YBR065C / ECM2

Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR065C / ECM2

Click on Significant Values for Screen Details ID:SGTC_164|Compound:4100-3994|FD-Score:3.04|P-value:8.92E-4 ID:SGTC_231|Compound:b-estradiol|FD-Score:-3.61|P-value:1.36E-4 ID:SGTC_244|Compound:nsc-207895|FD-Score:-4.14|P-value:1.50E-5 ID:SGTC_315|Compound:k915-0449|FD-Score:-3.63|P-value:1.26E-4 ID:SGTC_322|Compound:k048-0136|FD-Score:5.50|P-value:9.21E-9 ID:SGTC_329|Compound:k048-0121|FD-Score:-4.55|P-value:2.20E-6 ID:SGTC_332|Compound:4112-0506|FD-Score:-3.23|P-value:5.79E-4 ID:SGTC_337|Compound:1315-0038|FD-Score:-3.14|P-value:7.98E-4 ID:SGTC_417|Compound:st075190|FD-Score:4.02|P-value:1.90E-5 ID:SGTC_466|Compound:geldanamycin|FD-Score:4.69|P-value:7.68E-7 ID:SGTC_633|Compound:0437-0093|FD-Score:-5.53|P-value:1.10E-8 ID:SGTC_711|Compound:k292-0785|FD-Score:-3.90|P-value:4.18E-5 ID:SGTC_763|Compound:3453-1109|FD-Score:3.68|P-value:7.93E-5 ID:SGTC_768|Compound:0384-0033|FD-Score:3.55|P-value:1.37E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:-3.56|P-value:1.64E-4 ID:SGTC_808|Compound:0711-0032|FD-Score:4.06|P-value:1.60E-5 ID:SGTC_810|Compound:3825-7150|FD-Score:3.04|P-value:8.93E-4 ID:SGTC_817|Compound:1391-0715|FD-Score:5.32|P-value:2.59E-8 ID:SGTC_826|Compound:1683-5028|FD-Score:-4.00|P-value:2.77E-5 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.69|P-value:7.65E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.05|P-value:2.22E-5 ID:SGTC_1128|Compound:3448-9350|FD-Score:-3.75|P-value:7.91E-5 ID:SGTC_1138|Compound:3761-0006|FD-Score:3.15|P-value:6.16E-4 ID:SGTC_1212|Compound:0520-0165|FD-Score:4.74|P-value:5.92E-7 ID:SGTC_1291|Compound:0993-0025|FD-Score:4.18|P-value:9.33E-6 ID:SGTC_1306|Compound:1185-0270|FD-Score:4.90|P-value:2.69E-7 ID:SGTC_1540|Compound:guanosine|FD-Score:3.45|P-value:2.00E-4 ID:SGTC_1672|Compound:st016598|FD-Score:3.87|P-value:3.58E-5 ID:SGTC_1681|Compound:tripelennamine|FD-Score:-3.40|P-value:3.02E-4 ID:SGTC_1803|Compound:st050068|FD-Score:-3.37|P-value:3.43E-4 ID:SGTC_1820|Compound:st053765|FD-Score:5.13|P-value:7.52E-8 ID:SGTC_2050|Compound:5263056|FD-Score:3.31|P-value:3.38E-4 ID:SGTC_2054|Compound:5233173|FD-Score:4.13|P-value:1.15E-5 ID:SGTC_2270|Compound:7924575|FD-Score:-3.15|P-value:7.53E-4 ID:SGTC_2275|Compound:7947463|FD-Score:4.53|P-value:1.76E-6 ID:SGTC_2282|Compound:7944268|FD-Score:3.84|P-value:4.05E-5 ID:SGTC_2285|Compound:7945142|FD-Score:5.78|P-value:1.69E-9 ID:SGTC_2293|Compound:7624732|FD-Score:-4.95|P-value:2.83E-7 ID:SGTC_2381|Compound:9035592|FD-Score:-3.14|P-value:7.77E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:3.48|P-value:1.78E-4 ID:SGTC_2739|Compound:tacrine|FD-Score:3.19|P-value:5.27E-4 ID:SGTC_2761|Compound:levamisole|FD-Score:3.80|P-value:4.93E-5 ID:SGTC_2839|Compound:9003951|FD-Score:3.32|P-value:3.20E-4 ID:SGTC_2986|Compound:9022445|FD-Score:3.06|P-value:8.17E-4 ID:SGTC_3023|Compound:9089636|FD-Score:-3.10|P-value:8.90E-4 ID:SGTC_3037|Compound:9091026|FD-Score:-3.13|P-value:8.21E-4 ID:SGTC_3042|Compound:9091578|FD-Score:3.33|P-value:3.11E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:3.04|P-value:8.92E-4 ID:SGTC_231|Compound:b-estradiol|FD-Score:-3.61|P-value:1.36E-4 ID:SGTC_244|Compound:nsc-207895|FD-Score:-4.14|P-value:1.50E-5 ID:SGTC_315|Compound:k915-0449|FD-Score:-3.63|P-value:1.26E-4 ID:SGTC_322|Compound:k048-0136|FD-Score:5.50|P-value:9.21E-9 ID:SGTC_329|Compound:k048-0121|FD-Score:-4.55|P-value:2.20E-6 ID:SGTC_332|Compound:4112-0506|FD-Score:-3.23|P-value:5.79E-4 ID:SGTC_337|Compound:1315-0038|FD-Score:-3.14|P-value:7.98E-4 ID:SGTC_417|Compound:st075190|FD-Score:4.02|P-value:1.90E-5 ID:SGTC_466|Compound:geldanamycin|FD-Score:4.69|P-value:7.68E-7 ID:SGTC_633|Compound:0437-0093|FD-Score:-5.53|P-value:1.10E-8 ID:SGTC_711|Compound:k292-0785|FD-Score:-3.90|P-value:4.18E-5 ID:SGTC_763|Compound:3453-1109|FD-Score:3.68|P-value:7.93E-5 ID:SGTC_768|Compound:0384-0033|FD-Score:3.55|P-value:1.37E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:-3.56|P-value:1.64E-4 ID:SGTC_808|Compound:0711-0032|FD-Score:4.06|P-value:1.60E-5 ID:SGTC_810|Compound:3825-7150|FD-Score:3.04|P-value:8.93E-4 ID:SGTC_817|Compound:1391-0715|FD-Score:5.32|P-value:2.59E-8 ID:SGTC_826|Compound:1683-5028|FD-Score:-4.00|P-value:2.77E-5 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.69|P-value:7.65E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.05|P-value:2.22E-5 ID:SGTC_1128|Compound:3448-9350|FD-Score:-3.75|P-value:7.91E-5 ID:SGTC_1138|Compound:3761-0006|FD-Score:3.15|P-value:6.16E-4 ID:SGTC_1212|Compound:0520-0165|FD-Score:4.74|P-value:5.92E-7 ID:SGTC_1291|Compound:0993-0025|FD-Score:4.18|P-value:9.33E-6 ID:SGTC_1306|Compound:1185-0270|FD-Score:4.90|P-value:2.69E-7 ID:SGTC_1540|Compound:guanosine|FD-Score:3.45|P-value:2.00E-4 ID:SGTC_1672|Compound:st016598|FD-Score:3.87|P-value:3.58E-5 ID:SGTC_1681|Compound:tripelennamine|FD-Score:-3.40|P-value:3.02E-4 ID:SGTC_1803|Compound:st050068|FD-Score:-3.37|P-value:3.43E-4 ID:SGTC_1820|Compound:st053765|FD-Score:5.13|P-value:7.52E-8 ID:SGTC_2050|Compound:5263056|FD-Score:3.31|P-value:3.38E-4 ID:SGTC_2054|Compound:5233173|FD-Score:4.13|P-value:1.15E-5 ID:SGTC_2270|Compound:7924575|FD-Score:-3.15|P-value:7.53E-4 ID:SGTC_2275|Compound:7947463|FD-Score:4.53|P-value:1.76E-6 ID:SGTC_2282|Compound:7944268|FD-Score:3.84|P-value:4.05E-5 ID:SGTC_2285|Compound:7945142|FD-Score:5.78|P-value:1.69E-9 ID:SGTC_2293|Compound:7624732|FD-Score:-4.95|P-value:2.83E-7 ID:SGTC_2381|Compound:9035592|FD-Score:-3.14|P-value:7.77E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:3.48|P-value:1.78E-4 ID:SGTC_2739|Compound:tacrine|FD-Score:3.19|P-value:5.27E-4 ID:SGTC_2761|Compound:levamisole|FD-Score:3.80|P-value:4.93E-5 ID:SGTC_2839|Compound:9003951|FD-Score:3.32|P-value:3.20E-4 ID:SGTC_2986|Compound:9022445|FD-Score:3.06|P-value:8.17E-4 ID:SGTC_3023|Compound:9089636|FD-Score:-3.10|P-value:8.90E-4 ID:SGTC_3037|Compound:9091026|FD-Score:-3.13|P-value:8.21E-4 ID:SGTC_3042|Compound:9091578|FD-Score:3.33|P-value:3.11E-4

Top fitness defect scores for YBR065C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2285 7945142 5.78 200.0 µM 1.69E-9 4 3.70E-9
2 SGTC_322 k048-0136 5.50 19.3 µM 9.21E-9 RSC & ERG11 3 1.90E-8
3 SGTC_817 1391-0715 5.32 46.0 µM 2.59E-8 2 5.13E-8
4 SGTC_1820 st053765 5.13 18.6 µM 7.52E-8 RPP1 & pyrimidine depletion 2 1.43E-7
5 SGTC_1306 1185-0270 4.90 9.0 µM 2.69E-7 Golgi 42 4.89E-7
6 SGTC_1212 0520-0165 4.74 585.0 µM 5.92E-7 12 1.04E-6
7 SGTC_466 geldanamycin 4.69 89.2 µM 7.68E-7 RPP1 & pyrimidine depletion 3 1.34E-6
8 SGTC_2275 7947463 4.53 200.0 µM 1.76E-6 iron homeostasis 27 2.97E-6
9 SGTC_1291 0993-0025 4.18 5.8 µM 9.33E-6 9 1.48E-5
10 SGTC_2054 5233173 4.13 129.0 µM 1.15E-5 mitochondrial response to ROS 48 1.80E-5
11 SGTC_808 0711-0032 4.06 135.0 µM 1.60E-5 10 2.48E-5
12 SGTC_417 st075190 4.02 100.0 µM 1.90E-5 7 2.93E-5
13 SGTC_1672 st016598 3.87 6.5 µM 3.58E-5 endomembrane recycling 69 5.38E-5
14 SGTC_2282 7944268 3.84 200.0 µM 4.05E-5 RPP1 & pyrimidine depletion 33 6.06E-5
15 SGTC_2761 levamisole 3.80 97.9 µM 4.93E-5 18 7.33E-5
16 SGTC_1004 4084-0029 3.69 199.0 µM 7.65E-5 23 1.12E-4
17 SGTC_763 3453-1109 3.68 128.0 µM 7.93E-5 25 1.16E-4
18 SGTC_768 0384-0033 3.55 77.3 µM 1.37E-4 fatty acid desaturase (OLE1) 101 1.96E-4
19 SGTC_2690 usnic acid 3.48 100.0 µM 1.78E-4 46 2.51E-4
20 SGTC_1540 guanosine 3.45 70.6 µM 2.00E-4 16 2.81E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.151 1.52E-18 YOL141W PPM2 hom AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p
0.128 8.14E-14 YML074C FPR3 hom Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p
0.118 7.75E-12 YDR246W-A_p YDR246W-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.112 8.16E-11 YDR139C RUB1 hom Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme)
0.112 8.69E-11 YLR269C_d YLR269C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.107 6.02E-10 YDR110W FOB1 hom Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases
0.104 1.36E-9 YAL045C_d YAL045C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A
0.102 3.31E-9 YGL017W ATE1 hom Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway
0.101 3.89E-9 YJL058C BIT61 hom Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication
0.101 5.21E-9 YLR326W_p YLR326W_p hom Putative protein of unknown function, predicted to be palmitoylated
0.096 2.37E-8 YGR138C TPO2 hom Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; TPO2 has a paralog, TPO3, that arose from the whole genome duplication
0.092 8.46E-8 YLR205C HMX1 hom ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
0.092 8.99E-8 YDL110C TMA17 hom Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion
0.091 1.22E-7 YCR102W-A_d YCR102W-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.090 1.56E-7 YDR210W_p YDR210W_p hom Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery