YAL025C / MAK16

Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus

Zygosity: Heterozygous strain
fixedexpanded
Profile for YAL025C / MAK16

Click on Significant Values for Screen Details ID:SGTC_7|Compound:0192-1582|FD-Score:3.37|P-value:3.46E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:-3.21|P-value:5.32E-4 ID:SGTC_258|Compound:haloperidol|FD-Score:3.29|P-value:4.66E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:3.48|P-value:2.32E-4 ID:SGTC_403|Compound:arphamenine a|FD-Score:-3.11|P-value:7.80E-4 ID:SGTC_531|Compound:3788-1495|FD-Score:-3.35|P-value:3.20E-4 ID:SGTC_596|Compound:1155-0152|FD-Score:3.18|P-value:6.86E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:3.96|P-value:3.27E-5 ID:SGTC_852|Compound:0418-0143|FD-Score:-5.13|P-value:9.44E-8 ID:SGTC_879|Compound:0831-1030|FD-Score:3.76|P-value:7.74E-5 ID:SGTC_1074|Compound:carmofur|FD-Score:3.12|P-value:8.48E-4 ID:SGTC_1229|Compound:0139-0193|FD-Score:-3.78|P-value:6.12E-5 ID:SGTC_1255|Compound:0566-0045|FD-Score:-3.07|P-value:8.72E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:3.27|P-value:5.04E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:5.01|P-value:2.21E-7 ID:SGTC_1456|Compound:k015-0012|FD-Score:5.65|P-value:5.90E-9 ID:SGTC_1668|Compound:st014914|FD-Score:3.72|P-value:9.05E-5 ID:SGTC_1708|Compound:st033234|FD-Score:3.40|P-value:3.06E-4 ID:SGTC_1750|Compound:st081859|FD-Score:3.30|P-value:4.51E-4 ID:SGTC_1769|Compound:st044406|FD-Score:3.50|P-value:2.11E-4 ID:SGTC_1770|Compound:st043884|FD-Score:3.58|P-value:1.54E-4 ID:SGTC_1776|Compound:st049249|FD-Score:4.44|P-value:3.93E-6 ID:SGTC_1786|Compound:berberine|FD-Score:-4.40|P-value:3.93E-6 ID:SGTC_1855|Compound:st056195|FD-Score:-3.42|P-value:2.48E-4 ID:SGTC_1909|Compound:st060837|FD-Score:-3.41|P-value:2.55E-4 ID:SGTC_1915|Compound:st067105|FD-Score:-3.15|P-value:6.69E-4 ID:SGTC_2128|Compound:5255449|FD-Score:3.18|P-value:6.88E-4 ID:SGTC_2171|Compound:5739187|FD-Score:3.52|P-value:2.00E-4 ID:SGTC_2338|Compound:7999974|FD-Score:-3.82|P-value:5.17E-5 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:3.34|P-value:3.94E-4 ID:SGTC_2433|Compound:5607874|FD-Score:3.55|P-value:1.77E-4 ID:SGTC_2479|Compound:5617353|FD-Score:3.30|P-value:4.51E-4 ID:SGTC_2511|Compound:berberine|FD-Score:-3.43|P-value:2.43E-4 ID:SGTC_2546|Compound:rhodinyl acetate|FD-Score:-3.12|P-value:7.45E-4 ID:SGTC_2750|Compound:bromebric acid|FD-Score:3.76|P-value:7.56E-5 ID:SGTC_2757|Compound:clomiphene|FD-Score:-3.30|P-value:3.94E-4 ID:SGTC_2788|Compound:5282734|FD-Score:3.87|P-value:4.97E-5 ID:SGTC_2955|Compound:9076254|FD-Score:3.14|P-value:8.05E-4 ID:SGTC_3081|Compound:9117243|FD-Score:3.59|P-value:1.54E-4 ID:SGTC_3129|Compound:9127257|FD-Score:3.38|P-value:3.38E-4 ID:SGTC_3205|Compound:9113483|FD-Score:3.08|P-value:9.86E-4 ID:SGTC_7|Compound:0192-1582|FD-Score:3.37|P-value:3.46E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:-3.21|P-value:5.32E-4 ID:SGTC_258|Compound:haloperidol|FD-Score:3.29|P-value:4.66E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:3.48|P-value:2.32E-4 ID:SGTC_403|Compound:arphamenine a|FD-Score:-3.11|P-value:7.80E-4 ID:SGTC_531|Compound:3788-1495|FD-Score:-3.35|P-value:3.20E-4 ID:SGTC_596|Compound:1155-0152|FD-Score:3.18|P-value:6.86E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:3.96|P-value:3.27E-5 ID:SGTC_852|Compound:0418-0143|FD-Score:-5.13|P-value:9.44E-8 ID:SGTC_879|Compound:0831-1030|FD-Score:3.76|P-value:7.74E-5 ID:SGTC_1074|Compound:carmofur|FD-Score:3.12|P-value:8.48E-4 ID:SGTC_1229|Compound:0139-0193|FD-Score:-3.78|P-value:6.12E-5 ID:SGTC_1255|Compound:0566-0045|FD-Score:-3.07|P-value:8.72E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:3.27|P-value:5.04E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:5.01|P-value:2.21E-7 ID:SGTC_1456|Compound:k015-0012|FD-Score:5.65|P-value:5.90E-9 ID:SGTC_1668|Compound:st014914|FD-Score:3.72|P-value:9.05E-5 ID:SGTC_1708|Compound:st033234|FD-Score:3.40|P-value:3.06E-4 ID:SGTC_1750|Compound:st081859|FD-Score:3.30|P-value:4.51E-4 ID:SGTC_1769|Compound:st044406|FD-Score:3.50|P-value:2.11E-4 ID:SGTC_1770|Compound:st043884|FD-Score:3.58|P-value:1.54E-4 ID:SGTC_1776|Compound:st049249|FD-Score:4.44|P-value:3.93E-6 ID:SGTC_1786|Compound:berberine|FD-Score:-4.40|P-value:3.93E-6 ID:SGTC_1855|Compound:st056195|FD-Score:-3.42|P-value:2.48E-4 ID:SGTC_1909|Compound:st060837|FD-Score:-3.41|P-value:2.55E-4 ID:SGTC_1915|Compound:st067105|FD-Score:-3.15|P-value:6.69E-4 ID:SGTC_2128|Compound:5255449|FD-Score:3.18|P-value:6.88E-4 ID:SGTC_2171|Compound:5739187|FD-Score:3.52|P-value:2.00E-4 ID:SGTC_2338|Compound:7999974|FD-Score:-3.82|P-value:5.17E-5 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:3.34|P-value:3.94E-4 ID:SGTC_2433|Compound:5607874|FD-Score:3.55|P-value:1.77E-4 ID:SGTC_2479|Compound:5617353|FD-Score:3.30|P-value:4.51E-4 ID:SGTC_2511|Compound:berberine|FD-Score:-3.43|P-value:2.43E-4 ID:SGTC_2546|Compound:rhodinyl acetate|FD-Score:-3.12|P-value:7.45E-4 ID:SGTC_2750|Compound:bromebric acid|FD-Score:3.76|P-value:7.56E-5 ID:SGTC_2757|Compound:clomiphene|FD-Score:-3.30|P-value:3.94E-4 ID:SGTC_2788|Compound:5282734|FD-Score:3.87|P-value:4.97E-5 ID:SGTC_2955|Compound:9076254|FD-Score:3.14|P-value:8.05E-4 ID:SGTC_3081|Compound:9117243|FD-Score:3.59|P-value:1.54E-4 ID:SGTC_3129|Compound:9127257|FD-Score:3.38|P-value:3.38E-4 ID:SGTC_3205|Compound:9113483|FD-Score:3.08|P-value:9.86E-4

Top fitness defect scores for YAL025C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1456 k015-0012 5.65 13.0 µM 5.90E-9 1 7.84E-9 0.71
2 SGTC_1454 4239-0054 5.01 42.6 µM 2.21E-7 3 2.74E-7 0.62
3 SGTC_1776 st049249 4.44 45.9 µM 3.93E-6 1 4.60E-6 0.38
4 SGTC_610 4215-0184 3.96 155.0 µM 3.27E-5 exosome 25 3.68E-5 0.19
5 SGTC_2788 5282734 3.87 71.4 µM 4.97E-5 9 5.55E-5 0.17
6 SGTC_2750 bromebric acid 3.76 70.2 µM 7.56E-5 6 8.38E-5 0.05
7 SGTC_879 0831-1030 3.76 162.0 µM 7.74E-5 25 8.57E-5 0.07
8 SGTC_1668 st014914 3.72 51.6 µM 9.05E-5 RPP1 & pyrimidine depletion 7 1.00E-4 0.28
9 SGTC_3081 9117243 3.59 49.5 µM 1.54E-4 fatty acid desaturase (OLE1) 34 1.68E-4 0.04
10 SGTC_1770 st043884 3.58 66.3 µM 1.54E-4 7 1.69E-4 0.06
11 SGTC_2433 5607874 3.55 27.8 µM 1.77E-4 5 1.93E-4 0.06
12 SGTC_2171 5739187 3.52 198.1 µM 2.00E-4 copper-dependent oxidative stress 6 2.18E-4 0.12
13 SGTC_1769 st044406 3.50 64.4 µM 2.11E-4 15 2.30E-4 0.00
14 SGTC_273 hydroxyurea 3.48 18.1 mM 2.32E-4 heme biosynthesis & mitochondrial translocase 29 2.52E-4 0.04
15 SGTC_1708 st033234 3.40 31.8 µM 3.06E-4 8 3.31E-4 0.14
16 SGTC_3129 9127257 3.38 49.5 µM 3.38E-4 11 3.65E-4 0.03
17 SGTC_7 0192-1582 3.37 4.2 µM 3.46E-4 9 3.73E-4 0.01
18 SGTC_2392 N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide 3.34 200.0 µM 3.94E-4 12 4.24E-4 0.03
19 SGTC_2479 5617353 3.30 43.9 µM 4.51E-4 16 4.84E-4 0.02
20 SGTC_1750 st081859 3.30 2.4 µM 4.51E-4 endomembrane recycling 36 4.85E-4 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.135 4.26E-15 YOL021C DIS3 het Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
0.128 1.04E-13 YGR195W SKI6 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
0.121 1.63E-12 YJL078C PRY3 hom Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p
0.118 7.66E-12 YNL002C RLP7 het Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
0.116 1.47E-11 YJL027C_p YJL027C_p hom Putative protein of unknown function
0.113 4.46E-11 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.111 1.36E-10 YNL061W NOP2 het Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles
0.106 6.23E-10 YGR158C MTR3 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
0.104 1.66E-9 YPR143W RRP15 het Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
0.098 1.36E-8 YOL120C RPL18A het Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
0.095 3.33E-8 YNL232W CSL4 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)
0.093 6.24E-8 YPR029C APL4 hom Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
0.093 7.20E-8 YPR153W YPR153W hom Putative protein of unknown function
0.092 8.30E-8 YDR047W HEM12 het Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
0.091 1.27E-7 YLL025W PAU17 hom Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene