YBR030W / RKM3

Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR030W / RKM3

Click on Significant Values for Screen Details ID:SGTC_132|Compound:0417-1715|FD-Score:-3.16|P-value:4.58E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:4.03|P-value:6.91E-6 ID:SGTC_141|Compound:0443-0274|FD-Score:4.19|P-value:3.06E-6 ID:SGTC_151|Compound:3013-0144|FD-Score:3.29|P-value:1.88E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:-3.56|P-value:9.36E-5 ID:SGTC_419|Compound:nsc-638432|FD-Score:2.95|P-value:7.02E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:-3.91|P-value:2.02E-5 ID:SGTC_611|Compound:3453-1652|FD-Score:3.40|P-value:1.19E-4 ID:SGTC_668|Compound:1682-0499|FD-Score:4.03|P-value:6.66E-6 ID:SGTC_686|Compound:1319-0111|FD-Score:3.41|P-value:1.12E-4 ID:SGTC_691|Compound:1548-0686|FD-Score:3.61|P-value:4.84E-5 ID:SGTC_713|Compound:1488-1073|FD-Score:3.31|P-value:1.73E-4 ID:SGTC_731|Compound:3966-0296|FD-Score:5.93|P-value:9.45E-11 ID:SGTC_826|Compound:1683-5028|FD-Score:3.04|P-value:4.90E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:-3.22|P-value:3.68E-4 ID:SGTC_924|Compound:2046-0044|FD-Score:3.45|P-value:9.46E-5 ID:SGTC_937|Compound:3122-0005|FD-Score:6.19|P-value:1.50E-11 ID:SGTC_1156|Compound:4092-0367|FD-Score:-3.39|P-value:1.89E-4 ID:SGTC_1418|Compound:3966-3099|FD-Score:-3.34|P-value:2.33E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:3.39|P-value:1.24E-4 ID:SGTC_1606|Compound:st000094|FD-Score:3.13|P-value:3.44E-4 ID:SGTC_1609|Compound:st001574|FD-Score:3.28|P-value:1.91E-4 ID:SGTC_1623|Compound:st003709|FD-Score:-3.89|P-value:2.21E-5 ID:SGTC_1724|Compound:st037281|FD-Score:4.18|P-value:3.22E-6 ID:SGTC_1885|Compound:st057546|FD-Score:4.36|P-value:1.29E-6 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:3.16|P-value:3.10E-4 ID:SGTC_2259|Compound:7964009|FD-Score:2.98|P-value:6.27E-4 ID:SGTC_2263|Compound:7948595|FD-Score:2.95|P-value:6.91E-4 ID:SGTC_2349|Compound:9023730|FD-Score:-3.19|P-value:4.20E-4 ID:SGTC_2363|Compound:9053361|FD-Score:2.86|P-value:9.45E-4 ID:SGTC_2377|Compound:9073351|FD-Score:3.32|P-value:1.61E-4 ID:SGTC_2477|Compound:5967686|FD-Score:2.99|P-value:5.96E-4 ID:SGTC_2547|Compound:epiafzelechin trimethyl ether|FD-Score:3.27|P-value:2.00E-4 ID:SGTC_2646|Compound:retinol|FD-Score:3.81|P-value:1.95E-5 ID:SGTC_2805|Compound:7950783|FD-Score:-3.16|P-value:4.57E-4 ID:SGTC_2810|Compound:7959871|FD-Score:-2.96|P-value:9.87E-4 ID:SGTC_2854|Compound:9024559|FD-Score:-4.12|P-value:7.40E-6 ID:SGTC_2884|Compound:9058502|FD-Score:2.86|P-value:9.40E-4 ID:SGTC_2914|Compound:7968218|FD-Score:3.12|P-value:3.62E-4 ID:SGTC_2950|Compound:9072895|FD-Score:-3.00|P-value:8.55E-4 ID:SGTC_2958|Compound:9077254|FD-Score:2.90|P-value:8.15E-4 ID:SGTC_3007|Compound:9078383|FD-Score:-4.30|P-value:3.05E-6 ID:SGTC_3159|Compound:9100096|FD-Score:3.02|P-value:5.37E-4 ID:SGTC_3352|Compound:9151559|FD-Score:-2.99|P-value:8.72E-4 ID:SGTC_132|Compound:0417-1715|FD-Score:-3.16|P-value:4.58E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:4.03|P-value:6.91E-6 ID:SGTC_141|Compound:0443-0274|FD-Score:4.19|P-value:3.06E-6 ID:SGTC_151|Compound:3013-0144|FD-Score:3.29|P-value:1.88E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:-3.56|P-value:9.36E-5 ID:SGTC_419|Compound:nsc-638432|FD-Score:2.95|P-value:7.02E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:-3.91|P-value:2.02E-5 ID:SGTC_611|Compound:3453-1652|FD-Score:3.40|P-value:1.19E-4 ID:SGTC_668|Compound:1682-0499|FD-Score:4.03|P-value:6.66E-6 ID:SGTC_686|Compound:1319-0111|FD-Score:3.41|P-value:1.12E-4 ID:SGTC_691|Compound:1548-0686|FD-Score:3.61|P-value:4.84E-5 ID:SGTC_713|Compound:1488-1073|FD-Score:3.31|P-value:1.73E-4 ID:SGTC_731|Compound:3966-0296|FD-Score:5.93|P-value:9.45E-11 ID:SGTC_826|Compound:1683-5028|FD-Score:3.04|P-value:4.90E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:-3.22|P-value:3.68E-4 ID:SGTC_924|Compound:2046-0044|FD-Score:3.45|P-value:9.46E-5 ID:SGTC_937|Compound:3122-0005|FD-Score:6.19|P-value:1.50E-11 ID:SGTC_1156|Compound:4092-0367|FD-Score:-3.39|P-value:1.89E-4 ID:SGTC_1418|Compound:3966-3099|FD-Score:-3.34|P-value:2.33E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:3.39|P-value:1.24E-4 ID:SGTC_1606|Compound:st000094|FD-Score:3.13|P-value:3.44E-4 ID:SGTC_1609|Compound:st001574|FD-Score:3.28|P-value:1.91E-4 ID:SGTC_1623|Compound:st003709|FD-Score:-3.89|P-value:2.21E-5 ID:SGTC_1724|Compound:st037281|FD-Score:4.18|P-value:3.22E-6 ID:SGTC_1885|Compound:st057546|FD-Score:4.36|P-value:1.29E-6 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:3.16|P-value:3.10E-4 ID:SGTC_2259|Compound:7964009|FD-Score:2.98|P-value:6.27E-4 ID:SGTC_2263|Compound:7948595|FD-Score:2.95|P-value:6.91E-4 ID:SGTC_2349|Compound:9023730|FD-Score:-3.19|P-value:4.20E-4 ID:SGTC_2363|Compound:9053361|FD-Score:2.86|P-value:9.45E-4 ID:SGTC_2377|Compound:9073351|FD-Score:3.32|P-value:1.61E-4 ID:SGTC_2477|Compound:5967686|FD-Score:2.99|P-value:5.96E-4 ID:SGTC_2547|Compound:epiafzelechin trimethyl ether|FD-Score:3.27|P-value:2.00E-4 ID:SGTC_2646|Compound:retinol|FD-Score:3.81|P-value:1.95E-5 ID:SGTC_2805|Compound:7950783|FD-Score:-3.16|P-value:4.57E-4 ID:SGTC_2810|Compound:7959871|FD-Score:-2.96|P-value:9.87E-4 ID:SGTC_2854|Compound:9024559|FD-Score:-4.12|P-value:7.40E-6 ID:SGTC_2884|Compound:9058502|FD-Score:2.86|P-value:9.40E-4 ID:SGTC_2914|Compound:7968218|FD-Score:3.12|P-value:3.62E-4 ID:SGTC_2950|Compound:9072895|FD-Score:-3.00|P-value:8.55E-4 ID:SGTC_2958|Compound:9077254|FD-Score:2.90|P-value:8.15E-4 ID:SGTC_3007|Compound:9078383|FD-Score:-4.30|P-value:3.05E-6 ID:SGTC_3159|Compound:9100096|FD-Score:3.02|P-value:5.37E-4 ID:SGTC_3352|Compound:9151559|FD-Score:-2.99|P-value:8.72E-4

Top fitness defect scores for YBR030W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_937 3122-0005 6.19 46.7 µM 1.50E-11 6 3.10E-10
2 SGTC_731 3966-0296 5.93 387.0 µM 9.45E-11 2 1.55E-9
3 SGTC_1885 st057546 4.36 99.9 µM 1.29E-6 13 6.51E-6
4 SGTC_141 0443-0274 4.19 32.3 µM 3.06E-6 mitochondrial response to ROS 35 1.38E-5
5 SGTC_1724 st037281 4.18 8.3 µM 3.22E-6 37 1.45E-5
6 SGTC_668 1682-0499 4.03 62.6 µM 6.66E-6 20 2.74E-5
7 SGTC_136 0097-0015 4.03 404.5 µM 6.91E-6 60S ribosome export 15 2.83E-5
8 SGTC_2646 retinol 3.81 96.8 µM 1.95E-5 14 7.01E-5
9 SGTC_691 1548-0686 3.61 185.0 µM 4.84E-5 52 1.55E-4
10 SGTC_924 2046-0044 3.45 21.3 µM 9.46E-5 23 2.79E-4
11 SGTC_686 1319-0111 3.41 510.0 µM 1.12E-4 60S ribosome export 46 3.25E-4
12 SGTC_611 3453-1652 3.40 23.7 µM 1.19E-4 37 3.41E-4
13 SGTC_1573 oleylamine 3.39 1.1 µM 1.24E-4 51 3.54E-4
14 SGTC_2377 9073351 3.32 62.6 µM 1.61E-4 ergosterol biosynthesis 44 4.45E-4
15 SGTC_713 1488-1073 3.31 324.0 µM 1.73E-4 ERG2 27 4.74E-4
16 SGTC_151 3013-0144 3.29 2.3 µM 1.88E-4 iron homeostasis 54 5.09E-4
17 SGTC_1609 st001574 3.28 46.4 µM 1.91E-4 32 5.18E-4
18 SGTC_2547 epiafzelechin trimethyl ether 3.27 100.0 µM 2.00E-4 6 5.39E-4
19 SGTC_2209 5-(5-methyl-2-thenylidene)-Barbituric acid 3.16 122.8 µM 3.10E-4 26 7.89E-4
20 SGTC_1606 st000094 3.13 49.2 µM 3.44E-4 60S ribosome export 46 8.65E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.107 5.38E-10 YHR075C PPE1 hom Protein with carboxyl methyl esterase activity that may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein
0.097 1.80E-8 YDR134C YDR134C hom Hypothetical protein; YDR134C has a paralog, CCW12, that arose from the whole genome duplication
0.091 1.38E-7 YDR510W SMT3 het Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2
0.089 2.37E-7 YDR308C SRB7 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p
0.087 4.84E-7 YNL191W DUG3 hom Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p)
0.082 2.10E-6 YGR125W_p YGR125W_p hom Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
0.078 6.15E-6 YKR024C DBP7 hom Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions
0.073 2.37E-5 YPL090C RPS6A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication
0.071 3.67E-5 YKL071W_p YKL071W_p hom Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.070 4.91E-5 YLR061W RPL22A hom Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication
0.066 1.22E-4 YAL017W PSK1 hom PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication
0.064 1.92E-4 YNL329C PEX6 hom AAA-peroxin that heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol
0.064 2.18E-4 YNL303W_d YNL303W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.063 2.80E-4 YNL209W SSB2 hom Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; SSB2 has a paralog, SSB1, that arose from the whole genome duplication
0.062 3.35E-4 YER091C-A_d YER091C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data