YAL026C / DRS2

Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease

Zygosity: Homozygous strain
fixedexpanded
Profile for YAL026C / DRS2

Click on Significant Values for Screen Details ID:SGTC_20|Compound:0481-0013|FD-Score:3.74|P-value:3.82E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:7.54|P-value:6.58E-12 ID:SGTC_58|Compound:0957-0393|FD-Score:4.57|P-value:1.95E-5 ID:SGTC_86|Compound:0417-1630|FD-Score:3.92|P-value:2.08E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:4.44|P-value:3.24E-5 ID:SGTC_142|Compound:0335-0849|FD-Score:4.54|P-value:2.20E-5 ID:SGTC_156|Compound:3474-0145|FD-Score:3.96|P-value:1.81E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.57|P-value:6.36E-4 ID:SGTC_172|Compound:1112-0046|FD-Score:5.65|P-value:1.94E-7 ID:SGTC_178|Compound:4092-0806|FD-Score:4.57|P-value:1.96E-5 ID:SGTC_181|Compound:1447-1644|FD-Score:5.44|P-value:5.09E-7 ID:SGTC_207|Compound:4185-0010|FD-Score:3.89|P-value:2.29E-4 ID:SGTC_258|Compound:haloperidol|FD-Score:5.06|P-value:2.64E-6 ID:SGTC_361|Compound:000s-0510|FD-Score:-7.53|P-value:1.03E-11 ID:SGTC_367|Compound:0335-0881|FD-Score:10.30|P-value:8.65E-21 ID:SGTC_372|Compound:0155-0182|FD-Score:7.92|P-value:6.00E-13 ID:SGTC_373|Compound:0844-0104|FD-Score:4.12|P-value:1.04E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:4.36|P-value:4.42E-5 ID:SGTC_449|Compound:xestospongin c|FD-Score:3.93|P-value:2.04E-4 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:4.01|P-value:1.54E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:3.87|P-value:2.42E-4 ID:SGTC_577|Compound:r071-0003|FD-Score:3.98|P-value:1.69E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:4.45|P-value:3.05E-5 ID:SGTC_637|Compound:1181-0519|FD-Score:6.44|P-value:3.74E-9 ID:SGTC_658|Compound:3253-0951|FD-Score:3.46|P-value:9.05E-4 ID:SGTC_664|Compound:1222-0054|FD-Score:4.39|P-value:3.90E-5 ID:SGTC_794|Compound:k038-0031|FD-Score:4.31|P-value:5.20E-5 ID:SGTC_813|Compound:k007-0158|FD-Score:4.11|P-value:1.09E-4 ID:SGTC_834|Compound:0312-0008|FD-Score:4.30|P-value:5.35E-5 ID:SGTC_837|Compound:0091-0265|FD-Score:3.99|P-value:1.62E-4 ID:SGTC_868|Compound:0527-0199|FD-Score:3.99|P-value:1.62E-4 ID:SGTC_950|Compound:1165-0508|FD-Score:5.07|P-value:2.55E-6 ID:SGTC_954|Compound:1189-0853|FD-Score:7.30|P-value:2.92E-11 ID:SGTC_1019|Compound:4262-0212|FD-Score:4.29|P-value:5.68E-5 ID:SGTC_1026|Compound:k007-1105|FD-Score:6.83|P-value:4.44E-10 ID:SGTC_1053|Compound:3366-3204|FD-Score:3.45|P-value:9.36E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.53|P-value:7.29E-4 ID:SGTC_1076|Compound:itraconazole|FD-Score:3.67|P-value:4.73E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-5.96|P-value:5.73E-8 ID:SGTC_1111|Compound:tnp00062|FD-Score:-4.89|P-value:6.95E-6 ID:SGTC_1130|Compound:3454-2858|FD-Score:4.79|P-value:8.31E-6 ID:SGTC_1160|Compound:4092-0570|FD-Score:5.76|P-value:1.11E-7 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.77|P-value:3.42E-4 ID:SGTC_1241|Compound:0364-0022|FD-Score:-3.53|P-value:8.71E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:4.21|P-value:7.53E-5 ID:SGTC_1334|Compound:1486-0610|FD-Score:3.90|P-value:2.23E-4 ID:SGTC_1394|Compound:3474-0001|FD-Score:4.01|P-value:1.50E-4 ID:SGTC_1475|Compound:4092-1048|FD-Score:5.52|P-value:3.51E-7 ID:SGTC_1660|Compound:st012960|FD-Score:4.46|P-value:2.94E-5 ID:SGTC_1672|Compound:st016598|FD-Score:5.90|P-value:5.90E-8 ID:SGTC_1757|Compound:st041620|FD-Score:5.15|P-value:1.87E-6 ID:SGTC_1760|Compound:st045453|FD-Score:3.86|P-value:2.53E-4 ID:SGTC_1781|Compound:st038725|FD-Score:7.29|P-value:3.03E-11 ID:SGTC_1797|Compound:st048695|FD-Score:3.63|P-value:5.35E-4 ID:SGTC_1806|Compound:st050005|FD-Score:3.49|P-value:8.22E-4 ID:SGTC_1874|Compound:staurosporine|FD-Score:3.66|P-value:4.92E-4 ID:SGTC_1944|Compound:st076371|FD-Score:5.63|P-value:2.15E-7 ID:SGTC_1949|Compound:st076024|FD-Score:3.63|P-value:5.35E-4 ID:SGTC_1952|Compound:st077036|FD-Score:4.14|P-value:9.71E-5 ID:SGTC_1954|Compound:st077032|FD-Score:4.12|P-value:1.05E-4 ID:SGTC_1991|Compound:st073000|FD-Score:7.50|P-value:8.38E-12 ID:SGTC_1999|Compound:st074831|FD-Score:3.76|P-value:3.58E-4 ID:SGTC_2006|Compound:4011487|FD-Score:3.55|P-value:6.87E-4 ID:SGTC_2028|Compound:5153890|FD-Score:3.55|P-value:6.91E-4 ID:SGTC_2320|Compound:7373003|FD-Score:3.75|P-value:3.58E-4 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:-14.40|P-value:7.09E-38 ID:SGTC_2699|Compound:st077704|FD-Score:7.26|P-value:3.53E-11 ID:SGTC_2721|Compound:ifenprodil|FD-Score:3.82|P-value:2.91E-4 ID:SGTC_2790|Compound:5423970|FD-Score:4.23|P-value:6.98E-5 ID:SGTC_2792|Compound:7535235|FD-Score:3.78|P-value:3.34E-4 ID:SGTC_2812|Compound:7970281|FD-Score:4.36|P-value:4.31E-5 ID:SGTC_2816|Compound:7982706|FD-Score:3.71|P-value:4.18E-4 ID:SGTC_2887|Compound:9044784|FD-Score:4.76|P-value:9.12E-6 ID:SGTC_2938|Compound:9059746|FD-Score:4.68|P-value:1.29E-5 ID:SGTC_2948|Compound:9070189|FD-Score:4.66|P-value:1.37E-5 ID:SGTC_2965|Compound:9082772|FD-Score:3.64|P-value:5.21E-4 ID:SGTC_3054|Compound:9094397|FD-Score:3.97|P-value:1.73E-4 ID:SGTC_3071|Compound:9117417|FD-Score:4.27|P-value:6.07E-5 ID:SGTC_3081|Compound:9117243|FD-Score:7.63|P-value:3.71E-12 ID:SGTC_3126|Compound:9127922|FD-Score:3.66|P-value:4.79E-4 ID:SGTC_3143|Compound:9086658|FD-Score:-4.05|P-value:1.63E-4 ID:SGTC_3218|Compound:9131285|FD-Score:5.05|P-value:2.81E-6 ID:SGTC_3222|Compound:9128301|FD-Score:-3.68|P-value:5.45E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-4.06|P-value:1.56E-4 ID:SGTC_20|Compound:0481-0013|FD-Score:3.74|P-value:3.82E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:7.54|P-value:6.58E-12 ID:SGTC_58|Compound:0957-0393|FD-Score:4.57|P-value:1.95E-5 ID:SGTC_86|Compound:0417-1630|FD-Score:3.92|P-value:2.08E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:4.44|P-value:3.24E-5 ID:SGTC_142|Compound:0335-0849|FD-Score:4.54|P-value:2.20E-5 ID:SGTC_156|Compound:3474-0145|FD-Score:3.96|P-value:1.81E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.57|P-value:6.36E-4 ID:SGTC_172|Compound:1112-0046|FD-Score:5.65|P-value:1.94E-7 ID:SGTC_178|Compound:4092-0806|FD-Score:4.57|P-value:1.96E-5 ID:SGTC_181|Compound:1447-1644|FD-Score:5.44|P-value:5.09E-7 ID:SGTC_207|Compound:4185-0010|FD-Score:3.89|P-value:2.29E-4 ID:SGTC_258|Compound:haloperidol|FD-Score:5.06|P-value:2.64E-6 ID:SGTC_361|Compound:000s-0510|FD-Score:-7.53|P-value:1.03E-11 ID:SGTC_367|Compound:0335-0881|FD-Score:10.30|P-value:8.65E-21 ID:SGTC_372|Compound:0155-0182|FD-Score:7.92|P-value:6.00E-13 ID:SGTC_373|Compound:0844-0104|FD-Score:4.12|P-value:1.04E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:4.36|P-value:4.42E-5 ID:SGTC_449|Compound:xestospongin c|FD-Score:3.93|P-value:2.04E-4 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:4.01|P-value:1.54E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:3.87|P-value:2.42E-4 ID:SGTC_577|Compound:r071-0003|FD-Score:3.98|P-value:1.69E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:4.45|P-value:3.05E-5 ID:SGTC_637|Compound:1181-0519|FD-Score:6.44|P-value:3.74E-9 ID:SGTC_658|Compound:3253-0951|FD-Score:3.46|P-value:9.05E-4 ID:SGTC_664|Compound:1222-0054|FD-Score:4.39|P-value:3.90E-5 ID:SGTC_794|Compound:k038-0031|FD-Score:4.31|P-value:5.20E-5 ID:SGTC_813|Compound:k007-0158|FD-Score:4.11|P-value:1.09E-4 ID:SGTC_834|Compound:0312-0008|FD-Score:4.30|P-value:5.35E-5 ID:SGTC_837|Compound:0091-0265|FD-Score:3.99|P-value:1.62E-4 ID:SGTC_868|Compound:0527-0199|FD-Score:3.99|P-value:1.62E-4 ID:SGTC_950|Compound:1165-0508|FD-Score:5.07|P-value:2.55E-6 ID:SGTC_954|Compound:1189-0853|FD-Score:7.30|P-value:2.92E-11 ID:SGTC_1019|Compound:4262-0212|FD-Score:4.29|P-value:5.68E-5 ID:SGTC_1026|Compound:k007-1105|FD-Score:6.83|P-value:4.44E-10 ID:SGTC_1053|Compound:3366-3204|FD-Score:3.45|P-value:9.36E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.53|P-value:7.29E-4 ID:SGTC_1076|Compound:itraconazole|FD-Score:3.67|P-value:4.73E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-5.96|P-value:5.73E-8 ID:SGTC_1111|Compound:tnp00062|FD-Score:-4.89|P-value:6.95E-6 ID:SGTC_1130|Compound:3454-2858|FD-Score:4.79|P-value:8.31E-6 ID:SGTC_1160|Compound:4092-0570|FD-Score:5.76|P-value:1.11E-7 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.77|P-value:3.42E-4 ID:SGTC_1241|Compound:0364-0022|FD-Score:-3.53|P-value:8.71E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:4.21|P-value:7.53E-5 ID:SGTC_1334|Compound:1486-0610|FD-Score:3.90|P-value:2.23E-4 ID:SGTC_1394|Compound:3474-0001|FD-Score:4.01|P-value:1.50E-4 ID:SGTC_1475|Compound:4092-1048|FD-Score:5.52|P-value:3.51E-7 ID:SGTC_1660|Compound:st012960|FD-Score:4.46|P-value:2.94E-5 ID:SGTC_1672|Compound:st016598|FD-Score:5.90|P-value:5.90E-8 ID:SGTC_1757|Compound:st041620|FD-Score:5.15|P-value:1.87E-6 ID:SGTC_1760|Compound:st045453|FD-Score:3.86|P-value:2.53E-4 ID:SGTC_1781|Compound:st038725|FD-Score:7.29|P-value:3.03E-11 ID:SGTC_1797|Compound:st048695|FD-Score:3.63|P-value:5.35E-4 ID:SGTC_1806|Compound:st050005|FD-Score:3.49|P-value:8.22E-4 ID:SGTC_1874|Compound:staurosporine|FD-Score:3.66|P-value:4.92E-4 ID:SGTC_1944|Compound:st076371|FD-Score:5.63|P-value:2.15E-7 ID:SGTC_1949|Compound:st076024|FD-Score:3.63|P-value:5.35E-4 ID:SGTC_1952|Compound:st077036|FD-Score:4.14|P-value:9.71E-5 ID:SGTC_1954|Compound:st077032|FD-Score:4.12|P-value:1.05E-4 ID:SGTC_1991|Compound:st073000|FD-Score:7.50|P-value:8.38E-12 ID:SGTC_1999|Compound:st074831|FD-Score:3.76|P-value:3.58E-4 ID:SGTC_2006|Compound:4011487|FD-Score:3.55|P-value:6.87E-4 ID:SGTC_2028|Compound:5153890|FD-Score:3.55|P-value:6.91E-4 ID:SGTC_2320|Compound:7373003|FD-Score:3.75|P-value:3.58E-4 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:-14.40|P-value:7.09E-38 ID:SGTC_2699|Compound:st077704|FD-Score:7.26|P-value:3.53E-11 ID:SGTC_2721|Compound:ifenprodil|FD-Score:3.82|P-value:2.91E-4 ID:SGTC_2790|Compound:5423970|FD-Score:4.23|P-value:6.98E-5 ID:SGTC_2792|Compound:7535235|FD-Score:3.78|P-value:3.34E-4 ID:SGTC_2812|Compound:7970281|FD-Score:4.36|P-value:4.31E-5 ID:SGTC_2816|Compound:7982706|FD-Score:3.71|P-value:4.18E-4 ID:SGTC_2887|Compound:9044784|FD-Score:4.76|P-value:9.12E-6 ID:SGTC_2938|Compound:9059746|FD-Score:4.68|P-value:1.29E-5 ID:SGTC_2948|Compound:9070189|FD-Score:4.66|P-value:1.37E-5 ID:SGTC_2965|Compound:9082772|FD-Score:3.64|P-value:5.21E-4 ID:SGTC_3054|Compound:9094397|FD-Score:3.97|P-value:1.73E-4 ID:SGTC_3071|Compound:9117417|FD-Score:4.27|P-value:6.07E-5 ID:SGTC_3081|Compound:9117243|FD-Score:7.63|P-value:3.71E-12 ID:SGTC_3126|Compound:9127922|FD-Score:3.66|P-value:4.79E-4 ID:SGTC_3143|Compound:9086658|FD-Score:-4.05|P-value:1.63E-4 ID:SGTC_3218|Compound:9131285|FD-Score:5.05|P-value:2.81E-6 ID:SGTC_3222|Compound:9128301|FD-Score:-3.68|P-value:5.45E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-4.06|P-value:1.56E-4

Top fitness defect scores for YAL026C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_367 0335-0881 10.30 7.1 µM 8.65E-21 ubiquinone biosynthesis & proteasome 6 2.09E-25
2 SGTC_372 0155-0182 7.92 15.9 µM 6.00E-13 mitochondrial response to ROS 1 1.19E-15
3 SGTC_3081 9117243 7.63 49.5 µM 3.71E-12 fatty acid desaturase (OLE1) 5 1.15E-14
4 SGTC_36 0335-0881 7.54 14.2 µM 6.58E-12 ubiquinone biosynthesis & proteasome 41 2.34E-14
5 SGTC_1991 st073000 7.50 8.9 µM 8.38E-12 NEO1 17 3.16E-14
6 SGTC_954 1189-0853 7.30 2.9 µM 2.92E-11 endomembrane recycling 6 1.49E-13
7 SGTC_1781 st038725 7.29 66.3 µM 3.03E-11 NEO1 37 1.56E-13
8 SGTC_2699 st077704 7.26 54.6 µM 3.53E-11 endomembrane recycling 16 1.88E-13
9 SGTC_1026 k007-1105 6.83 30.6 µM 4.44E-10 ERAD & cell cycle 27 4.36E-12
10 SGTC_637 1181-0519 6.44 7.3 µM 3.74E-9 fatty acid desaturase (OLE1) 48 6.13E-11
11 SGTC_1672 st016598 5.90 6.5 µM 5.90E-8 endomembrane recycling 32 1.87E-9
12 SGTC_1160 4092-0570 5.76 10.5 µM 1.11E-7 plasma membrane duress 17 4.09E-9
13 SGTC_172 1112-0046 5.65 2.6 µM 1.94E-7 NEO1 14 8.12E-9
14 SGTC_1944 st076371 5.63 56.9 µM 2.15E-7 6 9.26E-9
15 SGTC_1475 4092-1048 5.52 11.7 µM 3.51E-7 plasma membrane duress 11 1.70E-8
16 SGTC_181 1447-1644 5.44 8.1 µM 5.09E-7 endomembrane recycling 18 2.68E-8
17 SGTC_1757 st041620 5.15 31.8 µM 1.87E-6 18 1.34E-7
18 SGTC_950 1165-0508 5.07 94.1 µM 2.55E-6 11 1.97E-7
19 SGTC_258 haloperidol 5.06 50.8 µM 2.64E-6 fatty acid desaturase (OLE1) 15 2.05E-7
20 SGTC_3218 9131285 5.05 49.5 µM 2.81E-6 endomembrane recycling 15 2.22E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.446 1.41E-163 YCR094W CDC50 hom Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
0.429 4.83E-150 YJL204C RCY1 hom F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
0.323 2.68E-82 YER031C YPT31 hom Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication
0.320 5.89E-81 YDL226C GCS1 hom ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
0.258 3.56E-52 YGL019W CKB1 hom Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
0.256 2.89E-51 YJL207C LAA1 hom AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene
0.226 3.85E-40 YOR039W CKB2 hom Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
0.212 2.38E-35 YOR070C GYP1 hom Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
0.196 1.61E-30 YJR060W CBF1 hom Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress
0.192 4.25E-29 YGL055W OLE1 het Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
0.183 8.94E-27 YIL048W NEO1 het Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
0.179 1.28E-25 YJL193W_p YJL193W_p hom Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect
0.170 6.04E-23 YDL090C RAM1 hom Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit
0.169 7.70E-23 YML034C-A_d YML034C-A_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.169 8.10E-23 YFL034W_p YFL034W_p hom Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk