YBR115C / LYS2

Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR115C / LYS2

Click on Significant Values for Screen Details ID:SGTC_56|Compound:0957-0365|FD-Score:5.03|P-value:3.65E-8 ID:SGTC_57|Compound:miconazole|FD-Score:3.18|P-value:3.23E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:3.08|P-value:4.76E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:-4.43|P-value:1.07E-6 ID:SGTC_149|Compound:3970-0822|FD-Score:-5.25|P-value:9.77E-9 ID:SGTC_182|Compound:3448-4371|FD-Score:4.43|P-value:1.07E-6 ID:SGTC_382|Compound:3229-0966|FD-Score:3.05|P-value:5.52E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.36|P-value:1.61E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:3.39|P-value:1.41E-4 ID:SGTC_432|Compound:NS 1619|FD-Score:-5.04|P-value:3.46E-8 ID:SGTC_438|Compound:sphingosine|FD-Score:5.09|P-value:2.57E-8 ID:SGTC_622|Compound:1348-1566|FD-Score:3.84|P-value:1.92E-5 ID:SGTC_762|Compound:1165-0429|FD-Score:2.89|P-value:9.98E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:3.01|P-value:6.34E-4 ID:SGTC_828|Compound:2074-0507|FD-Score:4.18|P-value:3.76E-6 ID:SGTC_901|Compound:1112-0062|FD-Score:-3.69|P-value:3.97E-5 ID:SGTC_912|Compound:3474-0017|FD-Score:3.34|P-value:1.74E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:3.22|P-value:2.83E-4 ID:SGTC_1039|Compound:k821-0043|FD-Score:3.54|P-value:7.39E-5 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.65|P-value:4.71E-5 ID:SGTC_1205|Compound:4161-2785|FD-Score:-3.42|P-value:1.28E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.33|P-value:1.83E-4 ID:SGTC_1521|Compound:st012942|FD-Score:4.04|P-value:7.39E-6 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:3.60|P-value:5.65E-5 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:5.15|P-value:1.76E-8 ID:SGTC_1654|Compound:st012436|FD-Score:3.15|P-value:3.71E-4 ID:SGTC_1675|Compound:st018485|FD-Score:-3.39|P-value:1.46E-4 ID:SGTC_1690|Compound:st023502|FD-Score:3.42|P-value:1.23E-4 ID:SGTC_1872|Compound:amphotericin b|FD-Score:3.05|P-value:5.37E-4 ID:SGTC_1948|Compound:st076329|FD-Score:4.72|P-value:2.13E-7 ID:SGTC_2242|Compound:6630449|FD-Score:-3.01|P-value:6.51E-4 ID:SGTC_2360|Compound:9040895|FD-Score:-3.62|P-value:5.38E-5 ID:SGTC_2365|Compound:9042475|FD-Score:-3.12|P-value:4.28E-4 ID:SGTC_2403|Compound:6032726|FD-Score:-4.81|P-value:1.33E-7 ID:SGTC_2477|Compound:5967686|FD-Score:-4.57|P-value:4.91E-7 ID:SGTC_2512|Compound:diallyl trisulfide|FD-Score:-3.68|P-value:4.05E-5 ID:SGTC_2513|Compound:baicalein|FD-Score:-4.35|P-value:1.63E-6 ID:SGTC_2523|Compound:spermine|FD-Score:3.24|P-value:2.64E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:4.60|P-value:4.18E-7 ID:SGTC_2830|Compound:7992007|FD-Score:-2.96|P-value:7.65E-4 ID:SGTC_2848|Compound:9016370|FD-Score:3.09|P-value:4.62E-4 ID:SGTC_3206|Compound:9114350|FD-Score:-8.96|P-value:4.53E-22 ID:SGTC_3207|Compound:9114163|FD-Score:-4.54|P-value:5.94E-7 ID:SGTC_3208|Compound:9114115|FD-Score:-4.78|P-value:1.60E-7 ID:SGTC_56|Compound:0957-0365|FD-Score:5.03|P-value:3.65E-8 ID:SGTC_57|Compound:miconazole|FD-Score:3.18|P-value:3.23E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:3.08|P-value:4.76E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:-4.43|P-value:1.07E-6 ID:SGTC_149|Compound:3970-0822|FD-Score:-5.25|P-value:9.77E-9 ID:SGTC_182|Compound:3448-4371|FD-Score:4.43|P-value:1.07E-6 ID:SGTC_382|Compound:3229-0966|FD-Score:3.05|P-value:5.52E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.36|P-value:1.61E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:3.39|P-value:1.41E-4 ID:SGTC_432|Compound:NS 1619|FD-Score:-5.04|P-value:3.46E-8 ID:SGTC_438|Compound:sphingosine|FD-Score:5.09|P-value:2.57E-8 ID:SGTC_622|Compound:1348-1566|FD-Score:3.84|P-value:1.92E-5 ID:SGTC_762|Compound:1165-0429|FD-Score:2.89|P-value:9.98E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:3.01|P-value:6.34E-4 ID:SGTC_828|Compound:2074-0507|FD-Score:4.18|P-value:3.76E-6 ID:SGTC_901|Compound:1112-0062|FD-Score:-3.69|P-value:3.97E-5 ID:SGTC_912|Compound:3474-0017|FD-Score:3.34|P-value:1.74E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:3.22|P-value:2.83E-4 ID:SGTC_1039|Compound:k821-0043|FD-Score:3.54|P-value:7.39E-5 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.65|P-value:4.71E-5 ID:SGTC_1205|Compound:4161-2785|FD-Score:-3.42|P-value:1.28E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.33|P-value:1.83E-4 ID:SGTC_1521|Compound:st012942|FD-Score:4.04|P-value:7.39E-6 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:3.60|P-value:5.65E-5 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:5.15|P-value:1.76E-8 ID:SGTC_1654|Compound:st012436|FD-Score:3.15|P-value:3.71E-4 ID:SGTC_1675|Compound:st018485|FD-Score:-3.39|P-value:1.46E-4 ID:SGTC_1690|Compound:st023502|FD-Score:3.42|P-value:1.23E-4 ID:SGTC_1872|Compound:amphotericin b|FD-Score:3.05|P-value:5.37E-4 ID:SGTC_1948|Compound:st076329|FD-Score:4.72|P-value:2.13E-7 ID:SGTC_2242|Compound:6630449|FD-Score:-3.01|P-value:6.51E-4 ID:SGTC_2360|Compound:9040895|FD-Score:-3.62|P-value:5.38E-5 ID:SGTC_2365|Compound:9042475|FD-Score:-3.12|P-value:4.28E-4 ID:SGTC_2403|Compound:6032726|FD-Score:-4.81|P-value:1.33E-7 ID:SGTC_2477|Compound:5967686|FD-Score:-4.57|P-value:4.91E-7 ID:SGTC_2512|Compound:diallyl trisulfide|FD-Score:-3.68|P-value:4.05E-5 ID:SGTC_2513|Compound:baicalein|FD-Score:-4.35|P-value:1.63E-6 ID:SGTC_2523|Compound:spermine|FD-Score:3.24|P-value:2.64E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:4.60|P-value:4.18E-7 ID:SGTC_2830|Compound:7992007|FD-Score:-2.96|P-value:7.65E-4 ID:SGTC_2848|Compound:9016370|FD-Score:3.09|P-value:4.62E-4 ID:SGTC_3206|Compound:9114350|FD-Score:-8.96|P-value:4.53E-22 ID:SGTC_3207|Compound:9114163|FD-Score:-4.54|P-value:5.94E-7 ID:SGTC_3208|Compound:9114115|FD-Score:-4.78|P-value:1.60E-7

Top fitness defect scores for YBR115C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1557 sumaresinolic acid 5.15 42.3 µM 1.76E-8 5 1.32E-7
2 SGTC_438 sphingosine 5.09 6.7 µM 2.57E-8 9 1.83E-7
3 SGTC_56 0957-0365 5.03 83.2 µM 3.65E-8 Golgi 27 2.49E-7
4 SGTC_1948 st076329 4.72 66.6 µM 2.13E-7 13 1.17E-6
5 SGTC_2533 agelasine 4.60 13.7 µM 4.18E-7 ERG2 32 2.11E-6
6 SGTC_182 3448-4371 4.43 550.5 µM 1.07E-6 16 4.81E-6
7 SGTC_828 2074-0507 4.18 139.0 µM 3.76E-6 RPP1 & pyrimidine depletion 20 1.45E-5
8 SGTC_1521 st012942 4.04 49.4 µM 7.39E-6 29 2.62E-5
9 SGTC_622 1348-1566 3.84 26.3 µM 1.92E-5 21 6.05E-5
10 SGTC_1043 0916-0009 3.65 19.3 µM 4.71E-5 31 1.33E-4
11 SGTC_1525 8-methoxypsoralen 3.60 92.5 µM 5.65E-5 DNA damage response 43 1.56E-4
12 SGTC_1039 k821-0043 3.54 94.0 µM 7.39E-5 Golgi 43 1.98E-4
13 SGTC_1690 st023502 3.42 41.2 µM 1.23E-4 calcium & mitochondrial duress 21 3.10E-4
14 SGTC_426 0988-0037 3.39 14.6 µM 1.41E-4 ERAD & cell cycle 58 3.49E-4
15 SGTC_421 streptovitacin 3.36 30.0 mM 1.61E-4 48 3.92E-4
16 SGTC_912 3474-0017 3.34 2.2 µM 1.74E-4 43 4.19E-4
17 SGTC_2523 spermine 3.24 47.7 µM 2.64E-4 calcium & mitochondrial duress 48 6.06E-4
18 SGTC_916 4466-0038 3.22 42.0 nM 2.83E-4 cell wall 116 6.44E-4
19 SGTC_57 miconazole 3.18 162.5 nM 3.23E-4 ergosterol depletion effects on membrane 49 7.25E-4
20 SGTC_1654 st012436 3.15 41.8 µM 3.71E-4 37 8.18E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.181 5.43E-26 YBL043W ECM13 hom Non-essential protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; ECM13 has a paralog, YJR115W, that arose from the whole genome duplication
0.178 2.29E-25 YBL015W ACH1 hom Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth
0.176 9.15E-25 YDR294C DPL1 hom Dihydrosphingosine phosphate lyase, regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate
0.165 6.64E-22 YLR189C ATG26 hom UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy
0.160 1.20E-20 YHL016C DUR3 hom Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway
0.154 3.11E-19 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.137 1.28E-15 YJR011C_p YJR011C_p hom Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS
0.130 3.95E-14 YBR165W UBS1 hom Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity
0.130 4.82E-14 YOR266W PNT1 hom Mitochondrial integral inner membrane protein involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; deletion mutant sensitive to the anti-Pneumocystis carinii drug pentamidine
0.122 1.28E-12 YJR002W MPP10 het Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p
0.113 5.41E-11 YDR355C_d YDR355C_d het Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W
0.111 1.38E-10 YOR152C_p YOR152C_p hom Putative protein of unknown function; YOR152C is not an essential gene
0.107 4.53E-10 YDR193W_d YDR193W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.106 8.49E-10 YML119W_p YML119W_p hom Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate
0.105 9.28E-10 YGR001C_p YGR001C_p hom Putative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae