YBR176W / ECM31

Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR176W / ECM31

Click on Significant Values for Screen Details ID:SGTC_150|Compound:3970-0969|FD-Score:-3.56|P-value:1.75E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:-3.19|P-value:6.83E-4 ID:SGTC_328|Compound:k915-0120|FD-Score:-3.54|P-value:1.93E-4 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:3.61|P-value:1.82E-4 ID:SGTC_717|Compound:1532-0246|FD-Score:3.41|P-value:3.77E-4 ID:SGTC_732|Compound:3970-1727|FD-Score:-3.13|P-value:8.50E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:-4.25|P-value:1.06E-5 ID:SGTC_828|Compound:2074-0507|FD-Score:-3.30|P-value:4.70E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:3.29|P-value:5.88E-4 ID:SGTC_1158|Compound:4092-0494|FD-Score:-3.61|P-value:1.50E-4 ID:SGTC_1160|Compound:4092-0570|FD-Score:4.44|P-value:5.62E-6 ID:SGTC_1202|Compound:1988-0068|FD-Score:-3.19|P-value:6.88E-4 ID:SGTC_1296|Compound:1068-0102|FD-Score:-3.75|P-value:8.47E-5 ID:SGTC_1302|Compound:1159-0335|FD-Score:3.59|P-value:1.98E-4 ID:SGTC_1314|Compound:1222-0010|FD-Score:-3.82|P-value:6.36E-5 ID:SGTC_1445|Compound:4239-0156|FD-Score:-3.08|P-value:9.89E-4 ID:SGTC_1456|Compound:k015-0012|FD-Score:-3.75|P-value:8.41E-5 ID:SGTC_1519|Compound:esterone|FD-Score:3.25|P-value:6.73E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:4.00|P-value:3.88E-5 ID:SGTC_1819|Compound:st053448|FD-Score:3.72|P-value:1.17E-4 ID:SGTC_1894|Compound:3',6'-dihydroxyflavone|FD-Score:-3.15|P-value:7.77E-4 ID:SGTC_2005|Compound:4024620|FD-Score:3.16|P-value:9.02E-4 ID:SGTC_2079|Compound:5212404|FD-Score:3.18|P-value:8.49E-4 ID:SGTC_2331|Compound:7980721|FD-Score:-3.10|P-value:9.19E-4 ID:SGTC_2430|Compound:5571206|FD-Score:-3.64|P-value:1.31E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.51|P-value:2.63E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:-3.62|P-value:1.43E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:-4.19|P-value:1.36E-5 ID:SGTC_2591|Compound:skatole|FD-Score:3.35|P-value:4.69E-4 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:3.15|P-value:9.46E-4 ID:SGTC_2763|Compound:lisinopril|FD-Score:-3.10|P-value:9.15E-4 ID:SGTC_2796|Compound:7949634|FD-Score:-3.19|P-value:6.93E-4 ID:SGTC_2810|Compound:7959871|FD-Score:3.16|P-value:9.21E-4 ID:SGTC_2829|Compound:7992743|FD-Score:-3.43|P-value:2.94E-4 ID:SGTC_3144|Compound:9087057|FD-Score:-3.24|P-value:5.77E-4 ID:SGTC_3273|Compound:9137859|FD-Score:3.19|P-value:8.14E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:-3.56|P-value:1.75E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:-3.19|P-value:6.83E-4 ID:SGTC_328|Compound:k915-0120|FD-Score:-3.54|P-value:1.93E-4 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:3.61|P-value:1.82E-4 ID:SGTC_717|Compound:1532-0246|FD-Score:3.41|P-value:3.77E-4 ID:SGTC_732|Compound:3970-1727|FD-Score:-3.13|P-value:8.50E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:-4.25|P-value:1.06E-5 ID:SGTC_828|Compound:2074-0507|FD-Score:-3.30|P-value:4.70E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:3.29|P-value:5.88E-4 ID:SGTC_1158|Compound:4092-0494|FD-Score:-3.61|P-value:1.50E-4 ID:SGTC_1160|Compound:4092-0570|FD-Score:4.44|P-value:5.62E-6 ID:SGTC_1202|Compound:1988-0068|FD-Score:-3.19|P-value:6.88E-4 ID:SGTC_1296|Compound:1068-0102|FD-Score:-3.75|P-value:8.47E-5 ID:SGTC_1302|Compound:1159-0335|FD-Score:3.59|P-value:1.98E-4 ID:SGTC_1314|Compound:1222-0010|FD-Score:-3.82|P-value:6.36E-5 ID:SGTC_1445|Compound:4239-0156|FD-Score:-3.08|P-value:9.89E-4 ID:SGTC_1456|Compound:k015-0012|FD-Score:-3.75|P-value:8.41E-5 ID:SGTC_1519|Compound:esterone|FD-Score:3.25|P-value:6.73E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:4.00|P-value:3.88E-5 ID:SGTC_1819|Compound:st053448|FD-Score:3.72|P-value:1.17E-4 ID:SGTC_1894|Compound:3',6'-dihydroxyflavone|FD-Score:-3.15|P-value:7.77E-4 ID:SGTC_2005|Compound:4024620|FD-Score:3.16|P-value:9.02E-4 ID:SGTC_2079|Compound:5212404|FD-Score:3.18|P-value:8.49E-4 ID:SGTC_2331|Compound:7980721|FD-Score:-3.10|P-value:9.19E-4 ID:SGTC_2430|Compound:5571206|FD-Score:-3.64|P-value:1.31E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.51|P-value:2.63E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:-3.62|P-value:1.43E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:-4.19|P-value:1.36E-5 ID:SGTC_2591|Compound:skatole|FD-Score:3.35|P-value:4.69E-4 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:3.15|P-value:9.46E-4 ID:SGTC_2763|Compound:lisinopril|FD-Score:-3.10|P-value:9.15E-4 ID:SGTC_2796|Compound:7949634|FD-Score:-3.19|P-value:6.93E-4 ID:SGTC_2810|Compound:7959871|FD-Score:3.16|P-value:9.21E-4 ID:SGTC_2829|Compound:7992743|FD-Score:-3.43|P-value:2.94E-4 ID:SGTC_3144|Compound:9087057|FD-Score:-3.24|P-value:5.77E-4 ID:SGTC_3273|Compound:9137859|FD-Score:3.19|P-value:8.14E-4

Top fitness defect scores for YBR176W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1160 4092-0570 4.44 10.5 µM 5.62E-6 plasma membrane duress 28 4.50E-6
2 SGTC_1529 apigenin 4.00 74.0 µM 3.88E-5 33 3.19E-5
3 SGTC_1819 st053448 3.72 58.6 µM 1.17E-4 amide catabolism 25 9.80E-5
4 SGTC_475 α-linolenic acid 3.61 10.0 µM 1.82E-4 excess fatty acid 24 1.53E-4
5 SGTC_1302 1159-0335 3.59 255.0 µM 1.98E-4 21 1.67E-4
6 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 3.51 3.7 µM 2.63E-4 125 2.24E-4
7 SGTC_717 1532-0246 3.41 73.2 µM 3.77E-4 Golgi 54 3.21E-4
8 SGTC_2591 skatole 3.35 100.0 µM 4.69E-4 45 4.02E-4
9 SGTC_963 1269-2853 3.29 151.0 µM 5.88E-4 48 5.06E-4
10 SGTC_1519 esterone 3.25 74.0 µM 6.73E-4 DNA intercalators 46 5.80E-4
11 SGTC_3273 9137859 3.19 49.5 µM 8.14E-4 18 7.04E-4
12 SGTC_2079 5212404 3.18 121.0 µM 8.49E-4 27 7.34E-4
13 SGTC_2005 4024620 3.16 188.0 µM 9.02E-4 54 7.81E-4
14 SGTC_2810 7959871 3.16 45.5 µM 9.21E-4 copper-dependent oxidative stress 78 7.98E-4
15 SGTC_2598 perillyl alcohol 3.15 100.0 µM 9.46E-4 71 8.19E-4
16 SGTC_3353 9151644 3.09 39.3 µM 0.00113 44 9.86E-4
17 SGTC_2019 4016004 3.06 108.0 µM 0.00129 68 0.00112
18 SGTC_2950 9072895 3.03 21.5 µM 0.00138 92 0.00120
19 SGTC_2606 farnesol 3.02 9.8 µM 0.00145 68 0.00126
20 SGTC_1957 st077035 2.99 62.4 µM 0.00160 48 0.00140

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.153 4.64E-19 YMR080C NAM7 hom ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress
0.102 3.55E-9 YKL215C OXP1 hom 5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
0.101 4.79E-9 YHR086W-A_p YHR086W-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.101 5.45E-9 YHR139C SPS100 hom Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin
0.095 3.17E-8 YLR328W NMA1 hom Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication
0.089 2.23E-7 YDR452W PPN1 hom Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress
0.084 1.02E-6 YLR354C TAL1 hom Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate
0.080 3.89E-6 YOR356W CIR2 hom Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response
0.078 6.62E-6 YFL034C-A RPL22B hom Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication
0.078 7.05E-6 YIR013C GAT4 hom Protein containing GATA family zinc finger motifs
0.077 8.26E-6 YDR445C_d YDR445C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.075 1.26E-5 YFL008W SMC1 het Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure
0.075 1.30E-5 YDR081C PDC2 het Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
0.075 1.45E-5 YMR105W-A_p YMR105W-A_p hom Putative protein of unknown function
0.075 1.50E-5 YLR120C YPS1 hom Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor