YPR159W / KRE6

Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p

Zygosity: Homozygous strain
fixedexpanded
Profile for YPR159W / KRE6

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:3.76|P-value:4.79E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:5.84|P-value:1.41E-7 ID:SGTC_71|Compound:0417-1750|FD-Score:3.59|P-value:8.18E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:-4.18|P-value:1.04E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-4.55|P-value:2.76E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.01|P-value:1.87E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.96|P-value:2.23E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:5.25|P-value:1.94E-6 ID:SGTC_686|Compound:1319-0111|FD-Score:-3.52|P-value:8.85E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:5.16|P-value:2.88E-6 ID:SGTC_831|Compound:k061-1017|FD-Score:5.37|P-value:1.18E-6 ID:SGTC_1173|Compound:0443-0020|FD-Score:3.63|P-value:7.11E-4 ID:SGTC_1405|Compound:3909-7894|FD-Score:3.77|P-value:4.59E-4 ID:SGTC_1406|Compound:3909-7906|FD-Score:4.00|P-value:2.20E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:4.00|P-value:2.21E-4 ID:SGTC_1543|Compound:azetidyl-2-carboxylic acid|FD-Score:4.23|P-value:1.02E-4 ID:SGTC_1642|Compound:st009582|FD-Score:-3.50|P-value:9.62E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:5.37|P-value:1.18E-6 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.53|P-value:9.73E-4 ID:SGTC_2200|Compound:6600604|FD-Score:3.85|P-value:3.58E-4 ID:SGTC_2247|Compound:cerulenin|FD-Score:4.24|P-value:9.61E-5 ID:SGTC_2289|Compound:7946404|FD-Score:4.74|P-value:1.56E-5 ID:SGTC_2453|Compound:5731132|FD-Score:4.02|P-value:2.06E-4 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:4.36|P-value:6.44E-5 ID:SGTC_2525|Compound:harmine|FD-Score:-3.71|P-value:4.95E-4 ID:SGTC_2557|Compound:derrustone|FD-Score:4.48|P-value:4.13E-5 ID:SGTC_2982|Compound:9019404|FD-Score:-3.81|P-value:3.64E-4 ID:SGTC_3086|Compound:9117350|FD-Score:-4.26|P-value:8.07E-5 ID:SGTC_3325|Compound:9138760|FD-Score:6.29|P-value:1.59E-8 ID:SGTC_3326|Compound:9140064|FD-Score:6.41|P-value:8.70E-9 ID:SGTC_3331|Compound:9141060|FD-Score:6.01|P-value:6.23E-8 ID:SGTC_3332|Compound:9141069|FD-Score:-5.99|P-value:5.78E-8 ID:SGTC_58|Compound:0957-0393|FD-Score:3.76|P-value:4.79E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:5.84|P-value:1.41E-7 ID:SGTC_71|Compound:0417-1750|FD-Score:3.59|P-value:8.18E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:-4.18|P-value:1.04E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-4.55|P-value:2.76E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.01|P-value:1.87E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.96|P-value:2.23E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:5.25|P-value:1.94E-6 ID:SGTC_686|Compound:1319-0111|FD-Score:-3.52|P-value:8.85E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:5.16|P-value:2.88E-6 ID:SGTC_831|Compound:k061-1017|FD-Score:5.37|P-value:1.18E-6 ID:SGTC_1173|Compound:0443-0020|FD-Score:3.63|P-value:7.11E-4 ID:SGTC_1405|Compound:3909-7894|FD-Score:3.77|P-value:4.59E-4 ID:SGTC_1406|Compound:3909-7906|FD-Score:4.00|P-value:2.20E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:4.00|P-value:2.21E-4 ID:SGTC_1543|Compound:azetidyl-2-carboxylic acid|FD-Score:4.23|P-value:1.02E-4 ID:SGTC_1642|Compound:st009582|FD-Score:-3.50|P-value:9.62E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:5.37|P-value:1.18E-6 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.53|P-value:9.73E-4 ID:SGTC_2200|Compound:6600604|FD-Score:3.85|P-value:3.58E-4 ID:SGTC_2247|Compound:cerulenin|FD-Score:4.24|P-value:9.61E-5 ID:SGTC_2289|Compound:7946404|FD-Score:4.74|P-value:1.56E-5 ID:SGTC_2453|Compound:5731132|FD-Score:4.02|P-value:2.06E-4 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:4.36|P-value:6.44E-5 ID:SGTC_2525|Compound:harmine|FD-Score:-3.71|P-value:4.95E-4 ID:SGTC_2557|Compound:derrustone|FD-Score:4.48|P-value:4.13E-5 ID:SGTC_2982|Compound:9019404|FD-Score:-3.81|P-value:3.64E-4 ID:SGTC_3086|Compound:9117350|FD-Score:-4.26|P-value:8.07E-5 ID:SGTC_3325|Compound:9138760|FD-Score:6.29|P-value:1.59E-8 ID:SGTC_3326|Compound:9140064|FD-Score:6.41|P-value:8.70E-9 ID:SGTC_3331|Compound:9141060|FD-Score:6.01|P-value:6.23E-8 ID:SGTC_3332|Compound:9141069|FD-Score:-5.99|P-value:5.78E-8

Top fitness defect scores for YPR159W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3326 9140064 6.41 16.4 µM 8.70E-9 5 7.47E-11
2 SGTC_3325 9138760 6.29 55.2 µM 1.59E-8 Golgi 6 1.62E-10
3 SGTC_3331 9141060 6.01 2.0 µM 6.23E-8 cell wall 11 9.27E-10
4 SGTC_65 0798-0905 5.84 35.0 µM 1.41E-7 calcium & mitochondrial duress 34 2.63E-9
5 SGTC_831 k061-1017 5.37 217.0 µM 1.18E-6 translation 17 3.97E-8
6 SGTC_1873 rapamycin 5.37 2.0 nM 1.18E-6 41 3.99E-8
7 SGTC_625 k213-0058 5.25 121.0 µM 1.94E-6 translation 52 7.52E-8
8 SGTC_830 4076-0286 5.16 57.8 µM 2.88E-6 translation 29 1.25E-7
9 SGTC_2289 7946404 4.74 165.1 µM 1.56E-5 67 1.08E-6
10 SGTC_2557 derrustone 4.48 83.8 µM 4.13E-5 20 3.73E-6
11 SGTC_2509 methyl 7-deshydroxypyrogallin-4-carboxylate 4.36 54.6 µM 6.44E-5 RPP1 & pyrimidine depletion 23 6.57E-6
12 SGTC_2247 cerulenin 4.24 830.0 nM 9.61E-5 12 1.09E-5
13 SGTC_1543 azetidyl-2-carboxylic acid 4.23 198.0 µM 1.02E-4 8 1.18E-5
14 SGTC_2453 5731132 4.02 22.2 µM 2.06E-4 18 2.88E-5
15 SGTC_1406 3909-7906 4.00 11.9 µM 2.20E-4 60S ribosome export 9 3.14E-5
16 SGTC_1447 4239-0816 4.00 81.9 µM 2.21E-4 26 3.16E-5
17 SGTC_2200 6600604 3.85 21.9 µM 3.58E-4 tubulin folding & SWR complex 55 5.83E-5
18 SGTC_1405 3909-7894 3.77 12.0 µM 4.59E-4 60S ribosome export 13 8.00E-5
19 SGTC_58 0957-0393 3.76 9.0 µM 4.79E-4 cell wall 391 8.44E-5
20 SGTC_1173 0443-0020 3.63 122.0 µM 7.11E-4 TSC3-RPN4 50 1.39E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.183 1.04E-26 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.161 6.52E-21 YDR140W MTQ2 hom S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
0.130 5.04E-14 YBR260C RGD1 hom GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.129 7.47E-14 YGR106C VOA1 hom Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval
0.123 1.06E-12 YAL058W CNE1 hom Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
0.122 1.24E-12 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.122 1.40E-12 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.121 2.12E-12 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.120 2.58E-12 YGR160W_d YGR160W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.120 3.68E-12 YNL322C KRE1 hom Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
0.119 4.41E-12 YPL178W CBC2 hom Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif
0.116 1.56E-11 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.116 1.67E-11 YKR042W UTH1 hom Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication
0.115 2.57E-11 YBR169C SSE2 hom Member of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication
0.114 3.48E-11 YDR363W-A SEM1 hom Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress