HIPHOP chemogenomics database

YAL043C / PTA1

Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII

Zygosity: Heterozygous strain

fixedexpanded
Profile for YAL043C / PTA1

Click on Significant Values for Screen Details

Top fitness defect scores for YAL043C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_773	4358-1457	8.05	44.6 µM	1.95E-21		4	3.99E-16	0.88
2	SGTC_767	0929-0023	4.51	86.0 µM	6.78E-8	TRP & mitochondrial translation	9	3.24E-6	0.21
3	SGTC_163	1327-0036	3.82	7.5 µM	4.16E-6		48	6.76E-5	0.09
4	SGTC_122	0236-0016	3.74	402.4 µM	6.40E-6	RNA processing & uracil transport	17	9.31E-5	0.02
5	SGTC_234	nsc-17383	3.34	10.7 µM	4.92E-5	ERG2	9	4.22E-4	0.02
6	SGTC_3336	9143619	3.16	71.4 µM	1.12E-4		2	7.80E-4	0.22
7	SGTC_18	0111-0177	3.07	500.0 nM	1.72E-4		12	0.00107	0.00
8	SGTC_2708	st078559	2.95	56.0 µM	2.88E-4		6	0.00157	0.01
9	SGTC_1319	1306-0155	2.89	43.7 µM	3.84E-4		17	0.00195	0.05
10	SGTC_826	1683-5028	2.88	118.0 µM	3.89E-4		11	0.00197	0.06
11	SGTC_3344	9148862	2.85	3.9 µM	4.57E-4		13	0.00222	0.01
12	SGTC_1892	st058411	2.77	7.4 µM	6.19E-4		18	0.00279	0.03
13	SGTC_388	3',4'-dichloroisocoumarin	2.75	16.7 µM	6.78E-4		21	0.00298	0.01
14	SGTC_1846	st056244	2.73	14.8 µM	7.28E-4		19	0.00314	0.11
15	SGTC_2555	cholic acid	2.70	100.0 µM	8.35E-4		8	0.00348	0.06
16	SGTC_457	grayanotoxin iii	2.68	135.0 µM	8.85E-4		38	0.00364	0.02
17	SGTC_1026	k007-1105	2.64	30.6 µM	0.00104	ERAD & cell cycle	42	0.00411	0.03
18	SGTC_902	1120-0002	2.64	2.1 µM	0.00104	azole & statin	34	0.00411	0.02
19	SGTC_1861	st057647	2.64	17.4 µM	0.00106		17	0.00417	0.02
20	SGTC_632	0781-2353	2.61	4.7 µM	0.00119	Golgi	42	0.00454	0.03

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.213	9.55E-36	YLR053C_p	YLR053C_p	hom	Putative protein of unknown function
0.196	4.20E-30	YCL026C-A	FRM2	hom	Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin
0.194	7.56E-30	YLR212C	TUB4	het	Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress
0.194	1.30E-29	YDR055W	PST1	hom	Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1
0.188	5.27E-28	YFL024C	EPL1	het	Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb
0.170	4.52E-23	YIR035C_p	YIR035C_p	hom	Putative cytoplasmic short-chain dehydrogenase/reductase
0.158	3.16E-20	YKR054C	DYN1	hom	Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p
0.146	1.52E-17	YER052C	HOM3	hom	Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.145	4.12E-17	YGL220W	FRA2	hom	Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel
0.145	3.18E-17	YOR238W_p	YOR238W_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.144	4.68E-17	YGR193C	PDX1	hom	Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core
0.142	1.33E-16	YDL104C	QRI7	hom	Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification
0.139	5.16E-16	YIL003W	CFD1	het	Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol
0.139	6.27E-16	YJR001W	AVT1	hom	Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
0.138	1.05E-15	YDR166C	SEC5	het	Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip