YBL002W / HTB2

Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation

Zygosity: Homozygous strain
fixedexpanded
Profile for YBL002W / HTB2

Click on Significant Values for Screen Details ID:SGTC_333|Compound:3486-0131|FD-Score:-2.91|P-value:5.13E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:-3.65|P-value:1.87E-5 ID:SGTC_347|Compound:4534-1311|FD-Score:-3.61|P-value:2.24E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:2.81|P-value:8.00E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:4.33|P-value:5.71E-7 ID:SGTC_508|Compound:roscovitine|FD-Score:4.47|P-value:2.51E-7 ID:SGTC_509|Compound:pifithrin|FD-Score:3.00|P-value:3.82E-4 ID:SGTC_521|Compound:r062-0005|FD-Score:2.89|P-value:5.91E-4 ID:SGTC_540|Compound:1187-0821|FD-Score:-3.19|P-value:1.57E-4 ID:SGTC_760|Compound:0568-0725|FD-Score:4.60|P-value:1.19E-7 ID:SGTC_767|Compound:0929-0023|FD-Score:-4.04|P-value:2.60E-6 ID:SGTC_1023|Compound:4482-3888|FD-Score:3.10|P-value:2.46E-4 ID:SGTC_1084|Compound:voriconazole|FD-Score:3.08|P-value:2.69E-4 ID:SGTC_1297|Compound:1068-0111|FD-Score:3.42|P-value:6.01E-5 ID:SGTC_1412|Compound:3909-8563|FD-Score:2.76|P-value:9.89E-4 ID:SGTC_1563|Compound:conessine|FD-Score:3.62|P-value:2.42E-5 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:3.92|P-value:5.38E-6 ID:SGTC_1569|Compound:carminic acid|FD-Score:3.77|P-value:1.15E-5 ID:SGTC_1886|Compound:st057356|FD-Score:-3.80|P-value:8.90E-6 ID:SGTC_1903|Compound:st060211|FD-Score:3.25|P-value:1.33E-4 ID:SGTC_1905|Compound:st060207|FD-Score:2.86|P-value:6.52E-4 ID:SGTC_1982|Compound:st072467|FD-Score:-2.80|P-value:7.69E-4 ID:SGTC_2248|Compound:tofa|FD-Score:-2.79|P-value:7.93E-4 ID:SGTC_2252|Compound:omeprazole|FD-Score:-4.28|P-value:6.94E-7 ID:SGTC_2419|Compound:paclitaxel|FD-Score:2.99|P-value:3.86E-4 ID:SGTC_2429|Compound:5902844|FD-Score:3.52|P-value:3.84E-5 ID:SGTC_2514|Compound:salinomycin|FD-Score:3.46|P-value:5.18E-5 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-3.69|P-value:1.51E-5 ID:SGTC_2537|Compound:larixol|FD-Score:3.04|P-value:3.22E-4 ID:SGTC_2830|Compound:7992007|FD-Score:-4.38|P-value:3.86E-7 ID:SGTC_2832|Compound:7992762|FD-Score:-3.20|P-value:1.48E-4 ID:SGTC_2967|Compound:9089538|FD-Score:4.59|P-value:1.20E-7 ID:SGTC_3048|Compound:9093155|FD-Score:3.66|P-value:1.98E-5 ID:SGTC_3063|Compound:1327-0034|FD-Score:3.59|P-value:2.69E-5 ID:SGTC_3165|Compound:9101544|FD-Score:3.24|P-value:1.38E-4 ID:SGTC_3179|Compound:9107801|FD-Score:-3.02|P-value:3.28E-4 ID:SGTC_333|Compound:3486-0131|FD-Score:-2.91|P-value:5.13E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:-3.65|P-value:1.87E-5 ID:SGTC_347|Compound:4534-1311|FD-Score:-3.61|P-value:2.24E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:2.81|P-value:8.00E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:4.33|P-value:5.71E-7 ID:SGTC_508|Compound:roscovitine|FD-Score:4.47|P-value:2.51E-7 ID:SGTC_509|Compound:pifithrin|FD-Score:3.00|P-value:3.82E-4 ID:SGTC_521|Compound:r062-0005|FD-Score:2.89|P-value:5.91E-4 ID:SGTC_540|Compound:1187-0821|FD-Score:-3.19|P-value:1.57E-4 ID:SGTC_760|Compound:0568-0725|FD-Score:4.60|P-value:1.19E-7 ID:SGTC_767|Compound:0929-0023|FD-Score:-4.04|P-value:2.60E-6 ID:SGTC_1023|Compound:4482-3888|FD-Score:3.10|P-value:2.46E-4 ID:SGTC_1084|Compound:voriconazole|FD-Score:3.08|P-value:2.69E-4 ID:SGTC_1297|Compound:1068-0111|FD-Score:3.42|P-value:6.01E-5 ID:SGTC_1412|Compound:3909-8563|FD-Score:2.76|P-value:9.89E-4 ID:SGTC_1563|Compound:conessine|FD-Score:3.62|P-value:2.42E-5 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:3.92|P-value:5.38E-6 ID:SGTC_1569|Compound:carminic acid|FD-Score:3.77|P-value:1.15E-5 ID:SGTC_1886|Compound:st057356|FD-Score:-3.80|P-value:8.90E-6 ID:SGTC_1903|Compound:st060211|FD-Score:3.25|P-value:1.33E-4 ID:SGTC_1905|Compound:st060207|FD-Score:2.86|P-value:6.52E-4 ID:SGTC_1982|Compound:st072467|FD-Score:-2.80|P-value:7.69E-4 ID:SGTC_2248|Compound:tofa|FD-Score:-2.79|P-value:7.93E-4 ID:SGTC_2252|Compound:omeprazole|FD-Score:-4.28|P-value:6.94E-7 ID:SGTC_2419|Compound:paclitaxel|FD-Score:2.99|P-value:3.86E-4 ID:SGTC_2429|Compound:5902844|FD-Score:3.52|P-value:3.84E-5 ID:SGTC_2514|Compound:salinomycin|FD-Score:3.46|P-value:5.18E-5 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-3.69|P-value:1.51E-5 ID:SGTC_2537|Compound:larixol|FD-Score:3.04|P-value:3.22E-4 ID:SGTC_2830|Compound:7992007|FD-Score:-4.38|P-value:3.86E-7 ID:SGTC_2832|Compound:7992762|FD-Score:-3.20|P-value:1.48E-4 ID:SGTC_2967|Compound:9089538|FD-Score:4.59|P-value:1.20E-7 ID:SGTC_3048|Compound:9093155|FD-Score:3.66|P-value:1.98E-5 ID:SGTC_3063|Compound:1327-0034|FD-Score:3.59|P-value:2.69E-5 ID:SGTC_3165|Compound:9101544|FD-Score:3.24|P-value:1.38E-4 ID:SGTC_3179|Compound:9107801|FD-Score:-3.02|P-value:3.28E-4

Top fitness defect scores for YBL002W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_760 0568-0725 4.60 82.9 µM 1.19E-7 4 2.16E-6
2 SGTC_2967 9089538 4.59 49.9 µM 1.20E-7 16 2.16E-6
3 SGTC_508 roscovitine 4.47 141.0 µM 2.51E-7 1 3.90E-6
4 SGTC_358 0075-0058 4.33 9.4 µM 5.71E-7 48 7.51E-6
5 SGTC_1566 6,7-dimethoxy-4-ethylcoumarin 3.92 85.4 µM 5.38E-6 22 4.50E-5
6 SGTC_1569 carminic acid 3.77 40.6 µM 1.15E-5 19 8.25E-5
7 SGTC_3048 9093155 3.66 49.5 µM 1.98E-5 8 1.27E-4
8 SGTC_1563 conessine 3.62 56.1 µM 2.42E-5 24 1.49E-4
9 SGTC_3063 1327-0034 3.59 4.0 µM 2.69E-5 53 1.63E-4
10 SGTC_2429 5902844 3.52 64.4 µM 3.84E-5 46 2.16E-4
11 SGTC_2514 salinomycin 3.46 42.7 µM 5.18E-5 74 2.75E-4
12 SGTC_1297 1068-0111 3.42 14.1 µM 6.01E-5 25 3.10E-4
13 SGTC_1903 st060211 3.25 78.3 µM 1.33E-4 55 5.84E-4
14 SGTC_3165 9101544 3.24 49.5 µM 1.38E-4 26 6.01E-4
15 SGTC_1023 4482-3888 3.10 16.0 µM 2.46E-4 TSC3-RPN4 51 9.60E-4
16 SGTC_1084 voriconazole 3.08 435.0 nM 2.69E-4 azole & statin 58 0.00103
17 SGTC_2537 larixol 3.04 68.9 µM 3.22E-4 71 0.00119
18 SGTC_509 pifithrin 3.00 175.0 µM 3.82E-4 mitochondrial processes 26 0.00137
19 SGTC_2419 paclitaxel 2.99 166.5 µM 3.86E-4 108 0.00138
20 SGTC_521 r062-0005 2.89 13.8 µM 5.91E-4 59 0.00194

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.152 6.97E-19 YGR201C_p YGR201C_p hom Putative protein of unknown function
0.151 1.61E-18 YNL104C LEU4 hom Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication
0.144 5.08E-17 YDR042C_p YDR042C_p hom Putative protein of unknown function; expression is increased in ssu72-ts69 mutant
0.132 1.85E-14 YML108W YML108W hom Protein of unknown function; structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress
0.131 2.13E-14 YKL030W_d YKL030W_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene MAE1
0.126 2.44E-13 YDR306C_p YDR306C_p hom F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain
0.120 3.13E-12 YDR277C MTH1 hom Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation
0.117 1.04E-11 YMR140W SIP5 hom Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress
0.116 1.44E-11 YBR205W KTR3 hom Putative alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles; relocalizes from nucleus to vacuole upon DNA replication stress
0.113 4.99E-11 YDR420W HKR1 hom Mucin family member that functions as an osmosensor in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection
0.113 5.16E-11 YGR096W TPC1 hom Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family
0.108 3.95E-10 YLR154C RNH203 hom Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS3 that causes Aicardi-Goutieres syndrome
0.106 6.55E-10 YLR394W CST9 hom SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate
0.105 1.21E-9 YLR237W THI7 hom Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia
0.101 3.96E-9 YLR287C_p YLR287C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene