YBR125C / PTC4

Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR125C / PTC4

Click on Significant Values for Screen Details ID:SGTC_103|Compound:0844-0104|FD-Score:3.03|P-value:3.73E-4 ID:SGTC_156|Compound:3474-0145|FD-Score:2.79|P-value:9.46E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:-4.45|P-value:4.38E-7 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.29|P-value:1.30E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:3.52|P-value:4.62E-5 ID:SGTC_643|Compound:k048-0106|FD-Score:2.82|P-value:8.63E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:3.25|P-value:1.54E-4 ID:SGTC_948|Compound:3448-0295|FD-Score:-3.25|P-value:1.66E-4 ID:SGTC_1215|Compound:3852-0577|FD-Score:-2.86|P-value:8.02E-4 ID:SGTC_1252|Compound:0493-0875|FD-Score:-3.17|P-value:2.39E-4 ID:SGTC_1563|Compound:conessine|FD-Score:2.96|P-value:5.05E-4 ID:SGTC_1745|Compound:st081495|FD-Score:2.99|P-value:4.50E-4 ID:SGTC_1864|Compound:st057645|FD-Score:-3.52|P-value:5.19E-5 ID:SGTC_1865|Compound:st057639|FD-Score:-3.38|P-value:9.73E-5 ID:SGTC_1873|Compound:rapamycin|FD-Score:-3.88|P-value:9.31E-6 ID:SGTC_1876|Compound:myriocin|FD-Score:11.40|P-value:4.05E-37 ID:SGTC_2050|Compound:5263056|FD-Score:3.58|P-value:3.52E-5 ID:SGTC_2427|Compound:5545055|FD-Score:4.04|P-value:3.58E-6 ID:SGTC_2537|Compound:larixol|FD-Score:3.08|P-value:3.07E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:2.83|P-value:8.13E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:2.94|P-value:5.41E-4 ID:SGTC_2605|Compound:digitonin|FD-Score:4.62|P-value:1.42E-7 ID:SGTC_2608|Compound:thymoquinone|FD-Score:5.07|P-value:9.18E-9 ID:SGTC_2693|Compound:st077581|FD-Score:4.27|P-value:1.04E-6 ID:SGTC_2708|Compound:st078559|FD-Score:3.12|P-value:2.59E-4 ID:SGTC_2714|Compound:st079313|FD-Score:-3.38|P-value:9.48E-5 ID:SGTC_2750|Compound:bromebric acid|FD-Score:3.24|P-value:1.58E-4 ID:SGTC_2761|Compound:levamisole|FD-Score:3.62|P-value:2.89E-5 ID:SGTC_2770|Compound:toltrazuril|FD-Score:3.85|P-value:9.48E-6 ID:SGTC_2800|Compound:7887935|FD-Score:4.19|P-value:1.59E-6 ID:SGTC_2849|Compound:9019321|FD-Score:5.09|P-value:8.24E-9 ID:SGTC_2866|Compound:9034245|FD-Score:3.25|P-value:1.54E-4 ID:SGTC_2876|Compound:9059903|FD-Score:3.87|P-value:8.58E-6 ID:SGTC_2898|Compound:9056270|FD-Score:3.12|P-value:2.62E-4 ID:SGTC_2978|Compound:9011784|FD-Score:2.81|P-value:8.73E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:3.03|P-value:3.73E-4 ID:SGTC_156|Compound:3474-0145|FD-Score:2.79|P-value:9.46E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:-4.45|P-value:4.38E-7 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.29|P-value:1.30E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:3.52|P-value:4.62E-5 ID:SGTC_643|Compound:k048-0106|FD-Score:2.82|P-value:8.63E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:3.25|P-value:1.54E-4 ID:SGTC_948|Compound:3448-0295|FD-Score:-3.25|P-value:1.66E-4 ID:SGTC_1215|Compound:3852-0577|FD-Score:-2.86|P-value:8.02E-4 ID:SGTC_1252|Compound:0493-0875|FD-Score:-3.17|P-value:2.39E-4 ID:SGTC_1563|Compound:conessine|FD-Score:2.96|P-value:5.05E-4 ID:SGTC_1745|Compound:st081495|FD-Score:2.99|P-value:4.50E-4 ID:SGTC_1864|Compound:st057645|FD-Score:-3.52|P-value:5.19E-5 ID:SGTC_1865|Compound:st057639|FD-Score:-3.38|P-value:9.73E-5 ID:SGTC_1873|Compound:rapamycin|FD-Score:-3.88|P-value:9.31E-6 ID:SGTC_1876|Compound:myriocin|FD-Score:11.40|P-value:4.05E-37 ID:SGTC_2050|Compound:5263056|FD-Score:3.58|P-value:3.52E-5 ID:SGTC_2427|Compound:5545055|FD-Score:4.04|P-value:3.58E-6 ID:SGTC_2537|Compound:larixol|FD-Score:3.08|P-value:3.07E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:2.83|P-value:8.13E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:2.94|P-value:5.41E-4 ID:SGTC_2605|Compound:digitonin|FD-Score:4.62|P-value:1.42E-7 ID:SGTC_2608|Compound:thymoquinone|FD-Score:5.07|P-value:9.18E-9 ID:SGTC_2693|Compound:st077581|FD-Score:4.27|P-value:1.04E-6 ID:SGTC_2708|Compound:st078559|FD-Score:3.12|P-value:2.59E-4 ID:SGTC_2714|Compound:st079313|FD-Score:-3.38|P-value:9.48E-5 ID:SGTC_2750|Compound:bromebric acid|FD-Score:3.24|P-value:1.58E-4 ID:SGTC_2761|Compound:levamisole|FD-Score:3.62|P-value:2.89E-5 ID:SGTC_2770|Compound:toltrazuril|FD-Score:3.85|P-value:9.48E-6 ID:SGTC_2800|Compound:7887935|FD-Score:4.19|P-value:1.59E-6 ID:SGTC_2849|Compound:9019321|FD-Score:5.09|P-value:8.24E-9 ID:SGTC_2866|Compound:9034245|FD-Score:3.25|P-value:1.54E-4 ID:SGTC_2876|Compound:9059903|FD-Score:3.87|P-value:8.58E-6 ID:SGTC_2898|Compound:9056270|FD-Score:3.12|P-value:2.62E-4 ID:SGTC_2978|Compound:9011784|FD-Score:2.81|P-value:8.73E-4

Top fitness defect scores for YBR125C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1876 myriocin 11.40 605.0 nM 4.05E-37 5 1.38E-30
2 SGTC_2849 9019321 5.09 13.0 µM 8.24E-9 14 1.83E-7
3 SGTC_2608 thymoquinone 5.07 42.7 µM 9.18E-9 8 2.00E-7
4 SGTC_2605 digitonin 4.62 300.0 nM 1.42E-7 12 1.88E-6
5 SGTC_2693 st077581 4.27 40.7 µM 1.04E-6 25 9.61E-6
6 SGTC_2800 7887935 4.19 81.8 µM 1.59E-6 RPP1 & pyrimidine depletion 27 1.37E-5
7 SGTC_2427 5545055 4.04 42.8 µM 3.58E-6 11 2.65E-5
8 SGTC_2876 9059903 3.87 26.0 µM 8.58E-6 14 5.44E-5
9 SGTC_2770 toltrazuril 3.85 17.5 µM 9.48E-6 32 5.90E-5
10 SGTC_2761 levamisole 3.62 97.9 µM 2.89E-5 23 1.47E-4
11 SGTC_2050 5263056 3.58 171.0 µM 3.52E-5 fatty acid desaturase (OLE1) 58 1.73E-4
12 SGTC_604 1000-0114 3.52 314.0 µM 4.62E-5 38 2.17E-4
13 SGTC_445 e6 berbamine 3.29 66.0 µM 1.30E-4 NEO1-PIK1 164 5.09E-4
14 SGTC_873 0591-4978 3.25 488.0 µM 1.54E-4 37 5.84E-4
15 SGTC_2866 9034245 3.25 58.4 µM 1.54E-4 21 5.85E-4
16 SGTC_2750 bromebric acid 3.24 70.2 µM 1.58E-4 65 5.95E-4
17 SGTC_2708 st078559 3.12 56.0 µM 2.59E-4 25 8.98E-4
18 SGTC_2898 9056270 3.12 58.4 µM 2.62E-4 18 9.06E-4
19 SGTC_2537 larixol 3.08 68.9 µM 3.07E-4 64 0.00103
20 SGTC_103 0844-0104 3.03 51.8 µM 3.73E-4 ERAD & cell cycle 204 0.00121

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.474 2.03E-187 YGR212W SLI1 hom N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin
0.340 1.80E-91 YMR296C LCB1 het Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
0.258 2.74E-52 YDR071C PAA1 hom Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication
0.211 3.86E-35 YGR213C RTA1 hom Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein
0.177 4.46E-25 YNL129W NRK1 hom Nicotinamide riboside kinase, catalyzes the phosphorylation of nicotinamide riboside and nicotinic acid riboside in salvage pathways for NAD+ biosynthesis
0.158 2.79E-20 YDR094W_d YDR094W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2
0.155 2.17E-19 YDR191W HST4 hom Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism
0.150 4.18E-18 YHR153C SPO16 hom Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation
0.149 3.29E-18 YJL213W YJL213W hom Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p
0.139 6.59E-16 YHR041C SRB2 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance
0.137 1.48E-15 YBR207W FTH1 hom Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress
0.134 7.95E-15 YDR062W LCB2 het Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
0.129 6.18E-14 YJR127C RSF2 hom Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.122 1.19E-12 YHR046C INM1 hom Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate
0.121 4.93E-12 YKR099W BAS1 hom Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes