YPR180W / AOS1

Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPR180W / AOS1

Click on Significant Values for Screen Details ID:SGTC_213|Compound:1246-0773|FD-Score:3.45|P-value:2.60E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:-3.12|P-value:8.30E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:3.67|P-value:1.08E-4 ID:SGTC_370|Compound:0180-0345|FD-Score:-3.39|P-value:3.12E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:-3.61|P-value:1.36E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:3.16|P-value:7.18E-4 ID:SGTC_667|Compound:1498-1044|FD-Score:3.31|P-value:4.28E-4 ID:SGTC_716|Compound:1683-5115|FD-Score:-3.18|P-value:6.61E-4 ID:SGTC_1015|Compound:4130-4525|FD-Score:3.12|P-value:8.35E-4 ID:SGTC_1212|Compound:0520-0165|FD-Score:-5.52|P-value:1.33E-8 ID:SGTC_1213|Compound:1315-0166|FD-Score:-4.55|P-value:2.22E-6 ID:SGTC_1214|Compound:0358-0031|FD-Score:-11.90|P-value:1.14E-33 ID:SGTC_1240|Compound:0356-0016|FD-Score:-7.23|P-value:1.59E-13 ID:SGTC_1451|Compound:4152-0210|FD-Score:3.63|P-value:1.27E-4 ID:SGTC_1460|Compound:k062-0059|FD-Score:-4.40|P-value:4.63E-6 ID:SGTC_1461|Compound:k072-0203|FD-Score:-8.08|P-value:1.95E-16 ID:SGTC_1462|Compound:k081-0014|FD-Score:-15.00|P-value:3.92E-52 ID:SGTC_1463|Compound:k081-0032|FD-Score:-15.50|P-value:2.29E-55 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:3.86|P-value:5.00E-5 ID:SGTC_1637|Compound:st007924|FD-Score:-3.26|P-value:5.09E-4 ID:SGTC_1638|Compound:st008370|FD-Score:-3.21|P-value:6.08E-4 ID:SGTC_1730|Compound:st033808|FD-Score:-3.51|P-value:2.00E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-3.40|P-value:3.09E-4 ID:SGTC_2018|Compound:5133068|FD-Score:-3.16|P-value:7.11E-4 ID:SGTC_2023|Compound:5144124|FD-Score:-3.07|P-value:9.67E-4 ID:SGTC_2341|Compound:7987486|FD-Score:4.05|P-value:2.23E-5 ID:SGTC_2461|Compound:5755302|FD-Score:3.37|P-value:3.40E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:-3.22|P-value:5.92E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:4.31|P-value:7.05E-6 ID:SGTC_2575|Compound:plumbagin|FD-Score:-4.65|P-value:1.41E-6 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:-3.73|P-value:8.47E-5 ID:SGTC_2583|Compound:asiatic acid|FD-Score:3.63|P-value:1.29E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:4.13|P-value:1.57E-5 ID:SGTC_2646|Compound:retinol|FD-Score:3.36|P-value:3.60E-4 ID:SGTC_3021|Compound:9087658|FD-Score:4.13|P-value:1.58E-5 ID:SGTC_3025|Compound:9087895|FD-Score:3.61|P-value:1.36E-4 ID:SGTC_3206|Compound:9114350|FD-Score:-3.21|P-value:6.08E-4 ID:SGTC_3251|Compound:9135047|FD-Score:3.60|P-value:1.42E-4 ID:SGTC_213|Compound:1246-0773|FD-Score:3.45|P-value:2.60E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:-3.12|P-value:8.30E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:3.67|P-value:1.08E-4 ID:SGTC_370|Compound:0180-0345|FD-Score:-3.39|P-value:3.12E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:-3.61|P-value:1.36E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:3.16|P-value:7.18E-4 ID:SGTC_667|Compound:1498-1044|FD-Score:3.31|P-value:4.28E-4 ID:SGTC_716|Compound:1683-5115|FD-Score:-3.18|P-value:6.61E-4 ID:SGTC_1015|Compound:4130-4525|FD-Score:3.12|P-value:8.35E-4 ID:SGTC_1212|Compound:0520-0165|FD-Score:-5.52|P-value:1.33E-8 ID:SGTC_1213|Compound:1315-0166|FD-Score:-4.55|P-value:2.22E-6 ID:SGTC_1214|Compound:0358-0031|FD-Score:-11.90|P-value:1.14E-33 ID:SGTC_1240|Compound:0356-0016|FD-Score:-7.23|P-value:1.59E-13 ID:SGTC_1451|Compound:4152-0210|FD-Score:3.63|P-value:1.27E-4 ID:SGTC_1460|Compound:k062-0059|FD-Score:-4.40|P-value:4.63E-6 ID:SGTC_1461|Compound:k072-0203|FD-Score:-8.08|P-value:1.95E-16 ID:SGTC_1462|Compound:k081-0014|FD-Score:-15.00|P-value:3.92E-52 ID:SGTC_1463|Compound:k081-0032|FD-Score:-15.50|P-value:2.29E-55 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:3.86|P-value:5.00E-5 ID:SGTC_1637|Compound:st007924|FD-Score:-3.26|P-value:5.09E-4 ID:SGTC_1638|Compound:st008370|FD-Score:-3.21|P-value:6.08E-4 ID:SGTC_1730|Compound:st033808|FD-Score:-3.51|P-value:2.00E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-3.40|P-value:3.09E-4 ID:SGTC_2018|Compound:5133068|FD-Score:-3.16|P-value:7.11E-4 ID:SGTC_2023|Compound:5144124|FD-Score:-3.07|P-value:9.67E-4 ID:SGTC_2341|Compound:7987486|FD-Score:4.05|P-value:2.23E-5 ID:SGTC_2461|Compound:5755302|FD-Score:3.37|P-value:3.40E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:-3.22|P-value:5.92E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:4.31|P-value:7.05E-6 ID:SGTC_2575|Compound:plumbagin|FD-Score:-4.65|P-value:1.41E-6 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:-3.73|P-value:8.47E-5 ID:SGTC_2583|Compound:asiatic acid|FD-Score:3.63|P-value:1.29E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:4.13|P-value:1.57E-5 ID:SGTC_2646|Compound:retinol|FD-Score:3.36|P-value:3.60E-4 ID:SGTC_3021|Compound:9087658|FD-Score:4.13|P-value:1.58E-5 ID:SGTC_3025|Compound:9087895|FD-Score:3.61|P-value:1.36E-4 ID:SGTC_3206|Compound:9114350|FD-Score:-3.21|P-value:6.08E-4 ID:SGTC_3251|Compound:9135047|FD-Score:3.60|P-value:1.42E-4

Top fitness defect scores for YPR180W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2502 avocadyne acetate 4.31 18.6 µM 7.05E-6 6 8.14E-6 0.72
2 SGTC_2634 4'-methoxyflavone 4.13 100.0 µM 1.57E-5 6 1.79E-5 0.03
3 SGTC_3021 9087658 4.13 49.5 µM 1.58E-5 3 1.80E-5 0.53
4 SGTC_2341 7987486 4.05 176.7 µM 2.23E-5 6 2.53E-5 0.33
5 SGTC_1587 estradiol valerate 3.86 56.1 µM 5.00E-5 3 5.61E-5 0.16
6 SGTC_358 0075-0058 3.67 9.4 µM 1.08E-4 3 1.20E-4 0.00
7 SGTC_1451 4152-0210 3.63 104.0 µM 1.27E-4 6 1.40E-4 0.20
8 SGTC_2583 asiatic acid 3.63 100.0 µM 1.29E-4 13 1.43E-4 0.32
9 SGTC_3025 9087895 3.61 49.5 µM 1.36E-4 PDR1 6 1.50E-4 0.03
10 SGTC_3251 9135047 3.60 49.5 µM 1.42E-4 60S ribosome export 7 1.57E-4 0.33
11 SGTC_213 1246-0773 3.45 967.0 nM 2.60E-4 5 2.85E-4 0.19
12 SGTC_2461 5755302 3.37 26.1 µM 3.40E-4 8 3.71E-4 0.06
13 SGTC_2646 retinol 3.36 96.8 µM 3.60E-4 7 3.93E-4 0.04
14 SGTC_667 1498-1044 3.31 59.0 µM 4.28E-4 RPP1 & pyrimidine depletion 15 4.66E-4 0.04
15 SGTC_573 3702-0553 3.16 84.7 µM 7.18E-4 12 7.76E-4 0.18
16 SGTC_1015 4130-4525 3.12 82.0 µM 8.35E-4 60S ribosome export 17 9.01E-4 0.01
17 SGTC_2831 7999036 3.04 19.5 µM 0.00112 14 0.00120 0.00
18 SGTC_531 3788-1495 3.03 174.0 µM 0.00113 unfolded protein response 13 0.00121 0.00
19 SGTC_1695 st026914 3.03 34.5 µM 0.00114 12 0.00122 0.03
20 SGTC_3191 9110352 3.03 49.5 µM 0.00115 5 0.00123 0.06

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.511 4.20E-221 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.486 1.91E-197 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.453 7.51E-168 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.433 1.28E-152 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.399 1.47E-124 YKR099W BAS1 hom Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes
0.397 4.68E-126 YDL176W YDL176W hom Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.367 3.61E-107 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.362 3.83E-104 YDL157C_p YDL157C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.354 9.83E-99 YDR312W SSF2 hom Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication
0.330 2.06E-85 YFL014W HSP12 hom Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
0.329 4.63E-85 YDR169C STB3 hom Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress
0.326 2.42E-83 YGR136W LSB1 hom Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress
0.324 2.75E-82 YMR137C PSO2 hom Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress
0.323 1.55E-81 YCL026C-A FRM2 hom Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin
0.322 5.39E-81 YDL222C FMP45 hom Integral membrane protein localized to mitochondria (untagged protein); required for sporulation and maintaining sphingolipid content; has sequence similarity to SUR7 and YNL194C