YPR112C / MRD1

Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPR112C / MRD1

Click on Significant Values for Screen Details ID:SGTC_307|Compound:1501-1223|FD-Score:3.83|P-value:8.58E-5 ID:SGTC_359|Compound:0081-0058|FD-Score:3.42|P-value:3.98E-4 ID:SGTC_401|Compound:neomycin|FD-Score:-4.32|P-value:1.08E-5 ID:SGTC_412|Compound:gemfibrozil|FD-Score:3.26|P-value:7.00E-4 ID:SGTC_440|Compound:fluspirilene|FD-Score:-4.45|P-value:5.95E-6 ID:SGTC_448|Compound:monastrol|FD-Score:3.41|P-value:4.14E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:3.20|P-value:8.45E-4 ID:SGTC_646|Compound:4092-0839|FD-Score:3.54|P-value:2.60E-4 ID:SGTC_680|Compound:0417-1641|FD-Score:3.66|P-value:1.68E-4 ID:SGTC_965|Compound:1291-5240|FD-Score:6.52|P-value:8.13E-11 ID:SGTC_1011|Compound:4112-3381|FD-Score:-4.12|P-value:2.51E-5 ID:SGTC_1015|Compound:4130-4525|FD-Score:4.56|P-value:3.83E-6 ID:SGTC_1066|Compound:ketoconazole|FD-Score:3.59|P-value:2.14E-4 ID:SGTC_1069|Compound:diphenylcyclopropenone|FD-Score:4.12|P-value:2.66E-5 ID:SGTC_1070|Compound:indatraline|FD-Score:3.26|P-value:6.95E-4 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:4.28|P-value:1.34E-5 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:3.91|P-value:6.38E-5 ID:SGTC_1128|Compound:3448-9350|FD-Score:-3.42|P-value:3.83E-4 ID:SGTC_1196|Compound:1588-0294|FD-Score:4.89|P-value:8.32E-7 ID:SGTC_1202|Compound:1988-0068|FD-Score:5.12|P-value:2.54E-7 ID:SGTC_1272|Compound:0839-0021|FD-Score:3.47|P-value:3.30E-4 ID:SGTC_1299|Compound:1079-1439|FD-Score:-3.31|P-value:5.64E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:4.87|P-value:8.75E-7 ID:SGTC_1392|Compound:3455-1037|FD-Score:3.36|P-value:4.98E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.26|P-value:6.89E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:-3.32|P-value:5.46E-4 ID:SGTC_1619|Compound:st003326|FD-Score:3.79|P-value:1.02E-4 ID:SGTC_1732|Compound:st034308|FD-Score:-3.74|P-value:1.17E-4 ID:SGTC_1735|Compound:st034310|FD-Score:-3.15|P-value:9.56E-4 ID:SGTC_1818|Compound:st052136|FD-Score:3.87|P-value:7.47E-5 ID:SGTC_1830|Compound:st055982|FD-Score:4.60|P-value:3.21E-6 ID:SGTC_1834|Compound:st055981|FD-Score:3.18|P-value:9.14E-4 ID:SGTC_1837|Compound:st055472|FD-Score:6.61|P-value:4.60E-11 ID:SGTC_1843|Compound:st055809|FD-Score:3.86|P-value:7.70E-5 ID:SGTC_1958|Compound:st076513|FD-Score:4.00|P-value:4.41E-5 ID:SGTC_2236|Compound:dapsone|FD-Score:-3.25|P-value:6.82E-4 ID:SGTC_2244|Compound:6635614|FD-Score:4.21|P-value:1.81E-5 ID:SGTC_2328|Compound:6201408|FD-Score:3.54|P-value:2.64E-4 ID:SGTC_2403|Compound:6032726|FD-Score:-3.31|P-value:5.69E-4 ID:SGTC_2432|Compound:5630864|FD-Score:-4.00|P-value:4.11E-5 ID:SGTC_2482|Compound:5981709|FD-Score:4.44|P-value:6.63E-6 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:-3.46|P-value:3.36E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.27|P-value:6.70E-4 ID:SGTC_2608|Compound:thymoquinone|FD-Score:4.94|P-value:6.31E-7 ID:SGTC_2612|Compound:dictamnine|FD-Score:4.04|P-value:3.72E-5 ID:SGTC_2616|Compound:abietic acid|FD-Score:3.66|P-value:1.66E-4 ID:SGTC_2698|Compound:st077697|FD-Score:4.70|P-value:1.99E-6 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.55|P-value:2.55E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:3.47|P-value:3.37E-4 ID:SGTC_2810|Compound:7959871|FD-Score:10.20|P-value:8.70E-24 ID:SGTC_2815|Compound:7968870|FD-Score:-3.21|P-value:8.06E-4 ID:SGTC_2827|Compound:7993847|FD-Score:3.21|P-value:8.19E-4 ID:SGTC_2915|Compound:7971052|FD-Score:3.54|P-value:2.61E-4 ID:SGTC_2917|Compound:7979103|FD-Score:-3.22|P-value:7.65E-4 ID:SGTC_2964|Compound:9083345|FD-Score:4.11|P-value:2.83E-5 ID:SGTC_3014|Compound:9081533|FD-Score:4.01|P-value:4.16E-5 ID:SGTC_3052|Compound:9092717|FD-Score:-3.23|P-value:7.41E-4 ID:SGTC_3056|Compound:9094900|FD-Score:-4.16|P-value:2.14E-5 ID:SGTC_3131|Compound:9127911|FD-Score:4.49|P-value:5.46E-6 ID:SGTC_3175|Compound:9106517|FD-Score:3.33|P-value:5.60E-4 ID:SGTC_3176|Compound:9106582|FD-Score:4.09|P-value:3.08E-5 ID:SGTC_3179|Compound:9107801|FD-Score:-6.14|P-value:8.16E-10 ID:SGTC_3208|Compound:9114115|FD-Score:-3.20|P-value:8.10E-4 ID:SGTC_307|Compound:1501-1223|FD-Score:3.83|P-value:8.58E-5 ID:SGTC_359|Compound:0081-0058|FD-Score:3.42|P-value:3.98E-4 ID:SGTC_401|Compound:neomycin|FD-Score:-4.32|P-value:1.08E-5 ID:SGTC_412|Compound:gemfibrozil|FD-Score:3.26|P-value:7.00E-4 ID:SGTC_440|Compound:fluspirilene|FD-Score:-4.45|P-value:5.95E-6 ID:SGTC_448|Compound:monastrol|FD-Score:3.41|P-value:4.14E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:3.20|P-value:8.45E-4 ID:SGTC_646|Compound:4092-0839|FD-Score:3.54|P-value:2.60E-4 ID:SGTC_680|Compound:0417-1641|FD-Score:3.66|P-value:1.68E-4 ID:SGTC_965|Compound:1291-5240|FD-Score:6.52|P-value:8.13E-11 ID:SGTC_1011|Compound:4112-3381|FD-Score:-4.12|P-value:2.51E-5 ID:SGTC_1015|Compound:4130-4525|FD-Score:4.56|P-value:3.83E-6 ID:SGTC_1066|Compound:ketoconazole|FD-Score:3.59|P-value:2.14E-4 ID:SGTC_1069|Compound:diphenylcyclopropenone|FD-Score:4.12|P-value:2.66E-5 ID:SGTC_1070|Compound:indatraline|FD-Score:3.26|P-value:6.95E-4 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:4.28|P-value:1.34E-5 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:3.91|P-value:6.38E-5 ID:SGTC_1128|Compound:3448-9350|FD-Score:-3.42|P-value:3.83E-4 ID:SGTC_1196|Compound:1588-0294|FD-Score:4.89|P-value:8.32E-7 ID:SGTC_1202|Compound:1988-0068|FD-Score:5.12|P-value:2.54E-7 ID:SGTC_1272|Compound:0839-0021|FD-Score:3.47|P-value:3.30E-4 ID:SGTC_1299|Compound:1079-1439|FD-Score:-3.31|P-value:5.64E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:4.87|P-value:8.75E-7 ID:SGTC_1392|Compound:3455-1037|FD-Score:3.36|P-value:4.98E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.26|P-value:6.89E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:-3.32|P-value:5.46E-4 ID:SGTC_1619|Compound:st003326|FD-Score:3.79|P-value:1.02E-4 ID:SGTC_1732|Compound:st034308|FD-Score:-3.74|P-value:1.17E-4 ID:SGTC_1735|Compound:st034310|FD-Score:-3.15|P-value:9.56E-4 ID:SGTC_1818|Compound:st052136|FD-Score:3.87|P-value:7.47E-5 ID:SGTC_1830|Compound:st055982|FD-Score:4.60|P-value:3.21E-6 ID:SGTC_1834|Compound:st055981|FD-Score:3.18|P-value:9.14E-4 ID:SGTC_1837|Compound:st055472|FD-Score:6.61|P-value:4.60E-11 ID:SGTC_1843|Compound:st055809|FD-Score:3.86|P-value:7.70E-5 ID:SGTC_1958|Compound:st076513|FD-Score:4.00|P-value:4.41E-5 ID:SGTC_2236|Compound:dapsone|FD-Score:-3.25|P-value:6.82E-4 ID:SGTC_2244|Compound:6635614|FD-Score:4.21|P-value:1.81E-5 ID:SGTC_2328|Compound:6201408|FD-Score:3.54|P-value:2.64E-4 ID:SGTC_2403|Compound:6032726|FD-Score:-3.31|P-value:5.69E-4 ID:SGTC_2432|Compound:5630864|FD-Score:-4.00|P-value:4.11E-5 ID:SGTC_2482|Compound:5981709|FD-Score:4.44|P-value:6.63E-6 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:-3.46|P-value:3.36E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.27|P-value:6.70E-4 ID:SGTC_2608|Compound:thymoquinone|FD-Score:4.94|P-value:6.31E-7 ID:SGTC_2612|Compound:dictamnine|FD-Score:4.04|P-value:3.72E-5 ID:SGTC_2616|Compound:abietic acid|FD-Score:3.66|P-value:1.66E-4 ID:SGTC_2698|Compound:st077697|FD-Score:4.70|P-value:1.99E-6 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.55|P-value:2.55E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:3.47|P-value:3.37E-4 ID:SGTC_2810|Compound:7959871|FD-Score:10.20|P-value:8.70E-24 ID:SGTC_2815|Compound:7968870|FD-Score:-3.21|P-value:8.06E-4 ID:SGTC_2827|Compound:7993847|FD-Score:3.21|P-value:8.19E-4 ID:SGTC_2915|Compound:7971052|FD-Score:3.54|P-value:2.61E-4 ID:SGTC_2917|Compound:7979103|FD-Score:-3.22|P-value:7.65E-4 ID:SGTC_2964|Compound:9083345|FD-Score:4.11|P-value:2.83E-5 ID:SGTC_3014|Compound:9081533|FD-Score:4.01|P-value:4.16E-5 ID:SGTC_3052|Compound:9092717|FD-Score:-3.23|P-value:7.41E-4 ID:SGTC_3056|Compound:9094900|FD-Score:-4.16|P-value:2.14E-5 ID:SGTC_3131|Compound:9127911|FD-Score:4.49|P-value:5.46E-6 ID:SGTC_3175|Compound:9106517|FD-Score:3.33|P-value:5.60E-4 ID:SGTC_3176|Compound:9106582|FD-Score:4.09|P-value:3.08E-5 ID:SGTC_3179|Compound:9107801|FD-Score:-6.14|P-value:8.16E-10 ID:SGTC_3208|Compound:9114115|FD-Score:-3.20|P-value:8.10E-4

Top fitness defect scores for YPR112C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2810 7959871 10.20 45.5 µM 8.70E-24 copper-dependent oxidative stress 1 1.25E-24 3.61
2 SGTC_1837 st055472 6.61 66.5 µM 4.60E-11 1 1.98E-11 1.88
3 SGTC_965 1291-5240 6.52 308.0 µM 8.13E-11 1 3.58E-11 2.34
4 SGTC_1202 1988-0068 5.12 29.6 µM 2.54E-7 RNA processing & uracil transport 5 1.51E-7 0.40
5 SGTC_2608 thymoquinone 4.94 42.7 µM 6.31E-7 2 3.88E-7 1.27
6 SGTC_1196 1588-0294 4.89 184.0 µM 8.32E-7 2 5.16E-7 0.18
7 SGTC_1368 1852-0141 4.87 284.0 µM 8.75E-7 ERG2 1 5.44E-7 0.83
8 SGTC_2698 st077697 4.70 67.5 µM 1.99E-6 3 1.28E-6 0.01
9 SGTC_1830 st055982 4.60 50.3 µM 3.21E-6 2 2.10E-6 0.74
10 SGTC_1015 4130-4525 4.56 82.0 µM 3.83E-6 60S ribosome export 7 2.51E-6 0.57
11 SGTC_3131 9127911 4.49 49.5 µM 5.46E-6 6 3.63E-6 0.31
12 SGTC_2482 5981709 4.44 142.7 µM 6.63E-6 PDR1 10 4.44E-6 0.17
13 SGTC_1071 trifluoperazine 4.28 6.3 µM 1.34E-5 5 9.21E-6 0.01
14 SGTC_2244 6635614 4.21 200.0 µM 1.81E-5 2 1.26E-5 0.77
15 SGTC_1069 diphenylcyclopropenone 4.12 8.5 µM 2.66E-5 7 1.87E-5 0.05
16 SGTC_2964 9083345 4.11 53.4 µM 2.83E-5 12 2.00E-5 0.12
17 SGTC_3176 9106582 4.09 49.5 µM 3.08E-5 1 2.18E-5 0.65
18 SGTC_2612 dictamnine 4.04 15.5 µM 3.72E-5 RNA pol III & RNase P/MRP 29 2.66E-5 0.03
19 SGTC_3014 9081533 4.01 49.5 µM 4.16E-5 3 2.99E-5 0.24
20 SGTC_1958 st076513 4.00 41.9 µM 4.41E-5 60S ribosome export 5 3.17E-5 0.50

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.127 1.64E-13 YOR005C DNL4 hom DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth
0.094 5.79E-8 YDR498C SEC20 het Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p
0.085 8.43E-7 YOL043C NTG2 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication
0.078 5.51E-6 YHR085W IPI1 het Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; IPI1 is an essential gene
0.078 5.88E-6 YGL099W LSG1 het Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
0.077 8.46E-6 YDR098C GRX3 hom Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36
0.073 2.44E-5 YBR141C_p YBR141C_p hom Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene
0.070 4.36E-5 YDL076C RXT3 hom Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress
0.070 4.89E-5 YDR384C ATO3 hom Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters
0.070 4.94E-5 YML050W AIM32 hom Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss
0.070 5.10E-5 YFL004W VTC2 hom Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion
0.068 8.70E-5 YBR276C PPS1 hom Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle
0.067 9.26E-5 YOR294W RRS1 het Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
0.066 1.24E-4 YDR353W TRR1 het Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication
0.066 1.31E-4 YNL142W MEP2 hom Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation