YBL032W / HEK2

RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K

Zygosity: Homozygous strain
fixedexpanded
Profile for YBL032W / HEK2

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:-3.29|P-value:3.19E-4 ID:SGTC_112|Compound:phenothiazine|FD-Score:-3.03|P-value:8.29E-4 ID:SGTC_113|Compound:0099-0221|FD-Score:-3.44|P-value:1.75E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:10.80|P-value:1.70E-29 ID:SGTC_424|Compound:splitomicin|FD-Score:4.15|P-value:8.30E-6 ID:SGTC_513|Compound:4245-1575|FD-Score:-3.50|P-value:1.39E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:4.05|P-value:1.31E-5 ID:SGTC_541|Compound:4513-1321|FD-Score:3.08|P-value:7.07E-4 ID:SGTC_722|Compound:3534-0461|FD-Score:3.31|P-value:2.98E-4 ID:SGTC_752|Compound:1896-0090|FD-Score:4.02|P-value:1.52E-5 ID:SGTC_821|Compound:1114-0266|FD-Score:3.24|P-value:3.95E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:4.12|P-value:9.79E-6 ID:SGTC_914|Compound:3820-6779|FD-Score:4.41|P-value:2.45E-6 ID:SGTC_1103|Compound:duloxetine|FD-Score:4.11|P-value:1.02E-5 ID:SGTC_1105|Compound:epirubicin|FD-Score:3.32|P-value:2.92E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.55|P-value:1.16E-4 ID:SGTC_1234|Compound:0326-0494|FD-Score:3.84|P-value:3.36E-5 ID:SGTC_1311|Compound:1190-0148|FD-Score:3.22|P-value:4.21E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:3.07|P-value:7.35E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:-2.98|P-value:9.90E-4 ID:SGTC_1570|Compound:verbenone|FD-Score:3.77|P-value:4.56E-5 ID:SGTC_1759|Compound:st043059|FD-Score:4.19|P-value:6.98E-6 ID:SGTC_1849|Compound:st056239|FD-Score:-3.22|P-value:4.15E-4 ID:SGTC_1894|Compound:3',6'-dihydroxyflavone|FD-Score:5.56|P-value:4.16E-9 ID:SGTC_2066|Compound:5222451|FD-Score:4.77|P-value:3.81E-7 ID:SGTC_2080|Compound:5212524|FD-Score:-4.22|P-value:5.97E-6 ID:SGTC_2081|Compound:5214970|FD-Score:-3.92|P-value:2.35E-5 ID:SGTC_2308|Compound:7584454|FD-Score:-3.39|P-value:2.15E-4 ID:SGTC_2416|Compound:tofa|FD-Score:3.82|P-value:3.67E-5 ID:SGTC_2421|Compound:5690543|FD-Score:3.33|P-value:2.74E-4 ID:SGTC_2570|Compound:licareol|FD-Score:4.49|P-value:1.58E-6 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:3.22|P-value:4.25E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:3.16|P-value:5.23E-4 ID:SGTC_2600|Compound:tangeritin|FD-Score:4.64|P-value:7.52E-7 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:6.26|P-value:4.13E-11 ID:SGTC_2618|Compound:avocadyne|FD-Score:7.80|P-value:2.93E-16 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:11.80|P-value:9.28E-35 ID:SGTC_2803|Compound:7707263|FD-Score:3.62|P-value:8.56E-5 ID:SGTC_2844|Compound:9010103|FD-Score:3.15|P-value:5.39E-4 ID:SGTC_2890|Compound:9046729|FD-Score:5.31|P-value:1.83E-8 ID:SGTC_2924|Compound:9010168|FD-Score:3.94|P-value:2.18E-5 ID:SGTC_2975|Compound:9084847|FD-Score:3.43|P-value:1.91E-4 ID:SGTC_3046|Compound:9092212|FD-Score:3.17|P-value:5.01E-4 ID:SGTC_3151|Compound:9099821|FD-Score:3.33|P-value:2.76E-4 ID:SGTC_3157|Compound:9099274|FD-Score:3.01|P-value:8.89E-4 ID:SGTC_3221|Compound:9128209|FD-Score:3.88|P-value:2.88E-5 ID:SGTC_3224|Compound:9130643|FD-Score:-3.44|P-value:1.80E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.29|P-value:3.19E-4 ID:SGTC_112|Compound:phenothiazine|FD-Score:-3.03|P-value:8.29E-4 ID:SGTC_113|Compound:0099-0221|FD-Score:-3.44|P-value:1.75E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:10.80|P-value:1.70E-29 ID:SGTC_424|Compound:splitomicin|FD-Score:4.15|P-value:8.30E-6 ID:SGTC_513|Compound:4245-1575|FD-Score:-3.50|P-value:1.39E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:4.05|P-value:1.31E-5 ID:SGTC_541|Compound:4513-1321|FD-Score:3.08|P-value:7.07E-4 ID:SGTC_722|Compound:3534-0461|FD-Score:3.31|P-value:2.98E-4 ID:SGTC_752|Compound:1896-0090|FD-Score:4.02|P-value:1.52E-5 ID:SGTC_821|Compound:1114-0266|FD-Score:3.24|P-value:3.95E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:4.12|P-value:9.79E-6 ID:SGTC_914|Compound:3820-6779|FD-Score:4.41|P-value:2.45E-6 ID:SGTC_1103|Compound:duloxetine|FD-Score:4.11|P-value:1.02E-5 ID:SGTC_1105|Compound:epirubicin|FD-Score:3.32|P-value:2.92E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.55|P-value:1.16E-4 ID:SGTC_1234|Compound:0326-0494|FD-Score:3.84|P-value:3.36E-5 ID:SGTC_1311|Compound:1190-0148|FD-Score:3.22|P-value:4.21E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:3.07|P-value:7.35E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:-2.98|P-value:9.90E-4 ID:SGTC_1570|Compound:verbenone|FD-Score:3.77|P-value:4.56E-5 ID:SGTC_1759|Compound:st043059|FD-Score:4.19|P-value:6.98E-6 ID:SGTC_1849|Compound:st056239|FD-Score:-3.22|P-value:4.15E-4 ID:SGTC_1894|Compound:3',6'-dihydroxyflavone|FD-Score:5.56|P-value:4.16E-9 ID:SGTC_2066|Compound:5222451|FD-Score:4.77|P-value:3.81E-7 ID:SGTC_2080|Compound:5212524|FD-Score:-4.22|P-value:5.97E-6 ID:SGTC_2081|Compound:5214970|FD-Score:-3.92|P-value:2.35E-5 ID:SGTC_2308|Compound:7584454|FD-Score:-3.39|P-value:2.15E-4 ID:SGTC_2416|Compound:tofa|FD-Score:3.82|P-value:3.67E-5 ID:SGTC_2421|Compound:5690543|FD-Score:3.33|P-value:2.74E-4 ID:SGTC_2570|Compound:licareol|FD-Score:4.49|P-value:1.58E-6 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:3.22|P-value:4.25E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:3.16|P-value:5.23E-4 ID:SGTC_2600|Compound:tangeritin|FD-Score:4.64|P-value:7.52E-7 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:6.26|P-value:4.13E-11 ID:SGTC_2618|Compound:avocadyne|FD-Score:7.80|P-value:2.93E-16 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:11.80|P-value:9.28E-35 ID:SGTC_2803|Compound:7707263|FD-Score:3.62|P-value:8.56E-5 ID:SGTC_2844|Compound:9010103|FD-Score:3.15|P-value:5.39E-4 ID:SGTC_2890|Compound:9046729|FD-Score:5.31|P-value:1.83E-8 ID:SGTC_2924|Compound:9010168|FD-Score:3.94|P-value:2.18E-5 ID:SGTC_2975|Compound:9084847|FD-Score:3.43|P-value:1.91E-4 ID:SGTC_3046|Compound:9092212|FD-Score:3.17|P-value:5.01E-4 ID:SGTC_3151|Compound:9099821|FD-Score:3.33|P-value:2.76E-4 ID:SGTC_3157|Compound:9099274|FD-Score:3.01|P-value:8.89E-4 ID:SGTC_3221|Compound:9128209|FD-Score:3.88|P-value:2.88E-5 ID:SGTC_3224|Compound:9130643|FD-Score:-3.44|P-value:1.80E-4

Top fitness defect scores for YBL032W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2626 ethyl orsellinate 11.80 100.0 µM 9.28E-35 1 1.87E-32
2 SGTC_115 0527-0192 10.80 112.0 µM 1.70E-29 4 1.47E-27
3 SGTC_2618 avocadyne 7.80 43.4 µM 2.93E-16 14 3.02E-15
4 SGTC_2617 dihydrotanshinone i 6.26 20.8 µM 4.13E-11 copper-dependent oxidative stress 12 1.87E-10
5 SGTC_1894 3',6'-dihydroxyflavone 5.56 78.7 µM 4.16E-9 3 1.38E-8
6 SGTC_2890 9046729 5.31 58.4 µM 1.83E-8 Golgi 11 5.48E-8
7 SGTC_2066 5222451 4.77 176.0 µM 3.81E-7 heme biosynthesis & mitochondrial translocase 15 9.25E-7
8 SGTC_2600 tangeritin 4.64 88.0 µM 7.52E-7 17 1.74E-6
9 SGTC_2570 licareol 4.49 100.0 µM 1.58E-6 17 3.49E-6
10 SGTC_914 3820-6779 4.41 408.0 µM 2.45E-6 14 5.25E-6
11 SGTC_1759 st043059 4.19 70.0 µM 6.98E-6 amide catabolism 19 1.39E-5
12 SGTC_424 splitomicin 4.15 100.0 mM 8.30E-6 18 1.64E-5
13 SGTC_832 4083-0019 4.12 164.0 µM 9.79E-6 translation 86 1.91E-5
14 SGTC_1103 duloxetine 4.11 36.1 µM 1.02E-5 25 1.99E-5
15 SGTC_528 3492-0019 4.05 172.0 µM 1.31E-5 calcium & mitochondrial duress 211 2.51E-5
16 SGTC_752 1896-0090 4.02 130.0 µM 1.52E-5 TSC3-RPN4 16 2.88E-5
17 SGTC_2924 9010168 3.94 62.8 µM 2.18E-5 cell wall signaling 39 4.03E-5
18 SGTC_3221 9128209 3.88 49.5 µM 2.88E-5 14 5.23E-5
19 SGTC_1234 0326-0494 3.84 15.5 µM 3.36E-5 16 6.04E-5
20 SGTC_2416 tofa 3.82 880.0 nM 3.67E-5 sphingolipid biosynthesis & PDR1 55 6.56E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.150 1.88E-18 YLL001W DNM1 hom Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance
0.139 6.82E-16 YDL072C YET3 hom Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress
0.139 7.07E-16 YHR205W SCH9 hom AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan
0.132 1.96E-14 YDR297W SUR2 hom Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis
0.109 2.77E-10 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.109 2.79E-10 YEL029C BUD16 hom Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK)
0.109 2.80E-10 YGL049C TIF4632 hom Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication
0.101 4.93E-9 YKR016W FCJ1 hom Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane
0.095 3.16E-8 YBR238C YBR238C hom Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span
0.091 1.12E-7 YOR339C UBC11 hom Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2
0.090 1.52E-7 YJL079C PRY1 hom Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication
0.089 2.10E-7 YNL250W RAD50 hom Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress
0.089 2.13E-7 YKR028W SAP190 hom Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication
0.088 3.01E-7 YOL163W_p YOL163W_p hom Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family
0.088 3.31E-7 YJL164C TPK1 hom cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication