YBR025C / OLA1

P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR025C / OLA1

Click on Significant Values for Screen Details ID:SGTC_47|Compound:0149-0135|FD-Score:3.56|P-value:2.00E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.87|P-value:5.72E-5 ID:SGTC_77|Compound:0828-0286|FD-Score:4.04|P-value:2.97E-5 ID:SGTC_78|Compound:0336-0123|FD-Score:9.73|P-value:1.81E-22 ID:SGTC_103|Compound:0844-0104|FD-Score:3.56|P-value:2.03E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:3.12|P-value:9.66E-4 ID:SGTC_544|Compound:k015-0020|FD-Score:4.89|P-value:5.94E-7 ID:SGTC_547|Compound:1120-0019|FD-Score:-3.15|P-value:8.46E-4 ID:SGTC_553|Compound:k007-0256|FD-Score:5.74|P-value:5.65E-9 ID:SGTC_554|Compound:4483-2211|FD-Score:5.90|P-value:2.18E-9 ID:SGTC_560|Compound:2332-1943|FD-Score:4.14|P-value:1.98E-5 ID:SGTC_814|Compound:3342-0569|FD-Score:-3.38|P-value:3.73E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:5.64|P-value:1.00E-8 ID:SGTC_939|Compound:1165-0479|FD-Score:-4.21|P-value:1.34E-5 ID:SGTC_941|Compound:3237-0776|FD-Score:3.84|P-value:6.87E-5 ID:SGTC_942|Compound:3253-1115|FD-Score:4.77|P-value:1.08E-6 ID:SGTC_1052|Compound:3253-3998|FD-Score:3.15|P-value:8.65E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:5.10|P-value:2.01E-7 ID:SGTC_1068|Compound:loratadine|FD-Score:3.36|P-value:4.24E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:4.11|P-value:2.20E-5 ID:SGTC_1449|Compound:4112-4002|FD-Score:3.53|P-value:2.25E-4 ID:SGTC_1470|Compound:k295-0292|FD-Score:3.80|P-value:8.09E-5 ID:SGTC_1489|Compound:4065-0444|FD-Score:3.17|P-value:8.20E-4 ID:SGTC_1600|Compound:coumarin 106|FD-Score:3.51|P-value:2.43E-4 ID:SGTC_1605|Compound:st000082|FD-Score:3.79|P-value:8.15E-5 ID:SGTC_1617|Compound:st002906|FD-Score:3.62|P-value:1.58E-4 ID:SGTC_1784|Compound:st039334|FD-Score:3.75|P-value:9.83E-5 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.96|P-value:4.22E-5 ID:SGTC_1786|Compound:berberine|FD-Score:3.54|P-value:2.20E-4 ID:SGTC_1912|Compound:st060209|FD-Score:4.52|P-value:3.54E-6 ID:SGTC_2020|Compound:4017018|FD-Score:3.60|P-value:1.72E-4 ID:SGTC_2021|Compound:4019055|FD-Score:-4.06|P-value:2.60E-5 ID:SGTC_2022|Compound:5136654|FD-Score:-3.94|P-value:4.27E-5 ID:SGTC_2077|Compound:5212015|FD-Score:-3.12|P-value:9.20E-4 ID:SGTC_2332|Compound:7982454|FD-Score:3.48|P-value:2.70E-4 ID:SGTC_2363|Compound:9053361|FD-Score:-4.77|P-value:9.65E-7 ID:SGTC_2367|Compound:9070478|FD-Score:3.78|P-value:8.59E-5 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:3.70|P-value:1.19E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:3.17|P-value:8.24E-4 ID:SGTC_2964|Compound:9083345|FD-Score:5.25|P-value:9.12E-8 ID:SGTC_3057|Compound:9095946|FD-Score:3.40|P-value:3.64E-4 ID:SGTC_3140|Compound:9094243|FD-Score:5.10|P-value:1.95E-7 ID:SGTC_3238|Compound:9133470|FD-Score:-3.60|P-value:1.64E-4 ID:SGTC_47|Compound:0149-0135|FD-Score:3.56|P-value:2.00E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.87|P-value:5.72E-5 ID:SGTC_77|Compound:0828-0286|FD-Score:4.04|P-value:2.97E-5 ID:SGTC_78|Compound:0336-0123|FD-Score:9.73|P-value:1.81E-22 ID:SGTC_103|Compound:0844-0104|FD-Score:3.56|P-value:2.03E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:3.12|P-value:9.66E-4 ID:SGTC_544|Compound:k015-0020|FD-Score:4.89|P-value:5.94E-7 ID:SGTC_547|Compound:1120-0019|FD-Score:-3.15|P-value:8.46E-4 ID:SGTC_553|Compound:k007-0256|FD-Score:5.74|P-value:5.65E-9 ID:SGTC_554|Compound:4483-2211|FD-Score:5.90|P-value:2.18E-9 ID:SGTC_560|Compound:2332-1943|FD-Score:4.14|P-value:1.98E-5 ID:SGTC_814|Compound:3342-0569|FD-Score:-3.38|P-value:3.73E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:5.64|P-value:1.00E-8 ID:SGTC_939|Compound:1165-0479|FD-Score:-4.21|P-value:1.34E-5 ID:SGTC_941|Compound:3237-0776|FD-Score:3.84|P-value:6.87E-5 ID:SGTC_942|Compound:3253-1115|FD-Score:4.77|P-value:1.08E-6 ID:SGTC_1052|Compound:3253-3998|FD-Score:3.15|P-value:8.65E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:5.10|P-value:2.01E-7 ID:SGTC_1068|Compound:loratadine|FD-Score:3.36|P-value:4.24E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:4.11|P-value:2.20E-5 ID:SGTC_1449|Compound:4112-4002|FD-Score:3.53|P-value:2.25E-4 ID:SGTC_1470|Compound:k295-0292|FD-Score:3.80|P-value:8.09E-5 ID:SGTC_1489|Compound:4065-0444|FD-Score:3.17|P-value:8.20E-4 ID:SGTC_1600|Compound:coumarin 106|FD-Score:3.51|P-value:2.43E-4 ID:SGTC_1605|Compound:st000082|FD-Score:3.79|P-value:8.15E-5 ID:SGTC_1617|Compound:st002906|FD-Score:3.62|P-value:1.58E-4 ID:SGTC_1784|Compound:st039334|FD-Score:3.75|P-value:9.83E-5 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.96|P-value:4.22E-5 ID:SGTC_1786|Compound:berberine|FD-Score:3.54|P-value:2.20E-4 ID:SGTC_1912|Compound:st060209|FD-Score:4.52|P-value:3.54E-6 ID:SGTC_2020|Compound:4017018|FD-Score:3.60|P-value:1.72E-4 ID:SGTC_2021|Compound:4019055|FD-Score:-4.06|P-value:2.60E-5 ID:SGTC_2022|Compound:5136654|FD-Score:-3.94|P-value:4.27E-5 ID:SGTC_2077|Compound:5212015|FD-Score:-3.12|P-value:9.20E-4 ID:SGTC_2332|Compound:7982454|FD-Score:3.48|P-value:2.70E-4 ID:SGTC_2363|Compound:9053361|FD-Score:-4.77|P-value:9.65E-7 ID:SGTC_2367|Compound:9070478|FD-Score:3.78|P-value:8.59E-5 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:3.70|P-value:1.19E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:3.17|P-value:8.24E-4 ID:SGTC_2964|Compound:9083345|FD-Score:5.25|P-value:9.12E-8 ID:SGTC_3057|Compound:9095946|FD-Score:3.40|P-value:3.64E-4 ID:SGTC_3140|Compound:9094243|FD-Score:5.10|P-value:1.95E-7 ID:SGTC_3238|Compound:9133470|FD-Score:-3.60|P-value:1.64E-4

Top fitness defect scores for YBR025C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_78 0336-0123 9.73 156.8 µM 1.81E-22 1 1.10E-22
2 SGTC_554 4483-2211 5.90 187.0 µM 2.18E-9 3 1.78E-9
3 SGTC_553 k007-0256 5.74 45.7 µM 5.65E-9 heme biosynthesis & mitochondrial translocase 7 4.66E-9
4 SGTC_922 1866-0035 5.64 480.0 µM 1.00E-8 excess fatty acid 23 8.32E-9
5 SGTC_2964 9083345 5.25 53.4 µM 9.12E-8 7 7.73E-8
6 SGTC_3140 9094243 5.10 49.5 µM 1.95E-7 12 1.66E-7
7 SGTC_1053 3366-3204 5.10 134.0 µM 2.01E-7 cell wall 14 1.72E-7
8 SGTC_544 k015-0020 4.89 52.4 µM 5.94E-7 6 5.13E-7
9 SGTC_942 3253-1115 4.77 227.0 µM 1.08E-6 9 9.37E-7
10 SGTC_1912 st060209 4.52 90.8 µM 3.54E-6 25 3.11E-6
11 SGTC_560 2332-1943 4.14 155.0 µM 1.98E-5 7 1.77E-5
12 SGTC_1385 3091-4834 4.11 14.1 µM 2.20E-5 RPP1 & pyrimidine depletion 17 1.97E-5
13 SGTC_77 0828-0286 4.04 12.5 µM 2.97E-5 TSC3-RPN4 33 2.67E-5
14 SGTC_1785 bathophenanthroline 3.96 24.0 µM 4.22E-5 heme requiring 47 3.81E-5
15 SGTC_941 3237-0776 3.84 460.0 µM 6.87E-5 17 6.22E-5
16 SGTC_1470 k295-0292 3.80 231.0 µM 8.09E-5 15 7.34E-5
17 SGTC_1605 st000082 3.79 49.1 µM 8.15E-5 60S ribosome export 18 7.39E-5
18 SGTC_2367 9070478 3.78 135.4 µM 8.59E-5 cell wall 38 7.80E-5
19 SGTC_1784 st039334 3.75 36.3 µM 9.83E-5 23 8.94E-5
20 SGTC_2577 2',4'-dihydroxy-4-methoxychalcone 3.70 15.3 µM 1.19E-4 42 1.08E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.111 1.29E-10 YKR064W OAF3 hom Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress
0.110 2.02E-10 YDL097C RPN6 het Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
0.102 2.77E-9 YDR111C_p ALT2_p hom Catalytically inactive paralog of ALT1, an alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p
0.094 4.56E-8 YKR037C SPC34 het Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
0.093 7.12E-8 YKR043C SHB17 hom Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus
0.092 7.99E-8 YMR306C-A_d YMR306C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.090 1.75E-7 YDR363W ESC2 hom Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member
0.089 2.29E-7 YMR030W RSF1 hom Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes
0.089 2.66E-7 YBL008W-A_p YBL008W-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.089 2.61E-7 YPL117C IDI1 het Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability
0.085 9.40E-7 YOR227W HER1 hom Protein of unknown function required for proliferation or remodeling of the ER that is caused by overexpression of Hmg2p; may interact with ribosomes, based on co-purification experiments; HER1 has a paralog, GIP3, that arose from the whole genome duplication
0.084 1.18E-6 YPR059C_d YPR059C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W
0.082 2.27E-6 YER073W ALD5 hom Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed
0.080 3.32E-6 YBR247C ENP1 het Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus
0.080 3.72E-6 YBR086C IST2 hom Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process