YAL015C / NTG1

DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YAL015C / NTG1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:14.60|P-value:1.43E-52 ID:SGTC_67|Compound:0335-0847|FD-Score:3.27|P-value:2.89E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:3.02|P-value:7.45E-4 ID:SGTC_78|Compound:0336-0123|FD-Score:-3.13|P-value:5.85E-4 ID:SGTC_80|Compound:0159-0065|FD-Score:3.55|P-value:9.51E-5 ID:SGTC_86|Compound:0417-1630|FD-Score:4.81|P-value:2.23E-7 ID:SGTC_138|Compound:0850-0259|FD-Score:8.05|P-value:1.88E-17 ID:SGTC_142|Compound:0335-0849|FD-Score:4.12|P-value:7.67E-6 ID:SGTC_145|Compound:3737-0113|FD-Score:-4.34|P-value:3.26E-6 ID:SGTC_157|Compound:1348-1695|FD-Score:3.48|P-value:1.26E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:5.86|P-value:4.18E-10 ID:SGTC_294|Compound:k038-0071|FD-Score:3.45|P-value:1.46E-4 ID:SGTC_305|Compound:1682-2595|FD-Score:3.12|P-value:5.14E-4 ID:SGTC_993|Compound:3909-8757|FD-Score:-3.08|P-value:7.13E-4 ID:SGTC_1039|Compound:k821-0043|FD-Score:3.72|P-value:4.76E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.17|P-value:5.12E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:-3.14|P-value:5.65E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:3.74|P-value:4.22E-5 ID:SGTC_1493|Compound:4348-0038|FD-Score:3.47|P-value:1.32E-4 ID:SGTC_1582|Compound:3',3',6'-trihydroxyflavone|FD-Score:-3.57|P-value:1.09E-4 ID:SGTC_1594|Compound:piperine|FD-Score:-4.62|P-value:8.30E-7 ID:SGTC_1605|Compound:st000082|FD-Score:-3.84|P-value:3.48E-5 ID:SGTC_1608|Compound:st001473|FD-Score:-3.03|P-value:8.67E-4 ID:SGTC_1736|Compound:st036774|FD-Score:3.45|P-value:1.46E-4 ID:SGTC_1739|Compound:st037196|FD-Score:4.83|P-value:2.03E-7 ID:SGTC_1741|Compound:st038134|FD-Score:-4.83|P-value:2.62E-7 ID:SGTC_1743|Compound:st037606|FD-Score:-3.38|P-value:2.35E-4 ID:SGTC_2111|Compound:5486399|FD-Score:4.67|P-value:4.97E-7 ID:SGTC_2346|Compound:9031566|FD-Score:3.08|P-value:6.02E-4 ID:SGTC_2483|Compound:5729050|FD-Score:3.53|P-value:1.06E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.50|P-value:1.16E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:4.00|P-value:1.36E-5 ID:SGTC_2791|Compound:5663188|FD-Score:3.22|P-value:3.58E-4 ID:SGTC_2810|Compound:7959871|FD-Score:-3.27|P-value:3.56E-4 ID:SGTC_3067|Compound:9117431|FD-Score:-3.54|P-value:1.19E-4 ID:SGTC_3077|Compound:9117319|FD-Score:-4.36|P-value:2.96E-6 ID:SGTC_3191|Compound:9110352|FD-Score:-3.55|P-value:1.16E-4 ID:SGTC_3206|Compound:9114350|FD-Score:-3.03|P-value:8.43E-4 ID:SGTC_3321|Compound:9137793|FD-Score:3.04|P-value:7.00E-4 ID:SGTC_3336|Compound:9143619|FD-Score:3.66|P-value:6.11E-5 ID:SGTC_3342|Compound:9147791|FD-Score:3.28|P-value:2.80E-4 ID:SGTC_3354|Compound:9151612|FD-Score:3.51|P-value:1.14E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:14.60|P-value:1.43E-52 ID:SGTC_67|Compound:0335-0847|FD-Score:3.27|P-value:2.89E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:3.02|P-value:7.45E-4 ID:SGTC_78|Compound:0336-0123|FD-Score:-3.13|P-value:5.85E-4 ID:SGTC_80|Compound:0159-0065|FD-Score:3.55|P-value:9.51E-5 ID:SGTC_86|Compound:0417-1630|FD-Score:4.81|P-value:2.23E-7 ID:SGTC_138|Compound:0850-0259|FD-Score:8.05|P-value:1.88E-17 ID:SGTC_142|Compound:0335-0849|FD-Score:4.12|P-value:7.67E-6 ID:SGTC_145|Compound:3737-0113|FD-Score:-4.34|P-value:3.26E-6 ID:SGTC_157|Compound:1348-1695|FD-Score:3.48|P-value:1.26E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:5.86|P-value:4.18E-10 ID:SGTC_294|Compound:k038-0071|FD-Score:3.45|P-value:1.46E-4 ID:SGTC_305|Compound:1682-2595|FD-Score:3.12|P-value:5.14E-4 ID:SGTC_993|Compound:3909-8757|FD-Score:-3.08|P-value:7.13E-4 ID:SGTC_1039|Compound:k821-0043|FD-Score:3.72|P-value:4.76E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.17|P-value:5.12E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:-3.14|P-value:5.65E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:3.74|P-value:4.22E-5 ID:SGTC_1493|Compound:4348-0038|FD-Score:3.47|P-value:1.32E-4 ID:SGTC_1582|Compound:3',3',6'-trihydroxyflavone|FD-Score:-3.57|P-value:1.09E-4 ID:SGTC_1594|Compound:piperine|FD-Score:-4.62|P-value:8.30E-7 ID:SGTC_1605|Compound:st000082|FD-Score:-3.84|P-value:3.48E-5 ID:SGTC_1608|Compound:st001473|FD-Score:-3.03|P-value:8.67E-4 ID:SGTC_1736|Compound:st036774|FD-Score:3.45|P-value:1.46E-4 ID:SGTC_1739|Compound:st037196|FD-Score:4.83|P-value:2.03E-7 ID:SGTC_1741|Compound:st038134|FD-Score:-4.83|P-value:2.62E-7 ID:SGTC_1743|Compound:st037606|FD-Score:-3.38|P-value:2.35E-4 ID:SGTC_2111|Compound:5486399|FD-Score:4.67|P-value:4.97E-7 ID:SGTC_2346|Compound:9031566|FD-Score:3.08|P-value:6.02E-4 ID:SGTC_2483|Compound:5729050|FD-Score:3.53|P-value:1.06E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.50|P-value:1.16E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:4.00|P-value:1.36E-5 ID:SGTC_2791|Compound:5663188|FD-Score:3.22|P-value:3.58E-4 ID:SGTC_2810|Compound:7959871|FD-Score:-3.27|P-value:3.56E-4 ID:SGTC_3067|Compound:9117431|FD-Score:-3.54|P-value:1.19E-4 ID:SGTC_3077|Compound:9117319|FD-Score:-4.36|P-value:2.96E-6 ID:SGTC_3191|Compound:9110352|FD-Score:-3.55|P-value:1.16E-4 ID:SGTC_3206|Compound:9114350|FD-Score:-3.03|P-value:8.43E-4 ID:SGTC_3321|Compound:9137793|FD-Score:3.04|P-value:7.00E-4 ID:SGTC_3336|Compound:9143619|FD-Score:3.66|P-value:6.11E-5 ID:SGTC_3342|Compound:9147791|FD-Score:3.28|P-value:2.80E-4 ID:SGTC_3354|Compound:9151612|FD-Score:3.51|P-value:1.14E-4

Top fitness defect scores for YAL015C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 14.60 9.0 µM 1.43E-52 cell wall 28 2.68E-48
2 SGTC_138 0850-0259 8.05 40.9 µM 1.88E-17 cell wall 61 4.30E-16
3 SGTC_158 k038-0087 5.86 26.4 µM 4.18E-10 ubiquinone biosynthesis & proteasome 71 2.34E-9
4 SGTC_1739 st037196 4.83 92.5 µM 2.03E-7 6 6.80E-7
5 SGTC_86 0417-1630 4.81 471.9 µM 2.23E-7 ubiquinone biosynthesis & proteasome 116 7.40E-7
6 SGTC_2111 5486399 4.67 200.0 µM 4.97E-7 25 1.54E-6
7 SGTC_142 0335-0849 4.12 62.7 µM 7.67E-6 ubiquinone biosynthesis & proteasome 117 1.90E-5
8 SGTC_2638 epoxomicin 4.00 18.0 µM 1.36E-5 36 3.20E-5
9 SGTC_1248 0469-0687 3.74 58.6 µM 4.22E-5 35 9.06E-5
10 SGTC_1039 k821-0043 3.72 94.0 µM 4.76E-5 Golgi 32 1.01E-4
11 SGTC_3336 9143619 3.66 71.4 µM 6.11E-5 25 1.27E-4
12 SGTC_80 0159-0065 3.55 512.2 µM 9.51E-5 5 1.91E-4
13 SGTC_2483 5729050 3.53 27.2 µM 1.06E-4 37 2.11E-4
14 SGTC_3354 9151612 3.51 71.4 µM 1.14E-4 24 2.26E-4
15 SGTC_2582 agaric acid 3.50 100.0 µM 1.16E-4 redox potentiating 365 2.29E-4
16 SGTC_157 1348-1695 3.48 65.8 µM 1.26E-4 iron homeostasis 101 2.47E-4
17 SGTC_1493 4348-0038 3.47 111.0 µM 1.32E-4 RSC complex & mRNA processing 47 2.58E-4
18 SGTC_294 k038-0071 3.45 157.0 µM 1.46E-4 ubiquinone biosynthesis & proteasome 82 2.83E-4
19 SGTC_1736 st036774 3.45 51.0 µM 1.46E-4 54 2.83E-4
20 SGTC_3342 9147791 3.28 14.5 µM 2.80E-4 39 5.15E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.407 7.39E-134 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.394 3.95E-125 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.392 1.59E-123 YDR537C_d YDR537C_d hom Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W
0.377 7.07E-114 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.369 1.96E-108 YJL193W_p YJL193W_p hom Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect
0.359 8.95E-103 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.352 3.09E-98 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.329 1.18E-85 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.324 9.28E-83 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.323 2.22E-82 YJL155C FBP26 hom Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress
0.322 7.21E-82 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.318 1.01E-79 YDR335W MSN5 hom Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm
0.314 9.46E-78 YPL269W KAR9 hom Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase
0.311 6.05E-76 YKR042W UTH1 hom Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication
0.310 8.29E-76 YML057C-A_d YML057C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W