YPR108W / RPN7

Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPR108W / RPN7

Click on Significant Values for Screen Details ID:SGTC_115|Compound:0527-0192|FD-Score:-5.00|P-value:8.27E-6 ID:SGTC_126|Compound:0417-1624|FD-Score:4.58|P-value:3.52E-5 ID:SGTC_332|Compound:4112-0506|FD-Score:4.40|P-value:6.80E-5 ID:SGTC_364|Compound:0083-0097|FD-Score:4.61|P-value:3.20E-5 ID:SGTC_513|Compound:4245-1575|FD-Score:-4.79|P-value:1.84E-5 ID:SGTC_593|Compound:1203-0218|FD-Score:-3.75|P-value:6.35E-4 ID:SGTC_608|Compound:k048-0125|FD-Score:-5.24|P-value:3.15E-6 ID:SGTC_1048|Compound:3161-0300|FD-Score:-4.33|P-value:9.55E-5 ID:SGTC_1061|Compound:3448-9325|FD-Score:-5.21|P-value:3.59E-6 ID:SGTC_1082|Compound:camptothecin|FD-Score:3.66|P-value:7.31E-4 ID:SGTC_1499|Compound:4480-0877|FD-Score:-3.72|P-value:6.81E-4 ID:SGTC_1786|Compound:berberine|FD-Score:4.02|P-value:2.40E-4 ID:SGTC_2006|Compound:4011487|FD-Score:3.75|P-value:5.65E-4 ID:SGTC_2016|Compound:5121758|FD-Score:4.91|P-value:1.02E-5 ID:SGTC_2124|Compound:5246320|FD-Score:3.75|P-value:5.60E-4 ID:SGTC_2293|Compound:7624732|FD-Score:3.82|P-value:4.56E-4 ID:SGTC_2335|Compound:9018011|FD-Score:-3.89|P-value:4.15E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:3.92|P-value:3.35E-4 ID:SGTC_2417|Compound:cerulenin|FD-Score:3.96|P-value:2.90E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:-3.75|P-value:6.22E-4 ID:SGTC_2587|Compound:piperic acid|FD-Score:5.45|P-value:1.14E-6 ID:SGTC_2795|Compound:7780010|FD-Score:4.38|P-value:7.18E-5 ID:SGTC_2830|Compound:7992007|FD-Score:4.74|P-value:1.94E-5 ID:SGTC_2833|Compound:9001315|FD-Score:4.09|P-value:1.95E-4 ID:SGTC_2959|Compound:9079155|FD-Score:-4.64|P-value:3.28E-5 ID:SGTC_3219|Compound:9131112|FD-Score:-5.32|P-value:2.28E-6 ID:SGTC_115|Compound:0527-0192|FD-Score:-5.00|P-value:8.27E-6 ID:SGTC_126|Compound:0417-1624|FD-Score:4.58|P-value:3.52E-5 ID:SGTC_332|Compound:4112-0506|FD-Score:4.40|P-value:6.80E-5 ID:SGTC_364|Compound:0083-0097|FD-Score:4.61|P-value:3.20E-5 ID:SGTC_513|Compound:4245-1575|FD-Score:-4.79|P-value:1.84E-5 ID:SGTC_593|Compound:1203-0218|FD-Score:-3.75|P-value:6.35E-4 ID:SGTC_608|Compound:k048-0125|FD-Score:-5.24|P-value:3.15E-6 ID:SGTC_1048|Compound:3161-0300|FD-Score:-4.33|P-value:9.55E-5 ID:SGTC_1061|Compound:3448-9325|FD-Score:-5.21|P-value:3.59E-6 ID:SGTC_1082|Compound:camptothecin|FD-Score:3.66|P-value:7.31E-4 ID:SGTC_1499|Compound:4480-0877|FD-Score:-3.72|P-value:6.81E-4 ID:SGTC_1786|Compound:berberine|FD-Score:4.02|P-value:2.40E-4 ID:SGTC_2006|Compound:4011487|FD-Score:3.75|P-value:5.65E-4 ID:SGTC_2016|Compound:5121758|FD-Score:4.91|P-value:1.02E-5 ID:SGTC_2124|Compound:5246320|FD-Score:3.75|P-value:5.60E-4 ID:SGTC_2293|Compound:7624732|FD-Score:3.82|P-value:4.56E-4 ID:SGTC_2335|Compound:9018011|FD-Score:-3.89|P-value:4.15E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:3.92|P-value:3.35E-4 ID:SGTC_2417|Compound:cerulenin|FD-Score:3.96|P-value:2.90E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:-3.75|P-value:6.22E-4 ID:SGTC_2587|Compound:piperic acid|FD-Score:5.45|P-value:1.14E-6 ID:SGTC_2795|Compound:7780010|FD-Score:4.38|P-value:7.18E-5 ID:SGTC_2830|Compound:7992007|FD-Score:4.74|P-value:1.94E-5 ID:SGTC_2833|Compound:9001315|FD-Score:4.09|P-value:1.95E-4 ID:SGTC_2959|Compound:9079155|FD-Score:-4.64|P-value:3.28E-5 ID:SGTC_3219|Compound:9131112|FD-Score:-5.32|P-value:2.28E-6

Top fitness defect scores for YPR108W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2587 piperic acid 5.45 92.2 µM 1.14E-6 1 2.51E-8 0.88
2 SGTC_2016 5121758 4.91 140.0 µM 1.02E-5 redox potentiating 9 4.52E-7 0.53
3 SGTC_2830 7992007 4.74 71.4 µM 1.94E-5 7 1.06E-6 0.14
4 SGTC_364 0083-0097 4.61 67.7 µM 3.20E-5 4 2.04E-6 0.17
5 SGTC_126 0417-1624 4.58 672.5 µM 3.52E-5 DNA intercalators 9 2.32E-6 0.08
6 SGTC_332 4112-0506 4.40 40.9 µM 6.80E-5 18 5.51E-6 0.21
7 SGTC_2795 7780010 4.38 54.5 µM 7.18E-5 5 5.93E-6 0.05
8 SGTC_2833 9001315 4.09 45.5 µM 1.95E-4 amide catabolism 7 2.20E-5 0.17
9 SGTC_1786 berberine 4.02 21.6 µM 2.40E-4 60S ribosome export 18 2.89E-5 0.02
10 SGTC_2417 cerulenin 3.96 830.0 nM 2.90E-4 11 3.71E-5 0.01
11 SGTC_2409 alverine citrate 3.92 64.0 µM 3.35E-4 14 4.47E-5 0.02
12 SGTC_2293 7624732 3.82 200.0 µM 4.56E-4 8 6.70E-5 0.11
13 SGTC_2124 5246320 3.75 200.0 µM 5.60E-4 heme biosynthesis & mitochondrial translocase 32 8.78E-5 0.09
14 SGTC_2006 4011487 3.75 200.0 µM 5.65E-4 endomembrane recycling 20 8.89E-5 0.06
15 SGTC_1082 camptothecin 3.66 424.9 nM 7.31E-4 DNA damage response 22 1.24E-4 0.01
16 SGTC_225 nsc-122657 3.48 1.0 µM 0.00124 6 2.49E-4 0.30
17 SGTC_123 0083-0120 3.48 66.7 µM 0.00125 15 2.51E-4 0.04
18 SGTC_1775 st081588 3.44 35.2 µM 0.00142 60S ribosome export 15 2.96E-4 0.02
19 SGTC_3325 9138760 3.41 55.2 µM 0.00150 Golgi 6 3.19E-4 0.01
20 SGTC_1149 3909-7961 3.35 23.3 µM 0.00181 11 4.08E-4 0.18

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.202 4.17E-32 YOR259C RPT4 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
0.145 2.54E-17 YDR394W RPT3 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
0.113 5.12E-11 YBL028C YBL028C hom Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis
0.085 8.17E-7 YHR020W YHR020W het Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments
0.085 9.11E-7 YGR204W ADE3 hom Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine
0.084 1.07E-6 YFR052W RPN12 het Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress
0.081 2.93E-6 YKR062W TFA2 het TFIIE small subunit, involved in RNA polymerase II transcription initiation
0.080 3.28E-6 YBL030C PET9 het Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication
0.080 3.82E-6 YPR066W UBA3 hom Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
0.075 1.22E-5 YGL152C_d YGL152C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF PEX14/YGL153W
0.075 1.41E-5 YLR327C TMA10 hom Protein of unknown function that associates with ribosomes; putative homolog of the F1F0-ATPase synthase regulator Stf2p; protein abundance increases in response to DNA replication stress
0.075 1.35E-5 YDR232W HEM1 het 5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p
0.073 2.57E-5 YMR300C ADE4 hom Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway
0.072 3.26E-5 YMR306W FKS3 hom Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.070 5.40E-5 YLR260W LCB5 hom Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules