YBL036C

Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS

Zygosity: Homozygous strain
fixedexpanded
Profile for YBL036C / YBL036C

Click on Significant Values for Screen Details ID:SGTC_189|Compound:3970-0742|FD-Score:2.77|P-value:9.12E-4 ID:SGTC_560|Compound:2332-1943|FD-Score:-3.16|P-value:2.92E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-3.53|P-value:6.01E-5 ID:SGTC_614|Compound:0462-0418|FD-Score:-3.73|P-value:2.33E-5 ID:SGTC_630|Compound:4356-0227|FD-Score:-3.47|P-value:7.67E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.40|P-value:1.04E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:-2.97|P-value:6.08E-4 ID:SGTC_982|Compound:1491-0518|FD-Score:-2.95|P-value:6.69E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-5.93|P-value:3.97E-11 ID:SGTC_1251|Compound:0490-5699|FD-Score:3.06|P-value:2.88E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:-3.75|P-value:2.16E-5 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:2.95|P-value:4.55E-4 ID:SGTC_1630|Compound:st005209|FD-Score:3.46|P-value:5.26E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-4.42|P-value:6.50E-7 ID:SGTC_1986|Compound:st071812|FD-Score:-3.32|P-value:1.46E-4 ID:SGTC_2103|Compound:5378138|FD-Score:2.84|P-value:6.86E-4 ID:SGTC_2146|Compound:5545642|FD-Score:-3.24|P-value:2.04E-4 ID:SGTC_2160|Compound:5660466|FD-Score:2.92|P-value:5.02E-4 ID:SGTC_2332|Compound:7982454|FD-Score:-3.00|P-value:5.42E-4 ID:SGTC_2602|Compound:kawain|FD-Score:3.29|P-value:1.08E-4 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:-3.22|P-value:2.28E-4 ID:SGTC_2692|Compound:st077426|FD-Score:3.00|P-value:3.76E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.07|P-value:2.83E-4 ID:SGTC_3206|Compound:9114350|FD-Score:-3.12|P-value:3.37E-4 ID:SGTC_3297|Compound:9117502|FD-Score:2.98|P-value:4.09E-4 ID:SGTC_189|Compound:3970-0742|FD-Score:2.77|P-value:9.12E-4 ID:SGTC_560|Compound:2332-1943|FD-Score:-3.16|P-value:2.92E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-3.53|P-value:6.01E-5 ID:SGTC_614|Compound:0462-0418|FD-Score:-3.73|P-value:2.33E-5 ID:SGTC_630|Compound:4356-0227|FD-Score:-3.47|P-value:7.67E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.40|P-value:1.04E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:-2.97|P-value:6.08E-4 ID:SGTC_982|Compound:1491-0518|FD-Score:-2.95|P-value:6.69E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-5.93|P-value:3.97E-11 ID:SGTC_1251|Compound:0490-5699|FD-Score:3.06|P-value:2.88E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:-3.75|P-value:2.16E-5 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:2.95|P-value:4.55E-4 ID:SGTC_1630|Compound:st005209|FD-Score:3.46|P-value:5.26E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-4.42|P-value:6.50E-7 ID:SGTC_1986|Compound:st071812|FD-Score:-3.32|P-value:1.46E-4 ID:SGTC_2103|Compound:5378138|FD-Score:2.84|P-value:6.86E-4 ID:SGTC_2146|Compound:5545642|FD-Score:-3.24|P-value:2.04E-4 ID:SGTC_2160|Compound:5660466|FD-Score:2.92|P-value:5.02E-4 ID:SGTC_2332|Compound:7982454|FD-Score:-3.00|P-value:5.42E-4 ID:SGTC_2602|Compound:kawain|FD-Score:3.29|P-value:1.08E-4 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:-3.22|P-value:2.28E-4 ID:SGTC_2692|Compound:st077426|FD-Score:3.00|P-value:3.76E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.07|P-value:2.83E-4 ID:SGTC_3206|Compound:9114350|FD-Score:-3.12|P-value:3.37E-4 ID:SGTC_3297|Compound:9117502|FD-Score:2.98|P-value:4.09E-4

Top fitness defect scores for YBL036C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1630 st005209 3.46 31.3 µM 5.26E-5 41 2.74E-4
2 SGTC_2602 kawain 3.29 100.0 µM 1.08E-4 53 4.93E-4
3 SGTC_2794 7785382 3.07 27.3 µM 2.83E-4 87 0.00108
4 SGTC_1251 0490-5699 3.06 265.0 µM 2.88E-4 80 0.00110
5 SGTC_2692 st077426 3.00 47.7 µM 3.76E-4 68 0.00137
6 SGTC_3297 9117502 2.98 71.4 µM 4.09E-4 36 0.00146
7 SGTC_1587 estradiol valerate 2.95 56.1 µM 4.55E-4 44 0.00160
8 SGTC_2160 5660466 2.92 132.9 µM 5.02E-4 60S ribosome export 53 0.00173
9 SGTC_2103 5378138 2.84 10.0 µM 6.86E-4 71 0.00223
10 SGTC_189 3970-0742 2.77 2.1 µM 9.12E-4 40 0.00282
11 SGTC_366 0181-0206 2.70 4.3 µM 0.00118 Golgi 126 0.00348
12 SGTC_606 k015-0008 2.67 38.6 µM 0.00131 90 0.00378
13 SGTC_2791 5663188 2.64 71.4 µM 0.00148 RPP1 & pyrimidine depletion 97 0.00420
14 SGTC_73 0327-0325 2.61 16.2 µM 0.00161 DNA damage response 169 0.00450
15 SGTC_2493 resveratrol 2.59 26.1 µM 0.00175 65 0.00480
16 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 2.56 3.7 µM 0.00195 296 0.00525
17 SGTC_381 3461-2219 2.51 42.7 µM 0.00228 copper-dependent oxidative stress 102 0.00597
18 SGTC_3018 9082602 2.50 49.5 µM 0.00240 124 0.00623
19 SGTC_186 3455-3465 2.49 67.3 µM 0.00247 36 0.00638
20 SGTC_1988 st071872 2.49 53.4 µM 0.00249 94 0.00641

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.423 1.66E-145 YOL141W PPM2 hom AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p
0.371 5.42E-110 YLR205C HMX1 hom ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
0.354 1.76E-99 YLL012W YEH1 hom Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
0.344 8.89E-94 YBL015W ACH1 hom Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth
0.342 1.60E-92 YKL220C FRE2 hom Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels
0.338 2.23E-90 YKL179C COY1 hom Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.328 4.84E-85 YBR262C AIM5 hom Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
0.323 1.69E-82 YER064C VHR2 hom Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.318 1.01E-79 YAL045C_d YAL045C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A
0.317 2.07E-79 YML083C YML083C hom Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions
0.313 3.74E-77 YGL081W_p YGL081W_p hom Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis
0.312 6.66E-77 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.312 1.50E-76 YDL110C TMA17 hom Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion
0.284 3.75E-63 YER060W-A FCY22 hom Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function
0.282 1.86E-62 YMR095C SNO1 hom Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase