HIPHOP chemogenomics database

YBR163W / EXO5

Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2

Zygosity: Homozygous strain

fixedexpanded
Profile for YBR163W / EXO5

Click on Significant Values for Screen Details

Top fitness defect scores for YBR163W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_173	1273-0061	11.40	2.7 µM	9.67E-27	mitochondrial stress	29	1.48E-30
2	SGTC_3054	9094397	8.41	49.5 µM	2.30E-15	fatty acid desaturase (OLE1)	2	2.12E-17
3	SGTC_1022	4477-0945	7.33	21.3 µM	4.02E-12		2	1.17E-13
4	SGTC_236	1509-0011	6.91	5.3 µM	5.65E-11		269	2.45E-12
5	SGTC_1921	5740136	6.41	50.0 µM	1.07E-9	TRP & mitochondrial translation	12	7.22E-11
6	SGTC_2760	etidronate	5.93	98.0 µM	1.48E-8		3	1.49E-9
7	SGTC_3238	9133470	5.78	49.5 µM	3.28E-8		2	3.72E-9
8	SGTC_2704	st078022	5.67	71.1 µM	5.71E-8		5	7.05E-9
9	SGTC_310	1273-0058	5.58	1.1 µM	8.92E-8	mitochondrial stress	155	1.18E-8
10	SGTC_28	pentachloropyridine N-oxide	5.52	1.6 µM	1.21E-7	TSC3-RPN4	23	1.68E-8
11	SGTC_10	0279-0212	5.44	42.6 µM	1.80E-7	TRP & mitochondrial translation	41	2.63E-8
12	SGTC_997	3996-0078	5.21	137.0 µM	5.59E-7		5	9.67E-8
13	SGTC_3150	9097574	5.19	49.5 µM	6.12E-7		3	1.07E-7
14	SGTC_745	0469-0705	5.03	296.0 µM	1.27E-6	mitochondrial processes	8	2.47E-7
15	SGTC_581	0958-0271	5.00	211.0 µM	1.47E-6		26	2.93E-7
16	SGTC_100	0086-0128	4.95	49.6 µM	1.84E-6	copper-dependent oxidative stress	24	3.79E-7
17	SGTC_2921	7974240	4.94	10.7 µM	1.85E-6		27	3.83E-7
18	SGTC_99	0109-0120	4.94	622.8 µM	1.88E-6		2	3.89E-7
19	SGTC_118	0083-0097	4.90	135.3 µM	2.30E-6	translation	65	4.91E-7
20	SGTC_2250	alverine citrate	4.83	130.0 µM	3.02E-6		103	6.70E-7

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.301	2.53E-71	YPL040C	ISM1	hom	Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth
0.295	1.93E-68	YER087W	AIM10	hom	Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss
0.289	1.29E-65	YMR097C	MTG1	hom	Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals
0.287	9.24E-65	YDR296W	MHR1	hom	Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress
0.282	2.23E-62	YGL064C	MRH4	hom	Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function
0.276	1.73E-59	YMR267W	PPA2	hom	Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate
0.274	5.42E-59	YDR519W	FPR2	hom	Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress
0.274	1.12E-58	YLR067C	PET309	hom	Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs)
0.273	2.99E-58	YLR091W	GEP5	hom	Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine
0.264	1.26E-54	YHR120W	MSH1	hom	DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis
0.264	1.67E-54	YDL202W	MRPL11	hom	Mitochondrial ribosomal protein of the large subunit
0.264	1.67E-54	YMR228W	MTF1	hom	Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
0.263	2.49E-54	YMR193W	MRPL24	hom	Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene
0.263	5.43E-54	YDR462W	MRPL28	hom	Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
0.262	6.19E-54	YDR065W	RRG1	hom	Protein of unknown function, required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies