YBR193C / MED8

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation

Zygosity: Heterozygous strain
fixedexpanded
Profile for YBR193C / MED8

Click on Significant Values for Screen Details ID:SGTC_128|Compound:0906-2981|FD-Score:3.59|P-value:8.70E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:3.44|P-value:1.61E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:9.29|P-value:1.17E-22 ID:SGTC_324|Compound:1315-0088|FD-Score:4.23|P-value:4.88E-6 ID:SGTC_366|Compound:0181-0206|FD-Score:-3.34|P-value:2.39E-4 ID:SGTC_529|Compound:4076-0302|FD-Score:-3.05|P-value:7.05E-4 ID:SGTC_530|Compound:4358-1714|FD-Score:3.78|P-value:3.84E-5 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:3.46|P-value:1.48E-4 ID:SGTC_595|Compound:3909-8003|FD-Score:-3.01|P-value:8.06E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:3.55|P-value:1.00E-4 ID:SGTC_671|Compound:0560-0003|FD-Score:-3.17|P-value:4.54E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:4.21|P-value:5.28E-6 ID:SGTC_806|Compound:3966-4455|FD-Score:3.91|P-value:2.15E-5 ID:SGTC_859|Compound:0439-0102|FD-Score:-3.84|P-value:2.92E-5 ID:SGTC_966|Compound:1300-0360|FD-Score:3.04|P-value:7.35E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:-3.42|P-value:1.74E-4 ID:SGTC_1152|Compound:3909-8331|FD-Score:3.97|P-value:1.64E-5 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.67|P-value:5.06E-7 ID:SGTC_1163|Compound:k060-0048|FD-Score:-3.06|P-value:6.74E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:3.20|P-value:4.02E-4 ID:SGTC_1396|Compound:3511-0007|FD-Score:3.47|P-value:1.43E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:-3.44|P-value:1.58E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:4.29|P-value:3.51E-6 ID:SGTC_1463|Compound:k081-0032|FD-Score:3.20|P-value:4.07E-4 ID:SGTC_1531|Compound:st059297|FD-Score:-3.39|P-value:1.95E-4 ID:SGTC_1934|Compound:st074720|FD-Score:3.71|P-value:5.06E-5 ID:SGTC_2028|Compound:5153890|FD-Score:-4.12|P-value:8.24E-6 ID:SGTC_2033|Compound:5192550|FD-Score:2.99|P-value:8.70E-4 ID:SGTC_2047|Compound:4141938|FD-Score:3.26|P-value:3.21E-4 ID:SGTC_2240|Compound:6690706|FD-Score:5.07|P-value:5.74E-8 ID:SGTC_2307|Compound:7538926|FD-Score:3.29|P-value:2.84E-4 ID:SGTC_2310|Compound:7668677|FD-Score:6.85|P-value:3.90E-13 ID:SGTC_2320|Compound:7373003|FD-Score:-3.78|P-value:3.80E-5 ID:SGTC_2418|Compound:pentamidine|FD-Score:3.12|P-value:5.51E-4 ID:SGTC_2431|Compound:5490079|FD-Score:-3.63|P-value:7.18E-5 ID:SGTC_2516|Compound:gossypol|FD-Score:4.84|P-value:2.06E-7 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.50|P-value:1.24E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:-3.18|P-value:4.43E-4 ID:SGTC_2668|Compound:2',4'-dihydroxychalcone|FD-Score:3.28|P-value:3.02E-4 ID:SGTC_2740|Compound:felodipine|FD-Score:2.99|P-value:8.63E-4 ID:SGTC_2785|Compound:7756463|FD-Score:5.19|P-value:2.85E-8 ID:SGTC_2804|Compound:7954240|FD-Score:3.36|P-value:2.18E-4 ID:SGTC_2848|Compound:9016370|FD-Score:-3.44|P-value:1.56E-4 ID:SGTC_3015|Compound:9080845|FD-Score:2.98|P-value:9.08E-4 ID:SGTC_3029|Compound:9088683|FD-Score:-3.08|P-value:6.26E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-12.40|P-value:7.51E-39 ID:SGTC_3063|Compound:1327-0034|FD-Score:4.48|P-value:1.37E-6 ID:SGTC_3067|Compound:9117431|FD-Score:3.08|P-value:6.29E-4 ID:SGTC_3090|Compound:9117696|FD-Score:-3.01|P-value:8.18E-4 ID:SGTC_3091|Compound:9116372|FD-Score:-4.19|P-value:5.91E-6 ID:SGTC_3139|Compound:9095020|FD-Score:6.83|P-value:4.46E-13 ID:SGTC_3145|Compound:9085516|FD-Score:6.33|P-value:1.71E-11 ID:SGTC_3154|Compound:9100113|FD-Score:4.04|P-value:1.15E-5 ID:SGTC_3213|Compound:9129884|FD-Score:4.01|P-value:1.36E-5 ID:SGTC_3232|Compound:9132874|FD-Score:4.71|P-value:4.08E-7 ID:SGTC_3285|Compound:9112818|FD-Score:3.10|P-value:5.92E-4 ID:SGTC_3300|Compound:9124615|FD-Score:4.19|P-value:5.83E-6 ID:SGTC_3341|Compound:9147222|FD-Score:3.11|P-value:5.57E-4 ID:SGTC_128|Compound:0906-2981|FD-Score:3.59|P-value:8.70E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:3.44|P-value:1.61E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:9.29|P-value:1.17E-22 ID:SGTC_324|Compound:1315-0088|FD-Score:4.23|P-value:4.88E-6 ID:SGTC_366|Compound:0181-0206|FD-Score:-3.34|P-value:2.39E-4 ID:SGTC_529|Compound:4076-0302|FD-Score:-3.05|P-value:7.05E-4 ID:SGTC_530|Compound:4358-1714|FD-Score:3.78|P-value:3.84E-5 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:3.46|P-value:1.48E-4 ID:SGTC_595|Compound:3909-8003|FD-Score:-3.01|P-value:8.06E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:3.55|P-value:1.00E-4 ID:SGTC_671|Compound:0560-0003|FD-Score:-3.17|P-value:4.54E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:4.21|P-value:5.28E-6 ID:SGTC_806|Compound:3966-4455|FD-Score:3.91|P-value:2.15E-5 ID:SGTC_859|Compound:0439-0102|FD-Score:-3.84|P-value:2.92E-5 ID:SGTC_966|Compound:1300-0360|FD-Score:3.04|P-value:7.35E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:-3.42|P-value:1.74E-4 ID:SGTC_1152|Compound:3909-8331|FD-Score:3.97|P-value:1.64E-5 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.67|P-value:5.06E-7 ID:SGTC_1163|Compound:k060-0048|FD-Score:-3.06|P-value:6.74E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:3.20|P-value:4.02E-4 ID:SGTC_1396|Compound:3511-0007|FD-Score:3.47|P-value:1.43E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:-3.44|P-value:1.58E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:4.29|P-value:3.51E-6 ID:SGTC_1463|Compound:k081-0032|FD-Score:3.20|P-value:4.07E-4 ID:SGTC_1531|Compound:st059297|FD-Score:-3.39|P-value:1.95E-4 ID:SGTC_1934|Compound:st074720|FD-Score:3.71|P-value:5.06E-5 ID:SGTC_2028|Compound:5153890|FD-Score:-4.12|P-value:8.24E-6 ID:SGTC_2033|Compound:5192550|FD-Score:2.99|P-value:8.70E-4 ID:SGTC_2047|Compound:4141938|FD-Score:3.26|P-value:3.21E-4 ID:SGTC_2240|Compound:6690706|FD-Score:5.07|P-value:5.74E-8 ID:SGTC_2307|Compound:7538926|FD-Score:3.29|P-value:2.84E-4 ID:SGTC_2310|Compound:7668677|FD-Score:6.85|P-value:3.90E-13 ID:SGTC_2320|Compound:7373003|FD-Score:-3.78|P-value:3.80E-5 ID:SGTC_2418|Compound:pentamidine|FD-Score:3.12|P-value:5.51E-4 ID:SGTC_2431|Compound:5490079|FD-Score:-3.63|P-value:7.18E-5 ID:SGTC_2516|Compound:gossypol|FD-Score:4.84|P-value:2.06E-7 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.50|P-value:1.24E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:-3.18|P-value:4.43E-4 ID:SGTC_2668|Compound:2',4'-dihydroxychalcone|FD-Score:3.28|P-value:3.02E-4 ID:SGTC_2740|Compound:felodipine|FD-Score:2.99|P-value:8.63E-4 ID:SGTC_2785|Compound:7756463|FD-Score:5.19|P-value:2.85E-8 ID:SGTC_2804|Compound:7954240|FD-Score:3.36|P-value:2.18E-4 ID:SGTC_2848|Compound:9016370|FD-Score:-3.44|P-value:1.56E-4 ID:SGTC_3015|Compound:9080845|FD-Score:2.98|P-value:9.08E-4 ID:SGTC_3029|Compound:9088683|FD-Score:-3.08|P-value:6.26E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-12.40|P-value:7.51E-39 ID:SGTC_3063|Compound:1327-0034|FD-Score:4.48|P-value:1.37E-6 ID:SGTC_3067|Compound:9117431|FD-Score:3.08|P-value:6.29E-4 ID:SGTC_3090|Compound:9117696|FD-Score:-3.01|P-value:8.18E-4 ID:SGTC_3091|Compound:9116372|FD-Score:-4.19|P-value:5.91E-6 ID:SGTC_3139|Compound:9095020|FD-Score:6.83|P-value:4.46E-13 ID:SGTC_3145|Compound:9085516|FD-Score:6.33|P-value:1.71E-11 ID:SGTC_3154|Compound:9100113|FD-Score:4.04|P-value:1.15E-5 ID:SGTC_3213|Compound:9129884|FD-Score:4.01|P-value:1.36E-5 ID:SGTC_3232|Compound:9132874|FD-Score:4.71|P-value:4.08E-7 ID:SGTC_3285|Compound:9112818|FD-Score:3.10|P-value:5.92E-4 ID:SGTC_3300|Compound:9124615|FD-Score:4.19|P-value:5.83E-6 ID:SGTC_3341|Compound:9147222|FD-Score:3.11|P-value:5.57E-4

Top fitness defect scores for YBR193C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_168 3970-0862 9.29 530.5 µM 1.17E-22 3 7.56E-21 0.41
2 SGTC_2310 7668677 6.85 200.0 µM 3.90E-13 1 3.82E-12 1.78
3 SGTC_3139 9095020 6.83 49.5 µM 4.46E-13 1 4.32E-12 2.36
4 SGTC_3145 9085516 6.33 49.5 µM 1.71E-11 fatty acid desaturase (OLE1) 3 1.21E-10 0.82
5 SGTC_2785 7756463 5.19 72.7 µM 2.85E-8 6 1.07E-7 0.32
6 SGTC_2240 6690706 5.07 185.9 µM 5.74E-8 3 2.04E-7 0.75
7 SGTC_2516 gossypol 4.84 71.5 µM 2.06E-7 7 6.57E-7 0.07
8 SGTC_3232 9132874 4.71 49.5 µM 4.08E-7 RPP1 & pyrimidine depletion 5 1.23E-6 0.50
9 SGTC_1161 k007-0610 4.67 110.0 µM 5.06E-7 ERAD & cell cycle 27 1.50E-6 0.01
10 SGTC_3063 1327-0034 4.48 4.0 µM 1.37E-6 7 3.71E-6 0.23
11 SGTC_1462 k081-0014 4.29 26.5 µM 3.51E-6 2 8.82E-6 0.78
12 SGTC_324 1315-0088 4.23 19.6 µM 4.88E-6 RSC & ERG11 10 1.19E-5 0.11
13 SGTC_673 1082-0474 4.21 110.0 µM 5.28E-6 RSC complex & mRNA processing 47 1.28E-5 0.00
14 SGTC_3300 9124615 4.19 50.5 µM 5.83E-6 1 1.40E-5 0.74
15 SGTC_3154 9100113 4.04 49.5 µM 1.15E-5 11 2.62E-5 0.16
16 SGTC_3213 9129884 4.01 49.5 µM 1.36E-5 2 3.05E-5 0.28
17 SGTC_1152 3909-8331 3.97 15.9 µM 1.64E-5 6 3.62E-5 0.55
18 SGTC_806 3966-4455 3.91 5.9 µM 2.15E-5 7 4.65E-5 0.19
19 SGTC_530 4358-1714 3.78 28.0 µM 3.84E-5 8 7.90E-5 0.07
20 SGTC_1934 st074720 3.71 32.6 µM 5.06E-5 16 1.02E-4 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.185 2.81E-27 YCR013C_d YCR013C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant
0.166 3.78E-22 YJL101C GSH1 hom Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
0.162 2.98E-21 YER018C SPC25 het Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
0.151 1.63E-18 YKL083W_d YKL083W_d het Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
0.141 1.76E-16 YHR197W RIX1 het Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene
0.141 2.08E-16 YNL151C RPC31 het RNA polymerase III subunit C31
0.135 4.01E-15 YER147C SCC4 het Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX
0.131 2.09E-14 YBL004W UTP20 het Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
0.129 6.80E-14 YER172C BRR2 het RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD
0.128 9.25E-14 YDR005C MAF1 hom Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions
0.126 1.98E-13 YOR335C ALA1 het Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog
0.126 2.51E-13 YMR288W HSH155 het U2-snRNP associated splicing factor that forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155
0.125 3.12E-13 YGL142C GPI10 het Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog
0.125 3.51E-13 YML098W TAF13 het TFIID subunit (19 kDa), involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors
0.123 1.03E-12 YML046W PRP39 het U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats