YPR173C / VPS4

AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism

Zygosity: Homozygous strain
fixedexpanded
Profile for YPR173C / VPS4

Click on Significant Values for Screen Details ID:SGTC_21|Compound:0849-0113|FD-Score:3.98|P-value:2.83E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:4.27|P-value:1.10E-4 ID:SGTC_37|Compound:0566-0070|FD-Score:4.84|P-value:1.38E-5 ID:SGTC_40|Compound:0327-0103|FD-Score:-3.60|P-value:8.60E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:3.85|P-value:4.27E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:4.04|P-value:2.32E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:5.09|P-value:5.07E-6 ID:SGTC_137|Compound:0438-0332|FD-Score:6.48|P-value:9.82E-9 ID:SGTC_142|Compound:0335-0849|FD-Score:3.70|P-value:6.79E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:4.48|P-value:5.13E-5 ID:SGTC_159|Compound:3970-0959|FD-Score:4.74|P-value:2.02E-5 ID:SGTC_221|Compound:trifluoperazine|FD-Score:4.98|P-value:8.12E-6 ID:SGTC_232|Compound:clozapine|FD-Score:3.65|P-value:7.93E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:-3.61|P-value:8.45E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:5.86|P-value:1.92E-7 ID:SGTC_271|Compound:rotenone|FD-Score:3.93|P-value:3.36E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:4.78|P-value:1.74E-5 ID:SGTC_341|Compound:1486-1430|FD-Score:4.38|P-value:7.30E-5 ID:SGTC_365|Compound:0121-0052|FD-Score:8.06|P-value:1.43E-12 ID:SGTC_391|Compound:gliotoxin|FD-Score:3.76|P-value:5.74E-4 ID:SGTC_488|Compound:nitrendipine|FD-Score:3.93|P-value:3.29E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:5.32|P-value:2.06E-6 ID:SGTC_525|Compound:k781-2675|FD-Score:3.73|P-value:6.23E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:3.84|P-value:4.40E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:6.48|P-value:9.83E-9 ID:SGTC_619|Compound:1652-1634|FD-Score:4.48|P-value:5.30E-5 ID:SGTC_626|Compound:0214-0009|FD-Score:5.06|P-value:5.93E-6 ID:SGTC_659|Compound:k018-0002|FD-Score:3.99|P-value:2.73E-4 ID:SGTC_670|Compound:4204-0025|FD-Score:4.40|P-value:6.96E-5 ID:SGTC_748|Compound:0136-0228|FD-Score:12.00|P-value:8.25E-26 ID:SGTC_769|Compound:0302-0231|FD-Score:-3.86|P-value:3.88E-4 ID:SGTC_789|Compound:1309-1135|FD-Score:3.71|P-value:6.57E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:6.68|P-value:3.53E-9 ID:SGTC_919|Compound:tnp00227|FD-Score:4.53|P-value:4.38E-5 ID:SGTC_1066|Compound:ketoconazole|FD-Score:5.39|P-value:1.53E-6 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:4.03|P-value:2.38E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:5.74|P-value:3.32E-7 ID:SGTC_1174|Compound:0739-0097|FD-Score:6.73|P-value:2.70E-9 ID:SGTC_1177|Compound:0983-0204|FD-Score:4.28|P-value:1.05E-4 ID:SGTC_1208|Compound:2323-2347|FD-Score:4.02|P-value:2.48E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:-3.58|P-value:9.15E-4 ID:SGTC_1346|Compound:1488-2224|FD-Score:3.86|P-value:4.21E-4 ID:SGTC_1354|Compound:1498-1681|FD-Score:3.80|P-value:5.02E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:14.90|P-value:2.61E-38 ID:SGTC_1658|Compound:st012944|FD-Score:6.40|P-value:1.47E-8 ID:SGTC_1687|Compound:st020778|FD-Score:6.93|P-value:9.61E-10 ID:SGTC_1716|Compound:st033144|FD-Score:-4.16|P-value:1.48E-4 ID:SGTC_1750|Compound:st081859|FD-Score:11.00|P-value:7.13E-22 ID:SGTC_1760|Compound:st045453|FD-Score:3.72|P-value:6.48E-4 ID:SGTC_1773|Compound:st048542|FD-Score:4.07|P-value:2.15E-4 ID:SGTC_1830|Compound:st055982|FD-Score:4.08|P-value:2.07E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:7.65|P-value:1.69E-11 ID:SGTC_1859|Compound:st055998|FD-Score:4.84|P-value:1.35E-5 ID:SGTC_2033|Compound:5192550|FD-Score:3.81|P-value:4.79E-4 ID:SGTC_2322|Compound:7413319|FD-Score:3.86|P-value:4.14E-4 ID:SGTC_2359|Compound:9039622|FD-Score:4.46|P-value:5.62E-5 ID:SGTC_2361|Compound:9034121|FD-Score:-4.43|P-value:5.83E-5 ID:SGTC_2385|Compound:9074692|FD-Score:3.75|P-value:5.90E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:-3.74|P-value:5.78E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:3.59|P-value:9.35E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:12.50|P-value:1.55E-27 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:5.22|P-value:3.10E-6 ID:SGTC_2619|Compound:obliquin|FD-Score:7.65|P-value:1.66E-11 ID:SGTC_2629|Compound:bisabolol|FD-Score:6.53|P-value:7.63E-9 ID:SGTC_2671|Compound:danthron|FD-Score:3.76|P-value:5.61E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:6.53|P-value:7.57E-9 ID:SGTC_2688|Compound:kanamycin b|FD-Score:8.41|P-value:1.57E-13 ID:SGTC_2752|Compound:diclazuril|FD-Score:4.21|P-value:1.33E-4 ID:SGTC_2783|Compound:7738165|FD-Score:4.52|P-value:4.54E-5 ID:SGTC_3058|Compound:9093861|FD-Score:-4.35|P-value:7.68E-5 ID:SGTC_3240|Compound:9133527|FD-Score:-3.66|P-value:7.23E-4 ID:SGTC_21|Compound:0849-0113|FD-Score:3.98|P-value:2.83E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:4.27|P-value:1.10E-4 ID:SGTC_37|Compound:0566-0070|FD-Score:4.84|P-value:1.38E-5 ID:SGTC_40|Compound:0327-0103|FD-Score:-3.60|P-value:8.60E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:3.85|P-value:4.27E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:4.04|P-value:2.32E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:5.09|P-value:5.07E-6 ID:SGTC_137|Compound:0438-0332|FD-Score:6.48|P-value:9.82E-9 ID:SGTC_142|Compound:0335-0849|FD-Score:3.70|P-value:6.79E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:4.48|P-value:5.13E-5 ID:SGTC_159|Compound:3970-0959|FD-Score:4.74|P-value:2.02E-5 ID:SGTC_221|Compound:trifluoperazine|FD-Score:4.98|P-value:8.12E-6 ID:SGTC_232|Compound:clozapine|FD-Score:3.65|P-value:7.93E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:-3.61|P-value:8.45E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:5.86|P-value:1.92E-7 ID:SGTC_271|Compound:rotenone|FD-Score:3.93|P-value:3.36E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:4.78|P-value:1.74E-5 ID:SGTC_341|Compound:1486-1430|FD-Score:4.38|P-value:7.30E-5 ID:SGTC_365|Compound:0121-0052|FD-Score:8.06|P-value:1.43E-12 ID:SGTC_391|Compound:gliotoxin|FD-Score:3.76|P-value:5.74E-4 ID:SGTC_488|Compound:nitrendipine|FD-Score:3.93|P-value:3.29E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:5.32|P-value:2.06E-6 ID:SGTC_525|Compound:k781-2675|FD-Score:3.73|P-value:6.23E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:3.84|P-value:4.40E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:6.48|P-value:9.83E-9 ID:SGTC_619|Compound:1652-1634|FD-Score:4.48|P-value:5.30E-5 ID:SGTC_626|Compound:0214-0009|FD-Score:5.06|P-value:5.93E-6 ID:SGTC_659|Compound:k018-0002|FD-Score:3.99|P-value:2.73E-4 ID:SGTC_670|Compound:4204-0025|FD-Score:4.40|P-value:6.96E-5 ID:SGTC_748|Compound:0136-0228|FD-Score:12.00|P-value:8.25E-26 ID:SGTC_769|Compound:0302-0231|FD-Score:-3.86|P-value:3.88E-4 ID:SGTC_789|Compound:1309-1135|FD-Score:3.71|P-value:6.57E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:6.68|P-value:3.53E-9 ID:SGTC_919|Compound:tnp00227|FD-Score:4.53|P-value:4.38E-5 ID:SGTC_1066|Compound:ketoconazole|FD-Score:5.39|P-value:1.53E-6 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:4.03|P-value:2.38E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:5.74|P-value:3.32E-7 ID:SGTC_1174|Compound:0739-0097|FD-Score:6.73|P-value:2.70E-9 ID:SGTC_1177|Compound:0983-0204|FD-Score:4.28|P-value:1.05E-4 ID:SGTC_1208|Compound:2323-2347|FD-Score:4.02|P-value:2.48E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:-3.58|P-value:9.15E-4 ID:SGTC_1346|Compound:1488-2224|FD-Score:3.86|P-value:4.21E-4 ID:SGTC_1354|Compound:1498-1681|FD-Score:3.80|P-value:5.02E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:14.90|P-value:2.61E-38 ID:SGTC_1658|Compound:st012944|FD-Score:6.40|P-value:1.47E-8 ID:SGTC_1687|Compound:st020778|FD-Score:6.93|P-value:9.61E-10 ID:SGTC_1716|Compound:st033144|FD-Score:-4.16|P-value:1.48E-4 ID:SGTC_1750|Compound:st081859|FD-Score:11.00|P-value:7.13E-22 ID:SGTC_1760|Compound:st045453|FD-Score:3.72|P-value:6.48E-4 ID:SGTC_1773|Compound:st048542|FD-Score:4.07|P-value:2.15E-4 ID:SGTC_1830|Compound:st055982|FD-Score:4.08|P-value:2.07E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:7.65|P-value:1.69E-11 ID:SGTC_1859|Compound:st055998|FD-Score:4.84|P-value:1.35E-5 ID:SGTC_2033|Compound:5192550|FD-Score:3.81|P-value:4.79E-4 ID:SGTC_2322|Compound:7413319|FD-Score:3.86|P-value:4.14E-4 ID:SGTC_2359|Compound:9039622|FD-Score:4.46|P-value:5.62E-5 ID:SGTC_2361|Compound:9034121|FD-Score:-4.43|P-value:5.83E-5 ID:SGTC_2385|Compound:9074692|FD-Score:3.75|P-value:5.90E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:-3.74|P-value:5.78E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:3.59|P-value:9.35E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:12.50|P-value:1.55E-27 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:5.22|P-value:3.10E-6 ID:SGTC_2619|Compound:obliquin|FD-Score:7.65|P-value:1.66E-11 ID:SGTC_2629|Compound:bisabolol|FD-Score:6.53|P-value:7.63E-9 ID:SGTC_2671|Compound:danthron|FD-Score:3.76|P-value:5.61E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:6.53|P-value:7.57E-9 ID:SGTC_2688|Compound:kanamycin b|FD-Score:8.41|P-value:1.57E-13 ID:SGTC_2752|Compound:diclazuril|FD-Score:4.21|P-value:1.33E-4 ID:SGTC_2783|Compound:7738165|FD-Score:4.52|P-value:4.54E-5 ID:SGTC_3058|Compound:9093861|FD-Score:-4.35|P-value:7.68E-5 ID:SGTC_3240|Compound:9133527|FD-Score:-3.66|P-value:7.23E-4

Top fitness defect scores for YPR173C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1593 justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 14.90 54.9 µM 2.61E-38 redox potentiating 6 3.03E-50
2 SGTC_2582 agaric acid 12.50 100.0 µM 1.55E-27 redox potentiating 35 5.87E-36
3 SGTC_748 0136-0228 12.00 77.9 µM 8.25E-26 redox potentiating 7 1.14E-33
4 SGTC_1750 st081859 11.00 2.4 µM 7.13E-22 endomembrane recycling 37 1.89E-28
5 SGTC_2688 kanamycin b 8.41 96.7 µM 1.57E-13 calcium & mitochondrial duress 54 2.12E-17
6 SGTC_365 0121-0052 8.06 13.6 µM 1.43E-12 mitochondrial response to ROS 6 3.93E-16
7 SGTC_2619 obliquin 7.65 87.9 µM 1.66E-11 cell wall 48 1.01E-14
8 SGTC_1844 rapamycin 7.65 4.0 nM 1.69E-11 63 1.03E-14
9 SGTC_1687 st020778 6.93 55.4 µM 9.61E-10 88 2.15E-12
10 SGTC_1174 0739-0097 6.73 51.1 µM 2.70E-9 redox potentiating 4 8.40E-12
11 SGTC_888 0916-0002 6.68 7.0 µM 3.53E-9 NEO1 20 1.20E-11
12 SGTC_2679 rapamycin 6.53 1.0 nM 7.57E-9 67 3.28E-11
13 SGTC_2629 bisabolol 6.53 100.0 µM 7.63E-9 4 3.31E-11
14 SGTC_137 0438-0332 6.48 85.4 µM 9.82E-9 ubiquinone biosynthesis & proteasome 56 4.62E-11
15 SGTC_568 1193-0097 6.48 7.5 µM 9.83E-9 ERAD & cell cycle 54 4.63E-11
16 SGTC_1658 st012944 6.40 20.0 µM 1.47E-8 iron homeostasis 26 7.86E-11
17 SGTC_268 camptothecin 5.86 39.6 µM 1.92E-7 DNA damage response 33 2.33E-9
18 SGTC_1165 k072-0275 5.74 30.3 µM 3.32E-7 12 4.81E-9
19 SGTC_1066 ketoconazole 5.39 628.0 nM 1.53E-6 3 3.61E-8
20 SGTC_524 r073-0010 5.32 27.8 µM 2.06E-6 22 5.32E-8

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.418 3.61E-142 YJR102C VPS25 hom Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome
0.392 5.99E-124 YPL065W VPS28 hom Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p
0.369 7.24E-109 YDR323C PEP7 hom Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
0.357 1.09E-101 YKL041W VPS24 hom One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway
0.350 4.17E-97 YNR006W VPS27 hom Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
0.347 1.97E-95 YLR119W SRN2 hom Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus
0.343 5.35E-93 YMR077C VPS20 hom Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes
0.341 2.73E-92 YPL002C SNF8 hom Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression
0.337 1.29E-89 YLR025W SNF7 hom One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
0.333 7.23E-88 YOR036W PEP12 hom Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
0.317 3.08E-79 YGL212W VAM7 hom Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
0.316 1.58E-78 YKR035C_d OPI8_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A
0.309 5.44E-75 YKL002W DID4 hom Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis
0.306 1.02E-73 YCL008C STP22 hom Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype
0.299 1.97E-70 YPL084W BRO1 hom Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes