YPR186C / PZF1

Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPR186C / PZF1

Click on Significant Values for Screen Details ID:SGTC_74|Compound:000s-0487|FD-Score:-5.20|P-value:1.59E-8 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:3.03|P-value:7.05E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:3.70|P-value:4.56E-5 ID:SGTC_323|Compound:4013-0906|FD-Score:3.65|P-value:5.77E-5 ID:SGTC_517|Compound:1365-0151|FD-Score:3.03|P-value:6.85E-4 ID:SGTC_537|Compound:1334-0398|FD-Score:3.56|P-value:8.43E-5 ID:SGTC_540|Compound:1187-0821|FD-Score:2.99|P-value:7.98E-4 ID:SGTC_564|Compound:3555-0129|FD-Score:3.94|P-value:1.60E-5 ID:SGTC_628|Compound:1636-0253|FD-Score:3.29|P-value:2.59E-4 ID:SGTC_659|Compound:k018-0002|FD-Score:3.00|P-value:7.76E-4 ID:SGTC_802|Compound:4112-3352|FD-Score:-3.21|P-value:3.18E-4 ID:SGTC_1062|Compound:mesoridazine|FD-Score:-2.98|P-value:7.50E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.08|P-value:5.81E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:4.68|P-value:3.70E-7 ID:SGTC_1244|Compound:0416-0015|FD-Score:-2.92|P-value:9.29E-4 ID:SGTC_1259|Compound:0671-0132|FD-Score:3.08|P-value:5.78E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:4.30|P-value:2.75E-6 ID:SGTC_1468|Compound:k284-1086|FD-Score:3.47|P-value:1.23E-4 ID:SGTC_1470|Compound:k295-0292|FD-Score:4.00|P-value:1.20E-5 ID:SGTC_1494|Compound:4358-1428|FD-Score:3.62|P-value:6.59E-5 ID:SGTC_1502|Compound:4534-4282|FD-Score:-3.53|P-value:8.54E-5 ID:SGTC_1713|Compound:st033146|FD-Score:4.74|P-value:2.64E-7 ID:SGTC_1714|Compound:st033236|FD-Score:3.65|P-value:5.86E-5 ID:SGTC_1717|Compound:st033143|FD-Score:3.75|P-value:3.75E-5 ID:SGTC_1738|Compound:st035555|FD-Score:-3.09|P-value:5.02E-4 ID:SGTC_1758|Compound:st042942|FD-Score:2.95|P-value:9.16E-4 ID:SGTC_1879|Compound:gemcitabine|FD-Score:2.95|P-value:9.19E-4 ID:SGTC_1880|Compound:MG-132|FD-Score:3.09|P-value:5.53E-4 ID:SGTC_1929|Compound:genistein trimethyl ether|FD-Score:3.27|P-value:2.75E-4 ID:SGTC_2015|Compound:5119600|FD-Score:4.17|P-value:5.23E-6 ID:SGTC_2024|Compound:5144227|FD-Score:3.19|P-value:3.80E-4 ID:SGTC_2029|Compound:5175081|FD-Score:-3.08|P-value:5.10E-4 ID:SGTC_2048|Compound:5246248|FD-Score:-3.67|P-value:4.64E-5 ID:SGTC_2054|Compound:5233173|FD-Score:-3.59|P-value:6.52E-5 ID:SGTC_2086|Compound:5274770|FD-Score:2.95|P-value:9.42E-4 ID:SGTC_2139|Compound:5578692|FD-Score:3.57|P-value:8.16E-5 ID:SGTC_2143|Compound:5623677|FD-Score:4.31|P-value:2.60E-6 ID:SGTC_2147|Compound:5545932|FD-Score:3.34|P-value:2.08E-4 ID:SGTC_2158|Compound:5657123|FD-Score:3.10|P-value:5.26E-4 ID:SGTC_2428|Compound:5615643|FD-Score:5.20|P-value:1.90E-8 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:-2.90|P-value:9.84E-4 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:3.99|P-value:1.27E-5 ID:SGTC_2602|Compound:kawain|FD-Score:5.72|P-value:7.14E-10 ID:SGTC_2606|Compound:farnesol|FD-Score:-3.26|P-value:2.63E-4 ID:SGTC_2706|Compound:st078239|FD-Score:-3.33|P-value:1.97E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.08|P-value:5.79E-4 ID:SGTC_2811|Compound:7967822|FD-Score:3.11|P-value:5.20E-4 ID:SGTC_2955|Compound:9076254|FD-Score:3.21|P-value:3.55E-4 ID:SGTC_2975|Compound:9084847|FD-Score:4.39|P-value:1.75E-6 ID:SGTC_2978|Compound:9011784|FD-Score:-3.46|P-value:1.14E-4 ID:SGTC_2989|Compound:9073649|FD-Score:3.29|P-value:2.58E-4 ID:SGTC_3058|Compound:9093861|FD-Score:-3.21|P-value:3.14E-4 ID:SGTC_3106|Compound:9121286|FD-Score:3.44|P-value:1.43E-4 ID:SGTC_3200|Compound:9114385|FD-Score:3.80|P-value:2.97E-5 ID:SGTC_74|Compound:000s-0487|FD-Score:-5.20|P-value:1.59E-8 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:3.03|P-value:7.05E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:3.70|P-value:4.56E-5 ID:SGTC_323|Compound:4013-0906|FD-Score:3.65|P-value:5.77E-5 ID:SGTC_517|Compound:1365-0151|FD-Score:3.03|P-value:6.85E-4 ID:SGTC_537|Compound:1334-0398|FD-Score:3.56|P-value:8.43E-5 ID:SGTC_540|Compound:1187-0821|FD-Score:2.99|P-value:7.98E-4 ID:SGTC_564|Compound:3555-0129|FD-Score:3.94|P-value:1.60E-5 ID:SGTC_628|Compound:1636-0253|FD-Score:3.29|P-value:2.59E-4 ID:SGTC_659|Compound:k018-0002|FD-Score:3.00|P-value:7.76E-4 ID:SGTC_802|Compound:4112-3352|FD-Score:-3.21|P-value:3.18E-4 ID:SGTC_1062|Compound:mesoridazine|FD-Score:-2.98|P-value:7.50E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.08|P-value:5.81E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:4.68|P-value:3.70E-7 ID:SGTC_1244|Compound:0416-0015|FD-Score:-2.92|P-value:9.29E-4 ID:SGTC_1259|Compound:0671-0132|FD-Score:3.08|P-value:5.78E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:4.30|P-value:2.75E-6 ID:SGTC_1468|Compound:k284-1086|FD-Score:3.47|P-value:1.23E-4 ID:SGTC_1470|Compound:k295-0292|FD-Score:4.00|P-value:1.20E-5 ID:SGTC_1494|Compound:4358-1428|FD-Score:3.62|P-value:6.59E-5 ID:SGTC_1502|Compound:4534-4282|FD-Score:-3.53|P-value:8.54E-5 ID:SGTC_1713|Compound:st033146|FD-Score:4.74|P-value:2.64E-7 ID:SGTC_1714|Compound:st033236|FD-Score:3.65|P-value:5.86E-5 ID:SGTC_1717|Compound:st033143|FD-Score:3.75|P-value:3.75E-5 ID:SGTC_1738|Compound:st035555|FD-Score:-3.09|P-value:5.02E-4 ID:SGTC_1758|Compound:st042942|FD-Score:2.95|P-value:9.16E-4 ID:SGTC_1879|Compound:gemcitabine|FD-Score:2.95|P-value:9.19E-4 ID:SGTC_1880|Compound:MG-132|FD-Score:3.09|P-value:5.53E-4 ID:SGTC_1929|Compound:genistein trimethyl ether|FD-Score:3.27|P-value:2.75E-4 ID:SGTC_2015|Compound:5119600|FD-Score:4.17|P-value:5.23E-6 ID:SGTC_2024|Compound:5144227|FD-Score:3.19|P-value:3.80E-4 ID:SGTC_2029|Compound:5175081|FD-Score:-3.08|P-value:5.10E-4 ID:SGTC_2048|Compound:5246248|FD-Score:-3.67|P-value:4.64E-5 ID:SGTC_2054|Compound:5233173|FD-Score:-3.59|P-value:6.52E-5 ID:SGTC_2086|Compound:5274770|FD-Score:2.95|P-value:9.42E-4 ID:SGTC_2139|Compound:5578692|FD-Score:3.57|P-value:8.16E-5 ID:SGTC_2143|Compound:5623677|FD-Score:4.31|P-value:2.60E-6 ID:SGTC_2147|Compound:5545932|FD-Score:3.34|P-value:2.08E-4 ID:SGTC_2158|Compound:5657123|FD-Score:3.10|P-value:5.26E-4 ID:SGTC_2428|Compound:5615643|FD-Score:5.20|P-value:1.90E-8 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:-2.90|P-value:9.84E-4 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:3.99|P-value:1.27E-5 ID:SGTC_2602|Compound:kawain|FD-Score:5.72|P-value:7.14E-10 ID:SGTC_2606|Compound:farnesol|FD-Score:-3.26|P-value:2.63E-4 ID:SGTC_2706|Compound:st078239|FD-Score:-3.33|P-value:1.97E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.08|P-value:5.79E-4 ID:SGTC_2811|Compound:7967822|FD-Score:3.11|P-value:5.20E-4 ID:SGTC_2955|Compound:9076254|FD-Score:3.21|P-value:3.55E-4 ID:SGTC_2975|Compound:9084847|FD-Score:4.39|P-value:1.75E-6 ID:SGTC_2978|Compound:9011784|FD-Score:-3.46|P-value:1.14E-4 ID:SGTC_2989|Compound:9073649|FD-Score:3.29|P-value:2.58E-4 ID:SGTC_3058|Compound:9093861|FD-Score:-3.21|P-value:3.14E-4 ID:SGTC_3106|Compound:9121286|FD-Score:3.44|P-value:1.43E-4 ID:SGTC_3200|Compound:9114385|FD-Score:3.80|P-value:2.97E-5

Top fitness defect scores for YPR186C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2602 kawain 5.72 100.0 µM 7.14E-10 2 5.26E-9 0.83
2 SGTC_2428 5615643 5.20 7.8 µM 1.90E-8 4 9.89E-8 0.40
3 SGTC_1713 st033146 4.74 12.8 µM 2.64E-7 5 1.04E-6 0.37
4 SGTC_1129 3453-1384 4.68 223.0 µM 3.70E-7 11 1.41E-6 0.21
5 SGTC_2975 9084847 4.39 53.7 µM 1.75E-6 4 5.69E-6 0.09
6 SGTC_2143 5623677 4.31 140.5 µM 2.60E-6 1 8.09E-6 0.63
7 SGTC_1369 1866-0067 4.30 208.0 µM 2.75E-6 RPP1 & pyrimidine depletion 8 8.52E-6 0.28
8 SGTC_2015 5119600 4.17 184.0 µM 5.23E-6 7 1.52E-5 0.30
9 SGTC_1470 k295-0292 4.00 231.0 µM 1.20E-5 3 3.19E-5 0.02
10 SGTC_2539 epi(13)torulosol 3.99 80.0 µM 1.27E-5 4 3.37E-5 0.44
11 SGTC_564 3555-0129 3.94 330.0 µM 1.60E-5 2 4.15E-5 0.07
12 SGTC_3200 9114385 3.80 49.5 µM 2.97E-5 23 7.19E-5 0.16
13 SGTC_1717 st033143 3.75 58.0 µM 3.75E-5 6 8.88E-5 0.10
14 SGTC_164 4100-3994 3.70 89.6 µM 4.56E-5 8 1.06E-4 0.03
15 SGTC_323 4013-0906 3.65 34.3 µM 5.77E-5 ERG2 3 1.31E-4 0.22
16 SGTC_1714 st033236 3.65 13.6 µM 5.86E-5 10 1.33E-4 0.00
17 SGTC_1494 4358-1428 3.62 177.0 µM 6.59E-5 7 1.47E-4 0.12
18 SGTC_2139 5578692 3.57 56.5 µM 8.16E-5 superoxide 21 1.79E-4 0.06
19 SGTC_537 1334-0398 3.56 209.0 µM 8.43E-5 12 1.84E-4 0.02
20 SGTC_1468 k284-1086 3.47 134.0 µM 1.23E-4 DNA intercalators 16 2.57E-4 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.153 5.06E-19 YLR401C DUS3 hom Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress
0.120 3.53E-12 YPR025C CCL1 het Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters
0.109 2.49E-10 YOL164W-A_p YOL164W-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.104 1.30E-9 YGR074W SMD1 het Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress
0.101 4.87E-9 YDL129W YDL129W hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress
0.100 5.68E-9 YDL233W_p MFG1_p hom Regulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene
0.098 1.20E-8 YOR376W_d YOR376W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YOR376W is not an essential gene.
0.093 5.76E-8 YER011W TIR1 hom Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking
0.090 1.56E-7 YGR161W-C_p YGR161W-C_p hom Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species
0.090 1.70E-7 YLR277C YSH1 het Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication
0.090 1.99E-7 YNL109W_d YNL109W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YNL108C
0.087 4.62E-7 YOL086W-A MHF1 hom Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1
0.086 5.30E-7 YGR207C CIR1 hom Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response
0.086 5.92E-7 YBR050C REG2 hom Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease
0.085 7.27E-7 YCR054C CTR86 het Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress