YAL002W / VPS8

Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif

Zygosity: Homozygous strain
fixedexpanded
Profile for YAL002W / VPS8

Click on Significant Values for Screen Details ID:SGTC_1|Compound:0258-0098|FD-Score:3.56|P-value:2.77E-4 ID:SGTC_20|Compound:0481-0013|FD-Score:3.61|P-value:2.31E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:-4.25|P-value:2.22E-5 ID:SGTC_40|Compound:0327-0103|FD-Score:-9.25|P-value:2.83E-19 ID:SGTC_42|Compound:0133-0086|FD-Score:-3.89|P-value:9.57E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-7.21|P-value:2.05E-12 ID:SGTC_59|Compound:0330-0160|FD-Score:-4.27|P-value:2.09E-5 ID:SGTC_88|Compound:0180-0332|FD-Score:4.70|P-value:2.70E-6 ID:SGTC_117|Compound:lucigenin|FD-Score:6.43|P-value:2.50E-10 ID:SGTC_119|Compound:0121-0043|FD-Score:4.00|P-value:5.36E-5 ID:SGTC_124|Compound:0136-0217|FD-Score:4.20|P-value:2.36E-5 ID:SGTC_157|Compound:1348-1695|FD-Score:3.86|P-value:9.32E-5 ID:SGTC_178|Compound:4092-0806|FD-Score:-3.80|P-value:1.32E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:6.26|P-value:7.32E-10 ID:SGTC_263|Compound:alizarin|FD-Score:5.26|P-value:1.76E-7 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.27|P-value:8.49E-4 ID:SGTC_435|Compound:c-PAF|FD-Score:-3.52|P-value:3.69E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:3.35|P-value:5.77E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:4.60|P-value:4.21E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.79|P-value:3.43E-11 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:4.08|P-value:3.88E-5 ID:SGTC_525|Compound:k781-2675|FD-Score:3.22|P-value:8.87E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:8.12|P-value:2.24E-15 ID:SGTC_535|Compound:2293-4129|FD-Score:3.49|P-value:3.64E-4 ID:SGTC_608|Compound:k048-0125|FD-Score:3.54|P-value:2.97E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:4.76|P-value:2.04E-6 ID:SGTC_659|Compound:k018-0002|FD-Score:4.68|P-value:2.99E-6 ID:SGTC_785|Compound:1270-0111|FD-Score:3.68|P-value:1.83E-4 ID:SGTC_788|Compound:1182-0090|FD-Score:4.78|P-value:1.90E-6 ID:SGTC_847|Compound:0342-0274|FD-Score:5.32|P-value:1.36E-7 ID:SGTC_951|Compound:1171-0579|FD-Score:3.47|P-value:3.81E-4 ID:SGTC_1021|Compound:4476-0760|FD-Score:3.62|P-value:2.23E-4 ID:SGTC_1070|Compound:indatraline|FD-Score:3.54|P-value:2.99E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.43|P-value:4.48E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:4.73|P-value:2.40E-6 ID:SGTC_1256|Compound:0570-0116|FD-Score:3.99|P-value:5.59E-5 ID:SGTC_1260|Compound:0700-1617|FD-Score:3.83|P-value:1.04E-4 ID:SGTC_1306|Compound:1185-0270|FD-Score:8.60|P-value:5.06E-17 ID:SGTC_1430|Compound:4049-0248|FD-Score:3.20|P-value:9.49E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:3.31|P-value:6.73E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:7.69|P-value:5.25E-14 ID:SGTC_1547|Compound:harmine|FD-Score:3.41|P-value:4.77E-4 ID:SGTC_1635|Compound:st007035|FD-Score:3.90|P-value:7.79E-5 ID:SGTC_1658|Compound:st012944|FD-Score:5.51|P-value:5.03E-8 ID:SGTC_1660|Compound:st012960|FD-Score:4.01|P-value:5.17E-5 ID:SGTC_1687|Compound:st020778|FD-Score:7.18|P-value:2.00E-12 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.40|P-value:5.52E-4 ID:SGTC_1731|Compound:st035964|FD-Score:3.20|P-value:9.56E-4 ID:SGTC_1754|Compound:st042065|FD-Score:6.27|P-value:6.63E-10 ID:SGTC_1760|Compound:st045453|FD-Score:4.11|P-value:3.49E-5 ID:SGTC_1789|Compound:tunicamycin|FD-Score:4.03|P-value:4.68E-5 ID:SGTC_1800|Compound:st050079|FD-Score:5.32|P-value:1.34E-7 ID:SGTC_1801|Compound:st050089|FD-Score:4.35|P-value:1.26E-5 ID:SGTC_1803|Compound:st050068|FD-Score:8.36|P-value:3.26E-16 ID:SGTC_1877|Compound:wortmannin|FD-Score:4.49|P-value:6.98E-6 ID:SGTC_2021|Compound:4019055|FD-Score:3.74|P-value:1.44E-4 ID:SGTC_2022|Compound:5136654|FD-Score:3.75|P-value:1.41E-4 ID:SGTC_2138|Compound:5575593|FD-Score:4.17|P-value:2.73E-5 ID:SGTC_2155|Compound:5570423|FD-Score:-3.67|P-value:2.14E-4 ID:SGTC_2196|Compound:6573537|FD-Score:3.47|P-value:3.83E-4 ID:SGTC_2286|Compound:7945697|FD-Score:4.20|P-value:2.37E-5 ID:SGTC_2322|Compound:7413319|FD-Score:7.72|P-value:4.32E-14 ID:SGTC_2526|Compound:patulin|FD-Score:3.81|P-value:1.12E-4 ID:SGTC_2532|Compound:xanthone|FD-Score:5.61|P-value:2.87E-8 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:6.39|P-value:3.20E-10 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.79|P-value:1.10E-8 ID:SGTC_2586|Compound:curcumin|FD-Score:6.10|P-value:1.80E-9 ID:SGTC_2619|Compound:obliquin|FD-Score:8.66|P-value:2.97E-17 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.42|P-value:2.65E-10 ID:SGTC_2691|Compound:st077356|FD-Score:3.25|P-value:8.07E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:3.54|P-value:2.99E-4 ID:SGTC_2743|Compound:auranofin|FD-Score:4.44|P-value:8.72E-6 ID:SGTC_2815|Compound:7968870|FD-Score:4.31|P-value:1.48E-5 ID:SGTC_2942|Compound:9052777|FD-Score:-4.26|P-value:2.18E-5 ID:SGTC_2964|Compound:9083345|FD-Score:-4.93|P-value:1.12E-6 ID:SGTC_1|Compound:0258-0098|FD-Score:3.56|P-value:2.77E-4 ID:SGTC_20|Compound:0481-0013|FD-Score:3.61|P-value:2.31E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:-4.25|P-value:2.22E-5 ID:SGTC_40|Compound:0327-0103|FD-Score:-9.25|P-value:2.83E-19 ID:SGTC_42|Compound:0133-0086|FD-Score:-3.89|P-value:9.57E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-7.21|P-value:2.05E-12 ID:SGTC_59|Compound:0330-0160|FD-Score:-4.27|P-value:2.09E-5 ID:SGTC_88|Compound:0180-0332|FD-Score:4.70|P-value:2.70E-6 ID:SGTC_117|Compound:lucigenin|FD-Score:6.43|P-value:2.50E-10 ID:SGTC_119|Compound:0121-0043|FD-Score:4.00|P-value:5.36E-5 ID:SGTC_124|Compound:0136-0217|FD-Score:4.20|P-value:2.36E-5 ID:SGTC_157|Compound:1348-1695|FD-Score:3.86|P-value:9.32E-5 ID:SGTC_178|Compound:4092-0806|FD-Score:-3.80|P-value:1.32E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:6.26|P-value:7.32E-10 ID:SGTC_263|Compound:alizarin|FD-Score:5.26|P-value:1.76E-7 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.27|P-value:8.49E-4 ID:SGTC_435|Compound:c-PAF|FD-Score:-3.52|P-value:3.69E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:3.35|P-value:5.77E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:4.60|P-value:4.21E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.79|P-value:3.43E-11 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:4.08|P-value:3.88E-5 ID:SGTC_525|Compound:k781-2675|FD-Score:3.22|P-value:8.87E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:8.12|P-value:2.24E-15 ID:SGTC_535|Compound:2293-4129|FD-Score:3.49|P-value:3.64E-4 ID:SGTC_608|Compound:k048-0125|FD-Score:3.54|P-value:2.97E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:4.76|P-value:2.04E-6 ID:SGTC_659|Compound:k018-0002|FD-Score:4.68|P-value:2.99E-6 ID:SGTC_785|Compound:1270-0111|FD-Score:3.68|P-value:1.83E-4 ID:SGTC_788|Compound:1182-0090|FD-Score:4.78|P-value:1.90E-6 ID:SGTC_847|Compound:0342-0274|FD-Score:5.32|P-value:1.36E-7 ID:SGTC_951|Compound:1171-0579|FD-Score:3.47|P-value:3.81E-4 ID:SGTC_1021|Compound:4476-0760|FD-Score:3.62|P-value:2.23E-4 ID:SGTC_1070|Compound:indatraline|FD-Score:3.54|P-value:2.99E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.43|P-value:4.48E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:4.73|P-value:2.40E-6 ID:SGTC_1256|Compound:0570-0116|FD-Score:3.99|P-value:5.59E-5 ID:SGTC_1260|Compound:0700-1617|FD-Score:3.83|P-value:1.04E-4 ID:SGTC_1306|Compound:1185-0270|FD-Score:8.60|P-value:5.06E-17 ID:SGTC_1430|Compound:4049-0248|FD-Score:3.20|P-value:9.49E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:3.31|P-value:6.73E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:7.69|P-value:5.25E-14 ID:SGTC_1547|Compound:harmine|FD-Score:3.41|P-value:4.77E-4 ID:SGTC_1635|Compound:st007035|FD-Score:3.90|P-value:7.79E-5 ID:SGTC_1658|Compound:st012944|FD-Score:5.51|P-value:5.03E-8 ID:SGTC_1660|Compound:st012960|FD-Score:4.01|P-value:5.17E-5 ID:SGTC_1687|Compound:st020778|FD-Score:7.18|P-value:2.00E-12 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.40|P-value:5.52E-4 ID:SGTC_1731|Compound:st035964|FD-Score:3.20|P-value:9.56E-4 ID:SGTC_1754|Compound:st042065|FD-Score:6.27|P-value:6.63E-10 ID:SGTC_1760|Compound:st045453|FD-Score:4.11|P-value:3.49E-5 ID:SGTC_1789|Compound:tunicamycin|FD-Score:4.03|P-value:4.68E-5 ID:SGTC_1800|Compound:st050079|FD-Score:5.32|P-value:1.34E-7 ID:SGTC_1801|Compound:st050089|FD-Score:4.35|P-value:1.26E-5 ID:SGTC_1803|Compound:st050068|FD-Score:8.36|P-value:3.26E-16 ID:SGTC_1877|Compound:wortmannin|FD-Score:4.49|P-value:6.98E-6 ID:SGTC_2021|Compound:4019055|FD-Score:3.74|P-value:1.44E-4 ID:SGTC_2022|Compound:5136654|FD-Score:3.75|P-value:1.41E-4 ID:SGTC_2138|Compound:5575593|FD-Score:4.17|P-value:2.73E-5 ID:SGTC_2155|Compound:5570423|FD-Score:-3.67|P-value:2.14E-4 ID:SGTC_2196|Compound:6573537|FD-Score:3.47|P-value:3.83E-4 ID:SGTC_2286|Compound:7945697|FD-Score:4.20|P-value:2.37E-5 ID:SGTC_2322|Compound:7413319|FD-Score:7.72|P-value:4.32E-14 ID:SGTC_2526|Compound:patulin|FD-Score:3.81|P-value:1.12E-4 ID:SGTC_2532|Compound:xanthone|FD-Score:5.61|P-value:2.87E-8 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:6.39|P-value:3.20E-10 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.79|P-value:1.10E-8 ID:SGTC_2586|Compound:curcumin|FD-Score:6.10|P-value:1.80E-9 ID:SGTC_2619|Compound:obliquin|FD-Score:8.66|P-value:2.97E-17 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.42|P-value:2.65E-10 ID:SGTC_2691|Compound:st077356|FD-Score:3.25|P-value:8.07E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:3.54|P-value:2.99E-4 ID:SGTC_2743|Compound:auranofin|FD-Score:4.44|P-value:8.72E-6 ID:SGTC_2815|Compound:7968870|FD-Score:4.31|P-value:1.48E-5 ID:SGTC_2942|Compound:9052777|FD-Score:-4.26|P-value:2.18E-5 ID:SGTC_2964|Compound:9083345|FD-Score:-4.93|P-value:1.12E-6

Top fitness defect scores for YAL002W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2619 obliquin 8.66 87.9 µM 2.97E-17 cell wall 36 2.34E-18
2 SGTC_1306 1185-0270 8.60 9.0 µM 5.06E-17 Golgi 16 4.15E-18
3 SGTC_1803 st050068 8.36 52.4 µM 3.26E-16 NEO1-PIK1 35 3.06E-17
4 SGTC_528 3492-0019 8.12 172.0 µM 2.24E-15 calcium & mitochondrial duress 68 2.42E-16
5 SGTC_2322 7413319 7.72 38.2 µM 4.32E-14 ERAD & cell cycle 36 5.78E-15
6 SGTC_1495 4469-0029 7.69 237.0 µM 5.25E-14 27 7.12E-15
7 SGTC_1687 st020778 7.18 55.4 µM 2.00E-12 85 3.54E-13
8 SGTC_117 lucigenin 6.43 22.1 µM 2.50E-10 redox potentiating 35 6.26E-11
9 SGTC_2688 kanamycin b 6.42 96.7 µM 2.65E-10 calcium & mitochondrial duress 135 6.64E-11
10 SGTC_2567 aureobasidin a 6.39 60.0 nM 3.20E-10 38 8.12E-11
11 SGTC_1754 st042065 6.27 16.1 µM 6.63E-10 NEO1-PIK1 91 1.77E-10
12 SGTC_255 tunicamycin 6.26 200.5 nM 7.32E-10 unfolded protein response 30 1.97E-10
13 SGTC_2586 curcumin 6.10 80.0 µM 1.80E-9 iron homeostasis 14 5.18E-10
14 SGTC_2582 agaric acid 5.79 100.0 µM 1.10E-8 redox potentiating 172 3.61E-9
15 SGTC_2532 xanthone 5.61 78.9 µM 2.87E-8 9 1.00E-8
16 SGTC_1658 st012944 5.51 20.0 µM 5.03E-8 iron homeostasis 37 1.83E-8
17 SGTC_1800 st050079 5.32 29.1 µM 1.34E-7 NEO1-PIK1 66 5.24E-8
18 SGTC_847 0342-0274 5.32 5.5 µM 1.36E-7 7 5.31E-8
19 SGTC_263 alizarin 5.26 107.9 µM 1.76E-7 iron homeostasis 31 7.01E-8
20 SGTC_788 1182-0090 4.78 188.0 µM 1.90E-6 endomembrane recycling 58 8.91E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.438 3.91E-157 YOR089C VPS21 hom Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
0.385 1.47E-118 YDR495C VPS3 hom Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
0.378 1.41E-114 YDR323C PEP7 hom Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
0.376 6.40E-113 YML097C VPS9 hom A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin
0.366 1.62E-106 YOR036W PEP12 hom Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
0.319 2.76E-80 YPL065W VPS28 hom Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p
0.318 8.17E-80 YCL008C STP22 hom Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype
0.312 1.23E-76 YLR417W VPS36 hom Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome
0.311 5.43E-76 YGL212W VAM7 hom Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
0.304 1.24E-72 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.304 2.04E-72 YPL120W VPS30 hom Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1
0.297 4.29E-69 YGR206W MVB12 hom ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.289 9.17E-66 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.288 7.03E-65 YJR102C VPS25 hom Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome
0.284 1.80E-63 YDR455C_d YDR455C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W