YPR175W / DPB2

Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPR175W / DPB2

Click on Significant Values for Screen Details ID:SGTC_35|Compound:0133-0054|FD-Score:2.63|P-value:8.03E-4 ID:SGTC_48|Compound:0382-0018|FD-Score:3.03|P-value:1.39E-4 ID:SGTC_225|Compound:nsc-122657|FD-Score:-3.51|P-value:1.11E-5 ID:SGTC_260|Compound:nsc-65238|FD-Score:-3.15|P-value:6.97E-5 ID:SGTC_401|Compound:neomycin|FD-Score:-3.33|P-value:2.91E-5 ID:SGTC_535|Compound:2293-4129|FD-Score:2.59|P-value:9.51E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:2.78|P-value:4.39E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:2.92|P-value:2.29E-4 ID:SGTC_729|Compound:4092-0585|FD-Score:2.97|P-value:1.87E-4 ID:SGTC_894|Compound:0960-0238|FD-Score:3.12|P-value:9.14E-5 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:-2.57|P-value:9.58E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:-2.80|P-value:3.55E-4 ID:SGTC_1171|Compound:0368-0077|FD-Score:2.78|P-value:4.25E-4 ID:SGTC_1177|Compound:0983-0204|FD-Score:-4.41|P-value:5.20E-8 ID:SGTC_1210|Compound:3013-0140|FD-Score:3.26|P-value:4.65E-5 ID:SGTC_1306|Compound:1185-0270|FD-Score:6.34|P-value:1.36E-14 ID:SGTC_1322|Compound:1348-1070|FD-Score:2.78|P-value:4.29E-4 ID:SGTC_1522|Compound:st012842|FD-Score:2.73|P-value:5.30E-4 ID:SGTC_1634|Compound:st006576|FD-Score:3.77|P-value:3.12E-6 ID:SGTC_1637|Compound:st007924|FD-Score:3.70|P-value:4.55E-6 ID:SGTC_1686|Compound:st019653|FD-Score:2.85|P-value:3.15E-4 ID:SGTC_1697|Compound:st025554|FD-Score:2.75|P-value:4.92E-4 ID:SGTC_1813|Compound:st051246|FD-Score:2.90|P-value:2.56E-4 ID:SGTC_1848|Compound:st056003|FD-Score:-2.68|P-value:5.92E-4 ID:SGTC_1850|Compound:st056238|FD-Score:-2.82|P-value:3.28E-4 ID:SGTC_1874|Compound:staurosporine|FD-Score:4.52|P-value:2.88E-8 ID:SGTC_1879|Compound:gemcitabine|FD-Score:3.05|P-value:1.25E-4 ID:SGTC_1991|Compound:st073000|FD-Score:2.65|P-value:7.56E-4 ID:SGTC_2012|Compound:4023501|FD-Score:-2.83|P-value:3.12E-4 ID:SGTC_2035|Compound:5154463|FD-Score:4.45|P-value:4.54E-8 ID:SGTC_2102|Compound:5377816|FD-Score:2.94|P-value:2.16E-4 ID:SGTC_2119|Compound:5528445|FD-Score:3.19|P-value:6.52E-5 ID:SGTC_2167|Compound:5876490|FD-Score:2.68|P-value:6.72E-4 ID:SGTC_2327|Compound:6189656|FD-Score:2.68|P-value:6.56E-4 ID:SGTC_2353|Compound:9059630|FD-Score:2.87|P-value:2.93E-4 ID:SGTC_2386|Compound:9075165|FD-Score:-2.92|P-value:2.07E-4 ID:SGTC_2446|Compound:5162541|FD-Score:-2.95|P-value:1.82E-4 ID:SGTC_2453|Compound:5731132|FD-Score:3.17|P-value:7.25E-5 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:2.59|P-value:9.64E-4 ID:SGTC_2678|Compound:equilin|FD-Score:2.88|P-value:2.83E-4 ID:SGTC_2988|Compound:9073640|FD-Score:5.81|P-value:1.49E-12 ID:SGTC_3001|Compound:9076041|FD-Score:3.32|P-value:3.43E-5 ID:SGTC_3012|Compound:9080433|FD-Score:3.24|P-value:5.18E-5 ID:SGTC_3019|Compound:9081971|FD-Score:2.86|P-value:3.07E-4 ID:SGTC_3131|Compound:9127911|FD-Score:2.78|P-value:4.33E-4 ID:SGTC_3140|Compound:9094243|FD-Score:-4.61|P-value:1.34E-8 ID:SGTC_3154|Compound:9100113|FD-Score:-2.58|P-value:8.90E-4 ID:SGTC_3162|Compound:9099653|FD-Score:2.64|P-value:7.74E-4 ID:SGTC_3308|Compound:9128332|FD-Score:2.59|P-value:9.66E-4 ID:SGTC_3309|Compound:9129340|FD-Score:-2.90|P-value:2.30E-4 ID:SGTC_35|Compound:0133-0054|FD-Score:2.63|P-value:8.03E-4 ID:SGTC_48|Compound:0382-0018|FD-Score:3.03|P-value:1.39E-4 ID:SGTC_225|Compound:nsc-122657|FD-Score:-3.51|P-value:1.11E-5 ID:SGTC_260|Compound:nsc-65238|FD-Score:-3.15|P-value:6.97E-5 ID:SGTC_401|Compound:neomycin|FD-Score:-3.33|P-value:2.91E-5 ID:SGTC_535|Compound:2293-4129|FD-Score:2.59|P-value:9.51E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:2.78|P-value:4.39E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:2.92|P-value:2.29E-4 ID:SGTC_729|Compound:4092-0585|FD-Score:2.97|P-value:1.87E-4 ID:SGTC_894|Compound:0960-0238|FD-Score:3.12|P-value:9.14E-5 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:-2.57|P-value:9.58E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:-2.80|P-value:3.55E-4 ID:SGTC_1171|Compound:0368-0077|FD-Score:2.78|P-value:4.25E-4 ID:SGTC_1177|Compound:0983-0204|FD-Score:-4.41|P-value:5.20E-8 ID:SGTC_1210|Compound:3013-0140|FD-Score:3.26|P-value:4.65E-5 ID:SGTC_1306|Compound:1185-0270|FD-Score:6.34|P-value:1.36E-14 ID:SGTC_1322|Compound:1348-1070|FD-Score:2.78|P-value:4.29E-4 ID:SGTC_1522|Compound:st012842|FD-Score:2.73|P-value:5.30E-4 ID:SGTC_1634|Compound:st006576|FD-Score:3.77|P-value:3.12E-6 ID:SGTC_1637|Compound:st007924|FD-Score:3.70|P-value:4.55E-6 ID:SGTC_1686|Compound:st019653|FD-Score:2.85|P-value:3.15E-4 ID:SGTC_1697|Compound:st025554|FD-Score:2.75|P-value:4.92E-4 ID:SGTC_1813|Compound:st051246|FD-Score:2.90|P-value:2.56E-4 ID:SGTC_1848|Compound:st056003|FD-Score:-2.68|P-value:5.92E-4 ID:SGTC_1850|Compound:st056238|FD-Score:-2.82|P-value:3.28E-4 ID:SGTC_1874|Compound:staurosporine|FD-Score:4.52|P-value:2.88E-8 ID:SGTC_1879|Compound:gemcitabine|FD-Score:3.05|P-value:1.25E-4 ID:SGTC_1991|Compound:st073000|FD-Score:2.65|P-value:7.56E-4 ID:SGTC_2012|Compound:4023501|FD-Score:-2.83|P-value:3.12E-4 ID:SGTC_2035|Compound:5154463|FD-Score:4.45|P-value:4.54E-8 ID:SGTC_2102|Compound:5377816|FD-Score:2.94|P-value:2.16E-4 ID:SGTC_2119|Compound:5528445|FD-Score:3.19|P-value:6.52E-5 ID:SGTC_2167|Compound:5876490|FD-Score:2.68|P-value:6.72E-4 ID:SGTC_2327|Compound:6189656|FD-Score:2.68|P-value:6.56E-4 ID:SGTC_2353|Compound:9059630|FD-Score:2.87|P-value:2.93E-4 ID:SGTC_2386|Compound:9075165|FD-Score:-2.92|P-value:2.07E-4 ID:SGTC_2446|Compound:5162541|FD-Score:-2.95|P-value:1.82E-4 ID:SGTC_2453|Compound:5731132|FD-Score:3.17|P-value:7.25E-5 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:2.59|P-value:9.64E-4 ID:SGTC_2678|Compound:equilin|FD-Score:2.88|P-value:2.83E-4 ID:SGTC_2988|Compound:9073640|FD-Score:5.81|P-value:1.49E-12 ID:SGTC_3001|Compound:9076041|FD-Score:3.32|P-value:3.43E-5 ID:SGTC_3012|Compound:9080433|FD-Score:3.24|P-value:5.18E-5 ID:SGTC_3019|Compound:9081971|FD-Score:2.86|P-value:3.07E-4 ID:SGTC_3131|Compound:9127911|FD-Score:2.78|P-value:4.33E-4 ID:SGTC_3140|Compound:9094243|FD-Score:-4.61|P-value:1.34E-8 ID:SGTC_3154|Compound:9100113|FD-Score:-2.58|P-value:8.90E-4 ID:SGTC_3162|Compound:9099653|FD-Score:2.64|P-value:7.74E-4 ID:SGTC_3308|Compound:9128332|FD-Score:2.59|P-value:9.66E-4 ID:SGTC_3309|Compound:9129340|FD-Score:-2.90|P-value:2.30E-4

Top fitness defect scores for YPR175W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1306 1185-0270 6.34 9.0 µM 1.36E-14 Golgi 5 1.18E-10 1.22
2 SGTC_2988 9073640 5.81 71.4 µM 1.49E-12 4 3.11E-9 0.34
3 SGTC_1874 staurosporine 4.52 7.6 µM 2.88E-8 cell wall signaling 3 3.09E-6 0.60
4 SGTC_2035 5154463 4.45 94.6 µM 4.54E-8 7 4.25E-6 0.01
5 SGTC_1634 st006576 3.77 41.0 µM 3.12E-6 TSC3-RPN4 7 8.30E-5 0.00
6 SGTC_1637 st007924 3.70 17.4 µM 4.55E-6 6 1.08E-4 0.17
7 SGTC_3001 9076041 3.32 71.4 µM 3.43E-5 10 4.51E-4 0.06
8 SGTC_1210 3013-0140 3.26 750.0 nM 4.65E-5 6 5.59E-4 0.16
9 SGTC_3012 9080433 3.24 71.4 µM 5.18E-5 11 6.04E-4 0.01
10 SGTC_2119 5528445 3.19 120.6 µM 6.52E-5 9 7.10E-4 0.03
11 SGTC_2453 5731132 3.17 22.2 µM 7.25E-5 14 7.66E-4 0.04
12 SGTC_894 0960-0238 3.12 55.9 µM 9.14E-5 15 9.03E-4 0.09
13 SGTC_1879 gemcitabine 3.05 100.0 µM 1.25E-4 7 0.00113 0.10
14 SGTC_48 0382-0018 3.03 55.0 µM 1.39E-4 3 0.00122 0.04
15 SGTC_729 4092-0585 2.97 94.1 µM 1.87E-4 9 0.00150 0.00
16 SGTC_2102 5377816 2.94 72.8 µM 2.16E-4 13 0.00166 0.14
17 SGTC_633 0437-0093 2.92 97.7 µM 2.29E-4 DNA intercalators 17 0.00173 0.01
18 SGTC_1813 st051246 2.90 8.6 µM 2.56E-4 TSC3-RPN4 6 0.00187 0.01
19 SGTC_2678 equilin 2.88 100.0 µM 2.83E-4 9 0.00201 0.03
20 SGTC_2353 9059630 2.87 146.7 µM 2.93E-4 6 0.00206 0.06

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.164 1.32E-21 YLR309C IMH1 hom Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi
0.142 1.54E-16 YLL013C PUF3 hom Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins
0.140 3.17E-16 YPR176C BET2 het Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
0.138 1.15E-15 YLR190W MMR1 hom Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p
0.131 3.10E-14 YNL092W_p YNL092W_p hom Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene
0.123 8.33E-13 YJL032W_d YJL032W_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase
0.112 7.24E-11 YLR269C_d YLR269C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.112 8.83E-11 YGL091C NBP35 het Essential iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases
0.095 3.06E-8 YIR009W MSL1 hom U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members
0.094 5.18E-8 YPL051W ARL3 hom GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor
0.090 1.59E-7 YFR051C RET2 het Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
0.089 2.41E-7 YDL153C SAS10 het Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance
0.089 2.55E-7 YBR164C ARL1 hom Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor
0.088 3.24E-7 YOR216C RUD3 hom Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1
0.085 7.73E-7 YLL005C SPO75 hom Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis