YPR086W / SUA7

Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPR086W / SUA7

Click on Significant Values for Screen Details ID:SGTC_27|Compound:0485-0001|FD-Score:-3.11|P-value:9.57E-4 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:3.36|P-value:5.08E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:3.43|P-value:3.89E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:3.62|P-value:2.00E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:-4.10|P-value:2.21E-5 ID:SGTC_260|Compound:nsc-65238|FD-Score:3.18|P-value:9.49E-4 ID:SGTC_480|Compound:am 92016|FD-Score:4.24|P-value:1.65E-5 ID:SGTC_559|Compound:1550-0661|FD-Score:-3.52|P-value:2.25E-4 ID:SGTC_763|Compound:3453-1109|FD-Score:-3.69|P-value:1.18E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.24|P-value:7.74E-4 ID:SGTC_1788|Compound:fluconazole|FD-Score:-3.94|P-value:4.37E-5 ID:SGTC_1981|Compound:st071826|FD-Score:3.89|P-value:6.89E-5 ID:SGTC_2151|Compound:5563267|FD-Score:9.47|P-value:5.43E-21 ID:SGTC_2152|Compound:5568786|FD-Score:3.99|P-value:4.58E-5 ID:SGTC_2154|Compound:5570129|FD-Score:3.37|P-value:4.88E-4 ID:SGTC_2288|Compound:7946289|FD-Score:-3.43|P-value:3.12E-4 ID:SGTC_2394|Compound:5221648|FD-Score:3.39|P-value:4.57E-4 ID:SGTC_2404|Compound:5161160|FD-Score:3.23|P-value:7.94E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:4.92|P-value:6.92E-7 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.54|P-value:2.62E-4 ID:SGTC_2601|Compound:securinine|FD-Score:3.83|P-value:8.65E-5 ID:SGTC_2694|Compound:st077685|FD-Score:5.53|P-value:2.73E-8 ID:SGTC_2935|Compound:9038016|FD-Score:-3.13|P-value:8.89E-4 ID:SGTC_2989|Compound:9073649|FD-Score:3.81|P-value:9.35E-5 ID:SGTC_2993|Compound:9054257|FD-Score:3.33|P-value:5.55E-4 ID:SGTC_2999|Compound:9069765|FD-Score:-3.63|P-value:1.49E-4 ID:SGTC_3044|Compound:9093193|FD-Score:3.55|P-value:2.52E-4 ID:SGTC_27|Compound:0485-0001|FD-Score:-3.11|P-value:9.57E-4 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:3.36|P-value:5.08E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:3.43|P-value:3.89E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:3.62|P-value:2.00E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:-4.10|P-value:2.21E-5 ID:SGTC_260|Compound:nsc-65238|FD-Score:3.18|P-value:9.49E-4 ID:SGTC_480|Compound:am 92016|FD-Score:4.24|P-value:1.65E-5 ID:SGTC_559|Compound:1550-0661|FD-Score:-3.52|P-value:2.25E-4 ID:SGTC_763|Compound:3453-1109|FD-Score:-3.69|P-value:1.18E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.24|P-value:7.74E-4 ID:SGTC_1788|Compound:fluconazole|FD-Score:-3.94|P-value:4.37E-5 ID:SGTC_1981|Compound:st071826|FD-Score:3.89|P-value:6.89E-5 ID:SGTC_2151|Compound:5563267|FD-Score:9.47|P-value:5.43E-21 ID:SGTC_2152|Compound:5568786|FD-Score:3.99|P-value:4.58E-5 ID:SGTC_2154|Compound:5570129|FD-Score:3.37|P-value:4.88E-4 ID:SGTC_2288|Compound:7946289|FD-Score:-3.43|P-value:3.12E-4 ID:SGTC_2394|Compound:5221648|FD-Score:3.39|P-value:4.57E-4 ID:SGTC_2404|Compound:5161160|FD-Score:3.23|P-value:7.94E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:4.92|P-value:6.92E-7 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.54|P-value:2.62E-4 ID:SGTC_2601|Compound:securinine|FD-Score:3.83|P-value:8.65E-5 ID:SGTC_2694|Compound:st077685|FD-Score:5.53|P-value:2.73E-8 ID:SGTC_2935|Compound:9038016|FD-Score:-3.13|P-value:8.89E-4 ID:SGTC_2989|Compound:9073649|FD-Score:3.81|P-value:9.35E-5 ID:SGTC_2993|Compound:9054257|FD-Score:3.33|P-value:5.55E-4 ID:SGTC_2999|Compound:9069765|FD-Score:-3.63|P-value:1.49E-4 ID:SGTC_3044|Compound:9093193|FD-Score:3.55|P-value:2.52E-4

Top fitness defect scores for YPR086W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2151 5563267 9.47 85.4 µM 5.43E-21 5 1.35E-21 0.10
2 SGTC_2694 st077685 5.53 81.9 µM 2.73E-8 endomembrane recycling 3 1.56E-8 0.46
3 SGTC_2409 alverine citrate 4.92 64.0 µM 6.92E-7 9 4.34E-7 0.38
4 SGTC_480 am 92016 4.24 50.0 µM 1.65E-5 2 1.14E-5 0.26
5 SGTC_2152 5568786 3.99 200.0 µM 4.58E-5 4 3.27E-5 0.41
6 SGTC_1981 st071826 3.89 47.0 µM 6.89E-5 5 4.98E-5 0.05
7 SGTC_2601 securinine 3.83 100.0 µM 8.65E-5 20 6.29E-5 0.03
8 SGTC_2989 9073649 3.81 71.4 µM 9.35E-5 9 6.82E-5 0.00
9 SGTC_149 3970-0822 3.62 46.1 µM 2.00E-4 tubulin folding & SWR complex 15 1.49E-4 0.02
10 SGTC_3044 9093193 3.55 49.5 µM 2.52E-4 10 1.90E-4 0.17
11 SGTC_2419 paclitaxel 3.54 166.5 µM 2.62E-4 6 1.97E-4 0.08
12 SGTC_123 0083-0120 3.43 66.7 µM 3.89E-4 16 2.97E-4 0.06
13 SGTC_2394 5221648 3.39 200.0 µM 4.57E-4 11 3.50E-4 0.08
14 SGTC_2154 5570129 3.37 200.0 µM 4.88E-4 4 3.75E-4 0.01
15 SGTC_28 pentachloropyridine N-oxide 3.36 1.6 µM 5.08E-4 TSC3-RPN4 6 3.91E-4 0.44
16 SGTC_2993 9054257 3.33 71.4 µM 5.55E-4 fatty acid desaturase (OLE1) 47 4.28E-4 0.04
17 SGTC_915 methyl methanesulfonate (MMS) 3.24 110.0 mM 7.74E-4 DNA damage response 17 6.03E-4 0.02
18 SGTC_2404 5161160 3.23 200.0 µM 7.94E-4 15 6.19E-4 0.04
19 SGTC_260 nsc-65238 3.18 4.0 µM 9.49E-4 endomembrane recycling 9 7.45E-4 0.11
20 SGTC_1241 0364-0022 3.12 50.8 µM 0.00114 ERG2 9 8.97E-4 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.146 1.97E-17 YPR085C ASA1 het Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
0.139 7.78E-16 YDR212W TCP1 het Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
0.130 3.61E-14 YGR204W ADE3 hom Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine
0.124 6.02E-13 YMR174C PAI3 hom Cytoplasmic proteinase A (Pep4p) inhibitor, dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact
0.111 1.30E-10 YLR400W_d YLR400W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.107 4.52E-10 YFL042C_p YFL042C_p hom Putative protein of unknown function; YFL042C is not an essential gene
0.105 9.78E-10 YGR043C NQM1 hom Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift
0.104 1.75E-9 YPL249C GYP5 hom GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.103 2.66E-9 YDR302W GPI11 het ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog
0.098 1.29E-8 YGR049W SCM4 hom Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation
0.096 2.39E-8 YJL100W LSB6 hom Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization
0.096 2.56E-8 YJR046W TAH11 het DNA replication licensing factor, required for pre-replication complex assembly
0.095 3.54E-8 YLR197W NOP56 het Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
0.094 5.32E-8 YLR387C REH1 hom Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains
0.094 5.52E-8 YJL009W_d YJL009W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex