YPR094W / RDS3

Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPR094W / RDS3

Click on Significant Values for Screen Details ID:SGTC_7|Compound:0192-1582|FD-Score:3.14|P-value:8.08E-4 ID:SGTC_41|Compound:0478-0632|FD-Score:4.16|P-value:1.41E-5 ID:SGTC_49|Compound:0717-0965|FD-Score:3.78|P-value:7.24E-5 ID:SGTC_81|Compound:0411-0046|FD-Score:8.34|P-value:1.73E-17 ID:SGTC_153|Compound:4077-0274|FD-Score:-3.04|P-value:8.93E-4 ID:SGTC_188|Compound:3229-0966|FD-Score:3.50|P-value:2.18E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:3.24|P-value:5.75E-4 ID:SGTC_438|Compound:sphingosine|FD-Score:3.89|P-value:4.67E-5 ID:SGTC_441|Compound:propafenone|FD-Score:3.69|P-value:1.06E-4 ID:SGTC_444|Compound:curcumin|FD-Score:4.54|P-value:2.38E-6 ID:SGTC_461|Compound:N-acetylfarnesylcysteine|FD-Score:3.29|P-value:4.78E-4 ID:SGTC_551|Compound:k048-0028|FD-Score:-3.24|P-value:4.37E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:6.21|P-value:1.85E-10 ID:SGTC_812|Compound:4251-0442|FD-Score:3.25|P-value:5.50E-4 ID:SGTC_856|Compound:0433-0016|FD-Score:5.96|P-value:9.11E-10 ID:SGTC_875|Compound:0709-0210|FD-Score:3.28|P-value:4.97E-4 ID:SGTC_979|Compound:1426-2828|FD-Score:3.30|P-value:4.63E-4 ID:SGTC_1029|Compound:k015-0030|FD-Score:3.29|P-value:4.84E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:-3.89|P-value:3.42E-5 ID:SGTC_1160|Compound:4092-0570|FD-Score:4.04|P-value:2.42E-5 ID:SGTC_1193|Compound:1488-0126|FD-Score:3.13|P-value:8.42E-4 ID:SGTC_1211|Compound:1056-0033|FD-Score:3.25|P-value:5.59E-4 ID:SGTC_1382|Compound:3002-2117|FD-Score:3.29|P-value:4.71E-4 ID:SGTC_1646|Compound:st011709|FD-Score:3.84|P-value:5.74E-5 ID:SGTC_1744|Compound:st037937|FD-Score:4.51|P-value:2.81E-6 ID:SGTC_1771|Compound:st048882|FD-Score:5.77|P-value:2.99E-9 ID:SGTC_1774|Compound:st048371|FD-Score:3.65|P-value:1.25E-4 ID:SGTC_1783|Compound:st039459|FD-Score:-3.12|P-value:6.72E-4 ID:SGTC_1786|Compound:berberine|FD-Score:4.13|P-value:1.60E-5 ID:SGTC_1788|Compound:fluconazole|FD-Score:5.39|P-value:2.81E-8 ID:SGTC_1840|Compound:st055363|FD-Score:3.22|P-value:6.18E-4 ID:SGTC_1881|Compound:5652484|FD-Score:-3.33|P-value:3.15E-4 ID:SGTC_1913|Compound:st060213|FD-Score:3.70|P-value:1.00E-4 ID:SGTC_1930|Compound:st074634|FD-Score:3.90|P-value:4.40E-5 ID:SGTC_2065|Compound:5221596|FD-Score:3.65|P-value:1.20E-4 ID:SGTC_2226|Compound:7298446|FD-Score:3.59|P-value:1.53E-4 ID:SGTC_2260|Compound:7964312|FD-Score:-3.21|P-value:4.98E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.39|P-value:3.30E-4 ID:SGTC_2324|Compound:6149169|FD-Score:3.22|P-value:6.12E-4 ID:SGTC_2348|Compound:9022543|FD-Score:3.48|P-value:2.38E-4 ID:SGTC_2401|Compound:5128683|FD-Score:3.39|P-value:3.30E-4 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:4.20|P-value:1.20E-5 ID:SGTC_2523|Compound:spermine|FD-Score:3.47|P-value:2.43E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:3.76|P-value:7.75E-5 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:-3.24|P-value:4.36E-4 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:-3.06|P-value:8.44E-4 ID:SGTC_2926|Compound:9010726|FD-Score:3.89|P-value:4.50E-5 ID:SGTC_2965|Compound:9082772|FD-Score:-5.12|P-value:8.29E-8 ID:SGTC_2991|Compound:9074669|FD-Score:3.25|P-value:5.55E-4 ID:SGTC_3201|Compound:9113999|FD-Score:-3.54|P-value:1.41E-4 ID:SGTC_3204|Compound:9114183|FD-Score:6.12|P-value:3.27E-10 ID:SGTC_3207|Compound:9114163|FD-Score:3.18|P-value:7.09E-4 ID:SGTC_7|Compound:0192-1582|FD-Score:3.14|P-value:8.08E-4 ID:SGTC_41|Compound:0478-0632|FD-Score:4.16|P-value:1.41E-5 ID:SGTC_49|Compound:0717-0965|FD-Score:3.78|P-value:7.24E-5 ID:SGTC_81|Compound:0411-0046|FD-Score:8.34|P-value:1.73E-17 ID:SGTC_153|Compound:4077-0274|FD-Score:-3.04|P-value:8.93E-4 ID:SGTC_188|Compound:3229-0966|FD-Score:3.50|P-value:2.18E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:3.24|P-value:5.75E-4 ID:SGTC_438|Compound:sphingosine|FD-Score:3.89|P-value:4.67E-5 ID:SGTC_441|Compound:propafenone|FD-Score:3.69|P-value:1.06E-4 ID:SGTC_444|Compound:curcumin|FD-Score:4.54|P-value:2.38E-6 ID:SGTC_461|Compound:N-acetylfarnesylcysteine|FD-Score:3.29|P-value:4.78E-4 ID:SGTC_551|Compound:k048-0028|FD-Score:-3.24|P-value:4.37E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:6.21|P-value:1.85E-10 ID:SGTC_812|Compound:4251-0442|FD-Score:3.25|P-value:5.50E-4 ID:SGTC_856|Compound:0433-0016|FD-Score:5.96|P-value:9.11E-10 ID:SGTC_875|Compound:0709-0210|FD-Score:3.28|P-value:4.97E-4 ID:SGTC_979|Compound:1426-2828|FD-Score:3.30|P-value:4.63E-4 ID:SGTC_1029|Compound:k015-0030|FD-Score:3.29|P-value:4.84E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:-3.89|P-value:3.42E-5 ID:SGTC_1160|Compound:4092-0570|FD-Score:4.04|P-value:2.42E-5 ID:SGTC_1193|Compound:1488-0126|FD-Score:3.13|P-value:8.42E-4 ID:SGTC_1211|Compound:1056-0033|FD-Score:3.25|P-value:5.59E-4 ID:SGTC_1382|Compound:3002-2117|FD-Score:3.29|P-value:4.71E-4 ID:SGTC_1646|Compound:st011709|FD-Score:3.84|P-value:5.74E-5 ID:SGTC_1744|Compound:st037937|FD-Score:4.51|P-value:2.81E-6 ID:SGTC_1771|Compound:st048882|FD-Score:5.77|P-value:2.99E-9 ID:SGTC_1774|Compound:st048371|FD-Score:3.65|P-value:1.25E-4 ID:SGTC_1783|Compound:st039459|FD-Score:-3.12|P-value:6.72E-4 ID:SGTC_1786|Compound:berberine|FD-Score:4.13|P-value:1.60E-5 ID:SGTC_1788|Compound:fluconazole|FD-Score:5.39|P-value:2.81E-8 ID:SGTC_1840|Compound:st055363|FD-Score:3.22|P-value:6.18E-4 ID:SGTC_1881|Compound:5652484|FD-Score:-3.33|P-value:3.15E-4 ID:SGTC_1913|Compound:st060213|FD-Score:3.70|P-value:1.00E-4 ID:SGTC_1930|Compound:st074634|FD-Score:3.90|P-value:4.40E-5 ID:SGTC_2065|Compound:5221596|FD-Score:3.65|P-value:1.20E-4 ID:SGTC_2226|Compound:7298446|FD-Score:3.59|P-value:1.53E-4 ID:SGTC_2260|Compound:7964312|FD-Score:-3.21|P-value:4.98E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.39|P-value:3.30E-4 ID:SGTC_2324|Compound:6149169|FD-Score:3.22|P-value:6.12E-4 ID:SGTC_2348|Compound:9022543|FD-Score:3.48|P-value:2.38E-4 ID:SGTC_2401|Compound:5128683|FD-Score:3.39|P-value:3.30E-4 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:4.20|P-value:1.20E-5 ID:SGTC_2523|Compound:spermine|FD-Score:3.47|P-value:2.43E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:3.76|P-value:7.75E-5 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:-3.24|P-value:4.36E-4 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:-3.06|P-value:8.44E-4 ID:SGTC_2926|Compound:9010726|FD-Score:3.89|P-value:4.50E-5 ID:SGTC_2965|Compound:9082772|FD-Score:-5.12|P-value:8.29E-8 ID:SGTC_2991|Compound:9074669|FD-Score:3.25|P-value:5.55E-4 ID:SGTC_3201|Compound:9113999|FD-Score:-3.54|P-value:1.41E-4 ID:SGTC_3204|Compound:9114183|FD-Score:6.12|P-value:3.27E-10 ID:SGTC_3207|Compound:9114163|FD-Score:3.18|P-value:7.09E-4

Top fitness defect scores for YPR094W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_81 0411-0046 8.34 10.0 µM 1.73E-17 12 3.61E-17 0.07
2 SGTC_562 3772-2733 6.21 112.0 µM 1.85E-10 ERG2 4 2.63E-10 0.07
3 SGTC_3204 9114183 6.12 49.5 µM 3.27E-10 2 4.59E-10 1.59
4 SGTC_856 0433-0016 5.96 11.3 µM 9.11E-10 1 1.25E-9 1.23
5 SGTC_1771 st048882 5.77 4.2 µM 2.99E-9 RPP1 & pyrimidine depletion 5 4.00E-9 1.47
6 SGTC_1788 fluconazole 5.39 20.0 µM 2.81E-8 azole & statin 4 3.58E-8 0.34
7 SGTC_444 curcumin 4.54 90.4 µM 2.38E-6 iron homeostasis 8 2.76E-6 0.07
8 SGTC_1744 st037937 4.51 38.9 µM 2.81E-6 4 3.24E-6 0.40
9 SGTC_2496 diffractaic acid 4.20 79.8 µM 1.20E-5 5 1.35E-5 0.26
10 SGTC_41 0478-0632 4.16 82.6 µM 1.41E-5 12 1.57E-5 0.28
11 SGTC_1786 berberine 4.13 21.6 µM 1.60E-5 60S ribosome export 15 1.78E-5 0.02
12 SGTC_1160 4092-0570 4.04 10.5 µM 2.42E-5 plasma membrane duress 11 2.67E-5 0.39
13 SGTC_1930 st074634 3.90 34.3 µM 4.40E-5 RNA pol III & RNase P/MRP 15 4.81E-5 0.00
14 SGTC_2926 9010726 3.89 65.2 µM 4.50E-5 9 4.91E-5 0.09
15 SGTC_438 sphingosine 3.89 6.7 µM 4.67E-5 2 5.09E-5 0.35
16 SGTC_1646 st011709 3.84 75.6 µM 5.74E-5 7 6.24E-5 0.35
17 SGTC_49 0717-0965 3.78 32.1 µM 7.24E-5 60S ribosome export 10 7.83E-5 0.07
18 SGTC_2604 4'-hydroxychalcone 3.76 11.3 µM 7.75E-5 17 8.37E-5 0.02
19 SGTC_1913 st060213 3.70 96.1 µM 1.00E-4 4 1.08E-4 0.31
20 SGTC_441 propafenone 3.69 146.0 µM 1.06E-4 6 1.13E-4 0.46

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.182 2.03E-26 YDR272W GLO2 hom Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate
0.130 4.10E-14 YDL140C RPO21 het RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
0.124 5.86E-13 YDR189W SLY1 het Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex
0.119 5.17E-12 YDR041W RSM10 het Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins
0.109 2.16E-10 YDR294C DPL1 hom Dihydrosphingosine phosphate lyase, regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate
0.108 3.37E-10 YNL224C SQS1 hom Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress
0.098 1.48E-8 YMR260C TIF11 het Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3
0.096 2.46E-8 YDR416W SYF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans
0.091 1.29E-7 YJR088C EMC2 hom Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35
0.085 7.89E-7 YGR269W_d YGR269W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C
0.085 8.26E-7 YHR167W THP2 hom Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance
0.080 3.69E-6 YDR478W SNM1 het Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
0.078 5.92E-6 YJR087W_d YJR087W_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and ECM2
0.077 7.18E-6 YOL146W PSF3 het Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
0.077 7.38E-6 YKL009W MRT4 hom Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus