YBR128C / ATG14

Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I (with Vps34/15/30p); Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR128C / ATG14

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:3.53|P-value:1.48E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:-3.07|P-value:8.39E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:3.65|P-value:8.88E-5 ID:SGTC_486|Compound:niguldipine|FD-Score:3.56|P-value:1.32E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:3.30|P-value:3.51E-4 ID:SGTC_982|Compound:1491-0518|FD-Score:3.46|P-value:1.94E-4 ID:SGTC_1031|Compound:k035-0009|FD-Score:3.16|P-value:5.94E-4 ID:SGTC_1295|Compound:1013-0063|FD-Score:3.39|P-value:2.48E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:4.41|P-value:2.96E-6 ID:SGTC_1464|Compound:k208-0027|FD-Score:3.58|P-value:1.18E-4 ID:SGTC_1471|Compound:k673-0312|FD-Score:3.27|P-value:3.88E-4 ID:SGTC_1475|Compound:4092-1048|FD-Score:5.17|P-value:5.57E-8 ID:SGTC_1523|Compound:st012844|FD-Score:3.03|P-value:9.21E-4 ID:SGTC_1600|Compound:coumarin 106|FD-Score:3.24|P-value:4.44E-4 ID:SGTC_1603|Compound:st002045|FD-Score:-3.39|P-value:2.66E-4 ID:SGTC_1604|Compound:st002052|FD-Score:3.31|P-value:3.44E-4 ID:SGTC_1617|Compound:st002906|FD-Score:-3.45|P-value:2.03E-4 ID:SGTC_1619|Compound:st003326|FD-Score:-3.67|P-value:8.50E-5 ID:SGTC_1762|Compound:st045503|FD-Score:3.02|P-value:9.56E-4 ID:SGTC_1765|Compound:st044815|FD-Score:3.52|P-value:1.50E-4 ID:SGTC_1823|Compound:3-hydroxy-2-naphthoic acid|FD-Score:3.80|P-value:4.78E-5 ID:SGTC_1933|Compound:st074711|FD-Score:4.81|P-value:4.01E-7 ID:SGTC_1935|Compound:st074532|FD-Score:3.73|P-value:6.55E-5 ID:SGTC_1962|Compound:st076595|FD-Score:3.10|P-value:7.34E-4 ID:SGTC_1983|Compound:st071813|FD-Score:3.27|P-value:3.95E-4 ID:SGTC_1997|Compound:st073024|FD-Score:3.43|P-value:2.12E-4 ID:SGTC_2031|Compound:5180455|FD-Score:-3.22|P-value:4.83E-4 ID:SGTC_2079|Compound:5212404|FD-Score:-3.24|P-value:4.62E-4 ID:SGTC_2115|Compound:tiliquinol|FD-Score:4.67|P-value:8.36E-7 ID:SGTC_2300|Compound:7976697|FD-Score:-3.11|P-value:7.38E-4 ID:SGTC_2434|Compound:5670830|FD-Score:-3.50|P-value:1.68E-4 ID:SGTC_2663|Compound:amphotericin b|FD-Score:4.87|P-value:2.95E-7 ID:SGTC_2682|Compound:pyrogallin|FD-Score:-3.21|P-value:5.04E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-3.08|P-value:8.05E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:-3.98|P-value:2.32E-5 ID:SGTC_2827|Compound:7993847|FD-Score:3.11|P-value:7.05E-4 ID:SGTC_2860|Compound:9025725|FD-Score:3.38|P-value:2.62E-4 ID:SGTC_2970|Compound:9090938|FD-Score:3.75|P-value:5.86E-5 ID:SGTC_2975|Compound:9084847|FD-Score:4.36|P-value:3.87E-6 ID:SGTC_3007|Compound:9078383|FD-Score:3.78|P-value:5.16E-5 ID:SGTC_3026|Compound:9090633|FD-Score:3.08|P-value:7.84E-4 ID:SGTC_3071|Compound:9117417|FD-Score:3.08|P-value:7.90E-4 ID:SGTC_3086|Compound:9117350|FD-Score:3.27|P-value:3.94E-4 ID:SGTC_3202|Compound:9113761|FD-Score:4.15|P-value:1.02E-5 ID:SGTC_3209|Compound:9113616|FD-Score:4.51|P-value:1.84E-6 ID:SGTC_3210|Compound:9128157|FD-Score:-3.30|P-value:3.72E-4 ID:SGTC_3242|Compound:9133940|FD-Score:3.05|P-value:8.69E-4 ID:SGTC_3302|Compound:9125395|FD-Score:-3.44|P-value:2.14E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:3.53|P-value:1.48E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:-3.07|P-value:8.39E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:3.65|P-value:8.88E-5 ID:SGTC_486|Compound:niguldipine|FD-Score:3.56|P-value:1.32E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:3.30|P-value:3.51E-4 ID:SGTC_982|Compound:1491-0518|FD-Score:3.46|P-value:1.94E-4 ID:SGTC_1031|Compound:k035-0009|FD-Score:3.16|P-value:5.94E-4 ID:SGTC_1295|Compound:1013-0063|FD-Score:3.39|P-value:2.48E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:4.41|P-value:2.96E-6 ID:SGTC_1464|Compound:k208-0027|FD-Score:3.58|P-value:1.18E-4 ID:SGTC_1471|Compound:k673-0312|FD-Score:3.27|P-value:3.88E-4 ID:SGTC_1475|Compound:4092-1048|FD-Score:5.17|P-value:5.57E-8 ID:SGTC_1523|Compound:st012844|FD-Score:3.03|P-value:9.21E-4 ID:SGTC_1600|Compound:coumarin 106|FD-Score:3.24|P-value:4.44E-4 ID:SGTC_1603|Compound:st002045|FD-Score:-3.39|P-value:2.66E-4 ID:SGTC_1604|Compound:st002052|FD-Score:3.31|P-value:3.44E-4 ID:SGTC_1617|Compound:st002906|FD-Score:-3.45|P-value:2.03E-4 ID:SGTC_1619|Compound:st003326|FD-Score:-3.67|P-value:8.50E-5 ID:SGTC_1762|Compound:st045503|FD-Score:3.02|P-value:9.56E-4 ID:SGTC_1765|Compound:st044815|FD-Score:3.52|P-value:1.50E-4 ID:SGTC_1823|Compound:3-hydroxy-2-naphthoic acid|FD-Score:3.80|P-value:4.78E-5 ID:SGTC_1933|Compound:st074711|FD-Score:4.81|P-value:4.01E-7 ID:SGTC_1935|Compound:st074532|FD-Score:3.73|P-value:6.55E-5 ID:SGTC_1962|Compound:st076595|FD-Score:3.10|P-value:7.34E-4 ID:SGTC_1983|Compound:st071813|FD-Score:3.27|P-value:3.95E-4 ID:SGTC_1997|Compound:st073024|FD-Score:3.43|P-value:2.12E-4 ID:SGTC_2031|Compound:5180455|FD-Score:-3.22|P-value:4.83E-4 ID:SGTC_2079|Compound:5212404|FD-Score:-3.24|P-value:4.62E-4 ID:SGTC_2115|Compound:tiliquinol|FD-Score:4.67|P-value:8.36E-7 ID:SGTC_2300|Compound:7976697|FD-Score:-3.11|P-value:7.38E-4 ID:SGTC_2434|Compound:5670830|FD-Score:-3.50|P-value:1.68E-4 ID:SGTC_2663|Compound:amphotericin b|FD-Score:4.87|P-value:2.95E-7 ID:SGTC_2682|Compound:pyrogallin|FD-Score:-3.21|P-value:5.04E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-3.08|P-value:8.05E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:-3.98|P-value:2.32E-5 ID:SGTC_2827|Compound:7993847|FD-Score:3.11|P-value:7.05E-4 ID:SGTC_2860|Compound:9025725|FD-Score:3.38|P-value:2.62E-4 ID:SGTC_2970|Compound:9090938|FD-Score:3.75|P-value:5.86E-5 ID:SGTC_2975|Compound:9084847|FD-Score:4.36|P-value:3.87E-6 ID:SGTC_3007|Compound:9078383|FD-Score:3.78|P-value:5.16E-5 ID:SGTC_3026|Compound:9090633|FD-Score:3.08|P-value:7.84E-4 ID:SGTC_3071|Compound:9117417|FD-Score:3.08|P-value:7.90E-4 ID:SGTC_3086|Compound:9117350|FD-Score:3.27|P-value:3.94E-4 ID:SGTC_3202|Compound:9113761|FD-Score:4.15|P-value:1.02E-5 ID:SGTC_3209|Compound:9113616|FD-Score:4.51|P-value:1.84E-6 ID:SGTC_3210|Compound:9128157|FD-Score:-3.30|P-value:3.72E-4 ID:SGTC_3242|Compound:9133940|FD-Score:3.05|P-value:8.69E-4 ID:SGTC_3302|Compound:9125395|FD-Score:-3.44|P-value:2.14E-4

Top fitness defect scores for YBR128C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1475 4092-1048 5.17 11.7 µM 5.57E-8 plasma membrane duress 16 1.15E-7
2 SGTC_2663 amphotericin b 4.87 100.0 µM 2.95E-7 4 5.64E-7
3 SGTC_1933 st074711 4.81 15.9 µM 4.01E-7 22 7.55E-7
4 SGTC_2115 tiliquinol 4.67 12.8 µM 8.36E-7 3 1.52E-6
5 SGTC_3209 9113616 4.51 49.5 µM 1.84E-6 14 3.22E-6
6 SGTC_1457 k015-0032 4.41 57.3 µM 2.96E-6 25 5.07E-6
7 SGTC_2975 9084847 4.36 53.7 µM 3.87E-6 16 6.55E-6
8 SGTC_3202 9113761 4.15 49.5 µM 1.02E-5 41 1.64E-5
9 SGTC_1823 3-hydroxy-2-naphthoic acid 3.80 106.0 µM 4.78E-5 21 7.19E-5
10 SGTC_3007 9078383 3.78 71.4 µM 5.16E-5 19 7.73E-5
11 SGTC_2970 9090938 3.75 41.9 µM 5.86E-5 RNA processing & uracil transport 45 8.73E-5
12 SGTC_1935 st074532 3.73 41.6 µM 6.55E-5 40 9.71E-5
13 SGTC_430 0958-0115 3.65 16.4 µM 8.88E-5 34 1.30E-4
14 SGTC_1464 k208-0027 3.58 110.0 µM 1.18E-4 35 1.70E-4
15 SGTC_486 niguldipine 3.56 82.0 µM 1.32E-4 amide catabolism 24 1.89E-4
16 SGTC_64 000l-0018 3.53 272.1 µM 1.48E-4 ubiquinone biosynthesis & proteasome 177 2.11E-4
17 SGTC_1765 st044815 3.52 90.4 µM 1.50E-4 38 2.14E-4
18 SGTC_982 1491-0518 3.46 27.5 µM 1.94E-4 27 2.73E-4
19 SGTC_1997 st073024 3.43 93.8 µM 2.12E-4 17 2.97E-4
20 SGTC_1295 1013-0063 3.39 9.8 µM 2.48E-4 31 3.45E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.094 4.26E-8 YOR291W YPK9 hom Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome
0.093 7.38E-8 YOR054C VHS3 hom Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis
0.090 1.74E-7 YBL091C MAP2 hom Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p
0.089 2.57E-7 YBL068W PRS4 hom 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication
0.085 7.06E-7 YGL089C MF(ALPHA)2 hom Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)1, which is more highly expressed than MF(ALPHA)2
0.084 1.22E-6 YHL017W_p YHL017W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication
0.083 1.51E-6 YLR017W MEU1 hom Methylthioadenosine phosphorylase (MTAP), catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression
0.081 2.93E-6 YOR105W_p YOR105W_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.079 4.56E-6 YKL015W PUT3 hom Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain
0.077 8.46E-6 YBR054W YRO2 hom Protein of unknown function with similarity to archaeal rhodopsins; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; transcriptionally regulated by Haa1p; YRO2 has a paralog, MRH1, that arose from the whole genome duplication
0.075 1.51E-5 YLR445W GMC2 hom Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor
0.075 1.55E-5 YKL012W PRP40 het U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex
0.074 1.93E-5 YPL261C_d YPL261C_d hom Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W
0.073 2.60E-5 YPL263C KEL3 hom Cytoplasmic protein of unknown function
0.071 4.00E-5 YMR022W UBC7 hom Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly