HIPHOP chemogenomics database

YBR134W_d

Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Zygosity: Homozygous strain

fixedexpanded
Profile for YBR134W / YBR134W

Click on Significant Values for Screen Details

Top fitness defect scores for YBR134W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2584	androsterone	18.50	48.7 µM	3.63E-83	cell wall	2	1.12E-76
2	SGTC_2582	agaric acid	15.60	100.0 µM	6.96E-60	redox potentiating	12	2.96E-55
3	SGTC_514	4466-0038	14.50	2.6 nM	3.66E-52	cell wall	9	3.76E-48
4	SGTC_58	0957-0393	6.68	9.0 µM	1.65E-12	cell wall	176	1.19E-11
5	SGTC_2025	5147754	6.09	141.0 µM	1.12E-10		1	5.80E-10
6	SGTC_2030	5175110	5.57	200.0 µM	3.25E-9	Golgi	8	1.30E-8
7	SGTC_2989	9073649	5.38	71.4 µM	9.98E-9		11	3.64E-8
8	SGTC_304	1310-0298	5.35	97.0 µM	1.22E-8	cell wall	26	4.40E-8
9	SGTC_1689	st020265	5.22	53.5 µM	2.65E-8		5	8.95E-8
10	SGTC_2042	p-Dimethylaminoacetophenone	5.16	200.0 µM	3.74E-8		4	1.23E-7
11	SGTC_1749	st037932	5.15	54.2 µM	4.02E-8		15	1.32E-7
12	SGTC_2083	5306046	4.94	65.9 µM	1.34E-7		8	3.98E-7
13	SGTC_138	0850-0259	4.89	40.9 µM	1.68E-7	cell wall	146	4.93E-7
14	SGTC_1508	1080-0625	4.71	72.9 µM	4.46E-7		8	1.21E-6
15	SGTC_1378	2093-0007	4.64	380.0 nM	6.60E-7		8	1.74E-6
16	SGTC_1741	st038134	4.58	10.2 µM	9.18E-7		18	2.36E-6
17	SGTC_596	1155-0152	4.52	116.0 µM	1.26E-6	TRP & mitochondrial translation	24	3.16E-6
18	SGTC_1243	0416-0003	4.49	5.7 µM	1.45E-6	PDR1	15	3.59E-6
19	SGTC_410	α-linolenic acid	4.49	100.0 µM	1.47E-6	excess fatty acid	24	3.65E-6
20	SGTC_1415	3966-0071	4.48	78.1 µM	1.48E-6	DNA intercalators	5	3.67E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.495	2.49E-207	YOL003C	PFA4	hom	Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.393	5.44E-124	YNL106C	INP52	hom	Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain
0.379	5.16E-115	YLL032C	YLL032C	hom	Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.319	3.38E-80	YBL085W	BOI1	hom	Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
0.313	4.91E-77	YBR020W	GAL1	hom	Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication
0.296	1.02E-68	YJL099W	CHS6	hom	Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.295	1.51E-68	YML103C	NUP188	hom	Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.294	8.82E-68	YJR142W_p	YJR142W_p	hom	Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.292	5.81E-67	YPL115C	BEM3	hom	Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
0.292	8.40E-67	YLR102C	APC9	hom	Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
0.285	1.44E-63	YCL005W	LDB16	hom	Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
0.284	2.10E-63	YBR023C	CHS3	hom	Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.279	7.77E-61	YJR054W	KCH1	hom	Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.273	3.25E-58	YMR124W_p	YMR124W_p	hom	Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.270	2.46E-57	YGL033W	HOP2	hom	Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair