YPR133C / SPN1

Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPR133C / SPN1

Click on Significant Values for Screen Details ID:SGTC_67|Compound:0335-0847|FD-Score:4.11|P-value:7.54E-4 ID:SGTC_97|Compound:0535-0701|FD-Score:-3.98|P-value:8.35E-4 ID:SGTC_104|Compound:0074-0074|FD-Score:4.75|P-value:1.20E-4 ID:SGTC_258|Compound:haloperidol|FD-Score:4.50|P-value:2.50E-4 ID:SGTC_262|Compound:fenpropimorph|FD-Score:6.85|P-value:5.30E-8 ID:SGTC_287|Compound:k060-0039|FD-Score:4.42|P-value:3.19E-4 ID:SGTC_302|Compound:1094-0063|FD-Score:4.86|P-value:8.63E-5 ID:SGTC_309|Compound:k408-0051|FD-Score:9.99|P-value:4.13E-15 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:4.63|P-value:1.72E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-7.52|P-value:1.80E-9 ID:SGTC_500|Compound:h-89|FD-Score:-4.36|P-value:2.96E-4 ID:SGTC_584|Compound:0986-0246|FD-Score:4.71|P-value:1.35E-4 ID:SGTC_775|Compound:4577-0580|FD-Score:5.00|P-value:5.46E-5 ID:SGTC_778|Compound:3456-2434|FD-Score:-9.70|P-value:1.43E-14 ID:SGTC_795|Compound:0685-0265|FD-Score:4.44|P-value:2.97E-4 ID:SGTC_851|Compound:0394-0702|FD-Score:4.17|P-value:6.36E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:4.22|P-value:5.61E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:5.51|P-value:1.01E-5 ID:SGTC_969|Compound:1313-0238|FD-Score:5.26|P-value:2.37E-5 ID:SGTC_1269|Compound:0831-0796|FD-Score:-4.00|P-value:7.98E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:6.35|P-value:4.22E-7 ID:SGTC_1520|Compound:flindersine|FD-Score:4.49|P-value:2.63E-4 ID:SGTC_1619|Compound:st003326|FD-Score:-5.57|P-value:5.90E-6 ID:SGTC_1718|Compound:roemerine|FD-Score:6.36|P-value:4.17E-7 ID:SGTC_1732|Compound:st034308|FD-Score:4.39|P-value:3.43E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:6.29|P-value:5.36E-7 ID:SGTC_1786|Compound:berberine|FD-Score:-5.86|P-value:2.03E-6 ID:SGTC_1855|Compound:st056195|FD-Score:-3.93|P-value:9.50E-4 ID:SGTC_1884|Compound:5633444|FD-Score:-4.22|P-value:4.38E-4 ID:SGTC_1927|Compound:st060202|FD-Score:-4.75|P-value:9.15E-5 ID:SGTC_1953|Compound:st076800|FD-Score:4.21|P-value:5.68E-4 ID:SGTC_2021|Compound:4019055|FD-Score:-4.18|P-value:4.88E-4 ID:SGTC_2038|Compound:4100038|FD-Score:-4.00|P-value:7.95E-4 ID:SGTC_2049|Compound:5262831|FD-Score:-9.89|P-value:4.44E-15 ID:SGTC_2053|Compound:5265679|FD-Score:-5.58|P-value:5.71E-6 ID:SGTC_2057|Compound:5236571|FD-Score:-12.70|P-value:8.92E-24 ID:SGTC_2201|Compound:6605497|FD-Score:-4.01|P-value:7.75E-4 ID:SGTC_2296|Compound:7971656|FD-Score:5.31|P-value:1.95E-5 ID:SGTC_2330|Compound:7979373|FD-Score:-5.76|P-value:2.90E-6 ID:SGTC_2337|Compound:9009472|FD-Score:8.39|P-value:3.54E-11 ID:SGTC_2338|Compound:7999974|FD-Score:-4.17|P-value:5.03E-4 ID:SGTC_2415|Compound:st077333|FD-Score:-3.93|P-value:9.63E-4 ID:SGTC_2438|Compound:5259590|FD-Score:5.35|P-value:1.70E-5 ID:SGTC_2459|Compound:5811157|FD-Score:4.17|P-value:6.40E-4 ID:SGTC_2471|Compound:5466956|FD-Score:11.80|P-value:3.13E-20 ID:SGTC_2475|Compound:5812728|FD-Score:7.62|P-value:1.64E-9 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:4.83|P-value:9.30E-5 ID:SGTC_2576|Compound:guaiol|FD-Score:6.36|P-value:4.13E-7 ID:SGTC_2584|Compound:androsterone|FD-Score:-5.13|P-value:2.71E-5 ID:SGTC_2606|Compound:farnesol|FD-Score:4.15|P-value:6.66E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:-4.92|P-value:5.37E-5 ID:SGTC_2719|Compound:novobiocin|FD-Score:4.09|P-value:7.90E-4 ID:SGTC_2724|Compound:maprotiline|FD-Score:-4.94|P-value:5.04E-5 ID:SGTC_2725|Compound:haloperidol|FD-Score:4.46|P-value:2.82E-4 ID:SGTC_2733|Compound:amorolfine|FD-Score:9.32|P-value:2.15E-13 ID:SGTC_2785|Compound:7756463|FD-Score:4.09|P-value:7.85E-4 ID:SGTC_2798|Compound:7813637|FD-Score:5.38|P-value:1.55E-5 ID:SGTC_2799|Compound:7812892|FD-Score:7.61|P-value:1.79E-9 ID:SGTC_2802|Compound:7925815|FD-Score:4.59|P-value:1.96E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-4.63|P-value:1.31E-4 ID:SGTC_2926|Compound:9010726|FD-Score:-6.81|P-value:4.42E-8 ID:SGTC_2968|Compound:9090663|FD-Score:5.42|P-value:1.34E-5 ID:SGTC_2980|Compound:9014342|FD-Score:7.52|P-value:2.64E-9 ID:SGTC_2986|Compound:9022445|FD-Score:-6.36|P-value:2.90E-7 ID:SGTC_2991|Compound:9074669|FD-Score:-4.47|P-value:2.15E-4 ID:SGTC_3018|Compound:9082602|FD-Score:-4.22|P-value:4.30E-4 ID:SGTC_3085|Compound:9117376|FD-Score:5.59|P-value:7.41E-6 ID:SGTC_3217|Compound:9129673|FD-Score:4.04|P-value:9.10E-4 ID:SGTC_3219|Compound:9131112|FD-Score:-4.84|P-value:6.79E-5 ID:SGTC_67|Compound:0335-0847|FD-Score:4.11|P-value:7.54E-4 ID:SGTC_97|Compound:0535-0701|FD-Score:-3.98|P-value:8.35E-4 ID:SGTC_104|Compound:0074-0074|FD-Score:4.75|P-value:1.20E-4 ID:SGTC_258|Compound:haloperidol|FD-Score:4.50|P-value:2.50E-4 ID:SGTC_262|Compound:fenpropimorph|FD-Score:6.85|P-value:5.30E-8 ID:SGTC_287|Compound:k060-0039|FD-Score:4.42|P-value:3.19E-4 ID:SGTC_302|Compound:1094-0063|FD-Score:4.86|P-value:8.63E-5 ID:SGTC_309|Compound:k408-0051|FD-Score:9.99|P-value:4.13E-15 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:4.63|P-value:1.72E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-7.52|P-value:1.80E-9 ID:SGTC_500|Compound:h-89|FD-Score:-4.36|P-value:2.96E-4 ID:SGTC_584|Compound:0986-0246|FD-Score:4.71|P-value:1.35E-4 ID:SGTC_775|Compound:4577-0580|FD-Score:5.00|P-value:5.46E-5 ID:SGTC_778|Compound:3456-2434|FD-Score:-9.70|P-value:1.43E-14 ID:SGTC_795|Compound:0685-0265|FD-Score:4.44|P-value:2.97E-4 ID:SGTC_851|Compound:0394-0702|FD-Score:4.17|P-value:6.36E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:4.22|P-value:5.61E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:5.51|P-value:1.01E-5 ID:SGTC_969|Compound:1313-0238|FD-Score:5.26|P-value:2.37E-5 ID:SGTC_1269|Compound:0831-0796|FD-Score:-4.00|P-value:7.98E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:6.35|P-value:4.22E-7 ID:SGTC_1520|Compound:flindersine|FD-Score:4.49|P-value:2.63E-4 ID:SGTC_1619|Compound:st003326|FD-Score:-5.57|P-value:5.90E-6 ID:SGTC_1718|Compound:roemerine|FD-Score:6.36|P-value:4.17E-7 ID:SGTC_1732|Compound:st034308|FD-Score:4.39|P-value:3.43E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:6.29|P-value:5.36E-7 ID:SGTC_1786|Compound:berberine|FD-Score:-5.86|P-value:2.03E-6 ID:SGTC_1855|Compound:st056195|FD-Score:-3.93|P-value:9.50E-4 ID:SGTC_1884|Compound:5633444|FD-Score:-4.22|P-value:4.38E-4 ID:SGTC_1927|Compound:st060202|FD-Score:-4.75|P-value:9.15E-5 ID:SGTC_1953|Compound:st076800|FD-Score:4.21|P-value:5.68E-4 ID:SGTC_2021|Compound:4019055|FD-Score:-4.18|P-value:4.88E-4 ID:SGTC_2038|Compound:4100038|FD-Score:-4.00|P-value:7.95E-4 ID:SGTC_2049|Compound:5262831|FD-Score:-9.89|P-value:4.44E-15 ID:SGTC_2053|Compound:5265679|FD-Score:-5.58|P-value:5.71E-6 ID:SGTC_2057|Compound:5236571|FD-Score:-12.70|P-value:8.92E-24 ID:SGTC_2201|Compound:6605497|FD-Score:-4.01|P-value:7.75E-4 ID:SGTC_2296|Compound:7971656|FD-Score:5.31|P-value:1.95E-5 ID:SGTC_2330|Compound:7979373|FD-Score:-5.76|P-value:2.90E-6 ID:SGTC_2337|Compound:9009472|FD-Score:8.39|P-value:3.54E-11 ID:SGTC_2338|Compound:7999974|FD-Score:-4.17|P-value:5.03E-4 ID:SGTC_2415|Compound:st077333|FD-Score:-3.93|P-value:9.63E-4 ID:SGTC_2438|Compound:5259590|FD-Score:5.35|P-value:1.70E-5 ID:SGTC_2459|Compound:5811157|FD-Score:4.17|P-value:6.40E-4 ID:SGTC_2471|Compound:5466956|FD-Score:11.80|P-value:3.13E-20 ID:SGTC_2475|Compound:5812728|FD-Score:7.62|P-value:1.64E-9 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:4.83|P-value:9.30E-5 ID:SGTC_2576|Compound:guaiol|FD-Score:6.36|P-value:4.13E-7 ID:SGTC_2584|Compound:androsterone|FD-Score:-5.13|P-value:2.71E-5 ID:SGTC_2606|Compound:farnesol|FD-Score:4.15|P-value:6.66E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:-4.92|P-value:5.37E-5 ID:SGTC_2719|Compound:novobiocin|FD-Score:4.09|P-value:7.90E-4 ID:SGTC_2724|Compound:maprotiline|FD-Score:-4.94|P-value:5.04E-5 ID:SGTC_2725|Compound:haloperidol|FD-Score:4.46|P-value:2.82E-4 ID:SGTC_2733|Compound:amorolfine|FD-Score:9.32|P-value:2.15E-13 ID:SGTC_2785|Compound:7756463|FD-Score:4.09|P-value:7.85E-4 ID:SGTC_2798|Compound:7813637|FD-Score:5.38|P-value:1.55E-5 ID:SGTC_2799|Compound:7812892|FD-Score:7.61|P-value:1.79E-9 ID:SGTC_2802|Compound:7925815|FD-Score:4.59|P-value:1.96E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-4.63|P-value:1.31E-4 ID:SGTC_2926|Compound:9010726|FD-Score:-6.81|P-value:4.42E-8 ID:SGTC_2968|Compound:9090663|FD-Score:5.42|P-value:1.34E-5 ID:SGTC_2980|Compound:9014342|FD-Score:7.52|P-value:2.64E-9 ID:SGTC_2986|Compound:9022445|FD-Score:-6.36|P-value:2.90E-7 ID:SGTC_2991|Compound:9074669|FD-Score:-4.47|P-value:2.15E-4 ID:SGTC_3018|Compound:9082602|FD-Score:-4.22|P-value:4.30E-4 ID:SGTC_3085|Compound:9117376|FD-Score:5.59|P-value:7.41E-6 ID:SGTC_3217|Compound:9129673|FD-Score:4.04|P-value:9.10E-4 ID:SGTC_3219|Compound:9131112|FD-Score:-4.84|P-value:6.79E-5

Top fitness defect scores for YPR133C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2471 5466956 11.80 24.8 µM 3.13E-20 mitochondrial processes 3 3.48E-32 1.04
2 SGTC_309 k408-0051 9.99 1.5 µM 4.13E-15 2 8.56E-24 1.35
3 SGTC_2733 amorolfine 9.32 100.0 µM 2.15E-13 plasma membrane duress 2 5.55E-21 2.34
4 SGTC_2337 9009472 8.39 200.0 µM 3.54E-11 1 2.35E-17 2.68
5 SGTC_2475 5812728 7.62 15.0 µM 1.64E-9 mitochondrial processes 6 1.24E-14 1.83
6 SGTC_2799 7812892 7.61 81.8 µM 1.79E-9 superoxide 4 1.42E-14 0.93
7 SGTC_2980 9014342 7.52 25.3 µM 2.64E-9 6 2.69E-14 1.10
8 SGTC_262 fenpropimorph 6.85 62.5 µM 5.30E-8 ergosterol depletion effects on membrane 5 3.60E-12 0.84
9 SGTC_2576 guaiol 6.36 23.1 µM 4.13E-7 1 1.02E-10 0.94
10 SGTC_1718 roemerine 6.36 11.9 µM 4.17E-7 redox potentiating 7 1.04E-10 0.54
11 SGTC_1271 0832-2808 6.35 169.0 µM 4.22E-7 3 1.06E-10 1.70
12 SGTC_1785 bathophenanthroline 6.29 24.0 µM 5.36E-7 heme requiring 7 1.57E-10 0.28
13 SGTC_3085 9117376 5.59 49.5 µM 7.41E-6 2 1.12E-8 0.16
14 SGTC_947 3447-0010 5.51 211.0 µM 1.01E-5 1 1.84E-8 1.50
15 SGTC_2968 9090663 5.42 33.4 µM 1.34E-5 5 2.95E-8 0.13
16 SGTC_2798 7813637 5.38 45.5 µM 1.55E-5 5 3.73E-8 0.34
17 SGTC_2438 5259590 5.35 200.0 µM 1.70E-5 2 4.31E-8 0.74
18 SGTC_2296 7971656 5.31 126.5 µM 1.95E-5 5 5.41E-8 0.27
19 SGTC_969 1313-0238 5.26 134.0 µM 2.37E-5 mitochondrial processes 9 7.38E-8 0.15
20 SGTC_775 4577-0580 5.00 57.3 µM 5.46E-5 2 2.86E-7 0.89

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.204 8.80E-33 YGR114C_d YGR114C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
0.170 3.82E-23 YMR113W FOL3 het Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis
0.169 6.33E-23 YPL266W DIM1 het Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing
0.167 2.16E-22 YGR116W SPT6 het Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region
0.126 2.31E-13 YJL153C INO1 hom Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element
0.125 4.18E-13 YDL217C TIM22 het Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported
0.118 8.36E-12 YBR192W RIM2 het Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
0.100 5.71E-9 YOL040C RPS15 het Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
0.100 5.90E-9 YOL053W AIM39 hom Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss
0.099 8.27E-9 YOR048C RAT1 het Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination
0.099 1.09E-8 YNL110C NOP15 het Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
0.097 1.62E-8 YIL142W CCT2 het Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
0.097 1.67E-8 YIL110W HPM1 hom AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance
0.095 3.84E-8 YDR188W CCT6 het Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif
0.093 6.96E-8 YMR203W TOM40 het Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore