HIPHOP chemogenomics database

YBR030W / RKM3

Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein

Zygosity: Homozygous strain

fixedexpanded
Profile for YBR030W / RKM3

Click on Significant Values for Screen Details

Top fitness defect scores for YBR030W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_937	3122-0005	6.19	46.7 µM	1.50E-11		6	3.10E-10
2	SGTC_731	3966-0296	5.93	387.0 µM	9.45E-11		2	1.55E-9
3	SGTC_1885	st057546	4.36	99.9 µM	1.29E-6		13	6.51E-6
4	SGTC_141	0443-0274	4.19	32.3 µM	3.06E-6	mitochondrial response to ROS	35	1.38E-5
5	SGTC_1724	st037281	4.18	8.3 µM	3.22E-6		37	1.45E-5
6	SGTC_668	1682-0499	4.03	62.6 µM	6.66E-6		20	2.74E-5
7	SGTC_136	0097-0015	4.03	404.5 µM	6.91E-6	60S ribosome export	15	2.83E-5
8	SGTC_2646	retinol	3.81	96.8 µM	1.95E-5		14	7.01E-5
9	SGTC_691	1548-0686	3.61	185.0 µM	4.84E-5		52	1.55E-4
10	SGTC_924	2046-0044	3.45	21.3 µM	9.46E-5		23	2.79E-4
11	SGTC_686	1319-0111	3.41	510.0 µM	1.12E-4	60S ribosome export	46	3.25E-4
12	SGTC_611	3453-1652	3.40	23.7 µM	1.19E-4		37	3.41E-4
13	SGTC_1573	oleylamine	3.39	1.1 µM	1.24E-4		51	3.54E-4
14	SGTC_2377	9073351	3.32	62.6 µM	1.61E-4	ergosterol biosynthesis	44	4.45E-4
15	SGTC_713	1488-1073	3.31	324.0 µM	1.73E-4	ERG2	27	4.74E-4
16	SGTC_151	3013-0144	3.29	2.3 µM	1.88E-4	iron homeostasis	54	5.09E-4
17	SGTC_1609	st001574	3.28	46.4 µM	1.91E-4		32	5.18E-4
18	SGTC_2547	epiafzelechin trimethyl ether	3.27	100.0 µM	2.00E-4		6	5.39E-4
19	SGTC_2209	5-(5-methyl-2-thenylidene)-Barbituric acid	3.16	122.8 µM	3.10E-4		26	7.89E-4
20	SGTC_1606	st000094	3.13	49.2 µM	3.44E-4	60S ribosome export	46	8.65E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.107	5.38E-10	YHR075C	PPE1	hom	Protein with carboxyl methyl esterase activity that may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein
0.097	1.80E-8	YDR134C	YDR134C	hom	Hypothetical protein; YDR134C has a paralog, CCW12, that arose from the whole genome duplication
0.091	1.38E-7	YDR510W	SMT3	het	Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2
0.089	2.37E-7	YDR308C	SRB7	het	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p
0.087	4.84E-7	YNL191W	DUG3	hom	Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p)
0.082	2.10E-6	YGR125W_p	YGR125W_p	hom	Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
0.078	6.15E-6	YKR024C	DBP7	hom	Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions
0.073	2.37E-5	YPL090C	RPS6A	hom	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication
0.071	3.67E-5	YKL071W_p	YKL071W_p	hom	Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.070	4.91E-5	YLR061W	RPL22A	hom	Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication
0.066	1.22E-4	YAL017W	PSK1	hom	PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication
0.064	1.92E-4	YNL329C	PEX6	hom	AAA-peroxin that heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol
0.064	2.18E-4	YNL303W_d	YNL303W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.063	2.80E-4	YNL209W	SSB2	hom	Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; SSB2 has a paralog, SSB1, that arose from the whole genome duplication
0.062	3.35E-4	YER091C-A_d	YER091C-A_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data