YAL034W-A / MTW1

Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly

Zygosity: Heterozygous strain
fixedexpanded
Profile for YAL034W-A / MTW1

Click on Significant Values for Screen Details ID:SGTC_33|Compound:0986-0033|FD-Score:3.22|P-value:1.65E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.02|P-value:3.61E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-6.28|P-value:1.14E-12 ID:SGTC_143|Compound:4032-1194|FD-Score:-3.27|P-value:1.29E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:-4.35|P-value:5.67E-7 ID:SGTC_147|Compound:4466-0538|FD-Score:4.58|P-value:1.68E-7 ID:SGTC_153|Compound:4077-0274|FD-Score:4.33|P-value:6.88E-7 ID:SGTC_155|Compound:k072-0202|FD-Score:2.87|P-value:6.87E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:-2.91|P-value:5.75E-4 ID:SGTC_384|Compound:k007-0144|FD-Score:2.84|P-value:7.79E-4 ID:SGTC_396|Compound:dithiazanine|FD-Score:-3.04|P-value:3.30E-4 ID:SGTC_398|Compound:bronopol|FD-Score:3.80|P-value:1.14E-5 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:2.93|P-value:5.63E-4 ID:SGTC_408|Compound:cantharidin|FD-Score:6.53|P-value:1.66E-13 ID:SGTC_412|Compound:gemfibrozil|FD-Score:2.97|P-value:4.76E-4 ID:SGTC_985|Compound:1493-0307|FD-Score:3.56|P-value:3.70E-5 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:-2.86|P-value:6.90E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-5.27|P-value:1.89E-9 ID:SGTC_1287|Compound:0971-0001|FD-Score:2.86|P-value:7.37E-4 ID:SGTC_1384|Compound:3089-0840|FD-Score:2.85|P-value:7.52E-4 ID:SGTC_1389|Compound:3405-0187|FD-Score:-3.09|P-value:2.71E-4 ID:SGTC_1394|Compound:3474-0001|FD-Score:3.83|P-value:1.00E-5 ID:SGTC_1524|Compound:corynanthine|FD-Score:8.45|P-value:2.28E-21 ID:SGTC_1526|Compound:st051518|FD-Score:-5.83|P-value:3.68E-11 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:2.92|P-value:5.79E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.25|P-value:1.48E-4 ID:SGTC_1701|Compound:st028638|FD-Score:-3.34|P-value:9.16E-5 ID:SGTC_1901|Compound:st060216|FD-Score:3.00|P-value:4.27E-4 ID:SGTC_1903|Compound:st060211|FD-Score:3.11|P-value:2.69E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:-3.62|P-value:2.58E-5 ID:SGTC_2533|Compound:agelasine|FD-Score:3.70|P-value:1.92E-5 ID:SGTC_2583|Compound:asiatic acid|FD-Score:4.26|P-value:1.04E-6 ID:SGTC_2692|Compound:st077426|FD-Score:3.52|P-value:4.40E-5 ID:SGTC_2785|Compound:7756463|FD-Score:-3.11|P-value:2.54E-4 ID:SGTC_2796|Compound:7949634|FD-Score:-2.89|P-value:6.08E-4 ID:SGTC_2801|Compound:7907800|FD-Score:-3.15|P-value:2.11E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-3.23|P-value:1.49E-4 ID:SGTC_3064|Compound:1327-0027|FD-Score:4.03|P-value:3.61E-6 ID:SGTC_3189|Compound:9110682|FD-Score:3.22|P-value:1.67E-4 ID:SGTC_33|Compound:0986-0033|FD-Score:3.22|P-value:1.65E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.02|P-value:3.61E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-6.28|P-value:1.14E-12 ID:SGTC_143|Compound:4032-1194|FD-Score:-3.27|P-value:1.29E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:-4.35|P-value:5.67E-7 ID:SGTC_147|Compound:4466-0538|FD-Score:4.58|P-value:1.68E-7 ID:SGTC_153|Compound:4077-0274|FD-Score:4.33|P-value:6.88E-7 ID:SGTC_155|Compound:k072-0202|FD-Score:2.87|P-value:6.87E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:-2.91|P-value:5.75E-4 ID:SGTC_384|Compound:k007-0144|FD-Score:2.84|P-value:7.79E-4 ID:SGTC_396|Compound:dithiazanine|FD-Score:-3.04|P-value:3.30E-4 ID:SGTC_398|Compound:bronopol|FD-Score:3.80|P-value:1.14E-5 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:2.93|P-value:5.63E-4 ID:SGTC_408|Compound:cantharidin|FD-Score:6.53|P-value:1.66E-13 ID:SGTC_412|Compound:gemfibrozil|FD-Score:2.97|P-value:4.76E-4 ID:SGTC_985|Compound:1493-0307|FD-Score:3.56|P-value:3.70E-5 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:-2.86|P-value:6.90E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-5.27|P-value:1.89E-9 ID:SGTC_1287|Compound:0971-0001|FD-Score:2.86|P-value:7.37E-4 ID:SGTC_1384|Compound:3089-0840|FD-Score:2.85|P-value:7.52E-4 ID:SGTC_1389|Compound:3405-0187|FD-Score:-3.09|P-value:2.71E-4 ID:SGTC_1394|Compound:3474-0001|FD-Score:3.83|P-value:1.00E-5 ID:SGTC_1524|Compound:corynanthine|FD-Score:8.45|P-value:2.28E-21 ID:SGTC_1526|Compound:st051518|FD-Score:-5.83|P-value:3.68E-11 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:2.92|P-value:5.79E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.25|P-value:1.48E-4 ID:SGTC_1701|Compound:st028638|FD-Score:-3.34|P-value:9.16E-5 ID:SGTC_1901|Compound:st060216|FD-Score:3.00|P-value:4.27E-4 ID:SGTC_1903|Compound:st060211|FD-Score:3.11|P-value:2.69E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:-3.62|P-value:2.58E-5 ID:SGTC_2533|Compound:agelasine|FD-Score:3.70|P-value:1.92E-5 ID:SGTC_2583|Compound:asiatic acid|FD-Score:4.26|P-value:1.04E-6 ID:SGTC_2692|Compound:st077426|FD-Score:3.52|P-value:4.40E-5 ID:SGTC_2785|Compound:7756463|FD-Score:-3.11|P-value:2.54E-4 ID:SGTC_2796|Compound:7949634|FD-Score:-2.89|P-value:6.08E-4 ID:SGTC_2801|Compound:7907800|FD-Score:-3.15|P-value:2.11E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-3.23|P-value:1.49E-4 ID:SGTC_3064|Compound:1327-0027|FD-Score:4.03|P-value:3.61E-6 ID:SGTC_3189|Compound:9110682|FD-Score:3.22|P-value:1.67E-4

Top fitness defect scores for YAL034W-A deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1524 corynanthine 8.45 62.1 µM 2.28E-21 1 1.50E-17 3.35
2 SGTC_408 cantharidin 6.53 100.0 µM 1.66E-13 cell wall signaling 3 3.27E-11 0.10
3 SGTC_147 4466-0538 4.58 29.2 µM 1.68E-7 ergosterol biosynthesis 3 2.34E-6 0.09
4 SGTC_153 4077-0274 4.33 519.7 µM 6.88E-7 5 7.34E-6 0.36
5 SGTC_2583 asiatic acid 4.26 100.0 µM 1.04E-6 6 1.02E-5 0.09
6 SGTC_3064 1327-0027 4.03 10.0 µM 3.61E-6 5 2.81E-5 0.19
7 SGTC_1394 3474-0001 3.83 65.7 µM 1.00E-5 endomembrane recycling 5 6.45E-5 0.28
8 SGTC_398 bronopol 3.80 33.3 µM 1.14E-5 RNA processing & uracil transport 11 7.16E-5 0.04
9 SGTC_2533 agelasine 3.70 13.7 µM 1.92E-5 ERG2 16 1.10E-4 0.05
10 SGTC_985 1493-0307 3.56 8.7 µM 3.70E-5 TSC3-RPN4 4 1.87E-4 0.02
11 SGTC_2692 st077426 3.52 47.7 µM 4.40E-5 9 2.15E-4 0.07
12 SGTC_1611 st001856 3.25 99.4 µM 1.48E-4 20 5.77E-4 0.08
13 SGTC_33 0986-0033 3.22 35.8 µM 1.65E-4 calcium & mitochondrial duress 5 6.30E-4 0.05
14 SGTC_3189 9110682 3.22 49.5 µM 1.67E-4 14 6.39E-4 0.01
15 SGTC_1903 st060211 3.11 78.3 µM 2.69E-4 7 9.42E-4 0.12
16 SGTC_1901 st060216 3.00 58.6 µM 4.27E-4 10 0.00137 0.04
17 SGTC_412 gemfibrozil 2.97 100.0 µM 4.76E-4 DNA intercalators 21 0.00150 0.07
18 SGTC_402 epigallocatechin gallate 2.93 10.0 µM 5.63E-4 20 0.00172 0.13
19 SGTC_1576 erythromycin ethylsuccinate 2.92 23.2 µM 5.79E-4 13 0.00176 0.02
20 SGTC_155 k072-0202 2.87 43.6 µM 6.87E-4 18 0.00202 0.06

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.179 1.30E-25 YFR027W ECO1 het Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress
0.178 2.80E-25 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.173 5.86E-24 YML098W TAF13 het TFIID subunit (19 kDa), involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors
0.167 1.67E-22 YDR088C SLU7 het RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
0.162 3.32E-21 YBR276C PPS1 hom Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle
0.159 1.63E-20 YAL003W EFB1 het Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site
0.157 6.81E-20 YFR031C SMC2 het Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus
0.149 3.88E-18 YDR044W HEM13 het Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p)
0.148 7.23E-18 YLR353W BUD8 hom Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole
0.147 1.34E-17 YDL060W TSR1 het Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.146 2.33E-17 YDL174C DLD1 hom D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane
0.145 2.49E-17 YOR020W-A_p YOR020W-A_p hom Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.137 1.69E-15 YMR129W POM152 hom Glycoprotein subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NUP210
0.130 3.86E-14 YLR117C CLF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
0.130 4.65E-14 YER013W PRP22 het DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes