YPR189W / SKI3

Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay

Zygosity: Homozygous strain
fixedexpanded
Profile for YPR189W / SKI3

Click on Significant Values for Screen Details ID:SGTC_3|Compound:0833-0587|FD-Score:3.01|P-value:6.91E-4 ID:SGTC_49|Compound:0717-0965|FD-Score:3.17|P-value:3.85E-4 ID:SGTC_105|Compound:0111-0055|FD-Score:3.12|P-value:4.63E-4 ID:SGTC_183|Compound:3914-0051|FD-Score:3.10|P-value:4.99E-4 ID:SGTC_246|Compound:cycloheximide|FD-Score:3.93|P-value:1.58E-5 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:3.51|P-value:9.89E-5 ID:SGTC_395|Compound:anisomycin|FD-Score:2.96|P-value:8.32E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:2.94|P-value:9.01E-4 ID:SGTC_744|Compound:1594-0068|FD-Score:4.78|P-value:2.25E-7 ID:SGTC_772|Compound:k072-0172|FD-Score:-3.14|P-value:5.72E-4 ID:SGTC_789|Compound:1309-1135|FD-Score:3.81|P-value:2.78E-5 ID:SGTC_876|Compound:0720-0963|FD-Score:3.25|P-value:2.86E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:3.19|P-value:3.57E-4 ID:SGTC_935|Compound:2996-0153|FD-Score:3.27|P-value:2.66E-4 ID:SGTC_937|Compound:3122-0005|FD-Score:3.03|P-value:6.51E-4 ID:SGTC_1015|Compound:4130-4525|FD-Score:3.89|P-value:1.96E-5 ID:SGTC_1057|Compound:3448-5381|FD-Score:-3.22|P-value:4.25E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.05|P-value:7.95E-4 ID:SGTC_1273|Compound:0849-0096|FD-Score:3.42|P-value:1.43E-4 ID:SGTC_1393|Compound:3470-5649|FD-Score:5.39|P-value:6.37E-9 ID:SGTC_1399|Compound:3562-5772|FD-Score:3.63|P-value:6.01E-5 ID:SGTC_1429|Compound:4049-0205|FD-Score:3.08|P-value:5.46E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:4.75|P-value:2.58E-7 ID:SGTC_1520|Compound:flindersine|FD-Score:-3.57|P-value:1.08E-4 ID:SGTC_1534|Compound:6-azauridine|FD-Score:3.57|P-value:7.84E-5 ID:SGTC_1605|Compound:st000082|FD-Score:3.79|P-value:3.05E-5 ID:SGTC_1701|Compound:st028638|FD-Score:3.56|P-value:8.09E-5 ID:SGTC_1752|Compound:st044819|FD-Score:3.08|P-value:5.51E-4 ID:SGTC_1754|Compound:st042065|FD-Score:-3.11|P-value:6.40E-4 ID:SGTC_1800|Compound:st050079|FD-Score:-3.18|P-value:4.93E-4 ID:SGTC_1809|Compound:st050081|FD-Score:3.86|P-value:2.22E-5 ID:SGTC_1818|Compound:st052136|FD-Score:-3.77|P-value:4.60E-5 ID:SGTC_1821|Compound:st053465|FD-Score:3.99|P-value:1.22E-5 ID:SGTC_1847|Compound:st056231|FD-Score:-3.97|P-value:1.89E-5 ID:SGTC_1903|Compound:st060211|FD-Score:-3.23|P-value:4.15E-4 ID:SGTC_2010|Compound:4012422|FD-Score:2.96|P-value:8.53E-4 ID:SGTC_2059|Compound:5238311|FD-Score:4.48|P-value:1.11E-6 ID:SGTC_2099|Compound:5377090|FD-Score:3.28|P-value:2.58E-4 ID:SGTC_2193|Compound:5925765|FD-Score:3.24|P-value:2.93E-4 ID:SGTC_2195|Compound:6536160|FD-Score:3.72|P-value:4.13E-5 ID:SGTC_2200|Compound:6600604|FD-Score:2.93|P-value:9.47E-4 ID:SGTC_2314|Compound:7727897|FD-Score:4.85|P-value:1.49E-7 ID:SGTC_2348|Compound:9022543|FD-Score:3.68|P-value:4.93E-5 ID:SGTC_2390|Compound:9097792|FD-Score:4.30|P-value:2.77E-6 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.42|P-value:1.43E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:3.08|P-value:5.52E-4 ID:SGTC_2569|Compound:tetrandrine|FD-Score:3.86|P-value:2.20E-5 ID:SGTC_2793|Compound:7325131|FD-Score:-6.11|P-value:9.97E-11 ID:SGTC_2798|Compound:7813637|FD-Score:-3.59|P-value:9.87E-5 ID:SGTC_2817|Compound:7995342|FD-Score:3.19|P-value:3.52E-4 ID:SGTC_2888|Compound:9058547|FD-Score:3.12|P-value:4.73E-4 ID:SGTC_2934|Compound:9036370|FD-Score:-3.13|P-value:6.00E-4 ID:SGTC_3126|Compound:9127922|FD-Score:3.07|P-value:5.63E-4 ID:SGTC_3212|Compound:9128307|FD-Score:3.17|P-value:3.82E-4 ID:SGTC_3249|Compound:9135183|FD-Score:3.02|P-value:6.74E-4 ID:SGTC_3287|Compound:9117173|FD-Score:3.73|P-value:3.89E-5 ID:SGTC_3|Compound:0833-0587|FD-Score:3.01|P-value:6.91E-4 ID:SGTC_49|Compound:0717-0965|FD-Score:3.17|P-value:3.85E-4 ID:SGTC_105|Compound:0111-0055|FD-Score:3.12|P-value:4.63E-4 ID:SGTC_183|Compound:3914-0051|FD-Score:3.10|P-value:4.99E-4 ID:SGTC_246|Compound:cycloheximide|FD-Score:3.93|P-value:1.58E-5 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:3.51|P-value:9.89E-5 ID:SGTC_395|Compound:anisomycin|FD-Score:2.96|P-value:8.32E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:2.94|P-value:9.01E-4 ID:SGTC_744|Compound:1594-0068|FD-Score:4.78|P-value:2.25E-7 ID:SGTC_772|Compound:k072-0172|FD-Score:-3.14|P-value:5.72E-4 ID:SGTC_789|Compound:1309-1135|FD-Score:3.81|P-value:2.78E-5 ID:SGTC_876|Compound:0720-0963|FD-Score:3.25|P-value:2.86E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:3.19|P-value:3.57E-4 ID:SGTC_935|Compound:2996-0153|FD-Score:3.27|P-value:2.66E-4 ID:SGTC_937|Compound:3122-0005|FD-Score:3.03|P-value:6.51E-4 ID:SGTC_1015|Compound:4130-4525|FD-Score:3.89|P-value:1.96E-5 ID:SGTC_1057|Compound:3448-5381|FD-Score:-3.22|P-value:4.25E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.05|P-value:7.95E-4 ID:SGTC_1273|Compound:0849-0096|FD-Score:3.42|P-value:1.43E-4 ID:SGTC_1393|Compound:3470-5649|FD-Score:5.39|P-value:6.37E-9 ID:SGTC_1399|Compound:3562-5772|FD-Score:3.63|P-value:6.01E-5 ID:SGTC_1429|Compound:4049-0205|FD-Score:3.08|P-value:5.46E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:4.75|P-value:2.58E-7 ID:SGTC_1520|Compound:flindersine|FD-Score:-3.57|P-value:1.08E-4 ID:SGTC_1534|Compound:6-azauridine|FD-Score:3.57|P-value:7.84E-5 ID:SGTC_1605|Compound:st000082|FD-Score:3.79|P-value:3.05E-5 ID:SGTC_1701|Compound:st028638|FD-Score:3.56|P-value:8.09E-5 ID:SGTC_1752|Compound:st044819|FD-Score:3.08|P-value:5.51E-4 ID:SGTC_1754|Compound:st042065|FD-Score:-3.11|P-value:6.40E-4 ID:SGTC_1800|Compound:st050079|FD-Score:-3.18|P-value:4.93E-4 ID:SGTC_1809|Compound:st050081|FD-Score:3.86|P-value:2.22E-5 ID:SGTC_1818|Compound:st052136|FD-Score:-3.77|P-value:4.60E-5 ID:SGTC_1821|Compound:st053465|FD-Score:3.99|P-value:1.22E-5 ID:SGTC_1847|Compound:st056231|FD-Score:-3.97|P-value:1.89E-5 ID:SGTC_1903|Compound:st060211|FD-Score:-3.23|P-value:4.15E-4 ID:SGTC_2010|Compound:4012422|FD-Score:2.96|P-value:8.53E-4 ID:SGTC_2059|Compound:5238311|FD-Score:4.48|P-value:1.11E-6 ID:SGTC_2099|Compound:5377090|FD-Score:3.28|P-value:2.58E-4 ID:SGTC_2193|Compound:5925765|FD-Score:3.24|P-value:2.93E-4 ID:SGTC_2195|Compound:6536160|FD-Score:3.72|P-value:4.13E-5 ID:SGTC_2200|Compound:6600604|FD-Score:2.93|P-value:9.47E-4 ID:SGTC_2314|Compound:7727897|FD-Score:4.85|P-value:1.49E-7 ID:SGTC_2348|Compound:9022543|FD-Score:3.68|P-value:4.93E-5 ID:SGTC_2390|Compound:9097792|FD-Score:4.30|P-value:2.77E-6 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.42|P-value:1.43E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:3.08|P-value:5.52E-4 ID:SGTC_2569|Compound:tetrandrine|FD-Score:3.86|P-value:2.20E-5 ID:SGTC_2793|Compound:7325131|FD-Score:-6.11|P-value:9.97E-11 ID:SGTC_2798|Compound:7813637|FD-Score:-3.59|P-value:9.87E-5 ID:SGTC_2817|Compound:7995342|FD-Score:3.19|P-value:3.52E-4 ID:SGTC_2888|Compound:9058547|FD-Score:3.12|P-value:4.73E-4 ID:SGTC_2934|Compound:9036370|FD-Score:-3.13|P-value:6.00E-4 ID:SGTC_3126|Compound:9127922|FD-Score:3.07|P-value:5.63E-4 ID:SGTC_3212|Compound:9128307|FD-Score:3.17|P-value:3.82E-4 ID:SGTC_3249|Compound:9135183|FD-Score:3.02|P-value:6.74E-4 ID:SGTC_3287|Compound:9117173|FD-Score:3.73|P-value:3.89E-5

Top fitness defect scores for YPR189W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1393 3470-5649 5.39 58.2 µM 6.37E-9 60S ribosome export 4 3.50E-8
2 SGTC_2314 7727897 4.85 200.0 µM 1.49E-7 4 6.11E-7
3 SGTC_744 1594-0068 4.78 283.0 µM 2.25E-7 amide catabolism 13 8.87E-7
4 SGTC_1450 4112-4260 4.75 37.6 µM 2.58E-7 calcium & mitochondrial duress 16 1.00E-6
5 SGTC_2059 5238311 4.48 157.0 µM 1.11E-6 12 3.77E-6
6 SGTC_2390 9097792 4.30 176.6 µM 2.77E-6 8 8.65E-6
7 SGTC_1821 st053465 3.99 65.6 µM 1.22E-5 5 3.31E-5
8 SGTC_246 cycloheximide 3.93 667.0 µM 1.58E-5 14 4.18E-5
9 SGTC_1015 4130-4525 3.89 82.0 µM 1.96E-5 60S ribosome export 23 5.09E-5
10 SGTC_2569 tetrandrine 3.86 100.0 µM 2.20E-5 23 5.67E-5
11 SGTC_1809 st050081 3.86 57.6 µM 2.22E-5 16 5.71E-5
12 SGTC_789 1309-1135 3.81 70.6 µM 2.78E-5 ERG2 35 7.00E-5
13 SGTC_1605 st000082 3.79 49.1 µM 3.05E-5 60S ribosome export 19 7.61E-5
14 SGTC_3287 9117173 3.73 37.1 µM 3.89E-5 60S ribosome export 6 9.49E-5
15 SGTC_2195 6536160 3.72 144.2 µM 4.13E-5 60S ribosome export 15 1.00E-4
16 SGTC_2348 9022543 3.68 33.4 µM 4.93E-5 mitochondrial processes 34 1.18E-4
17 SGTC_1399 3562-5772 3.63 25.0 µM 6.01E-5 RPP1 & pyrimidine depletion 23 1.41E-4
18 SGTC_1534 6-azauridine 3.57 81.6 µM 7.84E-5 RNA pol III & RNase P/MRP 40 1.79E-4
19 SGTC_1701 st028638 3.56 40.4 µM 8.09E-5 24 1.84E-4
20 SGTC_265 mycophenolic acid 3.51 32.9 µM 9.89E-5 RPP1 & pyrimidine depletion 37 2.21E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.132 1.46E-14 YDL140C RPO21 het RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
0.085 7.19E-7 YJL218W_p YJL218W_p hom Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene
0.085 7.36E-7 YLR022C SDO1 het Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes
0.084 1.27E-6 YGR230W BNS1 hom Protein with some similarity to Spo12p; overexpression bypasses need for Spo12p, but not required for meiosis
0.076 1.04E-5 YPL167C REV3 hom Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair
0.071 4.32E-5 YLR307C-A_p YLR307C-A_p hom Putative protein of unknown function
0.069 7.06E-5 YDL105W NSE4 het Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
0.068 7.17E-5 YGL250W RMR1 hom Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus
0.068 7.49E-5 YHL003C LAG1 hom Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress
0.067 1.10E-4 YLL018C DPS1 het Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation
0.065 1.57E-4 YOL134C_d YOL134C_d het Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit
0.065 1.78E-4 YCR072C RSA4 het WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus
0.065 1.83E-4 YKR061W KTR2 hom Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication
0.064 2.17E-4 YPR178W PRP4 het Splicing factor, component of the U4/U6-U5 snRNP complex
0.063 2.68E-4 YMR175W-A_p YMR175W-A_p hom Putative protein of unknown function