YPR131C / NAT3

Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met

Zygosity: Homozygous strain
fixedexpanded
Profile for YPR131C / NAT3

Click on Significant Values for Screen Details ID:SGTC_68|Compound:0986-0284|FD-Score:3.39|P-value:3.09E-4 ID:SGTC_101|Compound:0490-5701|FD-Score:3.54|P-value:1.79E-4 ID:SGTC_175|Compound:3793-3120|FD-Score:-3.52|P-value:1.90E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:3.52|P-value:1.90E-4 ID:SGTC_308|Compound:4092-1169|FD-Score:4.80|P-value:6.53E-7 ID:SGTC_495|Compound:decylubiquinone|FD-Score:-3.07|P-value:9.53E-4 ID:SGTC_536|Compound:3852-0578|FD-Score:-3.66|P-value:1.10E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:3.19|P-value:6.44E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:4.33|P-value:6.24E-6 ID:SGTC_673|Compound:1082-0474|FD-Score:3.30|P-value:4.31E-4 ID:SGTC_675|Compound:1309-0189|FD-Score:3.76|P-value:7.60E-5 ID:SGTC_707|Compound:0865-0008|FD-Score:3.42|P-value:2.84E-4 ID:SGTC_740|Compound:k014-0057|FD-Score:3.67|P-value:1.06E-4 ID:SGTC_791|Compound:1348-1292|FD-Score:3.62|P-value:1.33E-4 ID:SGTC_883|Compound:0850-0245|FD-Score:-3.14|P-value:7.50E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:4.04|P-value:2.32E-5 ID:SGTC_963|Compound:1269-2853|FD-Score:3.41|P-value:2.94E-4 ID:SGTC_981|Compound:1488-1094|FD-Score:3.54|P-value:1.81E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:7.29|P-value:9.70E-14 ID:SGTC_1245|Compound:0418-0076|FD-Score:-3.37|P-value:3.32E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:-4.79|P-value:6.52E-7 ID:SGTC_1394|Compound:3474-0001|FD-Score:4.40|P-value:4.49E-6 ID:SGTC_1526|Compound:st051518|FD-Score:-3.20|P-value:6.07E-4 ID:SGTC_1622|Compound:st003707|FD-Score:3.69|P-value:9.80E-5 ID:SGTC_1712|Compound:st032288|FD-Score:4.19|P-value:1.16E-5 ID:SGTC_2015|Compound:5119600|FD-Score:3.83|P-value:5.67E-5 ID:SGTC_2040|Compound:5100729|FD-Score:5.47|P-value:1.76E-8 ID:SGTC_2267|Compound:7931562|FD-Score:3.40|P-value:3.02E-4 ID:SGTC_2268|Compound:7933382|FD-Score:3.51|P-value:2.00E-4 ID:SGTC_2333|Compound:9017922|FD-Score:4.34|P-value:6.02E-6 ID:SGTC_2354|Compound:9060492|FD-Score:3.91|P-value:3.93E-5 ID:SGTC_2358|Compound:9037157|FD-Score:4.12|P-value:1.62E-5 ID:SGTC_2445|Compound:5742597|FD-Score:3.67|P-value:1.06E-4 ID:SGTC_2457|Compound:5731572|FD-Score:4.35|P-value:5.70E-6 ID:SGTC_2458|Compound:5808907|FD-Score:4.00|P-value:2.79E-5 ID:SGTC_2471|Compound:5466956|FD-Score:3.49|P-value:2.14E-4 ID:SGTC_2475|Compound:5812728|FD-Score:4.44|P-value:3.80E-6 ID:SGTC_2486|Compound:5283202|FD-Score:5.10|P-value:1.34E-7 ID:SGTC_2556|Compound:citropten|FD-Score:3.20|P-value:6.29E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:3.44|P-value:2.65E-4 ID:SGTC_2682|Compound:pyrogallin|FD-Score:4.86|P-value:4.71E-7 ID:SGTC_2939|Compound:9044578|FD-Score:-3.43|P-value:2.68E-4 ID:SGTC_2988|Compound:9073640|FD-Score:7.19|P-value:2.07E-13 ID:SGTC_3033|Compound:9090887|FD-Score:3.17|P-value:6.96E-4 ID:SGTC_3049|Compound:9092223|FD-Score:-3.33|P-value:3.86E-4 ID:SGTC_3069|Compound:9117009|FD-Score:3.49|P-value:2.18E-4 ID:SGTC_3071|Compound:9117417|FD-Score:-3.30|P-value:4.31E-4 ID:SGTC_3077|Compound:9117319|FD-Score:-3.79|P-value:6.59E-5 ID:SGTC_3078|Compound:9116413|FD-Score:4.07|P-value:1.99E-5 ID:SGTC_3087|Compound:9117406|FD-Score:3.69|P-value:1.01E-4 ID:SGTC_3131|Compound:9127911|FD-Score:-3.66|P-value:1.09E-4 ID:SGTC_3204|Compound:9114183|FD-Score:-3.93|P-value:3.66E-5 ID:SGTC_3205|Compound:9113483|FD-Score:-3.21|P-value:6.03E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-3.38|P-value:3.26E-4 ID:SGTC_3273|Compound:9137859|FD-Score:3.90|P-value:4.25E-5 ID:SGTC_3303|Compound:9125618|FD-Score:-3.21|P-value:6.03E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:3.39|P-value:3.09E-4 ID:SGTC_101|Compound:0490-5701|FD-Score:3.54|P-value:1.79E-4 ID:SGTC_175|Compound:3793-3120|FD-Score:-3.52|P-value:1.90E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:3.52|P-value:1.90E-4 ID:SGTC_308|Compound:4092-1169|FD-Score:4.80|P-value:6.53E-7 ID:SGTC_495|Compound:decylubiquinone|FD-Score:-3.07|P-value:9.53E-4 ID:SGTC_536|Compound:3852-0578|FD-Score:-3.66|P-value:1.10E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:3.19|P-value:6.44E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:4.33|P-value:6.24E-6 ID:SGTC_673|Compound:1082-0474|FD-Score:3.30|P-value:4.31E-4 ID:SGTC_675|Compound:1309-0189|FD-Score:3.76|P-value:7.60E-5 ID:SGTC_707|Compound:0865-0008|FD-Score:3.42|P-value:2.84E-4 ID:SGTC_740|Compound:k014-0057|FD-Score:3.67|P-value:1.06E-4 ID:SGTC_791|Compound:1348-1292|FD-Score:3.62|P-value:1.33E-4 ID:SGTC_883|Compound:0850-0245|FD-Score:-3.14|P-value:7.50E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:4.04|P-value:2.32E-5 ID:SGTC_963|Compound:1269-2853|FD-Score:3.41|P-value:2.94E-4 ID:SGTC_981|Compound:1488-1094|FD-Score:3.54|P-value:1.81E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:7.29|P-value:9.70E-14 ID:SGTC_1245|Compound:0418-0076|FD-Score:-3.37|P-value:3.32E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:-4.79|P-value:6.52E-7 ID:SGTC_1394|Compound:3474-0001|FD-Score:4.40|P-value:4.49E-6 ID:SGTC_1526|Compound:st051518|FD-Score:-3.20|P-value:6.07E-4 ID:SGTC_1622|Compound:st003707|FD-Score:3.69|P-value:9.80E-5 ID:SGTC_1712|Compound:st032288|FD-Score:4.19|P-value:1.16E-5 ID:SGTC_2015|Compound:5119600|FD-Score:3.83|P-value:5.67E-5 ID:SGTC_2040|Compound:5100729|FD-Score:5.47|P-value:1.76E-8 ID:SGTC_2267|Compound:7931562|FD-Score:3.40|P-value:3.02E-4 ID:SGTC_2268|Compound:7933382|FD-Score:3.51|P-value:2.00E-4 ID:SGTC_2333|Compound:9017922|FD-Score:4.34|P-value:6.02E-6 ID:SGTC_2354|Compound:9060492|FD-Score:3.91|P-value:3.93E-5 ID:SGTC_2358|Compound:9037157|FD-Score:4.12|P-value:1.62E-5 ID:SGTC_2445|Compound:5742597|FD-Score:3.67|P-value:1.06E-4 ID:SGTC_2457|Compound:5731572|FD-Score:4.35|P-value:5.70E-6 ID:SGTC_2458|Compound:5808907|FD-Score:4.00|P-value:2.79E-5 ID:SGTC_2471|Compound:5466956|FD-Score:3.49|P-value:2.14E-4 ID:SGTC_2475|Compound:5812728|FD-Score:4.44|P-value:3.80E-6 ID:SGTC_2486|Compound:5283202|FD-Score:5.10|P-value:1.34E-7 ID:SGTC_2556|Compound:citropten|FD-Score:3.20|P-value:6.29E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:3.44|P-value:2.65E-4 ID:SGTC_2682|Compound:pyrogallin|FD-Score:4.86|P-value:4.71E-7 ID:SGTC_2939|Compound:9044578|FD-Score:-3.43|P-value:2.68E-4 ID:SGTC_2988|Compound:9073640|FD-Score:7.19|P-value:2.07E-13 ID:SGTC_3033|Compound:9090887|FD-Score:3.17|P-value:6.96E-4 ID:SGTC_3049|Compound:9092223|FD-Score:-3.33|P-value:3.86E-4 ID:SGTC_3069|Compound:9117009|FD-Score:3.49|P-value:2.18E-4 ID:SGTC_3071|Compound:9117417|FD-Score:-3.30|P-value:4.31E-4 ID:SGTC_3077|Compound:9117319|FD-Score:-3.79|P-value:6.59E-5 ID:SGTC_3078|Compound:9116413|FD-Score:4.07|P-value:1.99E-5 ID:SGTC_3087|Compound:9117406|FD-Score:3.69|P-value:1.01E-4 ID:SGTC_3131|Compound:9127911|FD-Score:-3.66|P-value:1.09E-4 ID:SGTC_3204|Compound:9114183|FD-Score:-3.93|P-value:3.66E-5 ID:SGTC_3205|Compound:9113483|FD-Score:-3.21|P-value:6.03E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-3.38|P-value:3.26E-4 ID:SGTC_3273|Compound:9137859|FD-Score:3.90|P-value:4.25E-5 ID:SGTC_3303|Compound:9125618|FD-Score:-3.21|P-value:6.03E-4

Top fitness defect scores for YPR131C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1136 pergolide 7.29 1.1 µM 9.70E-14 4 1.57E-13
2 SGTC_2988 9073640 7.19 71.4 µM 2.07E-13 2 3.32E-13
3 SGTC_2040 5100729 5.47 123.0 µM 1.76E-8 15 2.31E-8
4 SGTC_2486 5283202 5.10 22.4 µM 1.34E-7 18 1.71E-7
5 SGTC_2682 pyrogallin 4.86 40.0 µM 4.71E-7 copper-dependent oxidative stress 14 5.85E-7
6 SGTC_308 4092-1169 4.80 104.0 µM 6.53E-7 plasma membrane duress 33 8.06E-7
7 SGTC_2475 5812728 4.44 15.0 µM 3.80E-6 mitochondrial processes 14 4.55E-6
8 SGTC_1394 3474-0001 4.40 65.7 µM 4.49E-6 endomembrane recycling 29 5.37E-6
9 SGTC_2457 5731572 4.35 11.7 µM 5.70E-6 12 6.79E-6
10 SGTC_2333 9017922 4.34 200.0 µM 6.02E-6 18 7.16E-6
11 SGTC_625 k213-0058 4.33 121.0 µM 6.24E-6 translation 81 7.42E-6
12 SGTC_1712 st032288 4.19 53.4 µM 1.16E-5 calcium & mitochondrial duress 170 1.37E-5
13 SGTC_2358 9037157 4.12 4.0 µM 1.62E-5 27 1.89E-5
14 SGTC_3078 9116413 4.07 49.5 µM 1.99E-5 unfolded protein response 34 2.32E-5
15 SGTC_951 1171-0579 4.04 82.7 µM 2.32E-5 cell wall 79 2.70E-5
16 SGTC_2458 5808907 4.00 16.4 µM 2.79E-5 5 3.23E-5
17 SGTC_2354 9060492 3.91 162.0 µM 3.93E-5 18 4.53E-5
18 SGTC_3273 9137859 3.90 49.5 µM 4.25E-5 4 4.89E-5
19 SGTC_2015 5119600 3.83 184.0 µM 5.67E-5 34 6.49E-5
20 SGTC_675 1309-0189 3.76 238.0 µM 7.60E-5 16 8.66E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.137 1.47E-15 YLL013C PUF3 hom Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins
0.109 2.69E-10 YMR113W FOL3 het Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis
0.107 4.81E-10 YPL266W DIM1 het Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing
0.103 2.04E-9 YLR416C_d YLR416C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.094 5.67E-8 YGR214W RPS0A hom Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication;
0.090 1.97E-7 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.089 2.74E-7 YBR245C ISW1 hom ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions
0.088 3.11E-7 YER120W SCS2 hom Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication
0.088 3.48E-7 YER016W BIM1 hom Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally
0.086 5.49E-7 YOL038C-A_p YOL038C-A_p hom Putative protein of unknown function; identified by SAGE analysis
0.086 6.22E-7 YJL076W NET1 het Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication
0.085 8.15E-7 YPL205C_d YPL205C_d hom Hypothetical protein; deletion of locus affects telomere length
0.084 1.24E-6 YLR061W RPL22A hom Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication
0.083 1.50E-6 YPR175W DPB2 het Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate
0.082 1.82E-6 YJL052W TDH1 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress