YPR178W / PRP4

Splicing factor, component of the U4/U6-U5 snRNP complex

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPR178W / PRP4

Click on Significant Values for Screen Details ID:SGTC_14|Compound:0354-0009|FD-Score:3.93|P-value:6.70E-6 ID:SGTC_21|Compound:0849-0113|FD-Score:-3.44|P-value:8.94E-5 ID:SGTC_55|Compound:0767-0285|FD-Score:3.15|P-value:2.40E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:3.44|P-value:6.73E-5 ID:SGTC_98|Compound:0074-0023|FD-Score:-3.20|P-value:2.43E-4 ID:SGTC_128|Compound:0906-2981|FD-Score:-3.34|P-value:1.35E-4 ID:SGTC_142|Compound:0335-0849|FD-Score:3.10|P-value:2.86E-4 ID:SGTC_259|Compound:usnic acid|FD-Score:7.79|P-value:3.77E-18 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:5.23|P-value:3.61E-9 ID:SGTC_276|Compound:colchicine|FD-Score:3.28|P-value:1.37E-4 ID:SGTC_419|Compound:nsc-638432|FD-Score:3.23|P-value:1.66E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:3.70|P-value:2.09E-5 ID:SGTC_554|Compound:4483-2211|FD-Score:2.86|P-value:7.43E-4 ID:SGTC_641|Compound:4092-0595|FD-Score:4.10|P-value:2.85E-6 ID:SGTC_666|Compound:0272-0154|FD-Score:2.98|P-value:4.65E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:3.51|P-value:4.91E-5 ID:SGTC_1011|Compound:4112-3381|FD-Score:2.92|P-value:5.83E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:3.02|P-value:3.93E-4 ID:SGTC_1052|Compound:3253-3998|FD-Score:-4.02|P-value:5.65E-6 ID:SGTC_1059|Compound:3448-6867|FD-Score:3.86|P-value:9.24E-6 ID:SGTC_1167|Compound:k781-0610|FD-Score:3.23|P-value:1.66E-4 ID:SGTC_1169|Compound:aniline yellow|FD-Score:4.19|P-value:1.76E-6 ID:SGTC_1256|Compound:0570-0116|FD-Score:3.30|P-value:1.24E-4 ID:SGTC_1291|Compound:0993-0025|FD-Score:3.14|P-value:2.50E-4 ID:SGTC_1314|Compound:1222-0010|FD-Score:2.82|P-value:8.48E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:2.82|P-value:8.57E-4 ID:SGTC_1375|Compound:2910-0797|FD-Score:3.86|P-value:9.47E-6 ID:SGTC_1454|Compound:4239-0054|FD-Score:-3.31|P-value:1.57E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:2.88|P-value:6.98E-4 ID:SGTC_1720|Compound:st037298|FD-Score:3.11|P-value:2.80E-4 ID:SGTC_1801|Compound:st050089|FD-Score:3.69|P-value:2.11E-5 ID:SGTC_1837|Compound:st055472|FD-Score:3.88|P-value:8.67E-6 ID:SGTC_1840|Compound:st055363|FD-Score:-2.90|P-value:8.07E-4 ID:SGTC_1930|Compound:st074634|FD-Score:3.01|P-value:4.13E-4 ID:SGTC_2023|Compound:5144124|FD-Score:-4.23|P-value:1.95E-6 ID:SGTC_2118|Compound:5526350|FD-Score:3.05|P-value:3.56E-4 ID:SGTC_2127|Compound:5253358|FD-Score:2.82|P-value:8.69E-4 ID:SGTC_2155|Compound:5570423|FD-Score:3.31|P-value:1.20E-4 ID:SGTC_2273|Compound:7815444|FD-Score:-2.85|P-value:9.71E-4 ID:SGTC_2283|Compound:7944793|FD-Score:3.06|P-value:3.34E-4 ID:SGTC_2305|Compound:7443522|FD-Score:-2.87|P-value:8.93E-4 ID:SGTC_2438|Compound:5259590|FD-Score:-2.87|P-value:9.03E-4 ID:SGTC_2443|Compound:5493264|FD-Score:4.10|P-value:2.79E-6 ID:SGTC_2444|Compound:5485484|FD-Score:3.00|P-value:4.27E-4 ID:SGTC_2493|Compound:resveratrol|FD-Score:5.12|P-value:7.54E-9 ID:SGTC_2667|Compound:digitoxin|FD-Score:3.75|P-value:1.60E-5 ID:SGTC_2696|Compound:st077691|FD-Score:-3.12|P-value:3.37E-4 ID:SGTC_3028|Compound:9087778|FD-Score:-3.24|P-value:2.07E-4 ID:SGTC_3105|Compound:9121040|FD-Score:3.30|P-value:1.28E-4 ID:SGTC_3193|Compound:9111931|FD-Score:-3.49|P-value:7.15E-5 ID:SGTC_3196|Compound:9111891|FD-Score:-4.14|P-value:3.01E-6 ID:SGTC_3198|Compound:9112019|FD-Score:-3.35|P-value:1.32E-4 ID:SGTC_3225|Compound:9130819|FD-Score:-3.29|P-value:1.69E-4 ID:SGTC_3284|Compound:9108171|FD-Score:4.23|P-value:1.43E-6 ID:SGTC_3287|Compound:9117173|FD-Score:6.17|P-value:4.51E-12 ID:SGTC_3292|Compound:9120825|FD-Score:3.45|P-value:6.56E-5 ID:SGTC_3297|Compound:9117502|FD-Score:3.35|P-value:1.02E-4 ID:SGTC_14|Compound:0354-0009|FD-Score:3.93|P-value:6.70E-6 ID:SGTC_21|Compound:0849-0113|FD-Score:-3.44|P-value:8.94E-5 ID:SGTC_55|Compound:0767-0285|FD-Score:3.15|P-value:2.40E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:3.44|P-value:6.73E-5 ID:SGTC_98|Compound:0074-0023|FD-Score:-3.20|P-value:2.43E-4 ID:SGTC_128|Compound:0906-2981|FD-Score:-3.34|P-value:1.35E-4 ID:SGTC_142|Compound:0335-0849|FD-Score:3.10|P-value:2.86E-4 ID:SGTC_259|Compound:usnic acid|FD-Score:7.79|P-value:3.77E-18 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:5.23|P-value:3.61E-9 ID:SGTC_276|Compound:colchicine|FD-Score:3.28|P-value:1.37E-4 ID:SGTC_419|Compound:nsc-638432|FD-Score:3.23|P-value:1.66E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:3.70|P-value:2.09E-5 ID:SGTC_554|Compound:4483-2211|FD-Score:2.86|P-value:7.43E-4 ID:SGTC_641|Compound:4092-0595|FD-Score:4.10|P-value:2.85E-6 ID:SGTC_666|Compound:0272-0154|FD-Score:2.98|P-value:4.65E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:3.51|P-value:4.91E-5 ID:SGTC_1011|Compound:4112-3381|FD-Score:2.92|P-value:5.83E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:3.02|P-value:3.93E-4 ID:SGTC_1052|Compound:3253-3998|FD-Score:-4.02|P-value:5.65E-6 ID:SGTC_1059|Compound:3448-6867|FD-Score:3.86|P-value:9.24E-6 ID:SGTC_1167|Compound:k781-0610|FD-Score:3.23|P-value:1.66E-4 ID:SGTC_1169|Compound:aniline yellow|FD-Score:4.19|P-value:1.76E-6 ID:SGTC_1256|Compound:0570-0116|FD-Score:3.30|P-value:1.24E-4 ID:SGTC_1291|Compound:0993-0025|FD-Score:3.14|P-value:2.50E-4 ID:SGTC_1314|Compound:1222-0010|FD-Score:2.82|P-value:8.48E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:2.82|P-value:8.57E-4 ID:SGTC_1375|Compound:2910-0797|FD-Score:3.86|P-value:9.47E-6 ID:SGTC_1454|Compound:4239-0054|FD-Score:-3.31|P-value:1.57E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:2.88|P-value:6.98E-4 ID:SGTC_1720|Compound:st037298|FD-Score:3.11|P-value:2.80E-4 ID:SGTC_1801|Compound:st050089|FD-Score:3.69|P-value:2.11E-5 ID:SGTC_1837|Compound:st055472|FD-Score:3.88|P-value:8.67E-6 ID:SGTC_1840|Compound:st055363|FD-Score:-2.90|P-value:8.07E-4 ID:SGTC_1930|Compound:st074634|FD-Score:3.01|P-value:4.13E-4 ID:SGTC_2023|Compound:5144124|FD-Score:-4.23|P-value:1.95E-6 ID:SGTC_2118|Compound:5526350|FD-Score:3.05|P-value:3.56E-4 ID:SGTC_2127|Compound:5253358|FD-Score:2.82|P-value:8.69E-4 ID:SGTC_2155|Compound:5570423|FD-Score:3.31|P-value:1.20E-4 ID:SGTC_2273|Compound:7815444|FD-Score:-2.85|P-value:9.71E-4 ID:SGTC_2283|Compound:7944793|FD-Score:3.06|P-value:3.34E-4 ID:SGTC_2305|Compound:7443522|FD-Score:-2.87|P-value:8.93E-4 ID:SGTC_2438|Compound:5259590|FD-Score:-2.87|P-value:9.03E-4 ID:SGTC_2443|Compound:5493264|FD-Score:4.10|P-value:2.79E-6 ID:SGTC_2444|Compound:5485484|FD-Score:3.00|P-value:4.27E-4 ID:SGTC_2493|Compound:resveratrol|FD-Score:5.12|P-value:7.54E-9 ID:SGTC_2667|Compound:digitoxin|FD-Score:3.75|P-value:1.60E-5 ID:SGTC_2696|Compound:st077691|FD-Score:-3.12|P-value:3.37E-4 ID:SGTC_3028|Compound:9087778|FD-Score:-3.24|P-value:2.07E-4 ID:SGTC_3105|Compound:9121040|FD-Score:3.30|P-value:1.28E-4 ID:SGTC_3193|Compound:9111931|FD-Score:-3.49|P-value:7.15E-5 ID:SGTC_3196|Compound:9111891|FD-Score:-4.14|P-value:3.01E-6 ID:SGTC_3198|Compound:9112019|FD-Score:-3.35|P-value:1.32E-4 ID:SGTC_3225|Compound:9130819|FD-Score:-3.29|P-value:1.69E-4 ID:SGTC_3284|Compound:9108171|FD-Score:4.23|P-value:1.43E-6 ID:SGTC_3287|Compound:9117173|FD-Score:6.17|P-value:4.51E-12 ID:SGTC_3292|Compound:9120825|FD-Score:3.45|P-value:6.56E-5 ID:SGTC_3297|Compound:9117502|FD-Score:3.35|P-value:1.02E-4

Top fitness defect scores for YPR178W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_259 usnic acid 7.79 108.9 µM 3.77E-18 1 3.23E-15 2.09
2 SGTC_3287 9117173 6.17 37.1 µM 4.51E-12 60S ribosome export 1 3.37E-10 2.25
3 SGTC_265 mycophenolic acid 5.23 32.9 µM 3.61E-9 RPP1 & pyrimidine depletion 2 8.47E-8 0.64
4 SGTC_2493 resveratrol 5.12 26.1 µM 7.54E-9 1 1.56E-7 1.05
5 SGTC_3284 9108171 4.23 64.9 µM 1.43E-6 4 1.19E-5 0.68
6 SGTC_1169 aniline yellow 4.19 25.8 µM 1.76E-6 1 1.42E-5 0.68
7 SGTC_2443 5493264 4.10 126.7 µM 2.79E-6 4 2.07E-5 0.04
8 SGTC_641 4092-0595 4.10 30.0 µM 2.85E-6 9 2.11E-5 0.26
9 SGTC_14 0354-0009 3.93 295.0 µM 6.70E-6 3 4.28E-5 0.30
10 SGTC_1837 st055472 3.88 66.5 µM 8.67E-6 3 5.30E-5 0.48
11 SGTC_1059 3448-6867 3.86 171.0 µM 9.24E-6 5 5.58E-5 0.04
12 SGTC_1375 2910-0797 3.86 186.0 µM 9.47E-6 6 5.70E-5 0.25
13 SGTC_2667 digitoxin 3.75 100.0 µM 1.60E-5 1 8.82E-5 0.90
14 SGTC_519 k048-0011 3.70 62.8 µM 2.09E-5 10 1.10E-4 0.19
15 SGTC_1801 st050089 3.69 28.4 µM 2.11E-5 NEO1-PIK1 12 1.10E-4 0.04
16 SGTC_943 3253-1379 3.51 105.0 µM 4.91E-5 11 2.23E-4 0.04
17 SGTC_3292 9120825 3.45 59.8 µM 6.56E-5 5 2.83E-4 0.06
18 SGTC_64 000l-0018 3.44 272.1 µM 6.73E-5 ubiquinone biosynthesis & proteasome 58 2.90E-4 8.79E-4
19 SGTC_3297 9117502 3.35 71.4 µM 1.02E-4 1 4.08E-4 0.61
20 SGTC_2155 5570423 3.31 76.0 µM 1.20E-4 ergosterol biosynthesis 9 4.68E-4 0.07

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.148 6.50E-18 YLR290C_p YLR290C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene
0.118 5.99E-12 YDR122W KIN1 hom Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p
0.107 5.59E-10 YGL044C RNA15 het Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
0.105 1.19E-9 YPL141C FRK1 hom Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication
0.098 1.48E-8 YLR276C DBP9 het DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain
0.094 5.41E-8 YPR146C_d YPR146C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.094 5.52E-8 YBL015W ACH1 hom Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth
0.091 1.20E-7 YBR052C RFS1 hom Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication
0.090 1.97E-7 YGL119W COQ8 hom Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.089 2.59E-7 YNL111C CYB5 hom Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
0.087 4.96E-7 YFR024C-A LSB3 hom Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication
0.086 6.01E-7 YDR355C_d YDR355C_d het Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W
0.084 1.06E-6 YDL183C YDL183C hom Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene
0.083 1.64E-6 YNL217W_p YNL217W_p hom Putative protein of unknown function; weak sequence similarity to bis (5'-nucleotidyl)-tetraphosphatases; (GFP)-fusion protein localizes to the vacuole; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate
0.082 1.93E-6 YER109C FLO8 hom Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene