value:1.03E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:3.52|P-value:8.16E-4 ID:SGTC_2835|Compound:9006588|FD-Score:4.17|P-value:9.03E-5 ID:SGTC_2906|Compound:7926292|FD-Score:-4.37|P-value:3.32E-5 ID:SGTC_2916|Compound:7875993|FD-Score:3.85|P-value:2.83E-4 ID:SGTC_2940|Compound:9060638|FD-Score:3.97|P-value:1.84E-4 ID:SGTC_3036|Compound:9090181|FD-Score:4.58|P-value:1.97E-5

Top fitness defect scores for YPR067W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1260 0700-1617 15.90 7.8 µM 7.24E-47 calcium & mitochondrial duress 2 5.86E-57
2 SGTC_2232 chlorophetanol 8.63 41.5 µM 3.66E-15 TRP & mitochondrial translation 17 3.10E-18
3 SGTC_86 0417-1630 8.61 471.9 µM 4.20E-15 ubiquinone biosynthesis & proteasome 52 3.66E-18
4 SGTC_2250 alverine citrate 7.95 130.0 µM 3.87E-13 20 9.18E-16
5 SGTC_117 lucigenin 7.46 22.1 µM 9.29E-12 redox potentiating 24 4.45E-14
6 SGTC_2513 baicalein 7.26 25.6 µM 3.14E-11 calcium & mitochondrial duress 5 1.97E-13
7 SGTC_1380 2291-0730 7.19 164.0 µM 4.70E-11 1 3.22E-13
8 SGTC_236 1509-0011 6.83 5.3 µM 3.97E-10 271 4.37E-12
9 SGTC_134 0269-0018 6.67 6.9 µM 9.60E-10 72 1.28E-11
10 SGTC_275 trichlorophene 6.26 5.7 µM 9.04E-9 mitochondrial stress 109 1.98E-10
11 SGTC_1792 st045773 5.66 43.6 µM 1.75E-7 6 7.38E-9
12 SGTC_1750 st081859 5.66 2.4 µM 1.81E-7 endomembrane recycling 117 7.68E-9
13 SGTC_36 0335-0881 5.48 14.2 µM 4.27E-7 ubiquinone biosynthesis & proteasome 75 2.19E-8
14 SGTC_2385 9074692 5.32 200.0 µM 8.80E-7 17 5.29E-8
15 SGTC_1672 st016598 5.02 6.5 µM 3.24E-6 endomembrane recycling 41 2.59E-7
16 SGTC_1126 isoteolin 5.01 1.0 µM 3.38E-6 11 2.72E-7
17 SGTC_142 0335-0849 4.84 62.7 µM 7.00E-6 ubiquinone biosynthesis & proteasome 88 6.62E-7
18 SGTC_84 0443-0269 4.78 128.8 µM 8.79E-6 11 8.74E-7
19 SGTC_1255 0566-0045 4.64 221.0 µM 1.52E-5 excess fatty acid 16 1.71E-6
20 SGTC_67 0335-0847 4.60 266.0 µM 1.81E-5 ubiquinone biosynthesis & proteasome 92 2.11E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.333 2.00E-87 YDR204W COQ4 hom Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex
0.311 2.03E-76 YOR125C CAT5 hom Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation
0.311 4.75E-76 YJR122W IBA57 hom Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system
0.303 3.12E-72 YLL027W ISA1 hom Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins
0.302 1.05E-71 YNR041C COQ2 hom Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis
0.293 3.12E-67 YLR201C COQ9 hom Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes
0.278 2.02E-60 YLR304C ACO1 hom Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy
0.274 1.34E-58 YLR202C_d YLR202C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron
0.274 1.16E-58 YOL096C COQ3 hom O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein
0.268 2.61E-56 YGL119W COQ8 hom Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.254 1.64E-50 YBR179C FZO1 hom Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system
0.252 9.89E-50 YML110C COQ5 hom 2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes
0.251 1.82E-49 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.248 3.95E-48 YFL016C MDJ1 hom Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones
0.238 2.70E-44 YGR255C COQ6 hom Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)