YPR174C

Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery; potential Cdc28p substrate; binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study; relative distribution to foci at the nuclear periphery increases upon DNA replication stress; YPR174C has a paralog, NBP1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YPR174C / YPR174C

Click on Significant Values for Screen Details

Top fitness defect scores for YPR174C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_1124 fisetin 5.82 1.2 µM 1.10E-9 7 2.99E-9
2 SGTC_393 acivicin 4.96 6.2 µM 1.70E-7 11 3.59E-7
3 SGTC_1689 st020265 4.78 53.5 µM 4.27E-7 12 8.59E-7
4 SGTC_1123 mangostin 4.63 811.3 nM 9.31E-7 mitochondrial response to ROS 20 1.80E-6
5 SGTC_384 k007-0144 4.62 5.2 µM 9.75E-7 ERAD & cell cycle 42 1.88E-6
6 SGTC_2615 purpurogallin-4-carboxylic acid 4.26 100.0 µM 5.76E-6 redox potentiating 59 1.02E-5
7 SGTC_1294 1000-1494 4.15 117.0 µM 9.58E-6 19 1.65E-5
8 SGTC_1766 st045414 4.06 44.8 µM 1.46E-5 RPP1 & pyrimidine depletion 22 2.46E-5
9 SGTC_1446 4239-0190 3.96 62.0 µM 2.30E-5 37 3.78E-5
10 SGTC_1045 1348-1485 3.96 165.0 µM 2.32E-5 32 3.82E-5
11 SGTC_1287 0971-0001 3.95 34.6 µM 2.43E-5 sphingolipid biosynthesis & PDR1 44 3.98E-5
12 SGTC_367 0335-0881 3.73 7.1 µM 6.10E-5 ubiquinone biosynthesis & proteasome 88 9.55E-5
13 SGTC_771 0250-0039 3.67 27.3 µM 7.78E-5 20 1.20E-4
14 SGTC_2135 5326629 3.67 150.4 µM 7.87E-5 RPP1 & pyrimidine depletion 37 1.22E-4
15 SGTC_2333 9017922 3.58 200.0 µM 1.15E-4 38 1.74E-4
16 SGTC_1593 justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 3.53 54.9 µM 1.39E-4 redox potentiating 70 2.09E-4
17 SGTC_951 1171-0579 3.52 82.7 µM 1.42E-4 cell wall 106 2.14E-4
18 SGTC_2131 toluylene red 3.50 5.3 µM 1.53E-4 60S ribosome export 37 2.29E-4
19 SGTC_1290 0986-0249 3.47 10.2 µM 1.78E-4 DNA intercalators 35 2.64E-4
20 SGTC_2018 5133068 3.46 143.0 µM 1.84E-4 36 2.73E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.159 1.66E-20 YDL207W GLE1 het Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export
0.147 1.16E-17 YMR067C UBX4 hom UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress
0.129 5.49E-14 YCL014W BUD3 hom Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding
0.128 1.01E-13 YPR035W GLN1 het Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.115 2.17E-11 YJR103W URA8 hom Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication
0.114 3.48E-11 YGR197C SNG1 hom Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance
0.111 1.01E-10 YGL126W SCS3 hom Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol
0.110 1.84E-10 YOR382W FIT2 hom Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall
0.107 6.06E-10 YJR129C_p YJR129C_p hom Putative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.105 9.27E-10 YDR266C HEL2 hom RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor
0.104 1.49E-9 YOL147C PEX11 hom Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p
0.103 1.85E-9 YDR279W RNH202 hom Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome
0.103 2.47E-9 YNL128W TEP1 hom PTEN homolog with no demonstrated inositol lipid phosphatase activity; plays a role in normal sporulation; homolog of human tumor suppressor gene PTEN/MMAC1/TEP1 and fission yeast ptn1
0.099 1.03E-8 YFR007W YFH7 hom Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases
0.098 1.15E-8 YHR080C YHR080C hom Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication