YPR103W / PRE2

Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPR103W / PRE2

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:5.13|P-value:4.99E-7 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.45|P-value:5.50E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:3.41|P-value:5.71E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:7.24|P-value:2.74E-12 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.56|P-value:3.91E-4 ID:SGTC_187|Compound:1488-2104|FD-Score:4.01|P-value:6.46E-5 ID:SGTC_189|Compound:3970-0742|FD-Score:3.87|P-value:1.13E-4 ID:SGTC_201|Compound:4262-0159|FD-Score:4.51|P-value:8.57E-6 ID:SGTC_374|Compound:0880-0231|FD-Score:5.00|P-value:9.49E-7 ID:SGTC_383|Compound:2324-0395|FD-Score:-3.69|P-value:2.47E-4 ID:SGTC_444|Compound:curcumin|FD-Score:5.40|P-value:1.35E-7 ID:SGTC_450|Compound:aminopyrene|FD-Score:4.68|P-value:4.14E-6 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:3.90|P-value:9.96E-5 ID:SGTC_547|Compound:1120-0019|FD-Score:-4.00|P-value:7.87E-5 ID:SGTC_673|Compound:1082-0474|FD-Score:3.46|P-value:4.84E-4 ID:SGTC_686|Compound:1319-0111|FD-Score:4.82|P-value:2.12E-6 ID:SGTC_689|Compound:0833-0039|FD-Score:3.41|P-value:5.66E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-4.28|P-value:2.61E-5 ID:SGTC_864|Compound:0469-0573|FD-Score:4.10|P-value:4.68E-5 ID:SGTC_878|Compound:0828-0289|FD-Score:4.99|P-value:1.00E-6 ID:SGTC_879|Compound:0831-1030|FD-Score:5.31|P-value:2.13E-7 ID:SGTC_902|Compound:1120-0002|FD-Score:-4.40|P-value:1.58E-5 ID:SGTC_933|Compound:2914-0560|FD-Score:3.24|P-value:9.98E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:4.90|P-value:1.51E-6 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.34|P-value:7.99E-4 ID:SGTC_1171|Compound:0368-0077|FD-Score:-3.44|P-value:5.75E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:-3.31|P-value:8.97E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-4.57|P-value:7.75E-6 ID:SGTC_1353|Compound:1498-1206|FD-Score:-3.29|P-value:9.58E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:-3.43|P-value:5.97E-4 ID:SGTC_1430|Compound:4049-0248|FD-Score:3.80|P-value:1.46E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.79|P-value:2.46E-6 ID:SGTC_1610|Compound:st001799|FD-Score:-3.95|P-value:9.28E-5 ID:SGTC_1614|Compound:st002383|FD-Score:-4.43|P-value:1.38E-5 ID:SGTC_1615|Compound:st002656|FD-Score:-3.51|P-value:4.60E-4 ID:SGTC_1689|Compound:st020265|FD-Score:3.61|P-value:2.89E-4 ID:SGTC_1728|Compound:st035614|FD-Score:-8.92|P-value:1.36E-17 ID:SGTC_1729|Compound:st035615|FD-Score:-5.00|P-value:1.14E-6 ID:SGTC_1730|Compound:st033808|FD-Score:-4.47|P-value:1.16E-5 ID:SGTC_1732|Compound:st034308|FD-Score:-5.69|P-value:3.52E-8 ID:SGTC_1759|Compound:st043059|FD-Score:4.12|P-value:4.25E-5 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-4.34|P-value:2.05E-5 ID:SGTC_1787|Compound:fluconazole|FD-Score:-4.24|P-value:3.01E-5 ID:SGTC_1829|Compound:berberine|FD-Score:3.67|P-value:2.28E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:7.38|P-value:1.04E-12 ID:SGTC_1845|Compound:st056753|FD-Score:3.53|P-value:3.79E-4 ID:SGTC_1853|Compound:st056710|FD-Score:3.41|P-value:5.64E-4 ID:SGTC_1859|Compound:st055998|FD-Score:3.32|P-value:7.69E-4 ID:SGTC_2050|Compound:5263056|FD-Score:-4.10|P-value:5.33E-5 ID:SGTC_2054|Compound:5233173|FD-Score:-5.58|P-value:6.53E-8 ID:SGTC_2055|Compound:5235184|FD-Score:3.49|P-value:4.34E-4 ID:SGTC_2129|Compound:girgensonine|FD-Score:-3.93|P-value:1.02E-4 ID:SGTC_2132|Compound:5317594|FD-Score:4.14|P-value:3.86E-5 ID:SGTC_2158|Compound:5657123|FD-Score:4.62|P-value:5.39E-6 ID:SGTC_2161|Compound:5662504|FD-Score:-3.74|P-value:2.01E-4 ID:SGTC_2167|Compound:5876490|FD-Score:-3.67|P-value:2.60E-4 ID:SGTC_2183|Compound:6420625|FD-Score:-3.56|P-value:3.83E-4 ID:SGTC_2211|Compound:7117706|FD-Score:3.69|P-value:2.15E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:3.28|P-value:8.75E-4 ID:SGTC_2233|Compound:sulbentine|FD-Score:-4.14|P-value:4.55E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:5.69|P-value:2.89E-8 ID:SGTC_2254|Compound:7938975|FD-Score:3.59|P-value:3.10E-4 ID:SGTC_2275|Compound:7947463|FD-Score:4.16|P-value:3.64E-5 ID:SGTC_2414|Compound:st077232|FD-Score:5.00|P-value:9.48E-7 ID:SGTC_2415|Compound:st077333|FD-Score:5.21|P-value:3.43E-7 ID:SGTC_2418|Compound:pentamidine|FD-Score:-3.70|P-value:2.35E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.69|P-value:2.15E-4 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:3.24|P-value:9.89E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:5.24|P-value:2.97E-7 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.90|P-value:9.99E-5 ID:SGTC_2846|Compound:9008642|FD-Score:3.33|P-value:7.44E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-4.40|P-value:1.60E-5 ID:SGTC_2931|Compound:9014674|FD-Score:3.64|P-value:2.57E-4 ID:SGTC_2941|Compound:9056145|FD-Score:-3.45|P-value:5.58E-4 ID:SGTC_2949|Compound:9072542|FD-Score:3.53|P-value:3.70E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-4.12|P-value:4.98E-5 ID:SGTC_2970|Compound:9090938|FD-Score:-3.60|P-value:3.38E-4 ID:SGTC_2979|Compound:9012552|FD-Score:3.32|P-value:7.58E-4 ID:SGTC_2984|Compound:9022109|FD-Score:3.57|P-value:3.26E-4 ID:SGTC_3027|Compound:9089541|FD-Score:-5.29|P-value:2.78E-7 ID:SGTC_3040|Compound:9091894|FD-Score:-6.11|P-value:3.62E-9 ID:SGTC_3062|Compound:9077494|FD-Score:-5.54|P-value:7.68E-8 ID:SGTC_3142|Compound:9086498|FD-Score:3.42|P-value:5.39E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-3.64|P-value:2.89E-4 ID:SGTC_3210|Compound:9128157|FD-Score:4.32|P-value:1.92E-5 ID:SGTC_3283|Compound:9109332|FD-Score:-6.06|P-value:4.75E-9 ID:SGTC_3298|Compound:9116858|FD-Score:3.62|P-value:2.79E-4 ID:SGTC_3303|Compound:9125618|FD-Score:-3.30|P-value:9.01E-4 ID:SGTC_3304|Compound:9125678|FD-Score:-4.08|P-value:5.69E-5 ID:SGTC_3331|Compound:9141060|FD-Score:3.43|P-value:5.27E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:5.13|P-value:4.99E-7 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.45|P-value:5.50E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:3.41|P-value:5.71E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:7.24|P-value:2.74E-12 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.56|P-value:3.91E-4 ID:SGTC_187|Compound:1488-2104|FD-Score:4.01|P-value:6.46E-5 ID:SGTC_189|Compound:3970-0742|FD-Score:3.87|P-value:1.13E-4 ID:SGTC_201|Compound:4262-0159|FD-Score:4.51|P-value:8.57E-6 ID:SGTC_374|Compound:0880-0231|FD-Score:5.00|P-value:9.49E-7 ID:SGTC_383|Compound:2324-0395|FD-Score:-3.69|P-value:2.47E-4 ID:SGTC_444|Compound:curcumin|FD-Score:5.40|P-value:1.35E-7 ID:SGTC_450|Compound:aminopyrene|FD-Score:4.68|P-value:4.14E-6 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:3.90|P-value:9.96E-5 ID:SGTC_547|Compound:1120-0019|FD-Score:-4.00|P-value:7.87E-5 ID:SGTC_673|Compound:1082-0474|FD-Score:3.46|P-value:4.84E-4 ID:SGTC_686|Compound:1319-0111|FD-Score:4.82|P-value:2.12E-6 ID:SGTC_689|Compound:0833-0039|FD-Score:3.41|P-value:5.66E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-4.28|P-value:2.61E-5 ID:SGTC_864|Compound:0469-0573|FD-Score:4.10|P-value:4.68E-5 ID:SGTC_878|Compound:0828-0289|FD-Score:4.99|P-value:1.00E-6 ID:SGTC_879|Compound:0831-1030|FD-Score:5.31|P-value:2.13E-7 ID:SGTC_902|Compound:1120-0002|FD-Score:-4.40|P-value:1.58E-5 ID:SGTC_933|Compound:2914-0560|FD-Score:3.24|P-value:9.98E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:4.90|P-value:1.51E-6 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.34|P-value:7.99E-4 ID:SGTC_1171|Compound:0368-0077|FD-Score:-3.44|P-value:5.75E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:-3.31|P-value:8.97E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-4.57|P-value:7.75E-6 ID:SGTC_1353|Compound:1498-1206|FD-Score:-3.29|P-value:9.58E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:-3.43|P-value:5.97E-4 ID:SGTC_1430|Compound:4049-0248|FD-Score:3.80|P-value:1.46E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.79|P-value:2.46E-6 ID:SGTC_1610|Compound:st001799|FD-Score:-3.95|P-value:9.28E-5 ID:SGTC_1614|Compound:st002383|FD-Score:-4.43|P-value:1.38E-5 ID:SGTC_1615|Compound:st002656|FD-Score:-3.51|P-value:4.60E-4 ID:SGTC_1689|Compound:st020265|FD-Score:3.61|P-value:2.89E-4 ID:SGTC_1728|Compound:st035614|FD-Score:-8.92|P-value:1.36E-17 ID:SGTC_1729|Compound:st035615|FD-Score:-5.00|P-value:1.14E-6 ID:SGTC_1730|Compound:st033808|FD-Score:-4.47|P-value:1.16E-5 ID:SGTC_1732|Compound:st034308|FD-Score:-5.69|P-value:3.52E-8 ID:SGTC_1759|Compound:st043059|FD-Score:4.12|P-value:4.25E-5 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-4.34|P-value:2.05E-5 ID:SGTC_1787|Compound:fluconazole|FD-Score:-4.24|P-value:3.01E-5 ID:SGTC_1829|Compound:berberine|FD-Score:3.67|P-value:2.28E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:7.38|P-value:1.04E-12 ID:SGTC_1845|Compound:st056753|FD-Score:3.53|P-value:3.79E-4 ID:SGTC_1853|Compound:st056710|FD-Score:3.41|P-value:5.64E-4 ID:SGTC_1859|Compound:st055998|FD-Score:3.32|P-value:7.69E-4 ID:SGTC_2050|Compound:5263056|FD-Score:-4.10|P-value:5.33E-5 ID:SGTC_2054|Compound:5233173|FD-Score:-5.58|P-value:6.53E-8 ID:SGTC_2055|Compound:5235184|FD-Score:3.49|P-value:4.34E-4 ID:SGTC_2129|Compound:girgensonine|FD-Score:-3.93|P-value:1.02E-4 ID:SGTC_2132|Compound:5317594|FD-Score:4.14|P-value:3.86E-5 ID:SGTC_2158|Compound:5657123|FD-Score:4.62|P-value:5.39E-6 ID:SGTC_2161|Compound:5662504|FD-Score:-3.74|P-value:2.01E-4 ID:SGTC_2167|Compound:5876490|FD-Score:-3.67|P-value:2.60E-4 ID:SGTC_2183|Compound:6420625|FD-Score:-3.56|P-value:3.83E-4 ID:SGTC_2211|Compound:7117706|FD-Score:3.69|P-value:2.15E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:3.28|P-value:8.75E-4 ID:SGTC_2233|Compound:sulbentine|FD-Score:-4.14|P-value:4.55E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:5.69|P-value:2.89E-8 ID:SGTC_2254|Compound:7938975|FD-Score:3.59|P-value:3.10E-4 ID:SGTC_2275|Compound:7947463|FD-Score:4.16|P-value:3.64E-5 ID:SGTC_2414|Compound:st077232|FD-Score:5.00|P-value:9.48E-7 ID:SGTC_2415|Compound:st077333|FD-Score:5.21|P-value:3.43E-7 ID:SGTC_2418|Compound:pentamidine|FD-Score:-3.70|P-value:2.35E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.69|P-value:2.15E-4 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:3.24|P-value:9.89E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:5.24|P-value:2.97E-7 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.90|P-value:9.99E-5 ID:SGTC_2846|Compound:9008642|FD-Score:3.33|P-value:7.44E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-4.40|P-value:1.60E-5 ID:SGTC_2931|Compound:9014674|FD-Score:3.64|P-value:2.57E-4 ID:SGTC_2941|Compound:9056145|FD-Score:-3.45|P-value:5.58E-4 ID:SGTC_2949|Compound:9072542|FD-Score:3.53|P-value:3.70E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-4.12|P-value:4.98E-5 ID:SGTC_2970|Compound:9090938|FD-Score:-3.60|P-value:3.38E-4 ID:SGTC_2979|Compound:9012552|FD-Score:3.32|P-value:7.58E-4 ID:SGTC_2984|Compound:9022109|FD-Score:3.57|P-value:3.26E-4 ID:SGTC_3027|Compound:9089541|FD-Score:-5.29|P-value:2.78E-7 ID:SGTC_3040|Compound:9091894|FD-Score:-6.11|P-value:3.62E-9 ID:SGTC_3062|Compound:9077494|FD-Score:-5.54|P-value:7.68E-8 ID:SGTC_3142|Compound:9086498|FD-Score:3.42|P-value:5.39E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-3.64|P-value:2.89E-4 ID:SGTC_3210|Compound:9128157|FD-Score:4.32|P-value:1.92E-5 ID:SGTC_3283|Compound:9109332|FD-Score:-6.06|P-value:4.75E-9 ID:SGTC_3298|Compound:9116858|FD-Score:3.62|P-value:2.79E-4 ID:SGTC_3303|Compound:9125618|FD-Score:-3.30|P-value:9.01E-4 ID:SGTC_3304|Compound:9125678|FD-Score:-4.08|P-value:5.69E-5 ID:SGTC_3331|Compound:9141060|FD-Score:3.43|P-value:5.27E-4

Top fitness defect scores for YPR103W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1844 rapamycin 7.38 4.0 nM 1.04E-12 16 7.73E-14 0.08
2 SGTC_137 0438-0332 7.24 85.4 µM 2.74E-12 ubiquinone biosynthesis & proteasome 14 2.25E-13 0.07
3 SGTC_2250 alverine citrate 5.69 130.0 µM 2.89E-8 19 6.19E-9 0.16
4 SGTC_444 curcumin 5.40 90.4 µM 1.35E-7 iron homeostasis 3 3.38E-8 0.09
5 SGTC_879 0831-1030 5.31 162.0 µM 2.13E-7 8 5.59E-8 0.41
6 SGTC_2679 rapamycin 5.24 1.0 nM 2.97E-7 32 8.05E-8 0.02
7 SGTC_2415 st077333 5.21 57.7 µM 3.43E-7 7 9.44E-8 0.07
8 SGTC_64 000l-0018 5.13 272.1 µM 4.99E-7 ubiquinone biosynthesis & proteasome 32 1.43E-7 0.06
9 SGTC_2414 st077232 5.00 92.9 µM 9.48E-7 3 2.89E-7 0.80
10 SGTC_374 0880-0231 5.00 82.3 µM 9.49E-7 iron homeostasis 6 2.89E-7 0.97
11 SGTC_878 0828-0289 4.99 13.7 µM 1.00E-6 15 3.07E-7 0.44
12 SGTC_1083 didemethylchlorpromazine sulfoxide 4.90 707.4 nM 1.51E-6 redox potentiating 18 4.83E-7 0.14
13 SGTC_686 1319-0111 4.82 510.0 µM 2.12E-6 60S ribosome export 4 7.00E-7 0.43
14 SGTC_1597 7-benzyloxygramine 4.79 47.5 µM 2.46E-6 calcium & mitochondrial duress 44 8.24E-7 0.24
15 SGTC_450 aminopyrene 4.68 156.0 nM 4.14E-6 2 1.46E-6 0.11
16 SGTC_2158 5657123 4.62 3.6 µM 5.39E-6 10 1.96E-6 0.03
17 SGTC_201 4262-0159 4.51 12.8 µM 8.57E-6 ergosterol depletion effects on membrane 8 3.26E-6 0.19
18 SGTC_3210 9128157 4.32 49.5 µM 1.92E-5 11 7.90E-6 0.18
19 SGTC_2275 7947463 4.16 200.0 µM 3.64E-5 iron homeostasis 8 1.60E-5 0.02
20 SGTC_2132 5317594 4.14 122.7 µM 3.86E-5 SWF1 & branched chain AA biosynthesis 6 1.71E-5 0.14

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.358 4.32E-102 YFR050C PRE4 het Beta 7 subunit of the 20S proteasome
0.343 6.22E-93 YBL041W PRE7 het Beta 6 subunit of the 20S proteasome
0.301 3.56E-71 YOL038W PRE6 het Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
0.295 1.82E-68 YGL011C SCL1 het Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
0.295 2.52E-68 YMR314W PRE5 het Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.285 8.43E-64 YER012W PRE1 het Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
0.282 2.16E-62 YER094C PUP3 het Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
0.267 1.08E-55 YML092C PRE8 het Alpha 2 subunit of the 20S proteasome
0.265 7.18E-55 YOR362C PRE10 het Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.223 3.71E-39 YGR253C PUP2 het Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
0.219 9.36E-38 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.216 7.45E-37 YDL007W RPT2 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle
0.207 7.46E-34 YJL001W PRE3 het Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides
0.184 7.88E-27 YDR023W SES1 het Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p
0.172 9.88E-24 YLR021W IRC25 hom Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions