YPR111W / DBF20

Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs

Zygosity: Homozygous strain
fixedexpanded
Profile for YPR111W / DBF20

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:5.06|P-value:8.52E-9 ID:SGTC_19|Compound:0279-0214|FD-Score:2.90|P-value:5.91E-4 ID:SGTC_34|Compound:0469-0683|FD-Score:-4.40|P-value:5.55E-7 ID:SGTC_136|Compound:0097-0015|FD-Score:3.01|P-value:3.81E-4 ID:SGTC_341|Compound:1486-1430|FD-Score:-5.30|P-value:2.11E-9 ID:SGTC_344|Compound:k064-0035|FD-Score:5.12|P-value:5.83E-9 ID:SGTC_543|Compound:3844-1095|FD-Score:-3.11|P-value:2.91E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:3.25|P-value:1.42E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:3.02|P-value:3.68E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:3.82|P-value:1.01E-5 ID:SGTC_878|Compound:0828-0289|FD-Score:-2.81|P-value:9.36E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:-3.67|P-value:2.44E-5 ID:SGTC_942|Compound:3253-1115|FD-Score:4.46|P-value:3.27E-7 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.16|P-value:2.36E-4 ID:SGTC_1233|Compound:0325-0408|FD-Score:2.99|P-value:4.23E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.09|P-value:2.76E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:4.03|P-value:3.44E-6 ID:SGTC_1571|Compound:st070080|FD-Score:-3.15|P-value:2.45E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:-2.88|P-value:7.09E-4 ID:SGTC_1676|Compound:st018489|FD-Score:3.54|P-value:3.94E-5 ID:SGTC_1748|Compound:st037455|FD-Score:4.15|P-value:1.85E-6 ID:SGTC_1773|Compound:st048542|FD-Score:3.24|P-value:1.49E-4 ID:SGTC_1838|Compound:st055369|FD-Score:3.66|P-value:2.15E-5 ID:SGTC_1862|Compound:st057513|FD-Score:3.47|P-value:5.29E-5 ID:SGTC_2260|Compound:7964312|FD-Score:-3.48|P-value:5.95E-5 ID:SGTC_2274|Compound:7947094|FD-Score:3.71|P-value:1.74E-5 ID:SGTC_2286|Compound:7945697|FD-Score:2.80|P-value:8.66E-4 ID:SGTC_2289|Compound:7946404|FD-Score:3.05|P-value:3.28E-4 ID:SGTC_2482|Compound:5981709|FD-Score:-3.15|P-value:2.49E-4 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:-2.84|P-value:8.50E-4 ID:SGTC_16|Compound:erodoxin|FD-Score:5.06|P-value:8.52E-9 ID:SGTC_19|Compound:0279-0214|FD-Score:2.90|P-value:5.91E-4 ID:SGTC_34|Compound:0469-0683|FD-Score:-4.40|P-value:5.55E-7 ID:SGTC_136|Compound:0097-0015|FD-Score:3.01|P-value:3.81E-4 ID:SGTC_341|Compound:1486-1430|FD-Score:-5.30|P-value:2.11E-9 ID:SGTC_344|Compound:k064-0035|FD-Score:5.12|P-value:5.83E-9 ID:SGTC_543|Compound:3844-1095|FD-Score:-3.11|P-value:2.91E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:3.25|P-value:1.42E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:3.02|P-value:3.68E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:3.82|P-value:1.01E-5 ID:SGTC_878|Compound:0828-0289|FD-Score:-2.81|P-value:9.36E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:-3.67|P-value:2.44E-5 ID:SGTC_942|Compound:3253-1115|FD-Score:4.46|P-value:3.27E-7 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.16|P-value:2.36E-4 ID:SGTC_1233|Compound:0325-0408|FD-Score:2.99|P-value:4.23E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.09|P-value:2.76E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:4.03|P-value:3.44E-6 ID:SGTC_1571|Compound:st070080|FD-Score:-3.15|P-value:2.45E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:-2.88|P-value:7.09E-4 ID:SGTC_1676|Compound:st018489|FD-Score:3.54|P-value:3.94E-5 ID:SGTC_1748|Compound:st037455|FD-Score:4.15|P-value:1.85E-6 ID:SGTC_1773|Compound:st048542|FD-Score:3.24|P-value:1.49E-4 ID:SGTC_1838|Compound:st055369|FD-Score:3.66|P-value:2.15E-5 ID:SGTC_1862|Compound:st057513|FD-Score:3.47|P-value:5.29E-5 ID:SGTC_2260|Compound:7964312|FD-Score:-3.48|P-value:5.95E-5 ID:SGTC_2274|Compound:7947094|FD-Score:3.71|P-value:1.74E-5 ID:SGTC_2286|Compound:7945697|FD-Score:2.80|P-value:8.66E-4 ID:SGTC_2289|Compound:7946404|FD-Score:3.05|P-value:3.28E-4 ID:SGTC_2482|Compound:5981709|FD-Score:-3.15|P-value:2.49E-4 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:-2.84|P-value:8.50E-4

Top fitness defect scores for YPR111W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_344 k064-0035 5.12 6.4 µM 5.83E-9 60S ribosome export 10 1.56E-7
2 SGTC_16 erodoxin 5.06 22.8 µM 8.52E-9 unfolded protein response 38 2.12E-7
3 SGTC_942 3253-1115 4.46 227.0 µM 3.27E-7 18 4.12E-6
4 SGTC_1748 st037455 4.15 53.6 µM 1.85E-6 24 1.68E-5
5 SGTC_1511 0906-3109 4.03 28.5 µM 3.44E-6 excess fatty acid 44 2.79E-5
6 SGTC_872 0588-0139 3.82 234.0 µM 1.01E-5 38 6.68E-5
7 SGTC_2274 7947094 3.71 46.1 µM 1.74E-5 43 1.04E-4
8 SGTC_1838 st055369 3.66 84.5 µM 2.15E-5 26 1.24E-4
9 SGTC_1676 st018489 3.54 23.4 µM 3.94E-5 60S ribosome export 50 2.03E-4
10 SGTC_1862 st057513 3.47 3.8 µM 5.29E-5 27 2.58E-4
11 SGTC_770 0389-1121 3.25 67.2 µM 1.42E-4 RPP1 & pyrimidine depletion 58 5.78E-4
12 SGTC_1773 st048542 3.24 73.6 µM 1.49E-4 79 6.00E-4
13 SGTC_1378 2093-0007 3.09 380.0 nM 2.76E-4 58 9.94E-4
14 SGTC_2289 7946404 3.05 165.1 µM 3.28E-4 152 0.00114
15 SGTC_836 0330-0104 3.02 1.4 µM 3.68E-4 49 0.00126
16 SGTC_136 0097-0015 3.01 404.5 µM 3.81E-4 60S ribosome export 63 0.00129
17 SGTC_1233 0325-0408 2.99 14.2 µM 4.23E-4 59 0.00141
18 SGTC_19 0279-0214 2.90 38.4 µM 5.91E-4 TRP & mitochondrial translation 125 0.00185
19 SGTC_2286 7945697 2.80 125.0 µM 8.66E-4 124 0.00253
20 SGTC_1384 3089-0840 2.76 101.0 µM 0.00101 69 0.00288

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.187 1.02E-27 YPL244C HUT1 hom Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1
0.182 1.87E-26 YDL045C FAD1 het Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin
0.161 4.92E-21 YDR169C-A_p YDR169C-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.158 3.17E-20 YML130C ERO1 het Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
0.137 2.14E-15 YLR307W CDA1 hom Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall
0.133 9.94E-15 YPL243W SRP68 het Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
0.126 2.29E-13 YMR161W HLJ1 hom Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ
0.115 2.61E-11 YFL031W HAC1 hom Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress
0.111 1.20E-10 YHL020C OPI1 hom Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance
0.102 3.12E-9 YPL123C RNY1 hom Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity
0.097 1.80E-8 YJR119C JHD2 hom JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation
0.094 4.34E-8 YNR008W LRO1 hom Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase
0.093 5.98E-8 YER087C-B SBH1 hom Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p
0.092 8.12E-8 YAL037W_p YAL037W_p hom Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication
0.091 1.48E-7 YNL322C KRE1 hom Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor