YAL048C / GEM1

Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases

Zygosity: Homozygous strain
fixedexpanded
Profile for YAL048C / GEM1

Click on Significant Values for Screen Details ID:SGTC_101|Compound:0490-5701|FD-Score:4.99|P-value:9.55E-8 ID:SGTC_279|Compound:1273-0055|FD-Score:3.10|P-value:5.80E-4 ID:SGTC_288|Compound:1315-0019|FD-Score:-3.04|P-value:9.43E-4 ID:SGTC_320|Compound:4092-0846|FD-Score:3.45|P-value:1.53E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:3.05|P-value:6.77E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:4.78|P-value:3.01E-7 ID:SGTC_529|Compound:4076-0302|FD-Score:3.24|P-value:3.34E-4 ID:SGTC_617|Compound:3770-0098|FD-Score:3.02|P-value:7.72E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.71|P-value:7.20E-5 ID:SGTC_753|Compound:1322-0200|FD-Score:-3.03|P-value:9.64E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:3.15|P-value:4.71E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:6.77|P-value:8.91E-13 ID:SGTC_935|Compound:2996-0153|FD-Score:3.79|P-value:3.68E-5 ID:SGTC_1039|Compound:k821-0043|FD-Score:3.58|P-value:8.86E-5 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.12|P-value:5.29E-4 ID:SGTC_1103|Compound:duloxetine|FD-Score:2.99|P-value:8.54E-4 ID:SGTC_1120|Compound:ficusin|FD-Score:-3.78|P-value:5.34E-5 ID:SGTC_1121|Compound:fusaric acid|FD-Score:-3.41|P-value:2.41E-4 ID:SGTC_1241|Compound:0364-0022|FD-Score:3.49|P-value:1.29E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:3.57|P-value:9.34E-5 ID:SGTC_1341|Compound:1486-1360|FD-Score:4.60|P-value:7.76E-7 ID:SGTC_1555|Compound:citronellol|FD-Score:3.79|P-value:3.68E-5 ID:SGTC_1696|Compound:st025591|FD-Score:5.22|P-value:2.55E-8 ID:SGTC_1750|Compound:st081859|FD-Score:5.68|P-value:1.57E-9 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:4.89|P-value:1.65E-7 ID:SGTC_1787|Compound:fluconazole|FD-Score:-4.35|P-value:3.92E-6 ID:SGTC_1792|Compound:st045773|FD-Score:8.73|P-value:5.07E-20 ID:SGTC_1912|Compound:st060209|FD-Score:3.54|P-value:1.02E-4 ID:SGTC_1964|Compound:st076914|FD-Score:2.95|P-value:9.91E-4 ID:SGTC_2098|Compound:5376439|FD-Score:-3.37|P-value:2.73E-4 ID:SGTC_2382|Compound:9035899|FD-Score:7.37|P-value:7.96E-15 ID:SGTC_2401|Compound:5128683|FD-Score:4.05|P-value:1.14E-5 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:-4.26|P-value:6.06E-6 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.68|P-value:5.83E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.00|P-value:8.31E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:5.54|P-value:3.64E-9 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.56|P-value:9.57E-5 ID:SGTC_2766|Compound:oltipraz|FD-Score:3.55|P-value:9.89E-5 ID:SGTC_2832|Compound:7992762|FD-Score:-7.42|P-value:1.04E-14 ID:SGTC_2960|Compound:9079389|FD-Score:4.69|P-value:4.91E-7 ID:SGTC_2971|Compound:9092186|FD-Score:-3.19|P-value:5.47E-4 ID:SGTC_3011|Compound:9081274|FD-Score:4.25|P-value:4.43E-6 ID:SGTC_3019|Compound:9081971|FD-Score:-3.11|P-value:7.42E-4 ID:SGTC_3024|Compound:9090631|FD-Score:2.95|P-value:9.71E-4 ID:SGTC_3032|Compound:9089427|FD-Score:4.11|P-value:8.62E-6 ID:SGTC_3240|Compound:9133527|FD-Score:3.05|P-value:6.92E-4 ID:SGTC_101|Compound:0490-5701|FD-Score:4.99|P-value:9.55E-8 ID:SGTC_279|Compound:1273-0055|FD-Score:3.10|P-value:5.80E-4 ID:SGTC_288|Compound:1315-0019|FD-Score:-3.04|P-value:9.43E-4 ID:SGTC_320|Compound:4092-0846|FD-Score:3.45|P-value:1.53E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:3.05|P-value:6.77E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:4.78|P-value:3.01E-7 ID:SGTC_529|Compound:4076-0302|FD-Score:3.24|P-value:3.34E-4 ID:SGTC_617|Compound:3770-0098|FD-Score:3.02|P-value:7.72E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.71|P-value:7.20E-5 ID:SGTC_753|Compound:1322-0200|FD-Score:-3.03|P-value:9.64E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:3.15|P-value:4.71E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:6.77|P-value:8.91E-13 ID:SGTC_935|Compound:2996-0153|FD-Score:3.79|P-value:3.68E-5 ID:SGTC_1039|Compound:k821-0043|FD-Score:3.58|P-value:8.86E-5 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.12|P-value:5.29E-4 ID:SGTC_1103|Compound:duloxetine|FD-Score:2.99|P-value:8.54E-4 ID:SGTC_1120|Compound:ficusin|FD-Score:-3.78|P-value:5.34E-5 ID:SGTC_1121|Compound:fusaric acid|FD-Score:-3.41|P-value:2.41E-4 ID:SGTC_1241|Compound:0364-0022|FD-Score:3.49|P-value:1.29E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:3.57|P-value:9.34E-5 ID:SGTC_1341|Compound:1486-1360|FD-Score:4.60|P-value:7.76E-7 ID:SGTC_1555|Compound:citronellol|FD-Score:3.79|P-value:3.68E-5 ID:SGTC_1696|Compound:st025591|FD-Score:5.22|P-value:2.55E-8 ID:SGTC_1750|Compound:st081859|FD-Score:5.68|P-value:1.57E-9 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:4.89|P-value:1.65E-7 ID:SGTC_1787|Compound:fluconazole|FD-Score:-4.35|P-value:3.92E-6 ID:SGTC_1792|Compound:st045773|FD-Score:8.73|P-value:5.07E-20 ID:SGTC_1912|Compound:st060209|FD-Score:3.54|P-value:1.02E-4 ID:SGTC_1964|Compound:st076914|FD-Score:2.95|P-value:9.91E-4 ID:SGTC_2098|Compound:5376439|FD-Score:-3.37|P-value:2.73E-4 ID:SGTC_2382|Compound:9035899|FD-Score:7.37|P-value:7.96E-15 ID:SGTC_2401|Compound:5128683|FD-Score:4.05|P-value:1.14E-5 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:-4.26|P-value:6.06E-6 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.68|P-value:5.83E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.00|P-value:8.31E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:5.54|P-value:3.64E-9 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.56|P-value:9.57E-5 ID:SGTC_2766|Compound:oltipraz|FD-Score:3.55|P-value:9.89E-5 ID:SGTC_2832|Compound:7992762|FD-Score:-7.42|P-value:1.04E-14 ID:SGTC_2960|Compound:9079389|FD-Score:4.69|P-value:4.91E-7 ID:SGTC_2971|Compound:9092186|FD-Score:-3.19|P-value:5.47E-4 ID:SGTC_3011|Compound:9081274|FD-Score:4.25|P-value:4.43E-6 ID:SGTC_3019|Compound:9081971|FD-Score:-3.11|P-value:7.42E-4 ID:SGTC_3024|Compound:9090631|FD-Score:2.95|P-value:9.71E-4 ID:SGTC_3032|Compound:9089427|FD-Score:4.11|P-value:8.62E-6 ID:SGTC_3240|Compound:9133527|FD-Score:3.05|P-value:6.92E-4

Top fitness defect scores for YAL048C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1792 st045773 8.73 43.6 µM 5.07E-20 1 1.26E-18
2 SGTC_2382 9035899 7.37 200.0 µM 7.96E-15 60S ribosome export 1 8.34E-14
3 SGTC_852 0418-0143 6.77 439.0 µM 8.91E-13 calcium & mitochondrial duress 18 6.64E-12
4 SGTC_1750 st081859 5.68 2.4 µM 1.57E-9 endomembrane recycling 115 6.81E-9
5 SGTC_2754 granisetron 5.54 64.0 µM 3.64E-9 11 1.48E-8
6 SGTC_1696 st025591 5.22 34.8 µM 2.55E-8 SWF1 & branched chain AA biosynthesis 12 8.98E-8
7 SGTC_101 0490-5701 4.99 117.5 µM 9.55E-8 3 3.06E-7
8 SGTC_1785 bathophenanthroline 4.89 24.0 µM 1.65E-7 heme requiring 20 5.09E-7
9 SGTC_524 r073-0010 4.78 27.8 µM 3.01E-7 32 8.86E-7
10 SGTC_2960 9079389 4.69 6.0 µM 4.91E-7 13 1.40E-6
11 SGTC_1341 1486-1360 4.60 280.0 µM 7.76E-7 10 2.13E-6
12 SGTC_3011 9081274 4.25 71.4 µM 4.43E-6 9 1.07E-5
13 SGTC_3032 9089427 4.11 49.5 µM 8.62E-6 60S ribosome export 12 1.99E-5
14 SGTC_2401 5128683 4.05 200.0 µM 1.14E-5 34 2.58E-5
15 SGTC_1555 citronellol 3.79 128.0 µM 3.68E-5 3 7.61E-5
16 SGTC_935 2996-0153 3.79 430.0 µM 3.68E-5 PDR1 23 7.61E-5
17 SGTC_2520 obtusaquinone 3.68 4.7 µM 5.83E-5 26 1.17E-4
18 SGTC_1039 k821-0043 3.58 94.0 µM 8.86E-5 Golgi 42 1.72E-4
19 SGTC_1298 1079-1278 3.57 263.0 µM 9.34E-5 DNA damage response 30 1.81E-4
20 SGTC_2757 clomiphene 3.56 4.2 µM 9.57E-5 fatty acid desaturase (OLE1) 54 1.85E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.162 4.50E-21 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.133 1.14E-14 YPR029C APL4 hom Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
0.132 1.51E-14 YPR153W YPR153W hom Putative protein of unknown function
0.124 4.68E-13 YJR087W_d YJR087W_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and ECM2
0.123 8.62E-13 YCL046W_d YCL046W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C
0.115 2.90E-11 YOL115W PAP2 hom Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p
0.114 2.99E-11 YJL029C VPS53 hom Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting
0.114 4.47E-11 YKL098W MTC2 hom Protein of unknown function; mtc2 is synthetically sick with cdc13-1
0.112 7.78E-11 YER171W RAD3 het 5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress
0.107 5.54E-10 YMR007W_d YMR007W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.103 1.85E-9 YOR202W HIS3 hom Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p
0.101 4.07E-9 YCR031C RPS14A hom Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication
0.098 1.15E-8 YOR120W GCY1 hom Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication
0.098 1.44E-8 YGR155W CYS4 hom Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria
0.097 1.64E-8 YBR255W MTC4 hom Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1