YPR167C / MET16

3'-phosphoadenylsulfate reductase, reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism

Zygosity: Homozygous strain
fixedexpanded
Profile for YPR167C / MET16

Click on Significant Values for Screen Details ID:SGTC_285|Compound:1486-1325|FD-Score:-3.17|P-value:5.58E-4 ID:SGTC_293|Compound:4049-0273|FD-Score:3.10|P-value:7.63E-4 ID:SGTC_476|Compound:mead acid|FD-Score:3.18|P-value:5.58E-4 ID:SGTC_557|Compound:1315-0110|FD-Score:3.39|P-value:2.60E-4 ID:SGTC_848|Compound:0368-0089|FD-Score:4.43|P-value:2.89E-6 ID:SGTC_1258|Compound:0671-0043|FD-Score:3.58|P-value:1.21E-4 ID:SGTC_1458|Compound:k015-0034|FD-Score:3.32|P-value:3.39E-4 ID:SGTC_1523|Compound:st012844|FD-Score:-3.19|P-value:5.13E-4 ID:SGTC_1594|Compound:piperine|FD-Score:3.52|P-value:1.58E-4 ID:SGTC_1756|Compound:st041558|FD-Score:3.83|P-value:4.29E-5 ID:SGTC_1766|Compound:st045414|FD-Score:3.25|P-value:4.31E-4 ID:SGTC_1771|Compound:st048882|FD-Score:3.09|P-value:7.84E-4 ID:SGTC_2224|Compound:6650123|FD-Score:3.28|P-value:3.91E-4 ID:SGTC_2762|Compound:leflunomide|FD-Score:3.23|P-value:4.70E-4 ID:SGTC_2830|Compound:7992007|FD-Score:3.22|P-value:4.96E-4 ID:SGTC_2900|Compound:5328528|FD-Score:-3.57|P-value:1.20E-4 ID:SGTC_2902|Compound:5787006|FD-Score:-3.78|P-value:5.09E-5 ID:SGTC_2910|Compound:7948405|FD-Score:-3.93|P-value:2.65E-5 ID:SGTC_2914|Compound:7968218|FD-Score:-3.70|P-value:7.25E-5 ID:SGTC_2915|Compound:7971052|FD-Score:-3.50|P-value:1.60E-4 ID:SGTC_3089|Compound:9117425|FD-Score:3.06|P-value:8.55E-4 ID:SGTC_3095|Compound:9117207|FD-Score:-3.36|P-value:2.75E-4 ID:SGTC_3111|Compound:9122088|FD-Score:-3.61|P-value:1.03E-4 ID:SGTC_3119|Compound:9125183|FD-Score:-3.13|P-value:6.44E-4 ID:SGTC_3170|Compound:9103054|FD-Score:3.40|P-value:2.48E-4 ID:SGTC_285|Compound:1486-1325|FD-Score:-3.17|P-value:5.58E-4 ID:SGTC_293|Compound:4049-0273|FD-Score:3.10|P-value:7.63E-4 ID:SGTC_476|Compound:mead acid|FD-Score:3.18|P-value:5.58E-4 ID:SGTC_557|Compound:1315-0110|FD-Score:3.39|P-value:2.60E-4 ID:SGTC_848|Compound:0368-0089|FD-Score:4.43|P-value:2.89E-6 ID:SGTC_1258|Compound:0671-0043|FD-Score:3.58|P-value:1.21E-4 ID:SGTC_1458|Compound:k015-0034|FD-Score:3.32|P-value:3.39E-4 ID:SGTC_1523|Compound:st012844|FD-Score:-3.19|P-value:5.13E-4 ID:SGTC_1594|Compound:piperine|FD-Score:3.52|P-value:1.58E-4 ID:SGTC_1756|Compound:st041558|FD-Score:3.83|P-value:4.29E-5 ID:SGTC_1766|Compound:st045414|FD-Score:3.25|P-value:4.31E-4 ID:SGTC_1771|Compound:st048882|FD-Score:3.09|P-value:7.84E-4 ID:SGTC_2224|Compound:6650123|FD-Score:3.28|P-value:3.91E-4 ID:SGTC_2762|Compound:leflunomide|FD-Score:3.23|P-value:4.70E-4 ID:SGTC_2830|Compound:7992007|FD-Score:3.22|P-value:4.96E-4 ID:SGTC_2900|Compound:5328528|FD-Score:-3.57|P-value:1.20E-4 ID:SGTC_2902|Compound:5787006|FD-Score:-3.78|P-value:5.09E-5 ID:SGTC_2910|Compound:7948405|FD-Score:-3.93|P-value:2.65E-5 ID:SGTC_2914|Compound:7968218|FD-Score:-3.70|P-value:7.25E-5 ID:SGTC_2915|Compound:7971052|FD-Score:-3.50|P-value:1.60E-4 ID:SGTC_3089|Compound:9117425|FD-Score:3.06|P-value:8.55E-4 ID:SGTC_3095|Compound:9117207|FD-Score:-3.36|P-value:2.75E-4 ID:SGTC_3111|Compound:9122088|FD-Score:-3.61|P-value:1.03E-4 ID:SGTC_3119|Compound:9125183|FD-Score:-3.13|P-value:6.44E-4 ID:SGTC_3170|Compound:9103054|FD-Score:3.40|P-value:2.48E-4

Top fitness defect scores for YPR167C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_848 0368-0089 4.43 85.6 µM 2.89E-6 iron homeostasis 6 4.79E-6
2 SGTC_1756 st041558 3.83 35.5 µM 4.29E-5 14 6.28E-5
3 SGTC_1258 0671-0043 3.58 57.1 µM 1.21E-4 39 1.69E-4
4 SGTC_1594 piperine 3.52 70.1 µM 1.58E-4 25 2.19E-4
5 SGTC_3170 9103054 3.40 49.5 µM 2.48E-4 60S ribosome export 34 3.36E-4
6 SGTC_557 1315-0110 3.39 16.2 µM 2.60E-4 Golgi 39 3.51E-4
7 SGTC_1458 k015-0034 3.32 6.3 µM 3.39E-4 27 4.51E-4
8 SGTC_2224 6650123 3.28 117.7 µM 3.91E-4 10 5.18E-4
9 SGTC_1766 st045414 3.25 44.8 µM 4.31E-4 RPP1 & pyrimidine depletion 60 5.68E-4
10 SGTC_2762 leflunomide 3.23 42.1 µM 4.70E-4 58 6.18E-4
11 SGTC_2830 7992007 3.22 71.4 µM 4.96E-4 42 6.49E-4
12 SGTC_476 mead acid 3.18 10.0 µM 5.58E-4 13 7.28E-4
13 SGTC_293 4049-0273 3.10 38.5 µM 7.63E-4 43 9.80E-4
14 SGTC_1771 st048882 3.09 4.2 µM 7.84E-4 RPP1 & pyrimidine depletion 60 0.00101
15 SGTC_3089 9117425 3.06 49.5 µM 8.55E-4 68 0.00109
16 SGTC_299 1273-0059 3.00 1.4 µM 0.00106 55 0.00134
17 SGTC_2222 6885208 2.98 172.1 µM 0.00115 11 0.00145
18 SGTC_2165 5727197 2.95 32.1 µM 0.00125 100 0.00157
19 SGTC_2228 7354201 2.95 200.0 µM 0.00127 endomembrane recycling 16 0.00159
20 SGTC_2694 st077685 2.95 81.9 µM 0.00127 endomembrane recycling 81 0.00159

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.176 1.01E-24 YCL074W YCL074W hom Pseudogene: encodes fragment of Ty Pol protein
0.094 5.41E-8 YLR237W THI7 hom Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia
0.088 3.55E-7 YAR020C PAU7 hom Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme
0.080 3.15E-6 YPL258C THI21 hom Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p
0.077 9.09E-6 YDR153C ENT5 hom Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin
0.076 1.10E-5 YNR073C_p YNR073C_p hom Putative mannitol dehydrogenase; identical in amino acid sequence to Dsf1p
0.071 3.54E-5 YOL103W ITR2 hom Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively
0.071 3.72E-5 YGR121C MEP1 hom Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation
0.071 4.14E-5 YFL014W HSP12 hom Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
0.070 4.84E-5 YGR110W CLD1 hom Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58
0.070 4.87E-5 YOL077C BRX1 het Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif
0.069 6.56E-5 YMR061W RNA14 het Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
0.068 7.93E-5 YGR249W MGA1 hom Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants
0.067 1.01E-4 YOR208W PTP2 hom Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the nucleus
0.067 1.02E-4 YKL205W LOS1 hom Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm