YPR168W / NUT2

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPR168W / NUT2

Click on Significant Values for Screen Details ID:SGTC_11|Compound:0438-0307|FD-Score:-3.51|P-value:6.40E-5 ID:SGTC_36|Compound:0335-0881|FD-Score:-3.24|P-value:2.03E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:4.16|P-value:2.46E-6 ID:SGTC_145|Compound:3737-0113|FD-Score:4.12|P-value:3.06E-6 ID:SGTC_174|Compound:3553-0389|FD-Score:-3.52|P-value:6.29E-5 ID:SGTC_184|Compound:1494-0393|FD-Score:3.13|P-value:2.96E-4 ID:SGTC_203|Compound:k066-0004|FD-Score:3.46|P-value:7.37E-5 ID:SGTC_234|Compound:nsc-17383|FD-Score:3.46|P-value:7.19E-5 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.11|P-value:3.12E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:4.03|P-value:4.94E-6 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:2.83|P-value:9.48E-4 ID:SGTC_520|Compound:3584-0023|FD-Score:-3.45|P-value:8.48E-5 ID:SGTC_540|Compound:1187-0821|FD-Score:3.23|P-value:1.95E-4 ID:SGTC_645|Compound:0845-0943|FD-Score:4.17|P-value:2.42E-6 ID:SGTC_744|Compound:1594-0068|FD-Score:3.58|P-value:4.34E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.76|P-value:2.08E-5 ID:SGTC_752|Compound:1896-0090|FD-Score:-3.49|P-value:7.10E-5 ID:SGTC_797|Compound:k781-0471|FD-Score:3.26|P-value:1.71E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:3.14|P-value:2.86E-4 ID:SGTC_939|Compound:1165-0479|FD-Score:2.99|P-value:5.17E-4 ID:SGTC_941|Compound:3237-0776|FD-Score:2.89|P-value:7.53E-4 ID:SGTC_981|Compound:1488-1094|FD-Score:-2.90|P-value:7.93E-4 ID:SGTC_1018|Compound:4259-0013|FD-Score:-3.09|P-value:3.85E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:2.96|P-value:5.83E-4 ID:SGTC_1067|Compound:clotrimazole|FD-Score:3.02|P-value:4.50E-4 ID:SGTC_1224|Compound:0823-0416|FD-Score:-3.15|P-value:3.02E-4 ID:SGTC_1226|Compound:3909-7979|FD-Score:-3.63|P-value:3.73E-5 ID:SGTC_1340|Compound:1486-1359|FD-Score:3.24|P-value:1.87E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:3.09|P-value:3.45E-4 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:3.59|P-value:4.02E-5 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:3.32|P-value:1.34E-4 ID:SGTC_1602|Compound:st001878|FD-Score:2.88|P-value:7.69E-4 ID:SGTC_1755|Compound:st041960|FD-Score:3.09|P-value:3.47E-4 ID:SGTC_1773|Compound:st048542|FD-Score:3.63|P-value:3.43E-5 ID:SGTC_1776|Compound:st049249|FD-Score:3.49|P-value:6.26E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:3.79|P-value:1.56E-5 ID:SGTC_2052|Compound:5265182|FD-Score:5.34|P-value:2.36E-9 ID:SGTC_2055|Compound:5235184|FD-Score:7.17|P-value:2.01E-15 ID:SGTC_2058|Compound:5236698|FD-Score:-4.48|P-value:5.12E-7 ID:SGTC_2198|Compound:6589944|FD-Score:2.83|P-value:9.23E-4 ID:SGTC_2273|Compound:7815444|FD-Score:-3.02|P-value:5.08E-4 ID:SGTC_2291|Compound:7917025|FD-Score:3.03|P-value:4.42E-4 ID:SGTC_2293|Compound:7624732|FD-Score:3.45|P-value:7.53E-5 ID:SGTC_2339|Compound:9000433|FD-Score:-3.32|P-value:1.47E-4 ID:SGTC_2360|Compound:9040895|FD-Score:4.78|P-value:8.06E-8 ID:SGTC_2581|Compound:avocatin a|FD-Score:3.17|P-value:2.49E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:-3.85|P-value:1.32E-5 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:-3.36|P-value:1.23E-4 ID:SGTC_2821|Compound:7996764|FD-Score:-3.05|P-value:4.38E-4 ID:SGTC_2848|Compound:9016370|FD-Score:3.85|P-value:1.19E-5 ID:SGTC_2929|Compound:9013348|FD-Score:-2.97|P-value:6.00E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-4.78|P-value:9.42E-8 ID:SGTC_3304|Compound:9125678|FD-Score:-3.13|P-value:3.24E-4 ID:SGTC_3306|Compound:9127676|FD-Score:-3.53|P-value:6.02E-5 ID:SGTC_3307|Compound:9126885|FD-Score:4.90|P-value:3.82E-8 ID:SGTC_11|Compound:0438-0307|FD-Score:-3.51|P-value:6.40E-5 ID:SGTC_36|Compound:0335-0881|FD-Score:-3.24|P-value:2.03E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:4.16|P-value:2.46E-6 ID:SGTC_145|Compound:3737-0113|FD-Score:4.12|P-value:3.06E-6 ID:SGTC_174|Compound:3553-0389|FD-Score:-3.52|P-value:6.29E-5 ID:SGTC_184|Compound:1494-0393|FD-Score:3.13|P-value:2.96E-4 ID:SGTC_203|Compound:k066-0004|FD-Score:3.46|P-value:7.37E-5 ID:SGTC_234|Compound:nsc-17383|FD-Score:3.46|P-value:7.19E-5 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.11|P-value:3.12E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:4.03|P-value:4.94E-6 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:2.83|P-value:9.48E-4 ID:SGTC_520|Compound:3584-0023|FD-Score:-3.45|P-value:8.48E-5 ID:SGTC_540|Compound:1187-0821|FD-Score:3.23|P-value:1.95E-4 ID:SGTC_645|Compound:0845-0943|FD-Score:4.17|P-value:2.42E-6 ID:SGTC_744|Compound:1594-0068|FD-Score:3.58|P-value:4.34E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.76|P-value:2.08E-5 ID:SGTC_752|Compound:1896-0090|FD-Score:-3.49|P-value:7.10E-5 ID:SGTC_797|Compound:k781-0471|FD-Score:3.26|P-value:1.71E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:3.14|P-value:2.86E-4 ID:SGTC_939|Compound:1165-0479|FD-Score:2.99|P-value:5.17E-4 ID:SGTC_941|Compound:3237-0776|FD-Score:2.89|P-value:7.53E-4 ID:SGTC_981|Compound:1488-1094|FD-Score:-2.90|P-value:7.93E-4 ID:SGTC_1018|Compound:4259-0013|FD-Score:-3.09|P-value:3.85E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:2.96|P-value:5.83E-4 ID:SGTC_1067|Compound:clotrimazole|FD-Score:3.02|P-value:4.50E-4 ID:SGTC_1224|Compound:0823-0416|FD-Score:-3.15|P-value:3.02E-4 ID:SGTC_1226|Compound:3909-7979|FD-Score:-3.63|P-value:3.73E-5 ID:SGTC_1340|Compound:1486-1359|FD-Score:3.24|P-value:1.87E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:3.09|P-value:3.45E-4 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:3.59|P-value:4.02E-5 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:3.32|P-value:1.34E-4 ID:SGTC_1602|Compound:st001878|FD-Score:2.88|P-value:7.69E-4 ID:SGTC_1755|Compound:st041960|FD-Score:3.09|P-value:3.47E-4 ID:SGTC_1773|Compound:st048542|FD-Score:3.63|P-value:3.43E-5 ID:SGTC_1776|Compound:st049249|FD-Score:3.49|P-value:6.26E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:3.79|P-value:1.56E-5 ID:SGTC_2052|Compound:5265182|FD-Score:5.34|P-value:2.36E-9 ID:SGTC_2055|Compound:5235184|FD-Score:7.17|P-value:2.01E-15 ID:SGTC_2058|Compound:5236698|FD-Score:-4.48|P-value:5.12E-7 ID:SGTC_2198|Compound:6589944|FD-Score:2.83|P-value:9.23E-4 ID:SGTC_2273|Compound:7815444|FD-Score:-3.02|P-value:5.08E-4 ID:SGTC_2291|Compound:7917025|FD-Score:3.03|P-value:4.42E-4 ID:SGTC_2293|Compound:7624732|FD-Score:3.45|P-value:7.53E-5 ID:SGTC_2339|Compound:9000433|FD-Score:-3.32|P-value:1.47E-4 ID:SGTC_2360|Compound:9040895|FD-Score:4.78|P-value:8.06E-8 ID:SGTC_2581|Compound:avocatin a|FD-Score:3.17|P-value:2.49E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:-3.85|P-value:1.32E-5 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:-3.36|P-value:1.23E-4 ID:SGTC_2821|Compound:7996764|FD-Score:-3.05|P-value:4.38E-4 ID:SGTC_2848|Compound:9016370|FD-Score:3.85|P-value:1.19E-5 ID:SGTC_2929|Compound:9013348|FD-Score:-2.97|P-value:6.00E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-4.78|P-value:9.42E-8 ID:SGTC_3304|Compound:9125678|FD-Score:-3.13|P-value:3.24E-4 ID:SGTC_3306|Compound:9127676|FD-Score:-3.53|P-value:6.02E-5 ID:SGTC_3307|Compound:9126885|FD-Score:4.90|P-value:3.82E-8

Top fitness defect scores for YPR168W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2055 5235184 7.17 200.0 µM 2.01E-15 ERG2 1 3.81E-13 2.12
2 SGTC_2052 5265182 5.34 7.1 µM 2.36E-9 1 4.59E-8 0.50
3 SGTC_3307 9126885 4.90 69.3 µM 3.82E-8 4 4.75E-7 0.29
4 SGTC_2360 9040895 4.78 200.0 µM 8.06E-8 RPP1 & pyrimidine depletion 5 8.87E-7 0.19
5 SGTC_645 0845-0943 4.17 5.4 µM 2.42E-6 60S ribosome export 5 1.54E-5 0.76
6 SGTC_42 0133-0086 4.16 60.6 µM 2.46E-6 5 1.56E-5 0.01
7 SGTC_145 3737-0113 4.12 38.4 µM 3.06E-6 DNA intercalators 47 1.88E-5 0.09
8 SGTC_245 methotrexate 4.03 289.6 µM 4.94E-6 13 2.81E-5 0.08
9 SGTC_2848 9016370 3.85 26.0 µM 1.19E-5 4 5.86E-5 0.04
10 SGTC_1926 methyl fluorone black 3.79 53.6 µM 1.56E-5 DNA intercalators 30 7.41E-5 0.17
11 SGTC_1773 st048542 3.63 73.6 µM 3.43E-5 31 1.43E-4 0.02
12 SGTC_1592 4',5'-dihydroxyflavone 3.59 8.8 µM 4.02E-5 60S ribosome export 10 1.64E-4 0.58
13 SGTC_744 1594-0068 3.58 283.0 µM 4.34E-5 amide catabolism 7 1.75E-4 0.02
14 SGTC_1776 st049249 3.49 45.9 µM 6.26E-5 13 2.38E-4 0.02
15 SGTC_234 nsc-17383 3.46 10.7 µM 7.19E-5 ERG2 6 2.67E-4 0.01
16 SGTC_203 k066-0004 3.46 30.9 µM 7.37E-5 RSC & ERG11 16 2.73E-4 0.00
17 SGTC_2293 7624732 3.45 200.0 µM 7.53E-5 12 2.78E-4 0.05
18 SGTC_1593 justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 3.32 54.9 µM 1.34E-4 redox potentiating 11 4.52E-4 0.05
19 SGTC_797 k781-0471 3.26 270.0 µM 1.71E-4 RPP1 & pyrimidine depletion 7 5.54E-4 0.02
20 SGTC_1340 1486-1359 3.24 257.0 µM 1.87E-4 12 5.97E-4 1.35E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.140 3.58E-16 YPL035C_d YPL035C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene
0.130 5.15E-14 YER099C PRS2 hom 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication
0.129 5.99E-14 YHR149C SKG6 hom Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.120 2.64E-12 YLR223C IFH1 het Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing
0.119 4.63E-12 YDR534C FIT1 hom Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall
0.115 2.30E-11 YPL197C_d YPL197C_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B
0.113 6.38E-11 YBR072C-A_p YBR072C-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.109 2.11E-10 YOL035C_d YOL035C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.108 2.97E-10 YLR071C RGR1 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation
0.108 3.47E-10 YEL064C AVT2 hom Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
0.107 4.80E-10 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.105 1.05E-9 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.102 2.72E-9 YJR020W_d YJR020W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.101 5.01E-9 YGR179C OKP1 het Outer kinetochore protein required for accurate chromosome segregation; component of COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) a kinetochore sub-complex which functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-Q and fission yeast fta7
0.100 5.69E-9 YGL140C_p YGL140C_p hom Putative protein of unknown function; non-essential gene; contains multiple predicted transmembrane domains